RESUMEN
The capacity to identify unanticipated abnormal cues in a natural scene is vital for animal survival. Stimulus-specific adaptation (SSA) has been considered the neuronal correlate for deviance detection. There have been comprehensive assessments of SSA in the frequency domain along the ascending auditory pathway, but only little attention given to deviance detection in the spatial domain. We found that thalamic reticular nucleus (TRN) neurons exhibited stronger responses to a tone when it was presented rarely as opposed to frequently at a certain spatial location. Subsequently, we engaged signal detection theory to directly gauge neuronal spatial discriminability and found that discrimination of deviant locations was considerably higher than standard locations. The variability in neuronal spatial discriminability among the TRN population was directly related to response difference (RD) but not variance; meanwhile, further analyses attributed higher spatial sensitivity at deviant locations to larger RD. Astonishingly, a significant correlation was found between the amount of adaptation and deviant discriminability. Collectively, our results suggest that adaptation facilitates rare location discrimination by sharpening the response gap between two locations.
Asunto(s)
Adaptación Fisiológica/fisiología , Vías Auditivas/fisiología , Discriminación en Psicología/fisiología , Percepción Espacial/fisiología , Núcleos Talámicos/fisiología , Estimulación Acústica , Potenciales de Acción/fisiología , Animales , Percepción Auditiva , Femenino , Masculino , Neuronas/fisiología , Curva ROC , Ratas , Ratas Wistar , Núcleos Talámicos/citologíaRESUMEN
AIM: The roles of G-protein coupled receptors (GPCRs) in stem cell biology remain unclear. In this study, we aimed to identify GPCRs that might contribute to the self-renewal of mouse embryonic stem cells (mESCs). METHODS: The expression levels of pluripotent genes and GPCR gene were detected in E14 mESCs using PCR array and RT-PCR. Immunofluorescent staining was used to examine the expression of pluripotent markers and the receptor translocation. Western blot analysis was used to detect phosphorylation of signal proteins. Knock-down of receptor was conducted to confirm its role in pluripotency maintenance. RESULTS: In leukemia inhibitory factor (LIF)-free medium, mESCs lost the typical morphology of pluripotency, accompanied by markedly decreases in expression of somatostatin receptor type 2 (SSTR2), as well as the pluripotency biomarkers Oct4, Sox2, Rex1 and Nanog. Addition of the SSTR2 agonist octreotide or seglitide (0.1-30 µmol/L) in LIF-free medium dose-dependently promoted the self-renewal of mESCs, whereas the SSTR2 antagonist S4 (0.03-3 µmol/L) dose-dependently blocked octreotide-induced self-renewal. Knock-down of SSTR2 significantly decreased the self-renewal of mESCs even in the presence of LIF. Addition of LIF (1000 U/mL) or octreotide (1 µmol/L) in LIF-free medium significantly increased both phosphorylation and nuclear ocalization of STAT3. CONCLUSION: The activation of SSTR2 contributes to the self-renewal of mESCs via activation of the STAT3 pathway.
Asunto(s)
Células Madre Embrionarias/citología , Receptores de Somatostatina/genética , Animales , Diferenciación Celular , Línea Celular , Proliferación Celular , Células Madre Embrionarias/metabolismo , Regulación del Desarrollo de la Expresión Génica , Técnicas de Silenciamiento del Gen , Factor Inhibidor de Leucemia/metabolismo , Ratones , Receptores de Somatostatina/metabolismo , Factor de Transcripción STAT3/metabolismo , Transducción de SeñalRESUMEN
The thalamic reticular nucleus (TRN) is thought to function in the attentional searchlight. We analyzed the detection of deviant acoustic stimuli by TRN neurons and the consequences of deviance detection on the TRN target, the medial geniculate body (MGB) of the rat. TRN neurons responded more strongly to pure-tone stimuli presented as deviant stimuli (low appearance probability) than those presented as standard stimuli (high probability) (deviance-detection index = 0.321). MGB neurons also showed deviance detection in this procedure, albeit to a smaller extent (deviance-detection index = 0.154). TRN neuron deviance detection either enhanced (14 neurons) or suppressed (27 neurons) MGB neuronal responses to a probe stimulus. Both effects were neutralized by inactivation of the auditory TRN. Deviance modulation effects were cross-modal. Deviance detection probably causes TRN neurons to transiently deactivate surrounding TRN neurons in response to a fresh stimulus, altering auditory thalamus responses and inducing attention shift.
Asunto(s)
Percepción Auditiva/fisiología , Cuerpos Geniculados/fisiología , Neuronas/fisiología , Detección de Señal Psicológica/fisiología , Núcleos Talámicos/fisiología , Percepción Visual/fisiología , Estimulación Acústica , Potenciales de Acción , Análisis de Varianza , Animales , Femenino , Masculino , Microelectrodos , Pruebas Neuropsicológicas , Estimulación Luminosa , Probabilidad , Ratas , Ratas WistarRESUMEN
Responses to repeated auditory stimuli were examined in 103 neurons in the auditory region of the thalamic reticular nucleus (TRN) and in 20 medial geniculate (MGB) neurons of anesthetized rats. A further six TRN neurons were recorded from awake rats. The TRN neurons showed strong responses to the first trial and weak responses to the subsequent trials of repeated auditory stimuli and electrical stimulation of the MGB and auditory cortex when the interstimulus interval (ISI) was short (<3 s). They responded to the second trial when the interstimulus interval was lengthened to >or=3 s. These responses contrasted to those of MGB neurons, which responded to repeated auditory stimuli of different ISIs. The TRN neurons showed a significant increase in the onset auditory response from 9.5 to 76.5 Hz when the ISI was increased from 200 ms to 10 s (P<0.001, ANOVA). The duration of the auditory-evoked oscillation was longer when the ISI was lengthened. The slow recovery of the TRN neurons after oscillation of burst firings to fast repetitive stimulus was a reflection of a different role than that of the thalamocortical relay neurons. Supposedly the TRN is involved in the process of attention such as attention shift; the slow recovery of TRN neurons probably limits the frequent change of the attention in a fast rhythm.