RESUMEN
A new furostanol saponin, (25S)-26-O-ß-D-glucopyranosyl-5ß-furost-20(22)-en-3ß, 15ß,26-triol-3-O-[α-L-rhamnopyranosyl-(1-4)]-ß-D: -glucopyranoside, namely, aspacochioside D (1) were isolated from Asparagus cochinchinensis (Lour.) Merr, along with three known saponins, aspacochioside C (2), (25S)-5ß-spirostan-3ß-yl-O-[O-α-L-rhamnopyranosyl-(1-4)]-ß-D-glucopyranoside (3), and pseudoprotoneodioscin (4). The structure of 1 was elucidated on the basis of chemical reactions and spectral analysis (IR, GC, ESI-MS, (1)H-NMR, (13)C-NMR, DEPT, HMBC, HMQC and NOESY). The antiproliferative effects of 1-4 were evaluated in a cytotoxicity assay against the human tumor cell line, A549. Compound 2 (Aspacochioside C) exhibited moderate cytotoxicity against A-549, with an IC(50) value of 3.87 µg/mL.
Asunto(s)
Antineoplásicos Fitogénicos/química , Asparagus/química , Saponinas/química , Antineoplásicos Fitogénicos/aislamiento & purificación , Antineoplásicos Fitogénicos/farmacología , Línea Celular Tumoral , Cromatografía de Gases , Colorantes , Ensayos de Selección de Medicamentos Antitumorales , Humanos , Hidrólisis , Espectroscopía de Resonancia Magnética , Extractos Vegetales/química , Extractos Vegetales/farmacología , Raíces de Plantas/química , Saponinas/aislamiento & purificación , Saponinas/farmacología , Espectrometría de Masa por Ionización de Electrospray , Espectrofotometría Infrarroja , Esteroides/química , Esteroides/aislamiento & purificación , Esteroides/farmacología , Sales de Tetrazolio , TiazolesRESUMEN
OBJECTIVE: To investigate the technological parameters and the process for separation and purification of pumiloside (PML) from Nauclea officinalis Pierrc ex Pitard by macroreticular resin. METHODS: PML was extracted by hot water, and the content of PML was determinated by HPLC method. The static capacity absorption, static elution ratios of five types of resin were studied respectively, and were compared to evaluate their absorption and desorption effect to PML. And then the absorption capacity, elution solution and elution volume of the AB-8 resin were researched to set up the optimum separation process for PML. Finally, PML was recrysallized from MeOH and identified by spectra analysis. RESULTS: The AB-8 resin had the best absorptive and separative properties to PML. The dynamic absorption ratio was 2.44 mg/g. The applicable process was as follows: the water extracted solution of Nauclea officinalis flow through the resin column repeatedly, after being eluted with 6BV of distilled water, the resin column was eluted with 4BV 30% ethanol, the 30% ethanol fraction was combined and the solvent was recovered in vacuum. The precipitation was filtered and recrysallized from MeOH to give pure PML. The yield of PML was 75.1%, and the product purity was up to 99.5%. CONCLUSION: AB-8 resin shows better comprehensive absorption property. It can be used for separation and purification of PML from Nauclea officinalis successfully.
Asunto(s)
Alcaloides/aislamiento & purificación , Camptotecina/análogos & derivados , Plantas Medicinales/química , Resinas Sintéticas/química , Rubiaceae/química , Tecnología Farmacéutica/métodos , Adsorción , Alcaloides/química , Camptotecina/química , Camptotecina/aislamiento & purificación , Cromatografía Líquida de Alta Presión , Medicamentos Herbarios Chinos/química , Medicamentos Herbarios Chinos/aislamiento & purificación , Etanol , Reproducibilidad de los Resultados , AguaRESUMEN
AIM: To investigate the chemical constituents of water-soluble part of stem of Nauclea officinalis. METHODS: The compounds were isolated and purified by resins, silica gel, and Sephadex LH20 column chromatography repeatedly, and their structures were identified by spectral analysis. RESULTS: Seven compounds were isolated and identified as: naucleamide A-10-O-beta-D-glucopyranoside (I), 5-beta-carboxystrictosidin (II), sweroside (III), loganin (IV), 3,4-dimethoxyphenyl-beta-D-glucopyranoside (V), 3, 4, 5-trimethoxyphenyl-beta-D-glucopyranoside (VI), 2-phenethylrutinoside (VII). CONCLUSION: Compound I is a new indole alkaloid glucoside, compounds I-VII were isolated from this plant for the first time.
Asunto(s)
Carbolinas/química , Glucósidos/química , Glicósidos/química , Alcaloides Indólicos/química , Rubiaceae/química , Carbolinas/aislamiento & purificación , Glucósidos/aislamiento & purificación , Glicósidos/aislamiento & purificación , Alcaloides Indólicos/aislamiento & purificación , Glucósidos Iridoides , Iridoides/química , Iridoides/aislamiento & purificación , Conformación Molecular , Estructura Molecular , Tallos de la Planta/química , Plantas Medicinales/químicaRESUMEN
Seven lignans having a diarylhydroxybutyrolactone skeleton were isolated from the leaves and stems of Trachelospermum jasminoides (Lindl.) Lem. Their structures were elucidated to be nortrachelogenin 8' -O-beta-D-glucopyranoside (1), nortrachelogenin 5'- C- beta-D-glucopyranoside (2), trachelogenin amide (3), nortracheloside, trachelogenin, tracheloside, and trachelogenin 4'- O- beta-gentiobioside, respectively, on the basis of spectroscopic analyses. Lignans 1 - 3 were structurally identified to be new compounds, and 2 was a rare C-glucosyl-lignan.
Asunto(s)
Apocynaceae , Fitoterapia , Extractos Vegetales/química , Humanos , Lignanos/química , Hojas de la Planta , Tallos de la PlantaRESUMEN
OBJECTIVE: To study the chemical constituents of Ervatamia hainanensis. METHOD: The compounds were separated and purified by column chromatography with silica gel, and identified by IR, MS, NMR and 2D-NMR. RESULT: Five compounds were identified as I (isolariciresinol 9-O-beta-D-glucopyranoside), II (cycloartenol), III (beta-amyrin acetate), IV (beta-sitosterol), V (daucosterol), respectively. CONCLUSION: All the compounds were isolated from this plant for the first time.