RESUMEN
DNA comprises molecular information stored in genetic and epigenetic bases, both of which are vital to our understanding of biology. Most DNA sequencing approaches address either genetics or epigenetics and thus capture incomplete information. Methods widely used to detect epigenetic DNA bases fail to capture common C-to-T mutations or distinguish 5-methylcytosine from 5-hydroxymethylcytosine. We present a single base-resolution sequencing methodology that sequences complete genetics and the two most common cytosine modifications in a single workflow. DNA is copied and bases are enzymatically converted. Coupled decoding of bases across the original and copy strand provides a phased digital readout. Methods are demonstrated on human genomic DNA and cell-free DNA from a blood sample of a patient with cancer. The approach is accurate, requires low DNA input and has a simple workflow and analysis pipeline. Simultaneous, phased reading of genetic and epigenetic bases provides a more complete picture of the information stored in genomes and has applications throughout biomedicine.
RESUMEN
The pH-dependent activity of wild-type Bacillus circulans xylanase (BcX) is set by the pK(a) values of its nucleophile Glu78 and general acid/base Glu172. Herein, we examined several strategies to manipulate these pK(a) values and thereby shift the pH(opt) at which BcX is optimally active. Altering the global charge of BcX through random succinylation had no significant effect. Mutation of residues near or within the active site of BcX, but not directly contacting the catalytic carboxyls, either had little effect or reduced its pH(opt), primarily by lowering the apparent pK(a) value of Glu78. However, mutations causing the largest pK(a) changes also impaired activity. Although not found as a general acid/base in naturally occurring xylanases, substitution of Glu172 with a His lowered the pH(opt) of BcX from 5.6 to 4.7 while retaining 8% activity toward a xylobioside substrate. Mutation of Asn35, which contacts Glu172, to either His or Glu also led to a reduction in pH(opt) by ~1.2 units. Detailed pK(a) measurements by NMR spectroscopy revealed that, despite the opposite charges of the introduced residues, both the N35H and N35E forms of BcX utilize a reverse protonation mechanism. In this mechanism, the pK(a) value of the general acid is lower than that of the nucleophile, and only a small population of enzyme is in a catalytically competent ionization state. However, overall activity is maintained due to the increased strength of the general acid. This study illustrates several routes for altering the pH-dependent properties of xylanases, while also providing valuable insights into complex protein electrostatics.
Asunto(s)
Glicósido Hidrolasas/metabolismo , Concentración de Iones de Hidrógeno , Cristalografía por Rayos X , Glicósido Hidrolasas/química , Glicósido Hidrolasas/genética , Modelos Moleculares , Mutagénesis , Resonancia Magnética Nuclear BiomolecularRESUMEN
Photolyases and cryptochromes (CRY) are structurally homologous flavoproteins with divergent functions. While photolyases repair UV-damaged DNA by photoinduced electron transfer from their FAD cofactor, CRY are involved in varied cellular processes, including light-dependent plant growth, regulation of mammalian circadian rhythm, and possibly magnetoreception. Despite their importance in Nature and human health, little is known about how they tune their FAD redox properties to achieve remarkable functional diversity. In this study, we reveal a kinetic mechanism, exploited by cyclobutane pyrimidine dimer photolyase (PL), for regulating the stability of its FAD semiquinone (sq). We find that the sq in CRY-DASH (Synechocystis) is substantially more reactive toward oxidation than in PL (Anacystis nidulans) and, using deuterium isotope and pH effects, show that rate-limiting proton transfer contributes to the exceptional kinetic stability of the PL sq. Through mutagenesis, we identify two PL-specific residues in the flavin binding pocket, Trp392 and Gly389 (Try398 and Asn395 in CRY-DASH, respectively), that ensure this kinetic stability, possibly through interactions with the adenine moiety of FAD and/or adjusting the polarity of the binding site. Significantly, these relatively distal residues have a much more profound impact than two amino acids closer to the FAD. By quantifying sq stability in a series of PL-CRY exchange mutants, our findings pave the way for investigations aimed at correlating sq stability with function in these proteins. As is being recognized with other flavoproteins, we expect that kinetic tuning of the rates of electron transfer will play a function-defining role in photolyases and cryptochromes.
Asunto(s)
Criptocromos/metabolismo , Desoxirribodipirimidina Fotoliasa/metabolismo , Flavina-Adenina Dinucleótido/análogos & derivados , Ácido Aspártico/química , Ácido Aspártico/metabolismo , Sitios de Unión , Criptocromos/química , Cristalografía por Rayos X , Desoxirribodipirimidina Fotoliasa/química , Dimerización , Transporte de Electrón , Flavina-Adenina Dinucleótido/química , Flavina-Adenina Dinucleótido/metabolismo , Glicina/química , Glicina/metabolismo , Concentración de Iones de Hidrógeno , Cinética , Concentración Osmolar , Oxidación-Reducción , Triptófano/química , Triptófano/metabolismoRESUMEN
Hydrogen bonding plays a key role in the tight binding of the FMN cofactor and the regulation of its redox properties in flavodoxins. Hydrogen bonding interactions can be directly observed in solution by multidimensional heteronuclear NMR spectroscopy through the scalar couplings between donor and acceptor nuclei. Here we report on the detection of intermolecular trans-hydrogen-bond couplings ((h)J) between the flavin ring system and the backbone of Desulfovibrio vulgaris flavodoxin in the oxidized and the two-electron reduced states. For this purpose, experiments are adapted from pulse sequences previously applied to determining (h)J coupling constants in nucleic acid-base pairs and proteins. The resulting (h2)J(N,N), (h4)J(N,N), (h3)J(C,N), and (h1)J(H,N) couplings involve the (15)N(1), (13)C(2), and (15)N(3) nuclei of the pyrimidine moiety of FMN, whereas no such interactions are detectable for (13)C(4) and (15)N(5). Several long-range (15)N-(15)N, (13)C-(15)N, and (1)H-(15)N J-coupling constants within the flavin are obtained as "by-products". The magnitudes of both (h)J and regular J couplings are found to be dependent on the redox state. In general, good correlations between (h)J coupling constants and donor-group (1)H chemical shifts and also crystallographic donor-acceptor distances are observed.
Asunto(s)
Apoproteínas/química , Flavodoxina/química , Apoproteínas/metabolismo , Desulfovibrio vulgaris/metabolismo , Flavodoxina/metabolismo , Enlace de Hidrógeno , Modelos Moleculares , Estructura Molecular , Resonancia Magnética Nuclear Biomolecular/métodos , Oxidación-Reducción , Unión Proteica , Proteínas Recombinantes/química , Proteínas Recombinantes/metabolismo , Relación Estructura-ActividadRESUMEN
A quantitative J-correlation pulse sequence is described that allows simultaneous determination of one-bond and two-bond nitrogen-carbon coupling constants for protonated or deuterated proteins. Coupling constants are calculated from volume ratios between cross peaks and reference axial peaks observed in a single 3D spectrum. Accurate backbone (1)J(NC'), (1)J(NCalpha), and (2)J(NCalpha) coupling constants are obtained for the two [(15)N;(13)C]-labeled, medium-sized proteins flavodoxin and xylanase and for the [(2)H;(15)N;(13)C]-labeled, large protein DFPase. A dependence of one-bond and two-bond J(NCalpha) values on protein backbone psi torsion angles is readily apparent, in agreement with previously found correlations. In addition, the experiment is performed on isotropic as well as aligned protein to measure associated (15)N-(13)C residual dipolar couplings.
Asunto(s)
Proteínas/química , Xilosidasas/química , Bacillus/enzimología , Carbono , Isótopos de Carbono , Clonación Molecular , Deuterio , Escherichia coli/enzimología , Escherichia coli/genética , Marcaje Isotópico , Espectroscopía de Resonancia Magnética/métodos , Nitrógeno , Conformación Proteica , Proteínas Recombinantes/química , Proteínas Recombinantes/aislamiento & purificación , Tritio , Xilano Endo-1,3-beta-Xilosidasa , Xilosidasas/aislamiento & purificaciónAsunto(s)
Desulfovibrio vulgaris/metabolismo , Flavodoxina/química , Hidroquinonas/química , Espectroscopía de Resonancia Magnética/métodos , Oxígeno/metabolismo , Isótopos de Carbono/química , Bases de Datos como Asunto , Hidrógeno/química , Enlace de Hidrógeno , Modelos Químicos , Isótopos de Nitrógeno/química , Oxidación-Reducción , Conformación Proteica , Estructura Secundaria de Proteína , ProtonesRESUMEN
The side chain of aspartate 95 in flavodoxin from Desulfovibrio vulgaris provides the closest negative charge to N(1) of the bound FMN in the protein. Site-directed mutagenesis was used to substitute alanine, asparagine, or glutamate for this amino acid to assess the effect of this charge on the semiquinone/hydroquinone redox potential (E(1)) of the FMN cofactor. The D95A mutation shifts the E(1) redox potential positively by 16 mV, while a negative shift of 23 mV occurs in the oxidized/semiquinone midpoint redox potential (E(2)). The crystal structures of the oxidized and semiquinone forms of this mutant are similar to the corresponding states of the wild-type protein. In contrast to the wild-type protein, a further change in structure occurs in the D95A mutant in the hydroquinone form. The side chain of Y98 flips into an energetically more favorable edge-to-face interaction with the bound FMN. Analysis of the structural changes in the D95A mutant, taking into account electrostatic interactions at the FMN binding site, suggests that the pi-pi electrostatic repulsions have only a minor contribution to the very low E(1) redox potential of the FMN cofactor when bound to apoflavodoxin. Substitution of D95 with glutamate causes only a slight perturbation of the two one-electron redox potentials of the FMN cofactor. The structure of the D95E mutant reveals a large movement of the 60-loop (residues 60-64) away from the flavin in the oxidized structure. Reduction of this mutant to the hydroquinone causes the conformation of the 60-loop to revert back to that occurring in the structures of the wild-type protein. The crystal structures of the D95E mutant imply that electrostatic repulsion between a carboxylate on the side chain at position 95 and the phenol ring of Y98 prevents rotation of the Y98 side chain to a more energetically favorable conformation as occurs in the D95A mutant. Replacement of D95 with asparagine has no effect on E(2) but causes E(1) to change by 45 mV. The D95N mutant failed to crystallize. The K(d) values of the protein FMN complex in all three oxidation-reduction states differ from those of the wild-type complexes. Molecular modeling showed that the conformational energy of the protein changes with the redox state, in qualitative agreement with the observed changes in K(d), and allowed the electrostatic interactions between the FMN and the surrounding groups on the protein to be quantified.