RESUMEN
Preterm cerebral white matter injury (WMI), a major form of prenatal brain injury, may potentially be treated by oligodendrocyte (OL) precursor cell (OPC) transplantation. However, the defective differentiation of OPCs during WMI seriously hampers the clinical application of OPC transplantation. Thus, improving the ability of transplanted OPCs to differentiate is critical to OPC transplantation therapy for WMI. We established a hypoxia-ischemia-induced preterm WMI model in mice and screened the molecules affected by WMI using single-cell RNA sequencing. We revealed that endothelin (ET)-1 and endothelin receptor B (ETB) are a pair of signaling molecules responsible for the interaction between neurons and OPCs and that preterm WMI led to an increase in the number of ETB-positive OPCs and premyelinating OLs. Furthermore, the maturation of OLs was reduced by knocking out ETB but promoted by stimulating ET-1/ETB signaling. Our research reveals a new signaling module for neuron-OPC interaction and provides new insight for therapy targeting preterm WMI.
RESUMEN
OBJECTIVE: : To investigate the expression of Survivin (SVV) and its relationship with expression of p15, p16 in laryngeal squamous cell carcinoma (LSCC). METHODS: Using strep avidin-biotin complex (SABC) method, we examined the expression of SVV, p15 and p16 gene in 48 LSCC tissus samples, 24 normal laryngeal mucosa tissus samples and 24 normal laryngeal tissus adjacent to the tumors samples. RESULTS: SVV was expression in 27 of 48 (56.3%) samples of LSCC and expression in 6 of 24 (25.0%) samples of normal laryngeal tissus adjacent to the tumors. Normal laryngeal mocosa tissus samples did not expressed SVV. Overexpression of SVV was related to the tumor site, grade, clinical stage and tumor prognosis (P < 0.05). The expression of SVV in LSCC was positive correlated with p16 expression( C = 0.52 P < 0.001), but not with p15 expression. CONCLUSION: SVV may play a role in the pathway of carcinogenesis and tumor progress. It is feasible for early diagnosis and prognosis estimation. Overexpression of SVV gene and de-activation of antioncogene p16 may be play synergetic roles in the carcinogenesis of LSCC.
Asunto(s)
Carcinoma de Células Escamosas/metabolismo , Proteínas de Ciclo Celular/biosíntesis , Inhibidor p16 de la Quinasa Dependiente de Ciclina/biosíntesis , Neoplasias Laríngeas/metabolismo , Proteínas Asociadas a Microtúbulos/biosíntesis , Proteínas Supresoras de Tumor/biosíntesis , Adulto , Anciano , Anciano de 80 o más Años , Carcinoma de Células Escamosas/genética , Inhibidor p15 de las Quinasas Dependientes de la Ciclina , Femenino , Genes Supresores de Tumor , Humanos , Proteínas Inhibidoras de la Apoptosis , Neoplasias Laríngeas/genética , Masculino , Persona de Mediana Edad , Proteínas de Neoplasias , SurvivinRESUMEN
OBJECTIVE: To study the polymorphisms of DYS287 and DYS440 in Zhejiang She population. METHODS: Detection of DYS287 and DYS440 polymorphisms was performed in 100 She individuals by using polymerase chain reaction amplification and 2% agarose gel electrophoresis. RESULTS: With 150 bp product in all She individuals, YAP(+) was absent. DYS440*3 was present in 10 She individuals (10%); DYS440*4 was present in the other 90 She individuals(90%). CONCLUSION: The polymorphisms of DYS287 and DYS440 in Zhejiang She population were different from those in the other populations that belong to Sino-Tibetan Language Family. So both DYS440 and DYS287 are important and stable genetic markers, they can provide reliable evidence in human evolution study.