Increasing water nutrient reduces the availability of high-quality food resources for aquatic consumers and consequently simplifies river food webs.

While eutrophication has led to serious habitat degradation and biotic shifts in freshwater ecosystems, most current studies have focused on changes in community assemblages, with few considering the effect of eutrophication on food webs. We conducted a field study in subtropical headwater streams with a gradient of water nutrient levels to examine the effect of increasing water nutrients on food webs by using the long-chain polyunsaturated fatty acid eicosapentaenoic acid (EPA) as a measure of the nutritional quality of food. Basal food resources (macrophytes, submerged leaf litter, and periphyton), and aquatic consumers (macroinvertebrates and fish) were collected, and their fatty acid (FA) profiles were analyzed. Our results showed that periphyton was the dominant source of EPA for macroinvertebrates and fish, and a high-quality resource for consumers. As water nutrient concentrations increased, nutritional quality of periphyton significantly decreased and, in turn, the correlation between FA profiles of periphyton and macroinvertebrates declined. However, periphyton FA profiles did not account for the variability of fish FA, which may be induced by the increasing proportions of omnivorous fish in eutrophic streams that derived EPA from other sources. Further, the reduced periphyton EPA was associated with decreased trophic links and simplified stream food webs. Our study highlights the importance of high-quality food resources for aquatic food webs as water nutrients increased in stream ecosystems and provides a nutritional perspective to understand the mechanisms how eutrophication affects aquatic ecosystems.

The importance of omega-3 polyunsaturated fatty acids as high-quality food in freshwater ecosystems with implications of global change.

Traditionally, trophic ecology research on aquatic ecosystems has focused more on the quantity of dietary energy flow within food webs rather than food quality and its effects on organisms at various trophic levels. Recent studies emphasize that food quality is central to consumer growth and reproduction, and the importance of food quality for aquatic ecosystems has become increasingly well recognized. It is timely to synthesise these findings and identify potential future research directions. We conducted a systematic review of omega-3 polyunsaturated fatty acids (ω3-PUFAs) as a crucial component of high-quality food sources in freshwater ecosystems to evaluate their impact on a variety of consumers, and explore the effects of global change on these high-quality food sources and their transfer to higher trophic consumers within and across ecosystems. In freshwater ecosystems, algae rich in ω3 long-chain PUFAs, such as diatoms, dinoflagellates and cryptophytes, represent important high-quality food sources for consumers, whereas cyanobacteria, green algae, terrestrial vascular plants and macrophytes low in ω3 long-chain PUFAs are low-quality food sources. High-quality ω3-PUFA-containing food sources usually lead to increased growth and reproduction of aquatic consumers, e.g. benthic invertebrates, zooplankton and fish, and also provide ω3 long-chain PUFAs to riparian terrestrial consumers via emergent aquatic insects. Consumers feeding on high-quality ω3-PUFA-containing foods in turn represent high-quality food for their own predators. However, the ω3-PUFA content of food sources is sensitive to global environmental changes. Warming, eutrophication, increased light intensity (e.g. from loss of riparian shading), and pollutants potentially inhibit the synthesis of algal ω3-PUFAs while at the same time promoting the growth of lower-quality foods, such as cyanobacteria and green algae. These factors combined could lead to a significant reduction in the availability of ω3-PUFAs for consumers and constrain their overall fitness. Although the effect of individual environmental factors on high-quality ω3-PUFA-containing food sources has been investigated, multiple environmental factors (e.g. climate change, human activities, pollution) will act in combination and any synergistic effects on aquatic food webs remain unclear. Identifying the sources and fate of ω3-PUFAs within and across ecosystems could represent an important approach to understand the impact of multiple environmental factors on trophic relationships and the implications for populations of freshwater and riparian consumers. Maintaining the availability of high-quality ω3-PUFA-containing food sources may also be key to mitigating freshwater biodiversity loss due to global change.

Assessment of the impact of dams on aquatic food webs using stable isotopes: Current progress and future challenges.

Dams have disrupted natural river systems worldwide and although population and community level effects on aquatic biota have been well documented, food web responses remain poorly understood and difficult to characterize. The application of stable isotope analysis (SIA) provides a means to assess the effect of dams on food webs. Here we review the effect of dams on aquatic food webs using SIA, aiming to detect knowledge gaps in the field of dam impacts on aquatic food webs and propose a conceptual framework to help formulate hypotheses about dam impacts on food webs guided by food web theory. Dams can affect aquatic food webs via two pathways: a bottom-up pathway with altered basal food sources and their transfer to consumers through changes in flow, nutrients, temperature and sediment, and a top-down pathway with consumer species composition altered mainly through habitat fragmentation and related physiochemical changes. Taking these mechanisms into consideration, the impact of dams on food web attributes derived from SIA was evaluated. These studies generally apply mixing models to determine how dams alter the dominant carbon sources supporting food webs, use δ15N to examine how dams alter food-chain length, or use Layman metrics of isotope variability to assess niche changes for invertebrate and fish assemblages. Most studies compare the patterns of SIA metrics spatially (e.g. upstream vs reservoir vs downstream of dams; regulated vs unregulated rivers) and temporally (before vs after dam construction), without explicit hypotheses and/or links to theoretical concepts of food webs. We propose several steps to make SIA studies of dam impacts more rigorous and enhance their potential for producing novel insights. Future studies should quantify the shape and strength of the effect of dams on SIA-measured food web response, be conducted at larger temporal and spatial scales (particularly along the river longitudinal continuum and the lateral connected ecosystems (e.g., floodplains)), and consider effects of dams on food web resilience and tipping points.