RESUMEN
Selenium is an essential antioxidative micronutrient. This study was conducted to characterize the arsenic toxicity induced on the African fig fly, Zaprionus indianus, and its possible amelioration by selenium. We used computational tools and in vivo experiments to elucidate the mechanism of action of arsenic and selenium on Z. indianus larvae. We conducted experiments to study neurobehavioral parameters including learning and memory ability test and crawling and contraction assays. Our in silico study revealed twelve primary targets of arsenic trioxide. The gene ontology annotation of primary and secondary targets of arsenic trioxide revealed selenocysteine metabolic processes as one of the most reliable targets. To validate our in silico data, we analyzed the effect of arsenic trioxide on larvae of Z. indianus and tested the possible amelioration by sodium selenite supplementation. Our data demonstrated that the arsenic trioxide deteriorated the learning and memory ability of 2nd instar larvae of Z. indianus and such effect was reversed by sodium selenite supplementation. Furthermore, crawling and contraction assay done on 3rd instar larvae showed that there was reduction in both parameters upon arsenic trioxide exposure, which was restored with sodium selenite supplementation. Altogether, our computational and in vivo results strongly indicated that the neurobehavioral defects induced by arsenic trioxide on the larvae of Z. indianus can be successfully alleviated in the presence of sodium selenite.
Asunto(s)
Arsénico , Drosophilidae , Selenio , Animales , Larva , Trióxido de Arsénico , Selenito de Sodio , Drosophilidae/genéticaRESUMEN
BACKGROUND: The Pavo cristatus population, native to the Indian subcontinent, is thriving well in India. However, the Pavo muticus population, native to the tropical forests of Southeast Asia, has reduced drastically and has been categorised as an endangered group. To understand the probable genetic factors associated with the decline of P. muticus, we compared the mitogenome-encoded proteins (13 proteins) between these two species. RESULTS: Our data revealed that the most frequent variant between these two species was mtND1, which had an alteration in 9.57% residues, followed by mtND5 and mtATP6. We extended our study on the rest of the proteins and observed that cytochrome c oxidase subunits 1, 2, and 3 do not have any change. The 3-dimensional structure of all 13 proteins was modeled using the Phyre2 programme. Our data show that most of the proteins are alpha helical, and the variations observed in P. muticus reside on the surface of the respective proteins. The effect of variation on protein function was also predicted, and our results show that amino acid substitution in mtND1 at 14 sites could be deleterious. Similarly, destabilising changes were observed in mtND1, 2, 3, 4, 5, and 6 and mtATP6-8 due to amino acid substitution in P. muticus. Furthermore, protein disorder scores were considerably altered in mtND1, 2, and 5 of P. muticus. CONCLUSIONS: The results presented here strongly suggest that variations in mitogenome-encoded proteins of P. cristatus and P. muticus may alter their structure and functions. Subsequently, these variations could alter energy production and may correlate with the decline in the population of P. muticus.
RESUMEN
Accumulation of Fluoride (F) was found in the soil and vegetation of the F-endemic villages of Gaya district, Bihar, India. The mean F level in the groundwater of F non-endemic (control) area was 0.59 ± 0.03 (n = 11), while that of F-endemic area was 2.36 ± 0.23 (n = 27). Water soluble F (WSF) and total F (TF) in the soil of F-endemic villages were significantly higher as compared to the F non-endemic area. Similarly, WSF and TF in the vegetables and the grain crops (cereals, legumes and oilseeds) of the F-endemic area were significantly higher as compared that of the control area. Leafy vegetables showed higher accumulation of F with WSF and TF in spinach ranging from 3.62 to 4.82 and 9.88-12.88 mg/kg respectively. The WSF and TF in coriander ranged from 9.66 to 10.88 and 23.11-25.73 mg/kg respectively.
Asunto(s)
Flúor/química , Flúor/metabolismo , Contaminantes del Suelo/química , Suelo/química , Verduras/química , Grano Comestible , Análisis de los Alimentos , Agua Subterránea/química , Humanos , India , Contaminantes del Suelo/metabolismoRESUMEN
Groundwater used for drinking and cooking was analysed for fluoride (F), and health surveys were conducted in Bodh Gaya, Amas and Bankebazaar blocks of the Gaya district, Bihar, India. Amas and Bankebazaar blocks were F endemic areas with mean F = 2.36 ± 0.23 mg/L (N = 27). Bodh Gaya was considered as control area with mean F = 0.59 ± 0.03 mg/L (N = 11). Health survey showed that more than 50 % of adults and more than 55 % of children had complaints of gastro-intestinal (GI) disturbances in the F endemic areas, while less than 20 % of adults and less than 10 % of children complained of GI problems in the control areas. Haematological analyses were conducted on age- and sex-matched fluorotic subjects (N = 93) of F endemic areas, and non-fluorotic subjects (N = 52) of control area showed lowered haemoglobin, haematocrit, mean corpuscular volume, mean corpuscular haemoglobin, and mean corpuscular haemoglobin concentration in the fluorotic subjects, suggesting the occurrence of anaemia in the fluorotic subjects.
Asunto(s)
Fluoruros/toxicidad , Enfermedades Gastrointestinales/sangre , Pruebas Hematológicas , Adulto , Estudios de Casos y Controles , Niño , Agua Potable/química , Enfermedades Endémicas , Fluoruros/análisis , Enfermedades Gastrointestinales/epidemiología , Encuestas Epidemiológicas , Humanos , India/epidemiologíaRESUMEN
We studied the health problems of 197 female beedi rollers in Patna, Bihar, India to ascertain the effects of beedi rolling on health. The study found that more than 70% of the beedi rollers suffered from eye, gastrointestinal and nervous problems while more than 50% of the respondents suffered from respiratory problems, mostly throat burning and cough. More than 75% of the respondents faced osteological problems. Total RBC, WBC and platelet counts of the beedi rollers were significantly lower in comparison to the control subjects. Differential leucocyte count showed significantly risen lymphocytes and eosinophils and lowered neutrophils and monocytes in the beedi rollers as compared to the control group. Haemoglobin levels were lower among beedi rollers compared to the control group. SGPT (ALT) enzyme concentration, a parameter of liver dysfunction was significantly higher in the beedi rollers as compared to the control group. Thus, the study concluded that beedi rolling may cause significant health hazards.
Asunto(s)
Sustancias Peligrosas/toxicidad , Nicotiana , Enfermedades Profesionales/epidemiología , Enfermedades Profesionales/etiología , Exposición Profesional/efectos adversos , Adulto , Alanina Transaminasa/metabolismo , Aspartato Aminotransferasas/metabolismo , Estudios de Casos y Controles , Enfermedades del Sistema Nervioso Central/inducido químicamente , Enfermedades del Sistema Nervioso Central/epidemiología , Enfermedades del Sistema Nervioso Central/etiología , Oftalmopatías/inducido químicamente , Oftalmopatías/epidemiología , Oftalmopatías/etiología , Femenino , Enfermedades Gastrointestinales/inducido químicamente , Enfermedades Gastrointestinales/epidemiología , Enfermedades Gastrointestinales/etiología , Hemoglobinas/metabolismo , Humanos , India/epidemiología , Leucocitos/citología , Leucocitos/efectos de los fármacos , Leucocitos/metabolismo , Hepatopatías/epidemiología , Hepatopatías/etiología , Persona de Mediana Edad , Enfermedades Profesionales/inducido químicamente , Enfermedades Profesionales/fisiopatología , Salud Laboral , Factores Socioeconómicos , Nicotiana/química , Nicotiana/toxicidad , Adulto JovenRESUMEN
We investigated the effects of three different pesticides (carbendazim, dimethoate, and glyphosate) and their mixture on the growth and reproduction of the earthworm species, Eisenia fetida. The study was conducted following the suggestion of the International Workshop on Earthworm Ecotoxicology. The results showed that the pesticide treatment had a marked negative impact on the growth and reproduction of earthworms. Carbendazim and dimethoate were found to cause greater harm to the selected earthworm species than glyphosate.