1.
Chemistry
; 28(70): e202202397, 2022 Dec 15.
Artículo
en Inglés
| MEDLINE
| ID: mdl-36082977
RESUMEN
Phomoxanthone A is a naturally occurring molecule and a powerful anti-cancer agent, although its mechanism of action is unknown. To facilitate the determination of its biological target(s), we used affinity-based labelling using a phomoxanthone A probe. Labelled proteins were pulled down, subjected to chemoproteomics analysis using LC-MS/MS and ATP synthase was identified as a likely target. Mitochondrial ATP synthase was validated in cultured cells lysates and in live intact cells. Our studies show sixty percent inhibition of ATP synthase by 260â µM phomoxanthoneâ A.