Evaluation of total oxidative stress parameters in patients with nasal polyps.

Oxidative stress, an imbalance between reactive oxygen species production and antioxidative defense activity, is believed to have a role in the development and pathogenesis of nasal polyps (NPs). Based on this assumption, several known oxidants and antioxidants have been studied in patients with NPs. The purpose of this study was to evaluate the association between oxidative stress parameters with a more valid and reliable method in patients with NPs. Seventy-three patients with NPs, septal deviations and middle concha hypertrophies were recruited. Patients were divided into two groups; group 1 (n = 38) consisted of patients with NPs, and group 2 (n = 35) included patients with septal deviations and middle concha hypertrophies. Polyp specimens were taken from all patients who underwent endoscopic surgery for NPs. Control specimens were obtained from patients who underwent an operation for septoplasty or middle concha hypertrophy. Blood and tissue samples were obtained to assess total oxidant status (TOS), total antioxidant status (TAS) and oxidative stress index (OSI). Compared to group 2, group 1 had significantly higher TOS and OSI and lower TAS levels both in serum and tissue samples (p < 0.001 for all). In group 1, tissue TOS and OSI levels were significantly higher, and TAS levels were significantly lower than in serum (p < 0.001 for all), whereas no significant difference was found in TOS, OSI and TAS levels either in serum or tissue samples in group 2 (p = 1.0; p = 1; p = 0.208, respectively). In group 1, serum OSI levels were significantly correlated with age (r = 0.442, p = 0.005). Our study demonstrated that oxidative stress, both in serum and tissues in patients with NPs, was higher than in patients without NPs. Our study differs from previous studies in that we used a more reliable method that measures both TOS and TAS.

Prolidase activity and oxidative stress parameters in patients with nasal polyps.

BACKGROUND AND AIM: Oxidative stress is believed to have a role in the development of nasal polyps (NPs). It is also known that prolidase activity increases secondary to chronic inflammation. The purpose of this study was to evaluate the association between prolidase activity and oxidative stress parameters in patients with NPs. MATERIALS AND METHODS: Sixty patients with NPs, septal deviations and the concha hypertrophies were recruited to the study. Patients were divided into two groups; group 1 (n=30) consisted of patients with NPs, and group 2 (n=30) included patients with septal deviations and concha hypertrophies. Polyp specimens were taken from all patients who underwent endoscopic surgery for NPs. Control specimens were acquired from patients who underwent an operation for septoplasty or concha hypertrophy. Blood and tissue samples were obtained to assess lipid hydroperoxide (LOOH), catalase (CAT) and prolidase activity. RESULTS: Compared to group 2, group 1 had significantly higher LOOH and prolidase levels, and lower CAT levels both in serum and tissue samples (p<0.05 for all). Prolidase activity was correlated with increased LOOH and decreased CAT levels (r = 0.507 p = 0.004; r = - 0.579, p = 0.001, respectively). CONCLUSIONS: Our study demonstrated that oxidative stress and prolidase activity, both in serum and the tissue in patients with NPs, were higher than in patients without NPs.

Oxidative stress index may play a key role in patients with pemphigus vulgaris.

BACKGROUND: Increased reactive oxygen species (ROS) and lipid peroxidation are seen in many dermatologic disorders, including atopic dermatitis, psoriasis, vitiligo, acne vulgaris, pemphigus vulgaris (PV), lichen planus and alopecia areata. In PV, the increased production of ROS from activated neutrophils reduces the concentrations of antioxidant vitamins and enzymes. OBJECTIVE: The present study aims to determine the oxidative stress index (OSI) by studying serum total oxidant capacity (TOC), lipid hydroperoxide (LOOH) and total antioxidant capacity (TAC) in PV patients. METHODS: The study included 27 PV patients and a control group consisting of 24 healthy volunteers. Serum TOC, LOOH and TAC levels were examined and OSI was measured in the PV patients and the control group. RESULTS: TOC (P = 0.001) and LOOH (P = 0.001) levels as well as OSI (P = 0.001) were found higher in the PV patients, relative to the control group. Serum TAS (P = 0.221) did not differ between groups. Serum TOC, LOOH and TAC levels and OSI in PV patients with mucosal involvement were not different than those in mucocutaneous PV patients. CONCLUSION: Serum TOC and LOOH levels and OSI were higher in the PV patients, in comparison with the control group. Serum TAC level was not different.