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BACKGROUND: Inflammation occurring after vascular endothelial damage plays a role in thrombus formation. Changes in various blood parameters that develop after the inflammatory condition can be used as a marker to predict thrombus. AIM: This study aimed to investigate the relationship between the neutrophil/lymphocyte ratio (NLR), platelet/lymphocyte ratio (PLR), and portal vein thrombosis (PVT). MATERIALS AND METHODS: After applying the exclusion and inclusion criteria to the patients diagnosed with PVT and followed up in our center between January 2006 and May 2018, a total of 38 patients without acquired risk factors for the development of PVT and 52 healthy controls were included in the study. Clinical features and NLR and PLR at diagnosis were evaluated. RESULTS: NLR and PLR values were detected to be significantly higher in patients diagnosed with PVT compared to the control group (P < 0.001 for NLR, P < 0.001 for PLR). Findings were as follows: In acute PVT patients for NLR = 3.645 (area under the receiver operating characteristic (AUROC) 0.886, sensitivity 69.2%, specificity 96.2%, P < 0.001), for PLR = 196.24 (AUROC 0.754, sensitivity 53.2%, specificity 96.2%, P = 0.005), while in chronic PVT patients, for NLR = 3.645 (AUROC 0.744, sensitivity 40%, specificity 96.2%, P = 0.001), and for PLR = 195.93 (AUROC 0.715, sensitivity 44%, specificity 96.2%, P = 0.002). CONCLUSION: NLR and PLR were associated with the diagnosis of PVT. In PVT patients, NLR and PLR values were observed to be significantly higher than the control group. In our study, the relationship between NLR and PLR in patients with noncirrhotic, nonmalignant PVT without acquired risk factors for thrombosis was shown for the first time.
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Trombosis , Trombosis de la Vena , Humanos , Neutrófilos/patología , Vena Porta , Recuento de Plaquetas , Linfocitos/patología , Plaquetas/patología , Trombosis de la Vena/complicaciones , Factores de Riesgo , Estudios RetrospectivosRESUMEN
Background: During physiological root resorption of deciduous teeth, apoptotic cell death triggered by physiological processes might play a role in physiological root resorption in addition to collagen destruction. Little information has been obtained about the sequence of events and the mechanism responsible for the physiological death of pulp tissue cells. Aim: This study evaluated apoptotic cell death in the pulp tissue of deciduous teeth that showed various levels of physiological root resorption. The role of apoptosis in pulp tissue elimination during the physiological resorption of deciduous teeth was also examined. Materials and Methods: For orthodontic reasons, 12 healthy permanent teeth and the pulp of 34 healthy deciduous teeth showing signs of early and advanced root resorption were extracted. To detect apoptotic cells in the pulp tissue, the terminal deoxynucleotidyl transferase dUTP nick end-labeling (TUNEL) method and transmission electron microscopy (TEM) were used. The apoptotic index (AI) values of the study groups were determined using the TUNEL method. This technique required calculating the Apoptag positive(+) fibroblast cell ratio in accordance with the total number of cells. Results: No statistically significant differences were found for the AI values of each study group (p>0.05). Apoptosis was detected in the vascular endothelial cells, the mononuclear inflammatory cells, and the odontoblasts of the connective pulp tissue. In the pulp tissue, evaluated using TEM, various pulp cells were observed at distinct stages of apoptosis. Conclusion: The similarity between the AI values for both study groups suggested that in early and advanced stages of resorption, apoptosis may contribute to the regulation of the pulp cell population in a way that does not relate to the physiological process of deciduous teeth root resorption.
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Resorción Radicular , Humanos , Células Endoteliales , Apoptosis , Leucocitos , Diente PrimarioRESUMEN
The in vitro study demonstrates wirelessly controlled modulation of neural activity using magnetoelectric nanoparticles (MENPs), synchronized to magnetic field application with a sub-25-msec temporal response. Herein, MENPs are sub-30-nm CoFe2O4@BaTiO3 core-shell nanostructures. MENPs were added to E18 rat hippocampal cell cultures (0.5 µg of MENPs per 100,000 neurons) tagged with fluorescent Ca2+ sensitive indicator cal520. MENPs were shown to wirelessly induce calcium transients which were synchronized with application of 1200-Oe bipolar 25-msec magnetic pulses at a rate of 20 pulses/sec. The observed calcium transients were similar, in shape and magnitude, to those generated through the control electric field stimulation with a 50-µA current, and they were inhibited by the sodium channel blocker tetrodotoxin. The observed MENP-based magnetic excitation of neural activity is in agreement with the non-linear M - H hysteresis loop of the MENPs, wherein the MENPs' coercivity value sets the threshold for the externally applied magnetic field.
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Calcio , Nanopartículas , Ratas , Animales , Campos Magnéticos , Nanopartículas/química , Neuronas/fisiologíaRESUMEN
PURPOSE: To investigate colour agreement between different shades of Panavia V5 resin cement and their try-in pastes under zirconia-reinforced lithium silicate ceramics. METHODS: Forty-four zirconia-reinforced lithium silicate ceramic specimens were prepared at 0.8 ± 0.01 mm thickness. Composite resin tooth-shaded background specimens were obtained in two shades. Resin cement specimens were fabricated at 0.1-mm thickness. Colour difference values (ΔE) between Aquagel and both the try-in paste and resin cement were calculated using the CIE-Lab system. The CIEDE2000 (ΔE00) colour formula was used to determine colour differences. The three-way ANOVA test and a multiple comparisons test were used for statistical analysis (α = 0.05). RESULTS: A statistically significant difference was found between the ΔE00 values obtained from the cement shade colour measurements for all specimens (P < 0.05). According to the three-way ANOVA test, lower ΔE00 values were observed in the try-in paste group compared to the resin cement group. The ΔE00 values were significantly affected by the cement shade and tooth-shaded background (P < 0.05). CONCLUSIONS: The colour agreement between the try-in pastes and the corresponding resin cement differed, and the try-in pastes could not mask the dark dental background as much as the corresponding resin cement.
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Porcelana Dental , Cementos de Resina , Cerámica , Color , Humanos , Litio , Ensayo de Materiales , Silicatos , Propiedades de Superficie , CirconioRESUMEN
OBJECTIVE: The lymphocyte-to-C-reactive protein ratio (LCRP) and Systemic Immune-Inflammation Index (SII) can successfully predict 28-day mortality rates with community-acquired pneumoniaMETHODS: This prospective study was conducted in 2018. Hospitalized patients underwent follow-up evaluations 28 days after admission. RESULTS: A total of 345 patients with CAP were enrolled in this study. All-cause mortality at the 28th day of follow-up was 13.6 %. There were statistically significant results between the 2 groups (survivors and non-survivors), in terms of the LCRP, SII, PSI, and CURB-65 values. Moreover, the optimal LCRP cutoff for predicting 28-day mortality was determined to be 4, with 89 % sensitivity, 73 % specificity. Based on the average SII>3551for predicting 28-day mortality, the sensitivity, specificity was 63.8 %, 68.1 % respectively. When the value of the cutoff PSI was ≥130 points, the sensitivity, specificity was 68 %, 65 %, respectively. Based on 3 points and above as the cutoff value of the CURB-65 score, the sensitivity, specificity was 80 %, 68 %, respectively. ROC curve analysis revealed that the areas of LCRP, SII, PSI, and CURB-65 under the AUC in terms of 28-day mortality were 0,820,0,737,681, and 0,773, respectively,CONCLUSIONS: LCRP and SII level are valuable for predicting the mortality rate among patients with CAP at ED admission (Tab. 3, Fig. 3, Ref. 27).
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Infecciones Comunitarias Adquiridas , Neumonía , Biomarcadores , Infecciones Comunitarias Adquiridas/diagnóstico , Humanos , Inflamación , Neumonía/diagnóstico , Pronóstico , Estudios Prospectivos , Índice de Severidad de la EnfermedadRESUMEN
Pentraxin 3 (PTX3), a prototypical member of the long pentraxin subfamily, is a evolutionarily conserved multimeric pattern recognition receptor involved in the humoral component of the innate immune system. Pentraxin 3 is released when tissue is stressed or damaged, and interacts with many different ligands. Pentraxin 3 exerts a pivotal role both as a regulator and as an indicator of inflammatory response in the pathogenesis of many diseases such as sepsis, vasculitis and preeclampsia. Uncontrolled inflammatory response is considered a major cause of unexplained recurrent pregnancy loss (URPL). We determined the PTX3 messenger ribonucleic acid (mRNA) and protein expression levels in placentai tissues from 50 women with URPL, and made comparison with those in 50 age-matched control subjects. In quantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemistry analyses, PTX3 mRNA and protein levels, respectively, were significantly increased in URPL patients compared with their respective controls (p = 0.0001). Although no significant correlations were identified between PTX3 expression levels and clinical parameters such as maternal age, numbers of previous pregnancy losses, and gestational age at miscarriage, PTX3 mRNA expression was significantly higher in patients with no live births than in women with previous live births (p = 0.0001). Our study suggests that tissue-specific expression of PTX3 is associated with URPL. Further larger studies are required to determine whether PTX3 expression can be used as a biomarker to manage URPL in routine clinical practice.
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Twenty eight male Sprague Dawley rats (aged 3 months) were used in the study. The animals were given feed and water as ad libitum. Sprague dawley rats were randomly divided into 4 groups as 7 rats in each group. Except for the control one, aflatoxin B1 (7.5 µg / 200 g), resveratrol (60 mg / kg) was administered to rats of 3 other groups. At the end of the 16th day, blood, semen and tissue specimens were taken by decapitation under ether anesthesia. When we evaluate the spermatological parameters, it is understood that resveratrol has a statistically significant difference in terms of sperm motility and viability (membrane integrity) compared to the control group and aflatoxin B1 administration groups, indicating a protective effect on spermatological parameters. In terms of pathological parameters - histopathological examination - in the control and resveratrol groups, seminiferous tubules were observed to be in normal structure. In the group treated with aflatoxin, the regular structure of the spermatogenic cells deteriorated and the seminiferous tubules became necrotic and degenerative. In the group treated with Afb1 + res, the decreasing of necrotic and degenerative changes were determined compared with in the group treated with aflatoxin. As immunohistochemical examination, cleaved caspase 3 expression was found to be very low in the control and resveratrol groups. Cleaved caspase 3 expression was severely exacerbated in seminiferous tubules in aflatoxin group but cleaved caspase 3 expression level decreased in Afb1 + res. In the biochemical direction, resveratrol has been shown to inhibit the adverse effects of aflatoxin on antioxidant levels and to show a protective effect. For this purpose, the use of resveratrol with antioxidant activity was investigated in preventing or ameliorating damage to aflatoxin B1. It has been concluded that resveratrol effectively prevent the aflatoxin-induced testicular damage and lipid peroxidation. It has also been shown that resveratrol has protective effects on sperm motility and viability.
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Aflatoxina B1/toxicidad , Resveratrol/farmacología , Testículo/efectos de los fármacos , Animales , Antioxidantes/administración & dosificación , Antioxidantes/farmacología , Catalasa/genética , Catalasa/metabolismo , Regulación Enzimológica de la Expresión Génica/efectos de los fármacos , Glutatión/metabolismo , Glutatión Peroxidasa/genética , Glutatión Peroxidasa/metabolismo , Inmunohistoquímica , Masculino , Malondialdehído/metabolismo , Ratas , Ratas Sprague-Dawley , Espermatogénesis/efectos de los fármacos , Superóxido Dismutasa/genética , Superóxido Dismutasa/metabolismo , Testículo/patologíaRESUMEN
Standardized clinical diagnostic procedures cannot assess the functionality of the anatomical structures in sport-specific movement. Biomechanical screening is able to detect deficits but is not sufficiently and objectively precise with the current clinical examination tools including conventional imaging techniques. The fields of use of functional testing methods are versatile and range from injury prevention analysis, screening during rehabilitation phases up to the return-to-play decision. Using simple musculoskeletal function analysis it is difficult to assess the risk of injuries. The main advantage of instrumented 3D-motion analysis is its potential to generate objective, reliable and reproducible data with exact joint angles, muscle activity, as well as loading inside the joints during movement. These marker-based motion analysis procedures are more time-consuming and more cost intensive and necessitate in particular biomechanical and medical knowledge to assess the analytical data in terms of clinical relevance. In the absence of scientific studies on biomechanical analyses in professional sports, this study shows preliminary approaches to this topic.
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Traumatismos en Atletas , Traumatismos de la Rodilla , Deportes , Fenómenos Biomecánicos , Humanos , MovimientoRESUMEN
BACKGROUND AND AIMS: Hepatitis B virus (HBV) infection is a global threat and with the growing cultural diversity in Western Europe, knowledge on routes of infection in order to decrease HBV spreading is essential. This study assessed the risk of horizontal transmission through non-sexual close contact in the chronic hepatitis B (CHB) population in Maastricht (the Netherlands) and Genk (Belgium), with a main focus on the differences between ethnic groups. METHODS: In this multicenter retrospective study, 166 CHB patients, who were still under follow-up between December 2009 to December 2014, were recruited from the Hepatology Outpatient Departments of two hospitals, one in Maastricht and one in Genk. Ethnicity (defined as country of origin (COO)) and routes of transmission were collected from all patients. RESULTS: The CHB population in Maastricht and Genk consisted of 98 and 68 patients, respectively. In Maastricht, 31% were of Dutch and 16% of Chinese origin. In Genk, mainly Belgian (15%) and Turkish (50%) patients were included. The percentage of horizontal transmission in the total study cohort was 9%. Moreover, the COO groups Dutch/Belgian (n=40), Turkish (n=38) and Chinese (n=18) differed in the number of cases infected by horizontal transmission (4%, 30% and 6%, p=0.030). CONCLUSION: Although the prevalence of horizontal transmission in the total study cohort is low, non-sexual close contact may play a role in the migrant population, particularly the Turkish. This should be an important public health target with respect to the prevention of HBV spreading.
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Virus de la Hepatitis B/aislamiento & purificación , Hepatitis B Crónica/transmisión , Bélgica/epidemiología , Hepatitis B , Hepatitis B Crónica/diagnóstico , Hepatitis B Crónica/epidemiología , Humanos , Estudios Retrospectivos , Turquía/etnologíaRESUMEN
The aim of this study was to determine the prognostic role of echocardiography and compare with admission N-terminal proB-type natriuretic peptide (NT-proBNP) levels in adult patients with community-acquired pneumonia (CAP). Consecutive adult patients hospitalized with CAP were prospectively enrolled and followed-up until hospital discharge or death. Echocardiography was performed within the first 48 hours. Complicated hospitalization (CH) was defined as intensive care unit admission, need for mechanical ventilation or in-hospital mortality. This study was registered with ClinicalTrials.gov, number NCT02441855. A total of 15 CH (13.5%) occurred among 111 patients with CAP. CAP patients with a CH compared with those without CH had significantly higher NT-proBNP values (1267.4±1146.1 vs. 305.6±545.7 pg/mL, p <0.001) and troponin I (23.8±24.3 vs. 10.3±6.3 ng/mL, p 0.02) but had lower left ventricle ejection fraction (52.7±8.7 vs. 60.5±6.7%, p <0.001) and tricuspid annular plane systolic excursion (TAPSE), which is a measure of right ventricular systolic function (17.1±4.4 vs. 21.8±4 mm; p 0.001). Patients with elevation of NT-proBNP and decreased TAPSE at presentation had a significantly higher probability of CH (60%) than patients with either elevated NT-proBNP or decreased TAPSE (40%). Patients with neither elevated NT-proBNP nor decreased TAPSE had a 0% probability of CH. This is the first study to demonstrate that decreased right ventricular systolic function is associated with increased rates of adverse events in patients with CAP.
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Biomarcadores/sangre , Infecciones Comunitarias Adquiridas/sangre , Ecocardiografía , Cardiopatías/sangre , Neumonía/sangre , Anciano , Antibacterianos/uso terapéutico , Infecciones Comunitarias Adquiridas/complicaciones , Infecciones Comunitarias Adquiridas/tratamiento farmacológico , Determinación de Punto Final , Femenino , Estudios de Seguimiento , Cardiopatías/diagnóstico , Cardiopatías/etiología , Mortalidad Hospitalaria , Humanos , Masculino , Persona de Mediana Edad , Péptido Natriurético Encefálico/sangre , Fragmentos de Péptidos/sangre , Neumonía/complicaciones , Neumonía/tratamiento farmacológico , Pronóstico , Estudios ProspectivosRESUMEN
INTRODUCTION: A rule exists regarding the use of computed tomography (CT) for patients presenting to the emergency department with head trauma and a Glasgow coma score (GCS) of 15; however, it can be difficult to make this decision due to overcrowded emergency rooms or exaggerated patients complaints. We evaluated patients who presented to the emergency room with minor head trauma, and we aimed to investigate the relationship between brain pathology on CT and hematological markers in order to find markers that help us identify brain pathology in patients with a GCS of 15. METHODS: This retrospective study included 100 patients with pathologies present on their CT scans and a control group consisting of 100 patients with a normal CT. All data obtained from this study were recorded and evaluated using "Statistical Package for Social Sciences for Windows 20" program. Parametric tests (independent samples test) were used with normally distributed data, while non-parametric tests (Mann-Whitney U test) were used with non-normally distributed data. A p ≤ 0.05 was considered significant. FINDING: When we divided the cases into two groups based on the presence of pathologies on CT scan, we determined that there were significant differences between the groups in terms of white blood cell (WBC), hemoglobin (Hb), mean platelet volume (MPV), neutrophil (neu), troponin T, neutrophil/lymphocyte ratio (NLR) and platelet/lymphocyte ratio (PLR). When differentiating the patients that had brain pathologies on CT scan from patients that had normal CT scans, the troponin T cut-off value of 6.16 lead to 90 % specificity, and setting the NLR cut-off value at 4.29 resulted in a specificity of 90 %. CONCLUSION: MPV, NLR, and troponin T can be used as parameters that indicate brain pathologies on CT scans of patients presenting to the emergency department with isolated minor head trauma and GCS of 15 when the necessity of a CT scan is otherwise unclear.
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Traumatismos Craneocerebrales/sangre , Servicio de Urgencia en Hospital , Inflamación/sangre , Adulto , Biomarcadores/sangre , Traumatismos Craneocerebrales/diagnóstico por imagen , Toma de Decisiones , Femenino , Escala de Coma de Glasgow , Humanos , Puntaje de Gravedad del Traumatismo , Masculino , Valor Predictivo de las Pruebas , Estudios Retrospectivos , Tomografía Computarizada por Rayos XRESUMEN
BACKGROUND: The aim of this study was to investigate the disease characteristics of children with juvenile idiopathic arthritis (JIA) in southeast Turkey. METHODS: The International League of Associations for Rheumatology (ILAR) criteria were used to diagnose JIA. Hospital records of the Pediatric Rheumatology Unit, of the Dicle University Hospital, were reviewed retrospectively and demographic, clinical and laboratory data were recorded. RESULTS: Totally 213 children (103 boys, 110 girls), with an age range of 1.6-18 years were enrolled. The mean age of the disease onset was 8.1 years. Polyarticular type was the most common (42.3%) presentation. The frequencies of other JIA subtypes were as follows: oligoarticular 37.1%, systemic 8.9%, enthesitis-related arthritis (ERA) 10.8% and psoriatic arthritis 0.9%. The knees (74.2%) and ankles (54.0%) were the most commonly affected joints. Uveitis was found in 4.2% of patients. Anti-nuclear antibodies were positive in 11.7% and HLA-B27 in 2.8% of patients. Active disease was seen in 57 (26.7%) patients at the last visit. CONCLUSION: In the present study, polyarticular JIA was the predominant subtype and there were fewer patients with positive ANA or uveitis compared to previous studies. Hippokratia 2015, 19 (1): 63-68.
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OBJECTIVE: To identify the level of depression, the level of life quality, and the relationship between these, in patients applying to sleep centers for various sleep problems. METHODS: This cross-sectional study included 229 patients who applied for polysomnography at sleeping centers under supervision of the Neurology and Chest Diseases Clinics of Kayseri Education and Research Hospital, Kayseri, Turkey between June and August 2013. The data collection tools were a socio-demographical data form, Beck Depression Inventory (BDI), Pittsburgh Sleep Quality Index (PSQI), and the World Health Organization Quality of Life Scale (WHOQOL-BREF). For statistical analyses, the Student t-test, Kruskal-Wallis-variant analysis, and chi-square tests were used. Significance level was considered as p<0.05. RESULTS: In our study, patients who were older aged, married, not working, and who had a chronic disease, and a severe depressive symptom were observed to have significantly poorer sleep quality. While patients with any chronic disease had significantly higher scores for total PSQI and depression, their physical, mental, and social WHOQOL-BREF scores were significantly lower. The PSQI total scores, and depression scores of the smoking patients were significantly higher for physical, mental, and social WHOQOL-BREF fields. There was a positive correlation between PSQI scores and BDI scores while there was a negative correlation among BDI, PSQI, and WHOQOL-BREF life quality sub-scale scores. CONCLUSION: Sleep quality was significantly poorer in patients who were older aged, married, not working, and who had a chronic disease, and a severe depressive symptom. There was a significantly negative correlation among depression, sleep quality, and life quality, while there was a significantly positive correlation between life quality and depression.
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Depresión/epidemiología , Calidad de Vida , Trastornos del Sueño-Vigilia/epidemiología , Adolescente , Adulto , Anciano , Femenino , Humanos , Masculino , Persona de Mediana Edad , Índice de Severidad de la Enfermedad , Factores Socioeconómicos , TurquíaRESUMEN
BACKGROUND: Results are conflicting with respect to the renal effects of anti-viral agents used for hepatitis B virus infection. AIM: To compare short and long-term renal effects in real-life settings and to determine risk factors for renal impairment during treatment. METHODS: 2221 treatment-naïve patients were enrolled. Among these, 895 (302 lamivudine, 27 telbivudine, 282 entecavir, 273 tenofovir and 11 adefovir initiated patients) had 'repeated measures' of creatinine (baseline, 1st, 6th, 12th and 24th month of treatment). Telbivudine and adefovir groups were excluded from further analysis because of the low number of patients. We calculated the glomerular filtration rate (GFR) using the Modification of Diet in Renal Disease (MDRD) formula at each time point. Hypophosphataemia was also recorded. Risk factors for renal impairment were analysed. RESULTS: Tenofovir caused a decline in GFR at each time point when compared to baseline levels. However, lamivudine and entecavir did not change GFR. GFR-shifting from ≥90 to 60-89 mL/min/1.73 m(2) was comparable among groups. The proportion of patients whose baseline creatinine increased more than 25% was comparable among all anti-virals. GFR showed a decline in patients who switched from entecavir to tenofovir. One patient with compensated cirrhosis needed to change from tenofovir because of renal safety. Seven and three patients developed transient hypophosphataemia in the tenofovir and lamivudine groups, respectively. CONCLUSIONS: Although tenofovir caused a decline in GFR, differences between the anti-viral agents do not appear to be so impressive. In patients with and without renal risk factors at baseline, there is no impact of anti-virals, including tenofovir.
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Antivirales/uso terapéutico , Hepatitis B Crónica/tratamiento farmacológico , Cirrosis Hepática/etiología , Insuficiencia Renal/inducido químicamente , Adulto , Antivirales/efectos adversos , Creatinina/metabolismo , Femenino , Tasa de Filtración Glomerular , Virus de la Hepatitis B/aislamiento & purificación , Humanos , Cirrosis Hepática/tratamiento farmacológico , Masculino , Persona de Mediana Edad , Insuficiencia Renal/epidemiología , RiesgoRESUMEN
AIM: The aim of this literature-based descriptive study was to examine the reporting of the observational research studies published in peer-reviewed nursing journals in Turkey. METHODS: Eleven peer-reviewed nursing journals printed on a regular basis in Turkey between 2007 and 2012 were selected. These journals were searched for observational research studies, and 502 studies were selected and examined by using the Strengthening the Reporting of Observational Studies in Epidemiology Statement. RESULTS: Of the 502 studies, 495 were cross-sectional, 3 were cohort, and 4 were case controlled. Summary and introduction and aim sections were sufficient in most of the studies. The methods sections of the reports were mostly not reported: 64.3% of the reports did not indicate eligibility/inclusion criteria; sampling method, 67.0%; possible sources of bias, 99.2%; ways to reach sample size, 92.6%. In the results section, the number of individuals participating in each stage of the studies (44.0%) and in other analyses made (39.2%) was not reported. In the discussion section, a main comment about research findings was partly made (97.4%), and limitations of the studies and possible sources of bias were not written in 99.0% of the studies. CONCLUSIONS: This study clearly revealed that the observational research studies published in nursing journals in Turkey did not fulfil the important criteria and needed to be improved. IMPLICATIONS FOR NURSING AND HEALTH POLICY: Information obtained from this study can contribute to improvement of the quality of reporting observational studies in nursing and thus using obtained findings in practice.
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Bibliometría , Investigación en Enfermería , Estudios Observacionales como Asunto/normas , Publicaciones Periódicas como Asunto , Informe de Investigación , Humanos , Proyectos de Investigación , TurquíaRESUMEN
PURPOSE: The aim of the current study was to evaluate potential relationships between serum adropin levels and metabolic parameters in polycystic ovary syndrome (PCOS). MATERIALS AND METHODS: Twenty women with PCOS and 20 healthy, age and body mass index (BMI) matched controls were included in the study. All subjects underwent venous blood drawing on the early follicular phase after an overnight fasting. Serum adropin levels were measured with enzyme immunosorbent assay (EIA). The relationships between serum adropin levels and metabolic parameters were also assessed. RESULTS: Serum adropin levels were found to be significantly lower in women with PCOS when compared to control group (p < 0.001). Serum adropin level was correlated negatively with fasting serum insulin levels, homeostasis model of assessment - insulin resistance (HOMA-IR) and serum lipid markers including cholesterol, very low-density lipoprotein, and triglycerides (TG) in PCOS patients (p < 0.05). CONCLUSION: The findings of current study suggest that women with PCOS have low serum adropin levels that may contribute to the underlying pathogenesis of metabolic disturbances in PCOS.
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Proteínas Sanguíneas/metabolismo , Síndrome del Ovario Poliquístico/sangre , Adolescente , Adulto , Biomarcadores/sangre , Estudios de Casos y Controles , Colesterol/sangre , Ayuno/sangre , Femenino , Hormona Folículo Estimulante/sangre , Fase Folicular/sangre , Homeostasis , Humanos , Insulina/sangre , Resistencia a la Insulina , Péptidos y Proteínas de Señalización Intercelular , Lipoproteínas VLDL/sangre , Hormona Luteinizante/sangre , Péptidos , Triglicéridos/sangre , Adulto JovenRESUMEN
The goal of the present clinical study was to evaluate new bone formation in human extraction sockets augmented with enamel matrix derivatives (EMD) and Bio-Oss Collagen. Patients with symmetrical single-rooted teeth in the bilateral quadrants of the upper jaw condemned for extraction participated in this study. Following extraction, the sockets (20 sockets) were randomly augmented using either EMD or Bio-Oss Collagen. After 3 months of healing, bone biopsies were obtained and prepared for histological analyses. Dental implants were then placed. Implant stability quotient (ISQ) readings were obtained for each implant at the time of surgery and at 1 and 3 months postoperatively. The mean new bone formation was 34.57 ± 25.67% in the EMD sites and 28.80 ± 16.14% in the Bio-Oss Collagen sites. There was no significant difference between the groups. The ISQ values were significantly higher for the implants placed in the EMD sites at the first and third months, but no significant differences were observed in the ISQ values for the implants placed in the Bio-Oss Collagen sites. The augmentation of the extraction sockets with EMD or Bio-Oss Collagen leads to similar behaviour in bone regeneration.
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Proceso Alveolar/citología , Aumento de la Cresta Alveolar/métodos , Regeneración Ósea/efectos de los fármacos , Colágeno/farmacología , Proteínas del Esmalte Dental/farmacología , Minerales/farmacología , Extracción Dental , Adulto , Anciano , Implantación Dental Endoósea , Femenino , Humanos , Masculino , Persona de Mediana Edad , Proyectos Piloto , Cicatrización de Heridas/efectos de los fármacosRESUMEN
The transforming growth factor ß (TGF-ß) is thought to have important roles in several stages of folliculogenesis. Vitamin D is effective in cell proliferation, differentition and on estrogen biosynthesis. The aim of the present study was to determine the respective role of 1,25-dihydroxyvitamin D3 on expression of TGF-ß1 in developing rat ovaries. 24 (one-mounth-old n = 12 and adult n = 12) female Wistar rats were enrolled in this study. All animals were divided into 4 groups. Group I and II that consist of one-month-old (n = 6) and adult rats (n = 6) respectively served as control groups. Intramuscular vitamin D3 ( 0,05 µgr/kg/every other day) was injected for 8 weeks to group III and IV which consist of one-mounth-old and adult rats respectively. After last injection, ovaries of animals were removed and processed for immunohistochemistry assay. No remarkable differences in staining intensity and localization for TGF-ß1 were observed in group I and group III. TGF immunostaining was also predominantly found in oocytes. In granulosa cells, TGF-ß1 immunoreactivity was negative. TGF-ß1 immunostaining were observed both in nuclei and cytoplasm of granulosa cells in group II. But in group IV granulosa cells and oocytes were negative for TGF-ß1. We found that vitamin D administration resulted in a decrease in TGF-ß1 levels in the adult rats, but, TGF-ß1 expression did not significantly decrease in the newborn rats. However, in multiple linear regression analysis, TGF-ß1 expressions were independently associated with vitamin D administration. It is observed that Vitamin D attenuated TGF-ß1 expression. The results of this study suggest that vitamin D may play role in folliculogenesis via TGF-ß1.