RESUMEN
An alternative method for labeling fully replicative enveloped viruses was developed, in which both the biosynthesis and metabolic incorporation of phospholipids in host cells were simultaneously utilized to introduce an azide group to the envelope of the vaccinia virus by taking advantage of the host-derived lipid membrane formation mechanism. Such an azide group could be subsequently used to fluorescently label the envelope of the virus via a bioorthogonal reaction. Furthermore, simultaneous dual-labeling of the virus through the virus replication was realized skillfully by coupling this envelope labeling strategy with "replication-intercalation labeling" of viral nucleic acid. For the first time, it is by natural propagation of the virus in its host cells in the presence of fluorophores that simultaneous dual-labeling of living viruses can be mildly realized with high efficiency in facile and mild conditions.
Asunto(s)
Subtipo H9N2 del Virus de la Influenza A/metabolismo , Fosfatidilcolinas/biosíntesis , Fosfatidilcolinas/metabolismo , Coloración y Etiquetado/métodos , Virus Vaccinia/metabolismo , Alquinos/química , Animales , Azidas/química , Materiales Biocompatibles/química , Chlorocebus aethiops , Perros , Subtipo H9N2 del Virus de la Influenza A/fisiología , Células de Riñón Canino Madin Darby , Viabilidad Microbiana , Fosfatidilcolinas/química , Virus Vaccinia/fisiología , Células Vero , Replicación ViralRESUMEN
A new, efficient Cu-catalyzed intramolecular C-H oxidation/acylation method has been developed for the synthesis of substituted indoline-2,3-diones (isatins). In the presence of CuCl(2) and O(2), a variety of formyl-N-arylformamides underwent the tandem reaction to afford the corresponding indoline-2,3-diones in moderate to good yields. It is noteworthy that the reaction serves as the first example of transition-metal-catalyzed transformation for the preparation of indoline-2,3-diones.
Asunto(s)
Carbono/química , Cobre/química , Formamidas/química , Hidrógeno/química , Indoles/química , Acilación , Catálisis , Oxidación-ReducciónRESUMEN
The title compound, H(3)O(+)·C(2)H(8)NO(6)P(2) (-), contains a disordered H(3)O(+) cation and an NH(3)C(CH(3))(PO(3)H)(2) anion. The three H atoms of the H(3)O(+) cation are statistically distributed over four positions with occupancies of 0.75, resulting in a pseudo tetra-hedron. Multiple N-Hâ¯O and O-Hâ¯O hydrogen bonds generate an intricate three-dimensional network.
RESUMEN
Efficient and solvent-free copper-catalyzed N-arylations of imidazoles with aryl and heteroaryl halides have been demonstrated. In the presence of CuBr, 2-aminopyrimidine-4,6-diol, and TBAF (n-Bu4NF), a variety of imidazoles underwent the N-arylation reaction with aryl and heteroaryl halides smoothly in moderate to excellent yields. Noteworthy is that the reaction is conducted under solvent-free conditions.
Asunto(s)
Imidazoles/química , Pirimidinas/química , Alcoholes , Catálisis , Cobre , Hidrocarburos Halogenados , LigandosRESUMEN
[reaction: see text] An efficient Pd(OAc)2/Dabco-catalyzed Stille cross-coupling reaction procedure has been developed. In the presence of Pd(OAc)2 and Dabco (triethylenediamine), various aryl halides including aryl iodides, aryl bromides, and activated aryl chlorides were coupled efficiently with organotin compounds to afford the corresponding biaryls, alkene, and alkynes in good to excellent yields. Furthermore, high TONs [turnover numbers, up to 980,000 TONs for the coupling reaction of 1-bromo-4-nitrobenzene and furan-2-yltributyltin] for the Stille cross-coupling reaction were observed.
RESUMEN
Carbon dioxide was found to promote the palladium-catalyzed zinc-mediated reductive Ullmann coupling of aryl halides. In the presence of carbon dioxide, Pd/C, and zinc, various aromatic halides including less reactive aromatic chlorides were coupled to give the corresponding homocoupling products in good yields.