Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 4 de 4
Filtrar
Más filtros










Base de datos
Intervalo de año de publicación
1.
Animals (Basel) ; 14(13)2024 Jun 22.
Artículo en Inglés | MEDLINE | ID: mdl-38997964

RESUMEN

This experiment was carried out to investigate the effect of pterostilbene (PTE) supplementation in feed on Arbor Acres broilers in terms of serum biochemical parameters, immune and inflammatory responses, antioxidant status, and intestinal morphological structure. For a duration of 42 days, a total of 480 1-day-old Arbor Acres broilers were randomly divided into four groups. Each group was assigned to receive either the basal diet or the basal diet supplemented with 200, 400, or 600 mg/kg of PTE. Each treatment consisted of eight replicates, with 15 chicks per replicate. In comparison with the control group, three PTE treatments significantly increased the lymphocyte transformation rate in the spleen of broilers. The automated biochemical analysis, enzyme-linked immunosorbent assay, and RT-qPCR analysis kits found that 400 mg/kg of PTE significantly increased the serum levels of complement C3, IL-4, and iNOS; reduced the serum levels of IL-6, TNF-α, and mRNA levels of the genes IL-6, IL-8, TNF-α, NLRP3, and IFN-γ; significantly improved the activities of antioxidant enzymes including CAT, GSH-Px, and T-SOD in the jejunum; and significantly reduced the MDA contents in the serum and jejunum of broilers. Nikon microscope observations and ImagePro Plus 6.0 measure results found that 400 mg/kg of PTE supplementation significantly reduced the relative length and weight of the jejunum and improved the jejunal villi structure, resulting in increased intestinal villi, deepened crypt, and an enhanced ratio of villi height to crypt depth (VH/CD). RT-qPCR and Western blot found that dietary PTE also resulted in increased mRNA levels of the genes Claudin-2, Occludin, ZO-1, and Sirt1, and decreased NF-κB protein levels in the jejunum. The results of this study demonstrated that dietary PTE improved the immune function and intestinal health of broilers by reducing inflammation and increasing the antioxidant capacity of the animals.

2.
J Anim Physiol Anim Nutr (Berl) ; 108(4): 921-933, 2024 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-38372476

RESUMEN

Intensive breeding of broilers met the increasing demands of human for broiler products, but it raised their increased susceptibility to various stressors resulting in the disorder of lipid metabolism. Pterostilbene, the methoxylated analogue of resveratrol, exhibits astonishing functions of antioxidant, anti-inflammatory and glycolipid regulatory. The study aimed to elucidate the protective effects of pterostilbene on broiler liver and to explore the potential mechanisms. A total of 480 one-day-old male Arbor Acres (AA) broilers were randomly divided into four groups: the control group (basal diet) and pterostilbene groups (PT200, PT400, and PT600 feeding with basal diet containing 200, 400 and 600 mg/kg pterostilbene, respectively). The results showed that the dietary pterostilbene supplementation significantly improved the ADG of broilers. Dietary pterostilbene supplementation regulated the expression levels of the genes Sirt1 and AMPK and the downstream genes related to lipid metabolism to protect liver function and reduce lipid accumulation in broilers. Dietary pterostilbene supplementation upregulated the expression levels of the Nrf2 gene and its downstream antioxidant genes (SOD, CAT, HO-1, NQO-1, GPX) and phase II detoxification enzyme-related genes (GST, GCLM, GCLC). Collectively, pterostilbene was confirmed the positive effects as a feed additive on lipid metabolism and antioxidant via regulating Sirt1/AMPK and Nrf2 signalling pathways in broilers.


Asunto(s)
Alimentación Animal , Antioxidantes , Pollos , Dieta , Suplementos Dietéticos , Metabolismo de los Lípidos , Hígado , Estilbenos , Animales , Estilbenos/farmacología , Estilbenos/administración & dosificación , Antioxidantes/farmacología , Antioxidantes/metabolismo , Masculino , Hígado/efectos de los fármacos , Hígado/metabolismo , Dieta/veterinaria , Metabolismo de los Lípidos/efectos de los fármacos , Alimentación Animal/análisis , Regulación de la Expresión Génica/efectos de los fármacos , Fenómenos Fisiológicos Nutricionales de los Animales
3.
J Sci Food Agric ; 103(3): 1550-1560, 2023 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-36208473

RESUMEN

BACKGROUND: Lipopolysaccharides (LPS) are the main pathogenic substances in Gram-negative bacteria. The aim of this study was to investigate the preventive effects of dietary curcumin (CUR) on LPS toxicity in the duck ileum. The duck diet was supplemented with CUR (0.5 g kg-1 ) for 28 days, while the birds were injected with LPS (0.5 mg kg-1 body weight per injection, administered as seven injections in the last week of the experimental period). RESULTS: LPS significantly decreased the ileal villus-to-crypt ratio in the non-supplemented CUR group. Dietary CUR alleviated LPS-induced morphological damage to the ileum. Moreover, dietary CUR alleviated oxidative stress by increasing the levels of total superoxide dismutase (T-SOD) (P < 0.05) and glutathione S-transferase (GST) (P < 0.05) and decreasing the production of malonic dialdehyde (MDA) (P < 0.05) in control ducks and LPS-challenged ducks. Dietary CUR significantly inhibited the LPS-induced massive production of inflammatory factors (IL-1ß, IL-6, and TNF-α) (P < 0.05). CUR induced the inhibition of TLR4 and activation of Nrf2 to reduce the expression of inflammation-related genes (TLR4, NF-κB, IKK, TXNIP, NLRP3, caspase-1, IL-1ß, IL-6, and TNF-α). Moreover, dietary CUR ameliorated the decrease in claudin-1 and occludin expression (P < 0.05) and improved ZO-1 expression in the duck ileum (P < 0.05). CONCLUSION: In conclusion, dietary CUR has beneficial effects on LPS-induced ileal damage, oxidative damage, and inflammatory response by inhibiting the TLR/NF-κB and activating the Nrf2 signaling pathways in ducks. This study provides valuable information regarding the therapeutic uses of CUR in duck ileitis. © 2022 Society of Chemical Industry.


Asunto(s)
Curcumina , Ileítis , Animales , Lipopolisacáridos/efectos adversos , Patos/metabolismo , Curcumina/farmacología , Curcumina/uso terapéutico , FN-kappa B/metabolismo , Factor de Necrosis Tumoral alfa/metabolismo , Interleucina-6/metabolismo , Factor 2 Relacionado con NF-E2/genética , Factor 2 Relacionado con NF-E2/metabolismo , Receptor Toll-Like 4/metabolismo , Receptor Toll-Like 4/uso terapéutico , Inflamación/inducido químicamente , Inflamación/tratamiento farmacológico , Inflamación/prevención & control , Estrés Oxidativo , Ileítis/inducido químicamente , Ileítis/genética , Ileítis/prevención & control
4.
J Food Biochem ; 45(1): e13553, 2021 01.
Artículo en Inglés | MEDLINE | ID: mdl-33171537

RESUMEN

In this work, the effects of substrates on volatile flavor compounds of Shanghai smoked fish (SSF) from grass carp was investigated by head space-solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) by changing the ratios of soy sauce (15%-25%) to white sugar (10%-20%) and replacing white sugar with reducing sugar (glucose, fructose, and ribose). The results showed the key volatile flavor compounds (ROAV ≥ 1) of SSF were 2,4-decadienal, p-xylene, nonanal, and 1-octen-3-ol with the relative contents of 10.33, 1.14, 4.84, and 1.76%, respectively. Furthermore, the existence of soy sauce had an enhancing role in the production of pyrazines, but no significant difference in white sugar. The contents of isovaleraldehyde and benzeneacetaldehyde were increased when white sugar was replaced with glucose, octanol, and 2-pentyl furan for fructose, no obvious difference in ribose. Moreover, the optimal ratios of soaking solutions were 20% soy sauce and 15% white sugar based on the scoring method of sensory evaluation. This study will provide a theoretical basis for the formation of volatile flavor compounds of SSF. PRACTICAL APPLICATIONS: Grass carp usually grows in freshwater such as pond or lake, but bacteria with earthy smell are easily attached to plankton such as diatom and cyanobacteria leading to the accumulation of bad odor substances through the food chain. Shanghai smoked fish (SSF) deeply loved by public is a traditional special dish with crispy crust and delicious taste. The attractive flavor of grass carp could be increased with the help of the Maillard reaction (MR) and seasonings. Therefore, the effect of the MR on the volatile flavor compounds of SSF was investigated by HS-SPME-GC/MS in this work. A detailed study on the volatile flavor compounds of Shanghai smoked fish could not only enrich the theoretical knowledge of flavor chemistry of freshwater fish, but have a profound contribution to the development of freshwater fish processing techniques.


Asunto(s)
Gusto , Compuestos Orgánicos Volátiles , China , Aromatizantes , Humo , Compuestos Orgánicos Volátiles/análisis
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA