Genome survey and characteristic analysis of SSR in Callicarpa nudiflora / 中国中药杂志

Callicarpa nudiflora,which is a big brand of Li nationality medicine with Hainan characteristics,has the effects of dissolving stasis,hemostasis,anti-inflammatory and antibacterial. At present,there is a lack of information about the reference genome of C. nudiflora. The study of the genome size,heterozygosity rate and characteristics of SSR of C. nudiflora,can provide an effective basis for the formulation of the whole genome de novo sequencing strategy and development of SSR molecular markers of C. nudiflora. To realize this purpose,high throughput sequencing platform Illumina Hiseq was used to sequence the genome structure of C. nudiflora and K-mer analysis was applied to estimate genome size,repeat sequences and heterozygosity rate. Simple-sequence repeat( SSR) loci that are suitable as markers were identified by MISA software. The results showed the estimated genome size of C. nudiflora was 822. 43 Mb,with a 0. 85% heterozygosity rate and 71. 67% repeats,and the GC content of genome was about 49. 20%. Therefore,C. nudiflora belongs to a complex genome with high heterozygosity and repetition. SSR molecular genetic markers were analyzed in the genome sequence,and a total of 206 049 SSRs were identified,among which mono-nucleotide,di-nucleotide and tri-nucleotide repetitive motifs summed up to 198 993,accounting for 96. 57% of the total SSRs. Among the 2-6 nucleotide repeats,AT/AT,AAT/ATT,AGCC/CTGG,AAAAT/ATTTT and AGATAT/ATATCT have the largest number,respectively. This report represents the first genome-wide characterization of C. nudiflora,and provides a reference for the construction of the library for the fine sequencing of the genome,and a molecular basis for the development of SSR molecular markers as well as for the protection and utilization of gene resources.

Studies on residues of organochlorine pesticides and heavy metals in soil of planting base and Pogostemon cablin / 中国中药杂志

<p><b>OBJECTIVE</b>To determine and evaluate the residuals of organochlorine pesticides and heavy metals in soil and in Pogostemon cablin in order to provide for GAP planting.</p><p><b>METHOD</b>GC method was applied to determine residuals of organochlorine pesticides, the contents of Pb, Cd, Cu, Cr, Hg and As were determined by IPC.</p><p><b>RESULT</b>The contents of residuals of organochlorine pesticides and heavy metals were different among three cultivars and planting bases clearly, but all of the residuals in soil from three planting bases were below the second standard of Environmental quality standard for soils (GB15618), and the residuals in P. cablin also meet the "Green standards of medicinal plants and preparations for foreign trade and economy".</p><p><b>CONCLUSION</b>Residuals of organochlorine pesticides and heavy metals in soil and P. cablin from three planting bases in Hainan province were conformed to GAP.</p>

Advances in studies compatibility applied toattenuation and synergy of Tripterygium wilfordii / 中国中药杂志

Review the research and development status that Chinese medicine are compatible with Tripterygium wilfordii for attenuation and synergy for recent year. From modern medicine view and Chinese medicine dialectical perspective explain the mechanisms and methods of compatibility applied to attenuation and synergy of T. wilfordii. Provide a reference for reasonable application of other toxic Chinese medicine. Prefer the suggestion that Chinese medicinal formulae can be developed into Chinese medicine compound preparation.

Textual research on history of introduction corrected and herbal medicine of Pogostemon cablin / 中国中药杂志

<p><b>OBJECTIVE</b>To study the history of introduction and herbal medicine of Pogostemon cablin by textual researches and clarify the confusion in literatures.</p><p><b>METHOD</b>Textual research on historical literatures, practical applications and field investigation were adopted.</p><p><b>RESULT</b>The varieties of P. cablin for therapeutic uses were not chaotic, however there has existed confusion between P. cablin and Agastache rugosus in descriptions of appellation, habitat and characteristic of the original plants.</p><p><b>CONCLUSION</b>It was proved that the term "Huoxiang" in Chinese medicinal works before Ming Dynasty was pointed to P. cablin. The cultivation history of P. cablin in China was traced to Liang Dynasty or before.</p>