The Effects of Players' Rotations on High-Intensity Activities in Professional Futsal Players.

The current study aimed to investigate the effects of interchange rotations on players` physical performance during competition, with special reference to high-intensity activity (HIA) according to the playing position. Physical performance data, collected from 19 professional players during seven official matches from the Spanish futsal league using a portable local positioning system, included the number of high-speed running activities (>18 km·h-1), high-intensity accelerations (>3 m·s-2), and high-intensity decelerations (>3 m·s-2). Statistically significant (p ≤ 0.05) differences were observed in the number of HIA efforts across rotations and between positions. Players performed more HIA efforts in the first rotation (n = 17.6), suggesting that their first rotation was more demanding than all subsequent rotations. Wingers demonstrated a higher HIA effort and frequency of HIA efforts when compared to defenders (p ≤ 0.05) and pivots (p ≤ 0.001). For all positions, the first rotation was more physically demanding as the number of HIA efforts per rotation decreased with an increased number of rotations throughout the match. Furthermore, higher HIA profile positions, such as wingers and defenders, were less likely to maintain consistent HIA properties (repetition number, time-frequency, and the work-rate) across subsequent rotations during the match. The findings of the study can inform coaching decisions regarding players' rotations to maintain consistent HIA performance throughout the match.

How spatial constraints afford successful and unsuccessful penetrative passes in elite association football.

BACKGROUND: The aim of the present study was to examine the spatial relations between teams (macro-level) and groups of players (meso-level) that afford successful penetrative passes (off-ball advantage) in elite football. METHODS: Three balanced home matches from a Premier League team with 91 ball possessions in which a pass was performed into the opposition defensive area and overpassed the first defensive line, promoting a perturbation of the defensive team equilibrium, were selected for analysis. The spatial relations between teams were measured through spatial variables that captured the areas occupied by the teams, while the spatial relations between players were measured through variables that captured the distances and angles between attacking and defending players near the ball. RESULTS: Results revealed, at the macro-level, higher values of width ratio between teams and the width of the attacking team for unsuccessful penetrative passes (UPP), when compared to successful penetrative passes (SPP). At the meso-level, a general decrease in distances and an increase in angles between attacking and defending players were observed between successful to unsuccessful penetrative passes. CONCLUSIONS: These findings highlight the importance of using positional data analysis to identify teams' tactical profiles and to potentiate coaches' interventions.

Intensity demands and peak performance of elite soccer referees during match play.

OBJECTIVES: This study examined the peak physical and physiological (heart rate) performance intensities andassociated decrements in elite soccer referees during match play. DESIGN: Longitudinal study. METHODS: Physical performancevariables and heart rate were analyzed during 457 matches across two seasons. Differences between halves, and the rate ofdecline in peak performance intensities across moving average durations of 1-10 minutes were assessed using linear mixed models and power-law analysis, respectively. RESULTS: Large significant differences were observed between halves for mean total distance, mean velocity, mean heart rate, and percentage of maximal heart rate (p ≤ 0.05; r = 0.51-0.64). Peak intensities (p ≤ 0.05; r = 0.15-0.17) and the rate of decline (p < 0.001; r = 0.17-0.37) were significantly higher in the 2nd half compared to the 1st half, for relative total distance, relative high-intensity running and mean velocity. The rate of decline was significantly greater in the 2nd half than the 1st half for relative distance covered by high-intensity acceleration (>2 m/s-2/min), deceleration (<-2 m/s-2/min), and relative mean heart rate (p < 0.001; r = 0.28-0.61). Elite soccer referees might have experienced transient fatigue during match play, as relative high-intensity running immediately following the most intense 5-minute period significantly declined by 61.2% ( p< 0.001; r = 0.94), and was 16.2% lower than the mean 5-minute period (p < 0.001; r = 0.34). CONCLUSIONS: Increased physical and physiological demands during match play, with associated declines in the second half and transient signs of fatigue throughout the match, supports the inclusion of high-intensity interval and endurance training programs to prepare soccer referees for the intensity demands and peak performance outcomes of match play.

Physical workload and fatigue pattern characterization in a top-class women's football national team: a case study of the 2019 FIFA Women's World Cup.

BACKGROUND: With the growing scientific interest in women's football it is critical to understand the match demands and fatigue patterns during a top-class women's competition. METHODS: Physical characteristics and performance of top-class women football matches during the 2019 FIFA Women's World Cup was investigated from data collected using global positioning system for 21 outfield players during the tournament. RESULTS: Relative total distance (TD; m·min-1) was moderately lower (P≤0.05, ES:0.6) in the final match (96.1±5.0 m·min-1) compared to the first (104.1±5.9 m·min-1) match. Performance in relative total high-speed running (THSR; m·min-1) declined -27.5% (P≥0.05) throughout the tournament following peak performance in the third match (M3) (5.3±1.7 m·min-1). Match performance between halves was reduced -25.4±0.13% (P≤0.05, ES:1.2-2.3) across all measures in M3. Relative high-intensity running (HIR; 25.9±3.5 m·min-1) and THSR (9.8±1.5 m·min-1) were greater (P≤0.05, ES:0.2-0.7) for forwards, while midfielders performed greater (P≤0.05, ES:0.6) relative total distance (102.0±5.5 m·min-1), compared to central defenders (HIR: 16.1±3.7 m·min-1; THSR: 3.1±1.6 m·min-1; TD: 92.5±5.7 m·min-1). Reductions in relative HIR between halves (-10.9%), within the first half (-31.0%), and the start and end of the match (-36.9%) were most pronounced for midfielders (P≤0.05, ES:0.6-2.3). Across all matches, relative THSR performance was reduced, with moderate to large differences (P≤0.001, ES: 0.7-0.8) observed between halves, within the first half, and throughout the match. CONCLUSIONS: In conclusion, specific fatigue patterns observed within and between matches throughout the women's World Cup, may be influenced by playing positions, the rank of the opposition, as well as the stage of the tournament.

Testosterone enables growth and hypertrophy in fusion impaired myoblasts that display myotube atrophy: deciphering the role of androgen and IGF-I receptors.

We have previously highlighted the ability of testosterone (T) to improve differentiation and myotube hypertrophy in fusion impaired myoblasts that display reduced myotube hypertrophy via multiple population doublings (PD) versus their parental controls (CON); an observation which is abrogated via PI3K/Akt inhibition (Deane et al. 2013). However, whether the most predominant molecular mechanism responsible for T induced hypertrophy occurs directly via androgen receptor or indirectly via IGF-IR/PI3K/Akt pathway is currently debated. PD and CON C2C12 muscle cells were exposed to low serum conditions in the presence or absence of T (100 nM) ± inhibitors of AR (flutamide/F, 40 µm) and IGF-IR (picropodophyllin/PPP, 150 nM) for 72 h and 7 days (early/late muscle differentiation respectively). T increased AR and Akt abundance, myogenin gene expression, and myotube hypertrophy, but not ERK1/2 activity in both CON and PD cell types. Akt activity was not increased significantly in either cell type with T. Testosterone was also unable to promote early differentiation in the presence of IGF-IR inhibitor (PPP) yet still able to promote appropriate later increases in myotube hypertrophy and AR abundance despite IGF-IR inhibition. The addition of the AR inhibitor powerfully attenuated all T induced increases in differentiation and myotube hypertrophy with corresponding reductions in AR abundance, phosphorylated Akt, ERK1/2 and gene expression of IGF-IR, myoD and myogenin with increases in myostatin mRNA in both cell types. Interestingly, despite basally reduced differentiation and myotube hypertrophy, PD cells showed larger T induced increases in AR abundance vs. CON cells, a response abrogated in the presence of AR but not IGF-IR inhibitors. Furthermore, T induced increases in Akt abundance were sustained despite the presence of IGF-IR inhibition in PD cells only. Importantly, flutamide alone reduced IGF-IR mRNA in both cell types across time points, with an observed reduction in activity of ERK and Akt, suggesting that IGF-IR was transcriptionally regulated by AR. However, where testosterone increased AR protein content there was no increases observed in IGF-IR gene expression. This suggested that sufficient AR was important to enable normal IGF-IR expression and downstream signalling, yet elevated levels of AR due to testosterone had no further effect on IGF-IR mRNA, despite testosterone increasing Akt abundance in the presence of IGF-IR inhibitor. In conclusion, testosterones ability to improve differentiation and myotube hypertrophy occurred predominately via increases in AR and Akt abundance in both CON and PD cells, with fusion impaired cells (PD) showing an increased responsiveness to T induced AR levels. Finally, T induced increases in myotube hypertrophy (but not early differentiation) occurred independently of upstream IGF-IR input, however it was apparent  that normal AR function in basal conditions was required for adequate IGF-IR gene expression and downstream ERK/Akt activity.