Viral lysing can alleviate microbial nutrient limitations and accumulate recalcitrant dissolved organic matter components in soil.

Viruses are critical for regulating microbial communities and biogeochemical processes affecting carbon/nutrient cycling. However, the role of soil phages in controlling microbial physiological traits and intrinsic dissolved organic matter (DOM) properties remains largely unknown. Herein, microcosm experiments with different soil phage concentrates (including no-added phages, inactive phages, and three dilutions of active phages) at two temperatures (15 °C and 25 °C) were conducted to disclose the nutrient and DOM dynamics associated with viral lysing. Results demonstrated three different phases of viral impacts on CO2 emission at both temperatures, and phages played a role in maintaining Q10 within bounds. At both temperatures, microbial nutrient limitations (especially P limitation) were alleviated by viral lysing as determined by extracellular enzyme activity (decreased Vangle with active phages). Additionally, the re-utilization of lysate-derived DOM by surviving microbes stimulated an increase of microbial metabolic efficiency and recalcitrant DOM components (e.g., SUV254, SUV260 and HIX). This research provides direct experimental evidence that the "viral shuttle" exists in soils, whereby soil phages increase recalcitrant DOM components. Our findings advance the understanding of viral controls on soil biogeochemical processes, and provide a new perspective for assessing whether soil phages provide a net "carbon sink" vs. "carbon source" in soils.

Influence of tetracycline on arsenic mobilization and biotransformation in flooded soils.

This study examined the effect of tetracycline addition on arsenic (As) mobilization and biotransformation in two contrasting soils (upland soil and paddy soil) under flooded conditions. The soils with added tetracycline (0-50 mg kg-1) were incubated for 30 days, and soil properties and microbial functional genes over time were quantified. Tetracycline significantly promoted As reduction and As release into porewater in both soils. The enhancement had resulted from an increase in the concentration of dissolved organic carbon and a decrease in soil redox potential. Tetracycline also increased the abundances of As-reducing genes (arsC and arrA) and the relative abundances of As-reducing bacteria Streptomyces, Bacillus, Burkholderia, Clostridium and Rhodococcus, all of which have been found resistant to tetracycline. These genera play a key part in stimulating As reduction in the presence of tetracycline. The study indicated the significance of tetracycline in the biochemical behavior of As in flooded soils and provided new insights into the potential effects of tetracycline on the quality and safety of agricultural products in the future.

Habitat heterogeneity induced by pyrogenic organic matter in wildfire-perturbed soils mediates bacterial community assembly processes.

Although pyrogenic organic matter (PyOM) generated during wildfires plays a critical role in post-fire ecosystem recovery, the specific mechanisms by which PyOM controls soil microbial community assembly after wildfire perturbation remain largely uncharacterized. Herein we characterized the effect of PyOM on soil bacterial communities at two independent wildfire-perturbed forest sites. We observed that α-diversity of bacterial communities was the highest in wildfire-perturbed soils and that bacterial communities gradually changed along a sequence of unburnt soil → burnt soil → PyOM. The microbial communities reconstructed from unburnt soil and PyOM resembled the real bacterial communities in wildfire-perturbed soils in their α-diversity and community structure. Bacterial specialists in PyOM and soils clustered in phylogenetic coherent lineages with intra-lineage pH-niche conservatism and inter-lineage pH-niche divergence. Our results suggest that PyOM mediates bacterial community assembly in wildfire-perturbed soils by a combination of environmental selection and dispersal of phylogenetic coherent specialists with habitat preference in the heterogeneous microhabitats of burnt soils with distinct PyOM patches.

The effects of functional groups on the sorption of naphthalene on microplastics.

Ubiquitous microplastics were recognized as an ideal carrier for polycyclic aromatic hydrocarbons (PAHs) to spread in nature or transfer to biota, thus posing risks to human health. However, little is known about how the functional groups in PAH derivatives influence their sorption onto microplastics as compared to parent PAHs. This study investigated the sorption of naphthalene (NAP) and its derivatives onto polystyrene microspheres with (MP-COOH) or without (MP) surface modification of the carboxyl group. NAP derivatives with charged groups (e.g., -NH2, -OH and -COOH) reached the sorption equilibrium more early than NAP and its derivative with an uncharged group (e.g., -CH3), whereas their sorption capacities (Kd = 6.0-8.4 L/g for MP, Kd = 4.5-6.3 L/g for MP-COOH) were significantly lower than that of the latter (Kd = 11.6-12.0 L/g for MP, Kd = 9.4-10.0 L/g for MP-COOH). The inhibition of charged groups on sorption could be attributed to their facilitation on molecular polarity and, thus, their lower hydrophobicity (LogKOW) since hydrophobicity was the crucial factor controlling the sorption of NAP and NAP derivatives onto both MP and MP-COOH. Computational modeling further showed that charged functional groups would weaken the π-π interaction or strengthen the repulsion between NAP or NAP derivative molecule and MP or MP-COOH molecule, which might also contribute to the suppressed sorption. This study provides new insight into the affinity of PAH derivatives with microplastics, which, therefore, improves our understanding of the environmental fates of microplastics and the organic pollutants.

Spatiotemporal modeling of soil heavy metals and early warnings from scenarios-based prediction.

Prediction of soil heavy metal concentrations based on continuous site specific investigation can provide reference for soil metal contamination prevention and early warning of soil environmental quality. In this study, the spatiotemporal variations of soil heavy metals (Cd, Ni, Zn, Pb and Cu) in Wenling were analyzed with 132 and 169 soil samples gathered in 2011 and 2016. In addition, we adopted a scenario-simulation model to predict future dynamic concentrations of soil heavy metals under optimistic (the pollution inputs are zero under strict environmental policy) and default (the pollution status maintain constant) conditions. Results indicated that the paddy soil was contaminated mainly by Cd and Cu. Spatiotemporal maps revealed distinct patterns in the joint area, where soil Cd, Ni, Zn, Pb and Cu all increased in northwest. Soil heavy metal concentrations as well as the associated ecological risks would decline gradually under optimistic scenario, while sharply increase when no control acts are taken over long term in default condition. The percentages of soil Cd and Cu that exceeding their corresponding risk screening value (RSV) under the default condition would be 1.6 and 1.3 times higher than those under optimistic scenario 10 years later. The probability of high potential ecological risk in default condition would be twice higher than that under optimistic scenario in 2026. Overall, strengthening the control of pollution sources and strict environmental policy are very important for soil heavy metals contamination prevention and control.