RESUMEN
Slow dissolution/hydrolysis of insoluble/macromolecular organics and poor sludge filterability restrict the application potential of anaerobic membrane bioreactor (AnMBR). Bubble-free membrane microaeration was firstly proposed to overcome these obstacles in this study. The batch anaerobic digestion tests feeding insoluble starch and soluble peptone with and without microaeration showed that microaeration led to a 65.7-144.8% increase in methane production and increased critical flux of microfiltration membrane via driving the formation of large sludge flocs and the resultant improvement of sludge settleability. The metagenomic and bioinformatic analyses showed that microaeration significantly enriched the functional genes and bacteria for polysaccharide and protein hydrolysis, microaeration showed little negative effects on the functional genes involved in anaerobic metabolisms, and substrate transfer from starch to peptone significantly affected the functional genes and microbial community. This study demonstrates the dual synergism of microaeration to enhance the dissolution/hydrolysis/acidification of insoluble/macromolecular organics and sludge filterability for AnMBR application.
Asunto(s)
Reactores Biológicos , Filtración , Membranas Artificiales , Aguas del Alcantarillado , Reactores Biológicos/microbiología , Aguas del Alcantarillado/microbiología , Anaerobiosis , Filtración/métodos , Metano/metabolismo , Hidrólisis , Almidón/metabolismoRESUMEN
OBJECTIVE: To investigate the mechanistic basis for the anti-proliferation and anti-invasion effect of tumor necrosis factor-related apoptosis-induced ligand (TRAIL) and celastrol combination treatment (TCCT) in glioblastoma cells. METHODS: Cell counting kit-8 was used to detect the effects of different concentrations of celastrol (0-16 µmol/L) and TRAIL (0-500 ng/mL) on the cell viability of glioblastoma cells. U87 cells were randomly divided into 4 groups, namely control, TRAIL (TRAIL 100 ng/mL), Cel (celastrol 0.5 µmol/L) and TCCT (TRAIL 100 ng/mL+ celastrol 0.5 µmol/L). Cell proliferation, migration, and invasion were detected by colony formation, wound healing, and Transwell assays, respectively. Quantitative reverse transcription polymerase chain reaction and Western blotting were performed to assess the levels of epithelial-mesenchymal transition (EMT) markers (zona occludens, N-cadherin, vimentin, zinc finger E-box-binding homeobox, Slug, and ß-catenin). Wnt pathway was activated by lithium chloride (LiCl, 20 mol/L) and the mechanism for action of TCCT was explored. RESULTS: Celastrol and TRAIL synergistically inhibited the proliferation, migration, invasion, and EMT of U87 cells (P<0.01). TCCT up-regulated the expression of GSK-3ß and down-regulated the expression of ß-catenin and its associated proteins (P<0.05 or P<0.01), including c-Myc, Cyclin-D1, and matrix metalloproteinase (MMP)-2. In addition, LiCl, an activator of the Wnt signaling pathway, restored the inhibitory effects of TCCT on the expression of ß-catenin and its downstream genes, as well as the migration and invasion of glioblastoma cells (P<0.05 or P<0.01). CONCLUSIONS: Celastrol and TRAIL can synergistically suppress glioblastoma cell migration, invasion, and EMT, potentially through inhibition of Wnt/ß-catenin pathway. This underlies a novel mechanism of action for TCCT as an effective therapy for glioblastoma.
Asunto(s)
Glioblastoma , Triterpenos Pentacíclicos , Vía de Señalización Wnt , Humanos , Glioblastoma/tratamiento farmacológico , Glioblastoma/patología , beta Catenina/metabolismo , Glucógeno Sintasa Quinasa 3 beta/metabolismo , Ligandos , Línea Celular Tumoral , Apoptosis , Factores de Necrosis Tumoral/farmacología , Proliferación Celular , Movimiento Celular , Transición Epitelial-MesenquimalRESUMEN
Effect of ferrate [Fe(VI)] pre-oxidation on improving FeCl3/ultrafiltration (UF) of algae-laden source water was investigated. Fe(VI) disrupted algae cells and the in situ formed ferric (hydr)oxides aggregated with cell debris. Particle size and zeta potential of algae increased by 20% and 55% on average, respectively, after treatment with 0.02 mM of Fe(VI). These variations facilitated the formation of algae-ferric floc. Fe(VI) degraded algal extracellular organic matter into lower molecular weight products (fulvic-like and humic-like substances). Membrane flux, reversible membrane resistance (Rr) and irreversible membrane resistance (Rir) were improved by 51%, 61%, and 52% in Fe(VI) (0.02 mM)/FeCl3/UF treatment group compared with FeCl3/UF treatment after three filtration cycles. Fe(VI)/FeCl3/UF removed more than 10% ~ 34% of the dissolved organic compounds (DOC) and 6% ~ 17% of the total nitrogen (TN) compared with FeCl3/UF. Due to the enhanced removal of DOC and TN, formation potential of 12 kinds of carbonaceous-disinfection byproducts (C-DBPs) and 7 kinds of nitrogenous-disinfection byproducts (N-DBPs) decreased by 32.5% and 22.5%, respectively. Fe(VI) pre-oxidant was effective for alleviating membrane fouling and reducing formation potential of DBPs in algal laden water treatment.
Asunto(s)
Ultrafiltración , Purificación del Agua , Desinfección , Hierro , AguaRESUMEN
Elemental sulfur (S0) is always produced during bio-denitrification and desulfurization process, but the S0 yield and purification quality are too low. Till now, no feasible approach has been carried out to efficiently recover S0. In this study, we report the S0 generation and recovery by a newly designed, compact, biofilm formed membrane filtration reactor (BfMFR), where S0 was generated within a Thauera sp. strain HDD-formed biofilm on membrane surface, and then timely separated from the biofilm through membrane filtration. The high S0 generation efficiency (98% in average) was stably maintained under the operation conditions with the influent acetate, nitrate and sulfide concentration of 115, 120 and 100 mg/L, respectively, an initial inoculum volume of approximate 2.4 × 108 cells, and a membrane pore size of 0.45 µm. Under this condition, the sulfide loading approached 62.5 kg/m3·d, one of the highest compared with the previous reports, demonstrating an efficient sulfide removal and S0 generation capacity. Particular important, a solid analysis of the effluent revealed that the recovered S0 was adulterated with barely microorganisms, extracellular polymeric substances (EPSs), or inorganic chemicals, indicating a fairly high S0 recovery purity. Membrane biofilm analysis revealed that 80.7% of the generated S0 was accomplished within 45-80 µm of biofilm from the membrane surface and while, the complete membrane fouling due to bacteria and EPSs was generally observed after 14-16 days. The in situ generation and timely separation of S0 from the bacterial group by BfMFR, effectively avoids the sulfur circulation (S2- to S0, S0 to SO42-, SO42- to HS-) and guarantees the high S0 recovery efficiency and purity, is considered as a feasible approach for S0 recovery from sulfide- and nitrate-contaminated wastewater.
Asunto(s)
Desnitrificación , Reciclaje/métodos , Azufre/análisis , Aguas Residuales/química , Biopelículas , Reactores Biológicos/microbiología , Nitratos/análisis , Nitratos/química , Sulfuros/análisis , Sulfuros/química , Azufre/químicaRESUMEN
BACKGROUND: Several G-protein-coupled receptors (GPCRs) have been shown to be important signaling mediators between neurons and glia. In our previous screening for identification of nerve injury-associated GPCRs, G-protein-coupled receptor 84 (GPR84) mRNA showed the highest up-regulation by microglia after nerve injury. GPR84 is a pro-inflammatory receptor of macrophages in a neuropathic pain mouse model, yet its function in resident microglia in the central nervous system is poorly understood. METHODS: We used endogenous, natural, and surrogate agonists for GPR84 (capric acid, embelin, and 6-OAU, respectively) and examined their effect on mouse primary cultured microglia in vitro. RESULTS: 6-n-Octylaminouracil (6-OAU), embelin, and capric acid rapidly induced membrane ruffling and motility in cultured microglia obtained from C57BL/6 mice, although these agonists failed to promote microglial pro-inflammatory cytokine expression. Concomitantly, 6-OAU suppressed forskolin-induced increase of cAMP in cultured microglia. Pertussis toxin, an inhibitor of Gi-coupled signaling, completely suppressed 6-OAU-induced microglial membrane ruffling and motility. In contrast, no 6-OAU-induced microglial membrane ruffling and motility was observed in microglia from DBA/2 mice, a mouse strain that does not express functional GPR84 protein due to endogenous nonsense mutation of the GPR84 gene. CONCLUSIONS: GPR84 mediated signaling causes microglial motility and membrane ruffling but does not promote pro-inflammatory responses. As GPR84 is a known receptor for medium-chain fatty acids, those released from damaged brain cells may be involved in the enhancement of microglial motility through GPR84 after neuronal injury.
Asunto(s)
Movimiento Celular/efectos de los fármacos , Microglía/efectos de los fármacos , Receptores Acoplados a Proteínas G/agonistas , Receptores Acoplados a Proteínas G/metabolismo , Animales , Animales Recién Nacidos , Benzoquinonas/farmacología , Células Cultivadas , Corteza Cerebral/citología , AMP Cíclico/metabolismo , Citocinas/genética , Citocinas/metabolismo , Ácidos Decanoicos/farmacología , Eliminación de Gen , Macrófagos/efectos de los fármacos , Ratones , Ratones Endogámicos C57BL , Ratones Endogámicos DBA , Microglía/inmunología , Óxido Nítrico Sintasa de Tipo II/metabolismo , Toxina del Pertussis/farmacología , Receptores Acoplados a Proteínas G/genética , Transducción de Señal/efectos de los fármacos , Uracilo/farmacologíaRESUMEN
Selective serotonin reuptake inhibitors (SSRIs) have been reported to increase cognitive performance in some clinical studies of Alzheimer's disease (AD). However, there is a lack of evidence supporting the efficacy of SSRIs as cognition enhancers in AD, and the role of SSRIs as a treatment for AD remains largely unclear. Here, we characterized the impact of fluoxetine (FLX), a well-known SSRI, on neurons in the dentate gyrus (DG) and in CA1 and CA3 of the hippocampus of middle-aged (16 to 17 months old) APPswe/PSEN1dE9 (APP/PS1) transgenic AD model mice. We found that intraperitoneal (i.p.) injection of FLX (10 mg/kg/day) for 5 weeks effectively alleviated the impairment of spatial learning ability in middle-aged APP/PS1 mice as evaluated using the Morris water maze. More importantly, the number of neurons in the hippocampal DG was significantly increased by FLX. Additionally, FLX reduced the deposition of beta amyloid, inhibited GSK-3ß activity and increased the level of ß-catenin in middle-aged APP/PS1 mice. Collectively, the results of this study indicate that FLX delayed the progression of neuronal loss in the hippocampal DG in middle-aged AD mice, and this effect may underlie the FLX-induced improvement in learning ability. FLX may therefore serve as a promising therapeutic drug for AD.
Asunto(s)
Enfermedad de Alzheimer/tratamiento farmacológico , Cognición/efectos de los fármacos , Fluoxetina/uso terapéutico , Aprendizaje por Laberinto/efectos de los fármacos , Degeneración Nerviosa/tratamiento farmacológico , Neuronas/efectos de los fármacos , Inhibidores Selectivos de la Recaptación de Serotonina/uso terapéutico , Enfermedad de Alzheimer/complicaciones , Enfermedad de Alzheimer/patología , Péptidos beta-Amiloides/metabolismo , Precursor de Proteína beta-Amiloide/genética , Animales , Región CA1 Hipocampal/citología , Región CA1 Hipocampal/efectos de los fármacos , Región CA3 Hipocampal/citología , Región CA3 Hipocampal/efectos de los fármacos , Giro Dentado/citología , Giro Dentado/efectos de los fármacos , Giro Dentado/patología , Modelos Animales de Enfermedad , Glucógeno Sintasa Quinasa 3 beta/metabolismo , Humanos , Inmunohistoquímica , Inyecciones Intraperitoneales , Masculino , Ratones , Ratones Transgénicos , Presenilina-1/genética , Memoria Espacial/efectos de los fármacos , beta CateninaRESUMEN
This study was designed to investigate the protective effects of extracellular superoxide dismutase (SOD3) against amyloid beta (Aß25-35)-induced damage in human neuroblastoma SH-SY5Y cells and to elucidate the mechanisms responsible for this beneficial effect. SH-SY5Y cells overexpressing SOD3 were generated by adenoviral vector-mediated infection and Aß25-35 was then added to the cell culture system to establish an in vitro model of oxidative stress. Cell viability, the generation of intracellular reactive oxygen species (ROS), the expression and activity of antioxidant enzymes, the levels of lipid peroxidation malondialdehyde (MDA), the expression of mitochondrial apoptosis-related genes and calcium images were examined. Following Aß25-35 exposure, SOD3 overexpression promoted the survival of SH-SY5Y cells, decreased the production of ROS, decreased MDA and calcium levels, and decreased cytochrome c, caspase-3, caspase-9 and Bax gene expression. Furthermore, SOD3 overexpression increased the expression and activity of antioxidant enzyme genes and Bcl-2 expression. Together, our data demonstrate that SOD3 ameliorates Aß25-35-induced oxidative damage in neuroblastoma SH-SY5Y cells by inhibiting the mitochondrial pathway. These data provide new insights into the functional actions of SOD3 on oxidative stress-induced cell damage.
Asunto(s)
Péptidos beta-Amiloides/toxicidad , Mitocondrias/metabolismo , Estrés Oxidativo/efectos de los fármacos , Fragmentos de Péptidos/toxicidad , Superóxido Dismutasa/metabolismo , Apoptosis/efectos de los fármacos , Calcio/metabolismo , Línea Celular Tumoral , Humanos , Espacio Intracelular/metabolismo , Mitocondrias/efectos de los fármacos , Oxidación-Reducción/efectos de los fármacos , Especies Reactivas de Oxígeno/metabolismoRESUMEN
Olfactory ensheathing cells (OECs) are a type of glia from the mammalian olfactory system, with neuroprotective and regenerative properties. ß-Amyloid peptides are a major component of the senile plaques characteristic of the Alzheimer brain. The amyloid beta (Aß) precursor protein is cleaved to amyloid peptides, and Aß25-35 is regarded to be the functional domain of Aß, responsible for its neurotoxic properties. It has been reported that Aß25-35 triggers reactive oxygen species (ROS)-mediated oxidative damage, altering the structure and function of mitochondria, leading to the activation of the mitochondrial intrinsic apoptotic pathway. Our goal is to investigate the effects of OECs on the toxicity of aggregated Aß25-35, in human neuroblastoma SH-SY5Y cells. For such purpose, SH-SY5Y cells were incubated with Aß25-35 and OEC-conditioned medium (OECCM). OECCM promoted the cell viability and reduced the apoptosis, and decreased the intracellular ROS and the lipid peroxidation. In the presence of OECCM, mRNA and protein levels of antioxidant enzymes (SOD1 and SOD2) were upregulated. Concomitantly, OECCM decreased mRNA and the protein expression levels of cytochrome c, caspase-9, caspase-3, and Bax in SH-SY5Y cells, and increased mRNA and the protein expression level of Bcl-2. However, OECCM did not alter intracellular Ca2+ concentration in SH-SY5Y cells. Taken together, our data suggest that OECCM ameliorates Aß25-35-induced oxidative damage in neuroblastoma SH-SY5Y cells by inhibiting the mitochondrial intrinsic pathway. These data provide new insights into the functional actions of OECCM on oxidative stress-induced cell damage.
Asunto(s)
Péptidos beta-Amiloides/toxicidad , Apoptosis/efectos de los fármacos , Medios de Cultivo Condicionados/farmacología , Mitocondrias/metabolismo , Bulbo Olfatorio/citología , Estrés Oxidativo/efectos de los fármacos , Fragmentos de Péptidos/toxicidad , Transducción de Señal/efectos de los fármacos , Animales , Calcio/metabolismo , Línea Celular Tumoral , Humanos , Mitocondrias/efectos de los fármacos , Oxidación-Reducción , Ratas , Especies Reactivas de Oxígeno/metabolismo , Proteína X Asociada a bcl-2/metabolismoRESUMEN
This study was designed to investigate the protective effects of extracellular superoxide dismutase (SOD3) against hydrogen peroxide (H2O2) induced damage in human neuroblastoma SH-SY5Y cells and to elucidate the mechanisms responsible for this beneficial effect. SOD3-overexpressing SH-SY5Y cells were generated by adenoviral vector-mediated infection, and H2O2 was then added into the cell culture system to establish an in vitro model of oxidative stress. Cell viability, the generation of intracellular reactive oxygen species (ROS), the expression and activity of antioxidant enzymes, the levels of lipid peroxidation malondialdehyde (MDA), the expression of mitochondrial apoptosis-related genes, and calcium imaging were examined. Following H2O2 exposure, the over-expression of SOD3 promoted the survival of SH-SY5Y cells; decreased the production of ROS, MDA levels, cytochrome C, caspase-3, caspase-9, and Bax gene expression, and calcium levels; and increased the expression and activity of antioxidant enzyme genes and the expression level of Bcl-2. Together, our data demonstrate that SOD3 ameliorates H2O2-induced oxidative damage in neuroblastoma SH-SY5Y cells by inhibiting the mitochondrial pathway and provide new insights into the functional actions of SOD3 on oxidative stress-induced cell damage.
Asunto(s)
Peróxido de Hidrógeno/toxicidad , Mitocondrias/metabolismo , Estrés Oxidativo/fisiología , Transducción de Señal/fisiología , Superóxido Dismutasa/biosíntesis , Línea Celular Tumoral , Supervivencia Celular/efectos de los fármacos , Supervivencia Celular/fisiología , Humanos , Mitocondrias/efectos de los fármacos , Estrés Oxidativo/efectos de los fármacos , Transducción de Señal/efectos de los fármacosRESUMEN
OBJECTIVE: To investigate the effects of naringenin on the learning and memory ability of Alzheimer disease (AD) rats. METHODS: 30 male SD rats were randomly divided into control group (sham operation group), model group and naringenin group. AD model was established by injecting strepoztocin (3 mg/kg) twice into each of two intracerebroventriculas. Naringenin group were given intragastric administration of naringenin once a day for 3 weeks and the other two groups were given intragadtric administration of normal saline with the same dosage and time period. After 3 weeks, learning and memory ability in all the three groups were analyzed by Morris water maze, the activity of superoxide dismutase(SOD) and the content of malondialdehyde (MDA) of the rats' brain tissue was detected by chemical colorimetric determination. Observed the expressions of Abeta42 and Abeta40 by immunohistochemical method. The expression and degree of phosphorylation of tau protein was assayed by western blotting. RESULTS: 1. Compared with the sham operation group, the mean escape latency of the model group was significantly prolonged (P < 0.05) and the time that rats were in the platform quadrant was significantly shortened (P < 0.0.5). On the contrary, compared with the model group, the mean escape latency of naringenin group was significantly shortened (P < 0.05) and the time that rats were in the platform quadrant was significantly extended (P < 0.005). 2. The level of MDA in the model group, compared with the sham operation group group, was significantly increased (P < 0.05). Whereas, that of naringenin group, compared with the model group, was significantly decreased compared with the sham operation group (P < 0.05). The activity of SOD in the naringenin group was significantly increased comparing with the model group (P < 0.05). 3. The expressions of Abeta40 and Abeta42 in model group were obviously up-regulated. Instead, the expressions of Abeta40 and Abeta42 in the naringenin group were significantly down regulated. 4. There was no significant difference in the expression of tau protein among each groups. Nevertheless, the phosphorylation of tau protein in the model group was significantly enhanced than that in the control group (P < 0.05), and the phosphorylation of tau protein in the naringenin group was significantly reduced than that in the model group (P < 0.05). CONCLUSION: Naringenin can improve learning and memory ability of model rats with Alzheimer disease through the approach of oxidative stress.
Asunto(s)
Enfermedad de Alzheimer/tratamiento farmacológico , Antioxidantes/farmacología , Encéfalo/efectos de los fármacos , Flavanonas/farmacología , Aprendizaje/efectos de los fármacos , Memoria/efectos de los fármacos , Enfermedad de Alzheimer/metabolismo , Enfermedad de Alzheimer/patología , Animales , Antioxidantes/administración & dosificación , Encéfalo/metabolismo , Encéfalo/patología , Modelos Animales de Enfermedad , Medicamentos Herbarios Chinos/administración & dosificación , Medicamentos Herbarios Chinos/farmacología , Flavanonas/administración & dosificación , Masculino , Malondialdehído/metabolismo , Aprendizaje por Laberinto/efectos de los fármacos , Estrés Oxidativo/efectos de los fármacos , Ratas , Ratas Sprague-Dawley , Rutaceae/química , Superóxido Dismutasa/metabolismo , Factores de Tiempo , Proteínas tau/metabolismoRESUMEN
In order to quantify the activity of heterotrophic microorganism in membrane bioreactor (MBR) for drinking water treatment, biomass respiration potential (BRP) test and 2,3,5-triphenyl tetrazolium chloride-dehydrogenase activity (TTC-DHA) test were introduced and modified. A sludge concentration ratio of 5:1, incubation time of 2h, an incubation temperature that was close to the real operational temperature, and using a mixture of main AOC components as the substrate were adopted as the optimum parameters for determination of DHA in drinking water MBR. A remarkable consistency among BDOC removal, BRP and DHA for assessing biological performance in different MBRs was achieved. Moreover, a significant correlation between the BRP and DHA results of different MBRs was obtained. However, the TTC-DHA test was expected to be inaccurate for quantifying the biomass activity in membrane adsorption bioreactor (MABR), while the BRP test turned out to be still feasible in that case.
Asunto(s)
Biomasa , Reactores Biológicos/microbiología , Purificación del Agua , Estudios de Factibilidad , Procesos Heterotróficos , Membranas Artificiales , Oxidorreductasas/metabolismo , Oxígeno/metabolismo , Aguas del Alcantarillado , Temperatura , Sales de Tetrazolio/metabolismoRESUMEN
OBJECTIVE: To investigate the protective effects of the total base from rhizoma coptis chinensis (CTB) and berberine (Ber) on neurodegeneration induced by aluminum overload in rats. METHODS: The study took place in the Department of Pharmacology, Chongqing Medical University, Chongqing, China, between February 2005 and May 2007. Wistar rats were divided into control group, model group, Ber-treated group, CTB (55 mg/kg and 110 mg/kg)-treated group, and nimodipine-treated group (n=20). A rat brain damage model was established via intragastric administration of 400 mg/kg element aluminum once a day, 5 days a week for 12 weeks. The CTB, Ber, and nimodipine were intragastrically administered 4 hours after each aluminum administration for 12 weeks. The morphological changes of the neurons of the rat hippocampus and the changes of rat learning and memory functions were observed. The superoxide dismutase (SOD), choline acetyltransferase (ChAT), acetylcholinesterase (AchE), and monoamine oxidase-B (MAO-B) activities and malondialdehyde (MDA) content, as well as the MAO-B expression in the rat brain were examined. RESULTS: The CTB, Ber, and nimodipine significantly improved the learning and memory ability impairment and hippocampal neuronal death. The CTB, Ber, and nimodipine also significantly blunted the decrease of SOD and ChAT activities, and the increase of MDA content, AchE activities, and MAO-B expressions and activity in the aluminum-overload rats. CONCLUSION: The CTB and Ber have protective effects on neurodegeneration induced by aluminum overload. The CTB (110 mg/kg) has more powerful neuroprotection than Ber.
Asunto(s)
Aluminio/toxicidad , Berberina/uso terapéutico , Lesiones Encefálicas/tratamiento farmacológico , Modelos Animales de Enfermedad , Medicamentos Herbarios Chinos , Enfermedades Neurodegenerativas/inducido químicamente , Animales , Masculino , Ratas , Ratas WistarRESUMEN
OBJECTIVE: To investigate the effect of small hairpin interfering RNA (shRNA) in suppressing cytochrome P450 3A4 (CYP3A4) gene expression in CHL-3A4 cells. METHODS: Three shRNA expression vectors targeting CYP3A4 gene (CYP3A4 I, C YP3A4 II, and CYP3A4 III, respectively) were designed, synthesized and transfected into CHL-3A4 cells via liposomes. The inhibitory effect of shRNA on CYP 3A4 gene expression was detected by Western blotting and RT-PCR, and the effect of shRNA transfection in suppressing cyclophosphamide-induced cytotoxicity was measured using MTT assay. RESULTS: The vector carrying CYP3A4 III shRNA significantly reduced the expression of CYP3A4 gene at both the mRNA (75%) and protein levels (80%) in CHL3A4 cells. The cytotoxicity of cyclophosphamide was markedly inhibited by CYP3A4 III-mediated suppression of CYP3A4 gene expression by 75% in CHL-3A4 cells. CONCLUSION: The vector-mediated RNA interference can suppress CYP3A4 gene expression in CHL-3A4 cells, and RNA interference technique provides a new means for studying cytochrome P450 gene function in mammalian cells.