RESUMEN
BACKGROUND: The potential risk of insecticidal proteins produced by genetically engineered (GE) plants to nontarget organisms have long been an ecotoxicological concern. Apanteles chilonis, an important endoparasitoid of rice pest Chilo suppressalis, potentially is exposed to Bacillus thuringiensis (Bt) endotoxins through a food chain of transgenic Bt rice - C. suppressalis - A. chilonis, and thus, a rigorous risk assessment is urgently needed. Here, we combined a tri-trophic bioassay system with high-dose exposure approach using C. suppressalis hemolymph as the carrier of insecticidal protein to evaluate the biosafety of Cry1Ca to A. chilonis. RESULTS: Cry1Ca protein could be transmitted and retained along the food chain and remains bioactive in the hemolymph of C. suppressalis during the pre-adult duration of A. chilonis. No significant differences in pre-adult period, male and female longevity, adult fecundity and weight, emergence rate nor sex ratio were observed when A. chilonis parasitized C. suppressalis feeding on cry1Ca rice compared with control treatment. However, the pupal period and weight were significantly prolonged and decreased. When A. chilonis parasitized C. suppressalis injected with a high dosage of Cry1Ca protein, no adverse effects on the life-history parameters, peroxidase (POD), superoxide dismutase (SOD) or glutathione reductase (GR) of A. chilonis were observed, demonstrating that the host quality mediates adverse effects during the food chain. CONCLUSIONS: We confirmed that Cry1Ca posed no ecological risk to the nontarget endoparasitoid A. chilonis. This study may serve as an example for future risk assessment of transgenic crops to nontarget endoparasitoids. © 2023 Society of Chemical Industry.
