Association between MTHFR C677T polymorphism and cognitive impairment in patients with cerebral small vessel disease: a cross-sectional study.

Objective: Cerebral small vessel disease (CSVD) is the most common vascular cause of cognitive impairment. This study aimed to explore the association between MTHFR C677T polymorphism and cognitive impairment in CSVD patients. Methods: Demographic, medical, laboratory, cognitive evaluation, and MTHFR C677T polymorphism data were collected from CSVD patients admitted to our hospital between January 2019 and July 2023. Inclusion criteria for CSVD were based on the Standards for Reporting Vascular changes on Neuroimaging (STRIVE) criteria, with age ≥ 45 years. Binary logistic regression models were used to analyze risk factors associated with WMH and cognitive impairment. Results: A total of 330 CSVD participants were recruited in this study, including 179 male and 151 female, with a median age of 64 years (interquartile range: 58-73 years). There were 185 patients (56.1%) with cognitive impairment, 236 patients (71.5%) with WMH, 89 patients (27.0%) with CMB, 87 patients (26.4%) with lacunes. All participants completed MTHFR polymorphism analysis, 149 cases (45.2%) of the CC genotype, 112 cases (33.9%) of the CT genotype and 69 cases (20.9%) of the TT genotype. Patients with TT genotype exhibited higher plasma homocysteine levels and more severe WMH and cognitive impairment (p < 0.001). Multivariable binary logistic regression model showed that WMH was significantly associated with age (p = 0.019), history of hypertension (p = 0.011), HHcy (p = 0.019) and MTHFR genotype (p = 0.041); while cognitive impairment was significantly associated with age (p = 0.033), history of hypertension (p = 0.019), HHcy (p = 0.040), MTHFR genotype (p = 0.039), WMH (p = 0.041), and lacunes (p = 0.001). Conclusion: In this cross-sectional study, we investigated the association between MTHFR C677T polymorphism and cognitive function in CSVD patients. We found that MTHFR 677 TT genotype was an independent risk factor for the progression of WMH and cognitive impairment in CSVD patients.

Clinical Analysis of Minimally Invasive Percutaneous Treatment of Severe Lumbar Disc Herniation with UBE Two-Channel Endoscopy and Foraminal Single-Channel Endoscopy Technique.

For surgical treatment, herniation of traditional open surgery is the traditional approach and the representative operation for lamina windowing excision of nucleus pulposus. In recent years, the unilateral dual channel spine endoscopic technology (UBE/BESS) has caused extensive concern of spine surgery performer. This research compared the results of minimally invasive percutaneous treatment of severe lumbar disc herniation with foraminal single-channel endoscopy and unilateral biportal endoscopy (UBE). A retrospective study was conducted on 50 patients with severe disc herniation treated with minimally invasive percutaneous treatment in MinDong Hospital affiliated to Fujian Medical University from September 2019 to September 2021. According to different surgical methods, they were divided into two groups: foraminal single-channel endoscopic group and UBE dual-channel endoscopic group. There were 22 cases in the UBE surgery group and 28 cases in the interforaminal endoscopic group. The comparison included operation time, postoperative hospital stays, preoperative and postoperative pain scale (VAS), and postoperative MRI to observe the residual condition of prolapsed nucleus pulposus and the occurrence of complications. There were no significant differences between the UBE group and the interforaminal endoscopic group in incision length, operation time, postoperative hospital stays, and improvement of VAS score before and after surgery. In terms of postoperative nucleus pulposus residual rate and postoperative recurrence rate, the two-channel UBE group was significantly better than the single-channel interforaminal endoscopic group. The incidence of postoperative anemia in the interforaminal endoscopic group was significantly lower than that in the UBE group. In the treatment of severe disc herniation, UBE two-channel endoscopy has the advantages of lower recurrence rate, lower nucleus pulposus residual rate, shorter learning curve, and better field of vision than foraminal single-channel endoscopy, which is worth promoting in primary hospitals.

Reconstructing Nanohydroxyapatite Prosthesis Based on CT-Scanning Data and Its Application in Spinal Injury.

This study investigated the nanohydroxyapatite (nHA) prosthesis application effect based on CT-scanning data in spinal injury. This study chose 26 spinal injury patients treated in our hospital from September 2017 to September 2018, who were randomly divided into two groups. nHA prosthesis based on CT-scanning data was implanted in the nHA group, whereas titanium mesh was implanted in the titanium mesh group. Consequently, osteoblasts were cultured to test the biological activity of nHA and titanium alloy. In cell tests, we found osteoblasts could better adhere to nHA, and proliferation and activity were higher when planted on nHA material. After surgical treatment, all patients' spinal symptoms (VAS score, JOA score, and Cobb angle) had improved and did not cause obvious inflammatory foreign body reactions. During a two-year follow-up, the fusion time and support settlement in the nHA group was lower, and the vertebral fusion rate and ASIA score were higher than those in the titanium mesh group. Thus, CT-scanning data could further improve the vertebral fusion rate in the nHA group. Consequentially, nHA prosthesis based on CT-scanning data is a better choice for spinal injury therapy.

LncRNA FLVCR1-AS1 accelerates osteosarcoma cells to proliferate, migrate and invade via activating wnt/ß-catenin pathway.

PURPOSE: To clarify the biological function of long non-coding RNA (lncRNA) FLVCR1-AS1 in the progression of osteosarcoma. METHODS: The correlation between FLVCR1-AS1 level and pathological indexes of osteosarcoma patients was analyzed by chi-square test. Subsequently, the regulatory effects of FLVCR1-AS1 on the proliferative, migratory and invasive abilities of osteosarcoma cells were evaluated. Moreover, the relative levels of CTNNB1, SOX4, CCND1, CCND2 and MYC in osteosarcoma cells regulated by FLVCR1-AS1 were detected by qRT-PCR. Finally, rescue experiments were conducted to verify the role of wnt/ß-catenin in osteosarcoma progression. RESULTS: LncRNA FLVCR1-AS1 was upregulated in osteosarcoma, which was positively correlated to tumor size, WHO grade and distant metastasis, but negatively correlated to survival of osteosarcoma patients. Overexpression of FLVCR1-AS1 markedly suppressed osteosarcoma cells to proliferate, migrate and invade. Relative levels of CTNNB1, SOX4, CCND1, CCND2, MYC and nucleus ß-catenin were upregulated in U2OS and MG63 cells overexpressing FLVCR1-AS1. Finally, CTNNB1 knockdown was identified to reverse the influence of overexpressed FLVCR1-AS1 of osteosarcoma cells. CONCLUSIONS: FLVCR1-AS1 accelerates the progression of osteosarcoma via activating wnt/ß-catenin pathway.