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1.
J Mater Chem B ; 12(28): 6856-6873, 2024 Jul 17.
Artículo en Inglés | MEDLINE | ID: mdl-38904349

RESUMEN

The management of chronic infected wounds poses significant challenges due to frequent bacterial infections, high concentrations of reactive oxygen species, abnormal immune regulation, and impaired angiogenesis. This study introduces a novel, microenvironment-responsive, dual dynamic, and covalently bonded hydrogel, termed OHA-P-TA/G/Mg2+. It is derived from the reaction of tannic acid (TA) with phenylboronic acids (PBA), which are grafted onto oxidized hyaluronic acid (OHA-P-TA), combined with GelMA (G) via a Schiff base and chemical bonds, along with the incorporation of Mg2+. This hydrogel exhibits pH and ROS dual-responsiveness, demonstrating effective antibacterial capacity, antioxidant ability, and the anti-inflammatory ability under distinct acidic and oxidative microenvironments. Furthermore, the release of Mg2+ from the TA-Mg2+ network (TA@Mg2+) promotes the transformation of pro-inflammatory M1 phenotype macrophages to anti-inflammatory M2 phenotype, showing a microenvironment-responsive response. Finally, in vivo results indicate that the OHA-P-TA/G/Mg2+ hydrogel enhances epithelial regeneration, collagen deposition, and neovascularization, showing great potential as an effective dressing for infected wound repair.


Asunto(s)
Hidrogeles , Magnesio , Taninos , Cicatrización de Heridas , Taninos/química , Taninos/farmacología , Cicatrización de Heridas/efectos de los fármacos , Hidrogeles/química , Hidrogeles/farmacología , Magnesio/química , Magnesio/farmacología , Animales , Ratones , Antibacterianos/química , Antibacterianos/farmacología , Pruebas de Sensibilidad Microbiana , Células RAW 264.7 , Staphylococcus aureus/efectos de los fármacos , Reactivos de Enlaces Cruzados/química , Polifenoles
2.
Biomacromolecules ; 25(4): 2486-2496, 2024 Apr 08.
Artículo en Inglés | MEDLINE | ID: mdl-38427705

RESUMEN

Multifunctional hydrogels with tissue adhesion, self-healing behavior, and antibacterial properties have potential in wound healing applications. However, their inefficient self-healing behavior and antibacterial agents can cause long-term cytotoxicity and drug resistance, considerably limiting their clinical use. Herein, we reported a PDA@LA hydrogel constructed by introducing polydopamine nanoparticles (PDA-NPs) into a high-density dynamic bonds cross-linked lipoic acid (LA) hydrogel that was formed by the polymerization of LA. Because of its rich carboxyl groups, the LA hydrogel could adhere firmly to various tissues. Owing to the high-density dynamic bonds, the cut LA hydrogel exhibited highly inefficient self-healing behavior and recovered to its uncut state after self-healing for 10 min. After the introduction of the PDA-NPs, the hydrogel was able to heat up to more than 40 °C to kill approximately 100% of the Escherichia coli and Staphylococcus aureus under near-infrared (NIR) laser, thus resisting wound infections. Because no toxic antibacterial agent was used, the PDA@LA hydrogel caused mild long-term cytotoxicity or drug resistance. Consequently, the adhesive, highly efficient self-healing, and NIR photothermal antibacterial PDA@LA hydrogel exhibits considerable potential for clinical use.


Asunto(s)
Hidrogeles , Ácido Tióctico , Humanos , Adherencias Tisulares , Hidrogeles/farmacología , Vendajes , Antibacterianos/farmacología , Escherichia coli , Cicatrización de Heridas
3.
Acta Biomater ; 179: 95-105, 2024 04 15.
Artículo en Inglés | MEDLINE | ID: mdl-38513723

RESUMEN

The osteoarthritic (OA) environment within articular cartilage poses significant challenges, resulting in chondrocyte dysfunction and cartilage matrix degradation. While intra-articular injections of anti-inflammatory drugs, biomaterials, or bioactive agents have demonstrated some effectiveness, they primarily provide temporary relief from OA pain without arresting OA progression. This study presents an injectable cartilage-coating composite, comprising hyaluronic acid and decellularized cartilage matrix integrated with specific linker polymers. It enhances the material retention, protection, and lubrication on the cartilage surface, thereby providing an effective physical barrier against inflammatory factors and reducing the friction and shear force associated with OA joint movement. Moreover, the composite gradually releases nutrients, nourishing OA chondrocytes, aiding in the recovery of cellular function, promoting cartilage-specific matrix production, and mitigating OA progression in a rat model. Overall, this injectable cartilage-coating composite offers promising potential as an effective cell-free treatment for OA. STATEMENT OF SIGNIFICANCE: Osteoarthritis (OA) in the articular cartilage leads to chondrocyte dysfunction and cartilage matrix degradation. This study introduces an intra-articular injectable composite material (HDC), composed of decellularized cartilage matrix (dECMs), hyaluronan (HA), and specially designed linker polymers to provide an effective cell-free OA treatment. The linker polymers bind HA and dECMs to form an integrated HDC structure with an enhanced degradation rate, potentially reducing the need for frequent injections and associated trauma. They also enable HDC to specifically coat the cartilage surface, forming a protective and lubricating layer that enhances long-term retention, acts as a barrier against inflammatory factors, and reduces joint movement friction. Furthermore, HDC nourishes OA chondrocytes through gradual nutrient release, aiding cellular function recovery, promoting cartilage-specific matrix production, and mitigating OA progression.


Asunto(s)
Cartílago Articular , Condrocitos , Osteoartritis , Ratas Sprague-Dawley , Animales , Condrocitos/efectos de los fármacos , Condrocitos/metabolismo , Condrocitos/patología , Osteoartritis/patología , Osteoartritis/tratamiento farmacológico , Osteoartritis/terapia , Cartílago Articular/patología , Cartílago Articular/efectos de los fármacos , Ratas , Ácido Hialurónico/química , Ácido Hialurónico/farmacología , Lubrificación , Masculino , Bovinos , Inyecciones Intraarticulares
4.
Int J Biol Macromol ; 261(Pt 1): 129679, 2024 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-38286381

RESUMEN

Early-stage esophageal cancer is primarily treated by endoscopic submucosal dissection (ESD). However, extensive mucosal dissection creates a significant risk of postoperative esophageal stricture. Clinically, postoperative stricture can be prevented by glucocorticoids; however, there are drawbacks to both systemic and local administration of glucocorticoids, and improving drug administration methods is crucial. In this study, we developed a chitosan-based thermosensitive hydrogel for triamcinolone (TA) delivery. Our results indicated that the hydrogel remains liquid at low temperatures and can be injected into the esophageal wound site through an endoscopic biopsy channel. Upon reaching body temperature, the hydrogel undergoes spontaneous gelation and firmly adheres to the wound surface. The liquid phase enables convenient and precise delivery, while the gel phase achieves remarkable adhesion, tensile strength, and resistance to degradation. Moreover, the hydrogel exhibited an extended release duration of >10 days when loaded with a 10 mg dose. In vitro studies revealed that the hydrogel suppresses the proliferation and fibrogenesis of human scar fibroblasts (HKF). In a rat skin dermal defect model, the hydrogel attenuated keloid formation during the healing process. Consequently, the chitosan-based thermosensitive hydrogel developed in this study for triamcinolone delivery may be an effective tool for preventing post-ESD esophageal stricture.


Asunto(s)
Quitosano , Resección Endoscópica de la Mucosa , Neoplasias Esofágicas , Estenosis Esofágica , Humanos , Animales , Ratas , Triamcinolona , Estenosis Esofágica/etiología , Estenosis Esofágica/prevención & control , Quitosano/farmacología , Resección Endoscópica de la Mucosa/efectos adversos , Resección Endoscópica de la Mucosa/métodos , Hidrogeles , Neoplasias Esofágicas/patología , Neoplasias Esofágicas/cirugía
5.
Carbohydr Polym ; 319: 121172, 2023 Nov 01.
Artículo en Inglés | MEDLINE | ID: mdl-37567713

RESUMEN

Accurate and controlled release of drug molecules is crucial for transdermal drug delivery. Electricity, as an adjustable parameter, offers the potential for precise and controllable drug delivery. However, challenges exist in selecting the appropriate drug carrier, electrical parameters, and release model to achieve controlled electronic drug release. To overcome these challenges, this study designed a functional hydrogel using polyvinyl alcohol, chitosan, and graphene oxide as components that can conduct electricity, and constructed a drug transdermal release model using fluorescein sodium salt with proper electrical parameters. The results demonstrated that the hydrogel system exhibited low cytotoxicity, good conductivity, and desirable drug delivery characteristics. The study also integrated the effects of drug release and tissue repair promotion under electrical stimulation. Cell growth was enhanced under low voltage direct current pulses, promoting cell migration and the release of VEGF and FGF. Furthermore, the permeability of fluorescein sodium salt in the hydrogel increased with direct current stimulation. These findings suggest that the carbohydrate polymers hydrogel could serve as a drug carrier for controlled release, and electrical stimulation offers new possibilities for functional drug delivery and transdermal therapy.


Asunto(s)
Quitosano , Grafito , Hidrogeles/farmacología , Alcohol Polivinílico , Preparaciones de Acción Retardada , Fluoresceína , Polivinilos , Sistemas de Liberación de Medicamentos , Electricidad , Portadores de Fármacos/toxicidad , Óxidos
6.
Int J Biol Macromol ; 250: 126177, 2023 Oct 01.
Artículo en Inglés | MEDLINE | ID: mdl-37558037

RESUMEN

Tracheoesophageal fistula (TEF) is an abnormal connection between the trachea and esophagus that severely impairs quality of life. Current treatment options have limitations, including conservative treatment, surgical repair, and esophageal stent implantation. Here, we introduced laponite (LP) nano-clay to improve chitosan-based hydrogels' rheological properties and mechanical properties and developed an endoscopically injectable nanocomposite shear-thinning hydrogel to seal and repair fistulas as an innovative material for the treatment of TEF. Excellent injectability, rheological properties, mechanical strength, self-healing, biodegradability, biocompatibility, and tissue repair characterize the new hydrogel. The introduction of LP nano-clay improves the gel kinetics problem of hydrogels to realize the sol-gel transition immediately after injection, avoiding gel flow to non-target sites. The addition of LA nano-clay can significantly improve the rheological properties and mechanical strength of hydrogels, and hydrogel with LP content of 3 % shows better comprehensive performance. The nanocomposite hydrogel also shows good cytocompatibility and can promote wound repair by promoting the migration of HEEC cells and the secretion of VEGF and FGF. These findings suggest that this nanocomposite hydrogel is a promising biomaterial for TEF treatment.


Asunto(s)
Quitosano , Fístula Traqueoesofágica , Humanos , Nanogeles , Calidad de Vida , Hidrogeles
7.
ACS Nano ; 17(14): 13358-13376, 2023 07 25.
Artículo en Inglés | MEDLINE | ID: mdl-37439514

RESUMEN

As chondrocytes from osteoarthritic cartilage usually exhibit aging and senescent characteristics, targeting aging chondrocytes could be a potential therapeutic strategy. In this study, exosomes derived from umbilical cord-derived mesenchymal stem cells (UCMSC-EXOs) combined with the chondrocyte-targeting capacity and controlled-release system were proposed for osteoarthritis (OA) treatment via rejuvenating aging chondrocytes. The essential functional miRNAs within UCMSC-EXOs were investigated, with the p53 signaling pathway identified as the key factor. To improve the therapeutic efficiency and retention time of UCMSC-EXOs in vivo, the exosomes (EXOs) were engineered on membranes with a designed chondrocyte-targeting polymers, and encapsulated within thiolated hyaluronic acid microgels to form a "two-phase" releasing system, which synergistically facilitated the repair of OA cartilage in a rat model. Together, this study highlighted the rejuvenating effects of UCMSC-EXOs on OA chondrocytes and the potential to combine with chondrocyte-targeting and sustained-release strategies toward a future cell-free OA treatment.


Asunto(s)
Exosomas , Células Madre Mesenquimatosas , MicroARNs , Osteoartritis , Ratas , Animales , Condrocitos/metabolismo , Exosomas/metabolismo , Preparaciones de Acción Retardada/metabolismo , Osteoartritis/terapia , MicroARNs/metabolismo , Células Madre Mesenquimatosas/metabolismo , Cordón Umbilical/metabolismo
8.
J Mater Chem B ; 11(23): 5207-5222, 2023 06 14.
Artículo en Inglés | MEDLINE | ID: mdl-37272389

RESUMEN

Ulcerative arterial bleeding is characterized by sudden onset, rapid disease development, and high mortality, which is a great challenge for clinicians to treat, specially bleeding in areas where endoscopic operation is difficult, or in the case of diffuse bleeding, tumor bleeding, and recurrent bleeding. Herein, we proposed a novel treatment strategy using biomaterials to protect the wound and isolate the erosion of digestive tract contents to prevent arterial bleeding in advance. By introducing chitosan to construct multihydrogen-bonding and an electrostatic interaction system, we developed polyethyleneimine/polyacrylic acid/chitosan (PEI/PAA/CS) multifunctional hydrogel. The new hydrogel is characterized by ultrafast gelation, strong tissue adhesion, gastric acid resistance, burst resistance, biocompatibility, hemostasis, and tissue repair. The addition of CS significantly improved the tissue adhesion, biocompatibility, hemostasis, and tissue repair ability of the hydrogel. The PEI/PAA/CS hydrogel could adhere to the ulcer surface and form a protective layer on the wound to prevent arterial bleeding. Importantly, the PEI/PAA/CS hydrogel also has the ability to stop bleeding and promote wound repair, which has been demonstrated in a variety of hemorrhage models in rats and rabbits. All of these factors indicate that the PEI/PAA/CS hydrogel is a promising biomaterial for reducing the risk of ulcerative arterial bleeding.


Asunto(s)
Quitosano , Ratas , Conejos , Animales , Quitosano/farmacología , Hidrogeles/farmacología , Polietileneimina , Adherencias Tisulares , Úlcera , Hemorragia/inducido químicamente , Hemorragia/tratamiento farmacológico , Materiales Biocompatibles/farmacología
9.
Small ; 19(40): e2302152, 2023 10.
Artículo en Inglés | MEDLINE | ID: mdl-37282789

RESUMEN

Cell migration is an essential bioactive ceramics property and critical for bone induction, clinical application, and mechanism research. Standardized cell migration detection methods have many limitations, including a lack of dynamic fluid circulation and the inability to simulate cell behavior in vivo. Microfluidic chip technology, which mimics the human microenvironment and provides controlled dynamic fluid cycling, has the potential to solve these questions and generate reliable models of cell migration in vitro. In this study, a microfluidic chip is reconstructed to integrate the bioactive ceramic into the microfluidic chip structure to constitute a ceramic microbridge microfluidic chip system. Migration differences in the chip system are measured. By combining conventional detection methods with new biotechnology to analyze the causes of cell migration differences, it is found that the concentration gradients of ions and proteins adsorbed on the microbridge materials are directly related to the occurrence of cell migration behavior, which is consistent with previous reports and demonstrates the effectiveness of the microfluidic chip model. This model provides in vivo environment simulation and controllability of input and output conditions superior to standardized cell migration detection methods. The microfluidic chip system provides a new approach to studying and evaluating bioactive ceramics.


Asunto(s)
Dispositivos Laboratorio en un Chip , Microfluídica , Humanos , Simulación por Computador , Movimiento Celular , Biotecnología
10.
Biomater Adv ; 146: 213286, 2023 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-36657218

RESUMEN

Endoscopic submucosal dissection (ESD) is a challenging procedure. The use of biomaterials to improve the operator's convenience (operating affinity) has received little attention. We prepared two thermosensitive hydrogels, lactobionic acid-modified chitosan/chitosan/ß-glycerophosphate thermosensitive hydrogel (hydrogel 1) and its lyophilized powders (hydrogel 2), characterized their physicochemical properties and evaluated their performance in ESD experiments on large animals, by comparing with the commonly used normal saline (NS) and glycerin fructose (GF). These hydrogels showed good low-temperature fluidity; their viscosities at 4 °C were 92.2 mPa.s and 26.9 mPa.s, respectively. The hydrogels provided significantly better viscoelastic properties than NS and GF. The relaxation moduli of hydrogels were higher than those of NS and GF when the strains were 1 %, 5 %, and 10 %. The hydrogels can be maintained for seven days, even at pH 1, after which they degrade entirely. In pig model experiments, we performed submucosal injection and ESD procedures in the stomach and esophagus. The cushion height produced by the hydrogels was higher than those of NS and GF 30 min after injection. The ESD operation time for hydrogels was significantly shorter. Postoperative wound observation and histological analysis showed that the hydrogels promoted wound healing. The two hydrogels differed in fluidity, viscoelasticity, and other properties, which makes it possible to select the hydrogels according to the size and location of the lesion during ESD operation, and hydrogel 2 may be more suitable for use in lengthier procedures. In general, the hydrogels showed good performance, facilitated the intraoperative operation of ESD, shorten the operation time and promoted wound healing, which is of great significance for reducing the complications and reducing the threshold of ESD operation and further promoting the popularity of ESD.


Asunto(s)
Quitosano , Resección Endoscópica de la Mucosa , Porcinos , Animales , Hidrogeles , Quitosano/química , Resección Endoscópica de la Mucosa/métodos , Estómago/cirugía , Materiales Biocompatibles , Glicerol
11.
Int J Biol Macromol ; 220: 109-116, 2022 Nov 01.
Artículo en Inglés | MEDLINE | ID: mdl-35970363

RESUMEN

Wound management requires the preparation of controllable, safe and effective dressings to isolate the wound from the external environment. Currently, widely used commercial dressings focus on the isolation effect rather than an environment conducive to wound healing. To provide the dressing with beneficial properties such as wetting and antioxidant and antibacterial activity, this study used polyvinyl alcohol (PVA) hydrogel as the base material and introduced chitosan (CS) and vanillin (V) to design a PVA/CS/V three-phase hydrogel dressing. The dressings were prepared using a freeze-thaw cycle to achieve properties. We conducted a comparative analysis of PVA/V and PVA/CS two-phase hydrogels. The PVA/CS/V (PCV) hydrogel dressing maintaining an elastic modulus at >5 kPa at 15-40 °C. An in vitro antibacterial assay showed the potent antibacterial ability of hydrogels against gram-positive and -negative bacteria, and cells in some PCV groups showed higher activity. The antioxidant results showed that PCV hydrogel had a potent scavenging effect on DPPH, ABTS+, and PTIO free radical. The antibacterial and antioxidant properties of three-phase hydrogel showed the best performance in all experimental groups. These results suggest that PCV hydrogel has value in commercial applications due to its simple preparation process and excellent biological properties.


Asunto(s)
Quitosano , Alcohol Polivinílico , Antibacterianos/química , Antibacterianos/farmacología , Antioxidantes/farmacología , Vendajes , Benzaldehídos , Quitosano/química , Hidrogeles/química , Alcohol Polivinílico/química
12.
J Biomater Appl ; 36(10): 1737-1747, 2022 05.
Artículo en Inglés | MEDLINE | ID: mdl-35244481

RESUMEN

Acute wounds are often contaminated by some kind of filth, and fluids are usually used to wash away the dirt, but the force of the fluid may cause secondary injury at the wound site or even increase the risk of infection. Hydrogels have several advantages over liquid scouring since they are less intense, more portable, and easier to control. In this study, poly(vinyl alcohol) was used to prepared a series of hydrogels, which were tested in terms of their properties and abilities to clean simulated dirty wounds. Simulated dirt and bacterial (Serratia marcescens) adhesion experiments demonstrated that they could effectively adhere to a certain amount of dirt or bacteria to achieve the purpose of wound cleaning. In addition to the bacterial adhesion, the antibacterial experiments also proved that the hydrogels have a certain inhibitory effect on the proliferation of E.coli and S.aureus. The hydrogels could change shape freely and exhibited excellent biocompatibility, ductility, and self-healing capabilities, which increase their service life and make them more suitable for treating wounds or acting as protection buffers. Based on all these characteristics, the developed hydrogel may be a potentially valuable material for wound cleaning.


Asunto(s)
Alcohol Polivinílico , Cicatrización de Heridas , Antibacterianos/farmacología , Hidrogeles/farmacología , Staphylococcus aureus
13.
Int J Biol Macromol ; 184: 593-603, 2021 Aug 01.
Artículo en Inglés | MEDLINE | ID: mdl-34174301

RESUMEN

The goal of this study was to develop intraoperative biomaterials for use in endoscopic submucosal dissection (ESD) procedures that are stable during storage, easy to use, and effective in clinical practice. Therefore, injectable thermosensitive hydrogels were developed based on lactobionic acid-modified chitosan/chitosan/ß-glycerophosphate (CSLA/CS/GP) hydrogel lyophilizate powders, and their properties were compared with original hydrogels that had not been freeze-dried. The results indicated that the lyophilizate powders retained their thermosensitive properties, and gels could be formed within 5 min at 37 °C. Compared to the original hydrogels, the injectability of the hydrogels derived from lyophilizate powders increased significantly. These novel materials maintained their original porous network lamellar structure but exhibited improved mechanical strength and tissue adhesion. Their application with L929 and GES-1 cells revealed that the lyophilizate powder hydrogels demonstrated good cytocompatibility and clearly protected the cells in an acidic environment. The results of submucosal injection experiments involving porcine stomach tissue indicated that the heights of the cushions created by CSLA/CS/GP lyophilizate powder hydrogels lasted longer than those generated with normal saline. The thermosensitive hydrogels based on lyophilizate powders may contribute to practical clinical applications involving ESD, and may also have potential value for other applications in the digestive tract.


Asunto(s)
Quitosano/administración & dosificación , Glicerofosfatos/química , Hidrogeles/síntesis química , Polvos/síntesis química , Animales , Línea Celular , Quitosano/química , Estabilidad de Medicamentos , Resección Endoscópica de la Mucosa , Liofilización , Humanos , Hidrogeles/química , Inyecciones , Ratones , Polvos/química , Porcinos , Termodinámica
14.
Carbohydr Polym ; 263: 118001, 2021 Jul 01.
Artículo en Inglés | MEDLINE | ID: mdl-33858584

RESUMEN

To develop a biomaterial to lift the lesion and promote wound healing in endoscopic submucosal dissection (ESD), we used lactobionic acid (LA) to improve the water solubility of chitosan (CS) and prepared a new three-phase hydrogel system with lactobionic acid-modified chitosan/chitosan/ß-glycerophosphate (CSLA/CS/GP). The results indicated that the hydrogel retains temperature-sensitive properties, and CSLA obviously improved the low-temperature fluidity of the hydrogel precursor solution, enabling injection of the hydrogel by endoscopic needle. The mechanical strength and bio-adhesion of the hydrogels were also improved by the addition of CSLA and the hydrogels could be maintained in acidic environment for a few days and exhibit greater protection of cells. The CSLA/CS/GP hydrogels show good cytocompatibility. The heights of cushions elevated by CSLA/CS/GP hydrogels remained ∼ 60 % 2 h post-injection in porcine stomach models. Given the unique characteristics of these materials, the CSLA/CS/GP thermo-sensitive hydrogel is a promising intraoperative biomaterial in ESD.


Asunto(s)
Quitosano/química , Disacáridos/química , Resección Endoscópica de la Mucosa/métodos , Hidrogeles/química , Animales , Materiales Biocompatibles/química , Línea Celular , Línea Celular Transformada , Proliferación Celular/efectos de los fármacos , Fibroblastos/efectos de los fármacos , Mucosa Gástrica/efectos de los fármacos , Humanos , Hidrogeles/síntesis química , Concentración de Iones de Hidrógeno , Fenómenos Mecánicos , Ratones , Sustancias Protectoras/química , Reología , Porcinos , Temperatura , Cicatrización de Heridas
15.
J Mater Chem B ; 8(34): 7659-7666, 2020 09 14.
Artículo en Inglés | MEDLINE | ID: mdl-32812629

RESUMEN

Increasing numbers of biodegradable medical devices may be used in the circulatory system. The effects of the released degradation products from these medical devices on the blood may be gradual and cumulative. When they reach critical levels, they may cause thrombosis and other complications. For this reason, it is important to evaluate the blood compatibility of degradation products for quality control and development of these devices. In the present study, we evaluated the degradation products of four biodegradable materials (collagen, polylactic acid, calcium phosphate ceramics, and magnesium) using platelet activation molecular markers that are associated with thrombosis. We found that the degradation products activate platelets to a certain extent, and that the degradation products produced during various degradation time periods activate platelets to varying degrees. This platelet activation occurs via several mechanisms, most of which are associated with the physicochemical properties of the degradation products, including ion concentration, pH, molecular microstructure, and molecular weight. Our findings not only provide a clearer understanding of the effects of degradation products from blood-contacting biodegradable devices, but also provide material for screening of degradation behavior so as to improve quality control for these devices.


Asunto(s)
Materiales Biocompatibles/efectos adversos , Materiales Biocompatibles/metabolismo , Activación Plaquetaria/efectos de los fármacos , Materiales Biocompatibles/química , Biomarcadores/metabolismo , Humanos , Concentración de Iones de Hidrógeno , Ensayo de Materiales , Peso Molecular , Trombosis/inducido químicamente , Trombosis/metabolismo , Trombosis/fisiopatología
16.
Thromb Res ; 185: 171-179, 2020 01.
Artículo en Inglés | MEDLINE | ID: mdl-31838449

RESUMEN

It is important to ascertain platelet responses to blood-contacting medical devices as part of a complete hemocompatibility evaluation. Nevertheless, researchers often face the problem of insufficient quantities of human blood for evaluation of platelet activation by actual medical devices. If animal blood can replace human blood to evaluate platelet activation by medical devices, testing will be smoother and will aid for quality control of related products. Therefore, in this study, we exposed representative biomaterials to human blood, rabbit blood and mouse blood, and evaluated similarities and differences in platelet activation among the three types of blood by measuring various molecular markers. We found that rabbit blood and human blood had considerable similarity in terms of platelet activation, while mouse blood and human blood showed considerable differences. Therefore, rabbit blood may replace human blood for platelet function testing.


Asunto(s)
Plaquetas , Activación Plaquetaria , Animales , Materiales Biocompatibles , Estudios de Factibilidad , Ensayo de Materiales , Conejos
17.
Colloids Surf B Biointerfaces ; 184: 110516, 2019 Dec 01.
Artículo en Inglés | MEDLINE | ID: mdl-31569002

RESUMEN

The effective assessment of platelet activation is an important component of the evaluation of cardiovascular implants. Currently, most evaluation is performed based on the ISO 10993-4 international standard. However, the methods specified in this standard were originally designed for non-degradable materials, and the applicability of these methods to evaluate degradable materials has not been carefully assessed. Here, the platelet activation response was evaluated (using blood from health rabbits) for three typical degradable materials (collagen, polylactic acid, and hydroxyapatite) by measuring the widely used molecular markers CD62 P, CD63, and CD40 L and the three molecular markers PF4, ß-TG, and TXB2 that are referenced in the ISO 10993-4 standard. The variations of these six markers were compared in the simulated degradation of the three test materials. The results showed differences in platelet activation with degradation that were strongly related to the surface physicochemical properties. Changes in the surface roughness and contact angle of the materials correlated with changes in the degree of platelet activation. The six tested platelet activation molecular markers show promise for assessment of platelet function in degradable medical devices, providing guidance for quality control strategies and the design and improvement of safe medical devices.


Asunto(s)
Materiales Biocompatibles/metabolismo , Plaquetas/metabolismo , Animales , Biomarcadores/metabolismo , Humanos , Tamaño de la Partícula , Activación Plaquetaria , Conejos , Propiedades de Superficie
18.
Mater Sci Eng C Mater Biol Appl ; 103: 109870, 2019 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-31349408

RESUMEN

Endoscopic submucosal dissection (ESD) is a useful tool for the treatment of early gastric neoplasms, however post-ESD bleeding and perforation restrict its widespread application. In this study, we developed the chitosan/ß-glycerophosphate/collagen (CS/GP/Col) thermo-sensitive systems to satisfy the requirements for the endoscopic treatment for ESD-induced ulcer. The results indicated that the addition of collagen to CS/GP system did not lead to remarkable changes on the physicochemical properties of the systems, which can transform from solution to hydrogels under physiological temperature within 90s, technically makes it suitable to be applied through catheter to gastric ulcer during ESD operation. Besides, hydrogels with high collagen concentration showed better biocompatibility, effectively protected L929, GES-1, HVSMC and CCD-18Co cells from acidic condition, induced more growth factors such as EGF, VEGF and FGF in those cells, and promoted coagulation. These results indicated that the CS/GP/Col thermo-sensitive hydrogel might be a promising biomaterial for the endoscopic treatment of ESD-induced ulcer, and further research can be carried out.


Asunto(s)
Quitosano , Colágeno , Mucosa Gástrica/cirugía , Gastroscopía/efectos adversos , Glicerofosfatos , Hidrogeles , Complicaciones Posoperatorias/tratamiento farmacológico , Neoplasias Gástricas/cirugía , Úlcera , Animales , Línea Celular , Quitosano/química , Quitosano/farmacología , Colágeno/química , Colágeno/farmacología , Mucosa Gástrica/patología , Glicerofosfatos/química , Glicerofosfatos/farmacología , Humanos , Hidrogeles/química , Hidrogeles/farmacología , Ratones , Ratas , Ratas Sprague-Dawley , Neoplasias Gástricas/patología , Úlcera/tratamiento farmacológico , Úlcera/etiología
19.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi ; 36(2): 232-237, 2019 Apr 25.
Artículo en Chino | MEDLINE | ID: mdl-31016939

RESUMEN

New biodegradable intravascular stent can reduce risk of foreign bodies retained, thus, it is widely concerned and some of the products have been introduced into the clinic. However, the characteristic of biodegradable may lead to more safety concerns associated with thrombosis. To ensure the safety, the thrombus formation experiment in vivo needs to be carefully designed and evaluated based on GB/T 16886.4 standard, but current standard do not provide explicit testing and evaluating methods. Establishing animal model with experimental pigs, the study compares biodegradable coronary stents and metal stents by simulating clinical implantation in vivo on the thrombus formation in the implanting process, and after the short-term and long-term implantation. The evaluation methods include gross observation, digital subtraction angiography intraoperative analysis, optical coherence tomography analysis, scanning electron microscopy and so on. The results show that combining these methods could comprehensively evaluate the whole process of the thrombus formation from the beginning of implantation to the end of preclinical animal experiments, so that, it may better predict the clinical thrombosis risk, and the selection of the control was very important. The study tries to use the comparison examples of thrombosis on the new medical instrument to provide the clue for thrombosis evaluation in vivo on similar instruments and show the methodology on the preclinical evaluation.


Asunto(s)
Implantes Absorbibles/efectos adversos , Stents Liberadores de Fármacos/efectos adversos , Trombosis/etiología , Animales , Polímeros , Porcinos , Tomografía de Coherencia Óptica , Resultado del Tratamiento
20.
Carbohydr Polym ; 209: 198-206, 2019 Apr 01.
Artículo en Inglés | MEDLINE | ID: mdl-30732799

RESUMEN

In order to prepare a submucosal injection agent that can gel during the endoscopic mucosal dissection (ESD) and still adhere to the wound surface after the surgery to reduce intraoperative and postoperative complications, a thermosensitive hydrogel based on chitosan/ß-glycerophosphate (CS-GP) was constructed. Hydroxypropylcellulose (HPC) and collagen Ⅰ were added into the hydrogel to improve the adhesion ability, viscosity and biocompatibility. The effect of HPC on the viscosity, strength and bio-adhesion and collagen Ⅰ on the biocompatibility and promoting on the wound restoration were intensively studied. The results indicated that HPC and collagen Ⅰ will not obviously change pH of the system and the system was still physically cross-linked. HPC increased the initial gelation time but the time of the entire gelation process is shortened, especially the high-concentration HPC, and improved the mechanical strength obviously. The viscosity and the bio-adhesion of the CS-GP hydrogels were improved by the addition of HPC. Also, the mixed collagen Ⅰ offered good cytocompatibility. According to the results of cultured GES-1 cell, it can be seen that the addition of HPC and collagen Ⅰ does not inhibit cell proliferation, and shows more obvious protection on the cells under acidic conditions. All of these suggested that the application of this hydrogel were more promising in ESD surgery than which without HPC and collagen Ⅰ.


Asunto(s)
Materiales Biocompatibles/química , Celulosa/análogos & derivados , Quitosano/química , Resección Endoscópica de la Mucosa , Hidrogeles/química , Temperatura , Adhesividad , Materiales Biocompatibles/farmacología , Proliferación Celular/efectos de los fármacos , Celulosa/química , Celulosa/farmacología , Colágeno Tipo I/química , Diseño de Fármacos , Humanos , Concentración de Iones de Hidrógeno , Reología , Viscosidad
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