The role of TRIM25 in the occurrence and development of cancers and inflammatory diseases.

The tripartite motif (TRIM) family proteins are a group of proteins involved in different signaling pathways. The changes in the expression regulation, function, and signaling of this protein family are associated with the occurrence and progression of a wide range of disorders. Given the importance of these proteins in pathogenesis, they can be considered as potential therapeutic targets for many diseases. TRIM25, as an E3-ubiquitin ligase, is involved in the development of various diseases and cellular mechanisms, including antiviral innate immunity and cell proliferation. The clinical studies conducted on restricting the function of this protein have reached promising results that can be further evaluated in the future. Here, we review the regulation of TRIM25 and its function in different diseases and signaling pathways, especially the retinoic acid-inducible gene-I (RIG-I) signaling which prompts many kinds of cancers and inflammatory disorders.

Development and evaluation of bioactive 3D zein and zein/nano-hydroxyapatite scaffolds for bone tissue engineering application.

The aim of this study is to generate and investigate biodegradable and biocompatible zein and zein/nano-hydroxyapatite composite scaffolds for bone defect healing. 3D zein scaffold was successfully fabricated using the salt-leaching method and incorporated with 12.5 wt% nHA for osteogenic differentiation of murine myoblast cell line (C2C12 cells). The scaffolds were subjected to physicochemical and biomechanical characterizations using the scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), biodegradation, porosity, mechanical tests. C2C12 cells were cultured on scaffolds and incubated for 21 days. Cell proliferation was detected by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Quantitative real-time PCR was used to test the expression of osteoblastic-related genes including Runx2, ALP, and Col1A1. The scaffolds had an adequate mean pore size and a total porosity of 61.1%-70.6%. The addition of 12.5 wt% nHA to the zein scaffold increased the compressive modulus to 79.1 MPa and the ultimate strength to 2.7 MPa. The qRT-PCR analysis confirmed that mRNA transcript levels were significantly higher (p < 0.05) on the zein/nHA than on the pure zein scaffold. The results suggested that the developed scaffolds could be a potential candidate for bone tissue engineering due to their promising osteoinductivity, surface topography, mechanical behavior, biodegradability.