RESUMEN
The D- - phenotype is a genetic variant of the Rh blood group system. It expresses D antigen but lacks C, c, E and e antigens. In D- - phenotype, the RHCE coding region is extensively modified by RHD sequence replacement, nucleotide deletion or splice-site changes. This article reports the identification of a new D- - haplotype in a Comorian man. It exhibits a hybrid gene in which RHCE gene exons 3-8 have been replaced by RHD sequences on the RHCE * C allele background. This allele is associated with no expression of c/C and e/E antigens and overexpression of RhD antigen.
Asunto(s)
Sistema del Grupo Sanguíneo Rh-Hr/genética , Antígenos de Grupos Sanguíneos/genética , Antígenos de Grupos Sanguíneos/inmunología , Comoras , Epítopos/genética , Epítopos/inmunología , Haplotipos , Humanos , Masculino , Fenotipo , Sistema del Grupo Sanguíneo Rh-Hr/inmunologíaRESUMEN
BACKGROUND: The purpose of the study was to analyze serologic and molecular markers of the GB virus type C/hepatitis G virus (GBV-C/HGV) infection in voluntary blood donors from Southeastern France. STUDY DESIGN AND METHODS: Sera were tested for the presence of GBV-C/HGV RNA by reverse transcriptase-polymerase chain reaction and that of antibodies to the GBV-C/HGV E2 (anti-E2) antigen by an enzyme-linked immunosorbent assay. A first cohort (1660 blood donors) was tested prospectively and a second cohort (238 samples with hepatitis markers) was tested retrospectively. Donors in the prospective study were questioned for possible risk factors of virus transmission. Amplification products were sequenced and subjected to phylogenetic analysis. RESULTS: Approximately 2.6 percent of individuals accepted for blood donation and 15.4 percent with positive hepatitis C virus serologic tests carried GBV-C/HGV RNA. Anti-E2 was detected in these two populations in approximately 12 percent and 48 percent of donors, respectively. Moderate relative risks were found only in tattooed or pierced individuals (1.82) and health care workers (2.45). Almost all strains were located in the same phylogenetic branch as HGV Group 2. CONCLUSION: Though a large proportion of the donors tested have been in contact with GBV-C/HGV, no elevated relative risk of infection was identified. The phylogenetic distribution of viral strains suggests that the infection is endemic in this population.
Asunto(s)
Flaviviridae/genética , Hepatitis Viral Humana/epidemiología , Adolescente , Adulto , Alanina Transaminasa/sangre , Biomarcadores/sangre , Donantes de Sangre , Estudios de Cohortes , Femenino , Francia , Genoma Viral , Hepatitis Viral Humana/sangre , Humanos , Masculino , Reacción en Cadena de la Polimerasa , Prevalencia , Estudios Prospectivos , ARN/análisis , Reacción a la Transfusión , Regiones no TraducidasRESUMEN
During the course of suramin-induced differentiation, a marked lysosomal storage disorder is observed in HT29-D4. This impairment could account for the toxic side effects of the drug during clinical trials in humans. It is shown that the perturbation is caused by a process of endocytosis of suramin-serum albumin complexes by the apical membrane of differentiated HT29-D4 cells.