Advances in Integrated Antimicrobial Resistance Surveillance and Control Strategies in Asia-Pacific Economic Cooperation Economies: Assessment of a Multiyear Building Capacity Project.

Antimicrobial resistance (AMR) is a growing global health concern for both animal and public health, and collaborative strategies are needed to combat the threat. The level of awareness and funding for policies focused on reducing AMR varies between countries. The aim of this study was to compare the integrated surveillance systems for AMR in high and low-middle economies of the Asia-Pacific Economic Cooperation and determine whether there was any improvement from 2015 to 2018. We conducted a survey with a group of 21 countries at different development levels. Associations between the economic development level and the questions of AMR awareness and funding were established using Fisher's exact test. Improvements were identified where countries established public policies for integrated surveillance of AMR. High economies showed greater advancement in several topics related to AMR than low-middle economies. The survey revealed that there is a better understanding surrounding the implications of the emergence of AMR in human medicine than in veterinary medicine, agriculture, and food production. Our results show that countries enhanced overall AMR surveillance over the 4-year-period; however, more research is needed concerning these advances, especially in low-middle economies and the food production sector.

Determination of five antimicrobial families in droppings of therapeutically treated broiler chicken by high-performance liquid chromatography-tandem mass spectrometry.

Antimicrobials are currently used in poultry for disease treatment. However, their excretion in bird feces may contaminate the environment. Considering this, the objective of this work was to quantify antimicrobials residues concentrations in therapeutically treated broiler chicken droppings throughout the post-treatment period. For this aim a multiresidue method using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was validated. Forty-eight male broiler chickens were distributed and treated with commercial formulations of 5 different antimicrobials. Results showed that oxytetracycline and 4-epi-oxytetracycline, presented the highest concentrations during all sampling period, detecting concentrations of 1471.41 µg kg-1 at the last sampling point (day 22 post-treatment). Florfenicol, tylosin, enrofloxacin, and ciprofloxacin were eliminated and detected in treated chicken droppings until d 18 post-treatment. Sulfachloropyridazine decrease gradually during post-treatment period until day 30. Results demonstrate that studied antimicrobials in treated chicken droppings were eliminated for prolonged periods, therefore becoming a significant route of residues dissemination into the environment.

Phenotypic and Genotypic Characterization of Virulence Factors and Susceptibility to Antibiotics in Salmonella Infantis Strains Isolated from Chicken Meat: First Findings in Chile.

Salmonella Infantis is a zoonotic pathogen that causes gastroenteritis in humans and animals, with poultry being its main reservoir. In Chile, there are no data to characterize S. Infantis strains in poultry production. In this study, 87 S. Infantis strains were isolated from chicken meat for sale in supermarkets in Santiago, Chile, and characterized according to their virulence genes, biofilm formation abilities, antibiotic susceptibility, and resistance genes. Through polymerase chain reaction or PCR, the strains were analyzed to detect the presence of 11 virulence genes, 12 antibiotic resistance genes, and integrase genes. Moreover, disc diffusion susceptibility to 18 antimicrobials and the ability to form biofilm in vitro were evaluated. Results demonstrated six different virulence gene profiles. Ninety-four percent of the strains were multi-resistant to antibiotics with weak biofilm formation abilities, 63.2% of the strains were broad spectrum ß- lactam resistant, and the bla CTX-M-65 gene was amplified in 13 strains. Only 3.4% of the strains were fluoroquinolone resistant, and the qnrB gene was amplified in two strains. Colistin resistance was exhibited in 28.7% of the strains, but mrc genes were not amplified in any strain under study. The isolated S. Infantis strains are pathogenic and antibiotic multi-resistant, and thus, this Salmonella serotype should be under surveillance in the poultry food production chain with the aim of protecting public health.

Estructura lingüística y Teoría de la Mente en trastorno del lenguaje / Language form and the theory of mind in language impairment

El concepto de Teoría de la Mente (ToM, de su sigla en inglés) hace referencia a la capacidad de ponerse en el lugar de la otra persona y ser así capaz de interpretar creencias, deseos e intenciones de otros. El propósito de esta investigación fue determinar el tipo de relación entre la adquisición de la forma del lenguaje y el desarrollo de la Teoría de la Mente en niños con Trastorno Específico del Lenguaje TEL tipo mixto y en niños con desarrollo típico, debido a que la literatura en relación con el tema es escasa y se focaliza principalmente en niños con autismo. La investigación se llevó a cabo mediante una metodología de tipo cuantitativa, un diseño no experimental-transversal y un alcance descriptivo y correlacional. Los instrumentos utilizados para evaluar el desarrollo de la estructura del lenguaje fueron el Test de Procesos de Simplificación Fonológica TEPROSIF-R y el Test de Comprensión Auditiva de E-Carrow TECAL; en tanto, para determinar el desarrollo de la ToM se utilizaron la prueba de Sally y Anne y la prueba de Mentalización. La muestra fue no probabilística y estuvo constituida por 28 niños con TEL mixto (grupo estudio) y 28 niños con desarrollo normal (grupo control) de 5 a 6 años, 11 meses. Los resultados indicaron que existe una relación entre el desarrollo de la teoría de la mente y el desarrollo de la estructura del lenguaje en su modalidad expresiva y comprensiva, principalmente en los niños con TEL mixto. A partir de estos resultados se puede concluir que es necesario intervenir desde el quehacer fonoaudiológico, además de las habilidades lingüísticas, aquellas habilidades asociadas con la Teoría de la Mente.

The concept of Theory of Mind ToM refers to the ability to put oneself in the other person's place and be able to interpret beliefs, desires and intentions of others. The purpose of this research was to determine the relationship between the development of the language form and the ToM in children with mixed Specific Language Impairment (SLI) and children with typical language development because the literature on the topic is limited and it focuses mainly on children with autism. The research was carried out using a quantitative methodology, a non-experimental-cross-sectional design, and a descriptive and correlational scope. The instrument used to determinate the development of the language structure were Test de Procesos de Simplificación Fonológica (TEPROSIF-R) and Test de Comprensión Auditiva de E-Carrow (TECAL); meanwhile, to evaluate the development of the ToM were used Sally and Anne Task and Mentalization test. The sample consisted on 28 children with SLI (study group) and 28 children with normal language development (control group) from 5 to 6,11 years old. The results indicate that there is a relationship between the development of the ToM and the development of the structure of language in its expressive and comprehensive modality, mainly in children with Mixed SLI. From these results, it is concluded that it is necessary to intervene not only linguistic abilities but also those abilities related to ToM from the speech therapist tasks.