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1.
Sci Rep ; 14(1): 5711, 2024 03 08.
Artículo en Inglés | MEDLINE | ID: mdl-38459201

RESUMEN

This study aimed to investigate the biological activities of Lactobacillus gasseri SM 05 (L. gasseri) and Lacticaseibacillus casei subsp. casei PTCC 1608 (L. casei) in the black raspberry (Rubus dolichocarpus) juice (BRJ) environment, and also the anti-adhesion activity against Salmonella typhimurium (S. typhimurium) in fermented black raspberry juice (FBRJ). Results showed significant anti-adhesion activity in Caco-2 epithelial cells. In the anti-adhesion process, lactic acid bacteria (LAB) improve intestinal health by preventing the adhesion of pathogens. Adding LAB to BRJ produces metabolites with bacteriocin properties. Major findings of this research include improved intestinal health, improved antidiabetic properties, inhibition of degradation of amino acids, and increase in the nutritional value of foods that have been subjected to heat processing by preventing Maillard inhibition, and inhibition of oxidation of foodstuff by increased antioxidant activity of BRJ. Both species of Lactobacillus effectively controlled the growth of S. typhimurium during BRJ fermentation. Moreover, in all tests, as well as Maillard's and α-amylase inhibition, L. gasseri was more effective than L. casei. The phenolic and flavonoid compounds increased significantly after fermentation by both LAB (p < 0.05). Adding Stevia extract to FBRJ and performing the HHP process showed convenient protection of phenolic compounds compared to heat processing.


Asunto(s)
Lacticaseibacillus casei , Lactobacillus gasseri , Probióticos , Rubus , Stevia , Humanos , Fermentación , Células CACO-2 , Extractos Vegetales/farmacología
2.
Probiotics Antimicrob Proteins ; 16(2): 413-425, 2024 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-36928935

RESUMEN

This study aimed to optimize the processing of probiotic yogurt supplemented with cumin essential oil (CEO), vitamin C, D3 (Vit D), and reduction of fermentation time using response surface methodology as a new functional food for diabetics with desirable sensory properties. The central composite design (CCD) was used to analyze the effect of these independent variables on the growth of the Lactobacillus plantarum A7 (LA7), starter culture, and overall acceptability. Differences between treatments were analyzed. The data were evaluated by analysis of variance at the significance level of 0.05. The effective concentration of CEO and fermentation time had the significant effect on the Lactobacillus plantarum A7 (LA7) number. Variance analysis and three-dimensional graphs show that almost the only effective factor on the overall acceptability of probiotic yogurt containing essential oil and vitamin D3 was CEO. According to the obtained data from the analysis, the optimal amount of independent variables for probiotic yogurt formulation such as CEO, D3, and fermentation time was 0.02% (v/v), 400 IU, and 9 h, respectively. This functional product can be considered an efficient food to reduce or eliminate the complications of diabetes.


Asunto(s)
Diabetes Mellitus , Lactobacillus plantarum , Aceites Volátiles , Probióticos , Yogur/análisis , Alimentos Funcionales , Fermentación
3.
BMC Microbiol ; 23(1): 126, 2023 05 10.
Artículo en Inglés | MEDLINE | ID: mdl-37165317

RESUMEN

BACKGROUND: Campylobacter regarded as a major cause of foodborne gastroenteritis in humans. The present study aimed to determine the prevalence of campylobacter in food, animal and human samples of Iran. RESULTS: Quantitative synthesis was performed from 119 articles. White meat had the highest pooled prevalence of Campylobacter spp. (43.9%). Pooled prevalence of 7.9% and 5.5% for Campylobacter, respectively, were determined for red meat and eggs from Iran. Campylobacter was seen in 14.9% of environmental samples and 8.4% of human samples. In most of the samples C. jejuni had higher frequency than C. coli. Most of the isolated Campylobacter harbored several of the known virulence related genes of this pathogen. CONCLUSION: Chicken was identified as the Campylobacter reservoir. As such preventive strategies in all stages of poultry production until consumption are necessary to control foodborne human infection with Campylobacter in Iran.


Asunto(s)
Infecciones por Campylobacter , Campylobacter jejuni , Campylobacter , Animales , Humanos , Campylobacter/genética , Campylobacter jejuni/genética , Prevalencia , Irán/epidemiología , Carne , Microbiología de Alimentos , Infecciones por Campylobacter/epidemiología , Infecciones por Campylobacter/veterinaria , Pollos
4.
Environ Sci Pollut Res Int ; 30(14): 40942-40951, 2023 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-36626050

RESUMEN

This research is aimed at the analysis of 87 pesticides in 30 fresh pistachio samples prepared from stores in Iran by QuEChERS-ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). The results showed at least one pesticide was in 67% of fresh pistachio samples. Kresoxim methyl residue was detected in 20 samples with average of 0.11 mg kg-1; this average is 2.2 times more than maximum residue limit (MRL). Buprofezin was recognized in five samples with average of 0.17 mg kg-1 was observed with 3.4 times more than MRL; hexaconazole and permethrin were recognized in three samples lower than MRL with an average residue of 0.030 and 0.028 mg kg-1, respectively. In addition, potential non-carcinogenic and carcinogenic health risk assessments were evaluated using probabilistic methods performed with the Monte Carlo simulation algorithm. The order of pesticide ranking based on hazard quotient (HQ) was hexaconazole > buprofezin > permethrin > kresoxim methyl. Total HQ (HI) was 2.0E-4 and for children, 9.0E-4. Hence, it means fresh pistachio consuming maybe not have significant short-term health risks for consumer. Ranking based on cancer risk (CR) was hexaconazole > kresoxim methyl > metalaxyl > permethrin > buprofezin. However, total CR due to pesticide residues was not higher than 1E-6 value (1.09E-9); therefore, consumers were not at significant risk of carcinogenicity in this product.


Asunto(s)
Residuos de Plaguicidas , Pistacia , Niño , Humanos , Espectrometría de Masas en Tándem/métodos , Residuos de Plaguicidas/análisis , Carcinógenos/análisis , Permetrina/análisis , Irán , Método de Montecarlo , Medición de Riesgo , Contaminación de Alimentos/análisis
5.
Heliyon ; 9(1): e12937, 2023 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-36711301

RESUMEN

Given that iron-deficiency anemia is a major nutritional problem in Iran and bread is one of the main foods in Iranian household basket, a flour fortification program with iron was established in 2001. Thereafter, to quality control of the mentioned program, the iron concentration was measured in flour samples. Accordingly, this study was conducted to investigate the iron content in wheat flour samples in Tehran, Iran. One hundred and twenty-one samples of wheat flour (i.e., Confectionery, Taftoon, Setareh, Barbary, Sangak wheat flour) were randomly collected from bakeries in Tehran by simple random sampling method. The content of iron was determined by flame atomic absorption spectrometry. The mean levels of iron in Confectionery, Taftoon, Setareh, Barbary, Sangak flour were 18.56 ± 5.64 ppm, 28.32 ± 1.74 ppm, 17.21 ± 5.02 ppm, 32.81 ± 3.98 ppm, 14.02 ± 4.99 ppm, respectively. The mean iron concentration of all sample groups was not complied with the minimum recommended level set by the Iranian Ministry of Health and Medical education (40 ppm). The mean iron content of all tested flour was significantly (P ≤ 0.05) lower than the minimum recommended level. The highest iron level was obtained in Barbary (32.81 ppm) and Taftoon (28.32 ppm) flour. While the lowest mean iron level was obtained in Sangak flour (14.02 ppm), followed by Setareh (17.21 ppm), and Confectionery (18.56 ppm) flour. In conclusion, it was identified that the iron fortification program in Tehran, Iran was not well performed as the minimum required level of iron in wheat flours (40 ppm) was not fulfilled. Therefore, the supervision and encouragement of the authorities to provide iron-fortified flours is highly recommended.

6.
BMC Public Health ; 22(1): 1856, 2022 10 04.
Artículo en Inglés | MEDLINE | ID: mdl-36195900

RESUMEN

BACKGROUND: Creating an environment for emotional and social well-being is an important responsibility of Health-Promoting and Child-Friendly Schools. Thus, the present study aimed to assess cultural adaptation and validation of the Persian version of the Psycho-Social Environment (PSE) Profile. The second purpose of this study was to survey the psycho-social environment of schools among a local sample of Iranian school staff. METHODS: This study was conducted in two phases, including cultural adaptation and validation of a culturally adapted scale. The cultural adaptation process followed the procedure suggested by Beaton et al. Then, the culturally adapted scale was administered to a local sample of Iranian school staff including managers (21.9%), teachers (57.4%), support staff (4%), and other school staff (16.7%) in a cross-sectional study. The participants' mean age was 39.98 ± 8.11 years and they were mostly female (62.8%). The psychometric properties of the culturally adapted version of the questionnaire were tested using a confirmatory factor analysis (n = 265), and a test of internal consistency. Finally, the status of schools' psycho-social environment was assessed using descriptive and analytical statistics. RESULTS: Confirmatory factor analysis indicated an overall good fit for the 7-factor profile (χ2/df: 1.906, PNFI: 0.62, TLI: 0.78, CFI: 0.79, RMSE: 0.059). The test of internal consistency showed an acceptable reliability (α = 0. 98). CONCLUSIONS: The Persian version of the PSE profile was culturally adapted for use in Iranian schools. Certainly, this culturally adapted version of PSE profile could be useful to determine the school psycho-social environment and to make any changes that can promote a friendly school climate for all participants, and to enhance learning and development.


Asunto(s)
Instituciones Académicas , Adulto , Comparación Transcultural , Estudios Transversales , Femenino , Humanos , Irán , Masculino , Persona de Mediana Edad , Psicometría/métodos , Reproducibilidad de los Resultados , Encuestas y Cuestionarios
7.
BMC Public Health ; 22(1): 1148, 2022 06 08.
Artículo en Inglés | MEDLINE | ID: mdl-35676671

RESUMEN

AIM: The objective of this study was to develop a cultural adopted questionnaire for evaluation of knowledge (K), attitude (A) and practice (P) of Iranian population toward food safety during Covid-19. METHODS: The study is based on an online questionnaire that filled by 712 Iranians over 16 years old. Exploratory factor analysis (EFA), confirmatory factor analysis (CFA) and reliability assessment were performed. The construct validity of A and P determined by EFA and confirmed by CFA. Difficulty index was used for K. RESULTS: The reliability score of questionnaire was satisfactory. The three items of K-A-P questionnaire were significantly associated with the total score of questionnaire. The KAP questionnaire regarding food safety in covid-19 consisted of 27 items multidimensional scale with strong psychometric features. The respondent showed a satisfactory level of KAP during covid-19 pandemics. CONCLUSION: The KAP questionnaire regarding food safety in covid-19 is a valid and reliable tool for measurement of knowledge, attitude and practice of people regarding food safety in covid-19.


Asunto(s)
COVID-19 , Adolescente , COVID-19/epidemiología , Inocuidad de los Alimentos , Conocimientos, Actitudes y Práctica en Salud , Humanos , Higiene , Irán/epidemiología , Pandemias/prevención & control , Psicometría/métodos , Reproducibilidad de los Resultados , Encuestas y Cuestionarios
8.
J Diabetes Metab Disord ; 21(1): 475-481, 2022 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-35673492

RESUMEN

Background: With increasing life expectancy and the aging population of most countries, attention to the diseases of old age has also increased. Due to the high prevalence of vitamin D deficiency in the elderly, the present study was designed and performed to investigate the relationship between serum vitamin D levels in Iranian elderly with the risk of metabolic syndrome (MetS). Methods: This study was conducted on an elderly population of Birjand (60 and older) in the urban and rural areas of the city. In 1325 elderly men and women, with age range of 60 and older, serum 25-hydroxyvitamin D, anthropometric measurements and laboratory variables were measured. Results: Twenty-eight percent of the elderly participants in the study had vitamin D deficiency. We found that participants with higher 25(OH)D had lower odds for elevated waist circumference (WC) (OR = 0.91, 95% CI 0.83-0.98), reduced HDL (OR = 0.96, 95% CI 0.86-0.97) and elevated fasting blood sugar (FBS) (OR = 0.96, 95% CI 0.89-0.98). We not found any significant association between 25(OH)D levels and other MetS components. Conclusion: Higher 25(OH)D levels in the elderly are linked to a reduced prevalence of MetS and, specifically, higher levels of favorable HDL-C, WC and FBS. Further intervention studies are needed to substantiate the results of this study.

9.
Trop Anim Health Prod ; 54(1): 62, 2022 Jan 17.
Artículo en Inglés | MEDLINE | ID: mdl-35037143

RESUMEN

Brucellosis is a zoonotic infectious disease with a worldwide distribution. This cross-sectional study aimed to determine the occurrence of Brucella in milk and blood samples of ruminants and the importance factors associated with animal brucellosis in Eastern Iran. A total of 200 paired samples, including blood (100) and milk (100), were obtained from the goats, sheep, and cows in Eastern Iran. Serum agglutination (SAT) and 2-mercapto ethanol (2-ME) tests were performed on the sera. A multiplex-polymerase chain reaction (m-PCR) assay was performed to identify the following species of Brucella, including B. abortus biovar 1, 2, 4, 3b, 5, 6, and 9, B. abortus S19, B. melitensis, and B. melitensis Rev.1 vaccine strain. B. abortus RB51 vaccine strain was also investigated in a second PCR assay. Risk factors for infection with Brucella spp. including the effect of abortion, contact with the wild animals, herd type, age, and previous vaccination in predicting blood contamination with B. abortus biovar 1, 2, and 4 were modeled by use of the artificial neural network. A total of 23 samples were seropositive regarding SAT and 2-ME tests. In total, B. abortus was detected in 35% and 15% of blood and milk samples, respectively, by the m-PCR assay. One sample of each of milk and blood was detected to have B. melitensis. Some samples were simultaneously contaminated with two Brucella species or two biovars of B. abortus. B. abortus S19 and B. melitensis Rev.1 vaccine strains were also detected in milk and blood samples. The sensitivity and specificity of the ANN model were 81% and 62%, respectively. In conclusion, B. abortus had higher frequency than B. melitensis in blood and milk samples. ANN determined herd type, previous vaccination, and age of the animal as the largest predictors of blood contamination with B. abortus.


Asunto(s)
Brucella melitensis , Brucelosis , Enfermedades de los Bovinos , Enfermedades de las Cabras , Enfermedades de las Ovejas/epidemiología , Animales , Brucella abortus/genética , Brucella melitensis/genética , Brucelosis/epidemiología , Brucelosis/veterinaria , Bovinos , Enfermedades de los Bovinos/epidemiología , Estudios Transversales , Femenino , Enfermedades de las Cabras/epidemiología , Cabras , Irán/epidemiología , Reacción en Cadena de la Polimerasa Multiplex/veterinaria , Redes Neurales de la Computación , Embarazo , Factores de Riesgo , Ovinos
10.
Int J Environ Health Res ; 32(11): 2396-2405, 2022 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-34406901

RESUMEN

This study aimed to investigate microbial, chemical, and heavy metal contamination of mechanically deboned chicken (MDC) in Iran. A total of 24 samples of MDC were obtained from meat plants. TBC of the three samples were acceptable. E. coli and S. aureus were detected in 21 and 6 samples, respectively. Three of the samples were contaminated with Salmonella spp. Campylobacter was not detected in any of the samples. The moisture content of MDC was in the range of 41% to 75%. Ash had a range of 0.74% to 1.4%. The maximum protein content of the MDC was 21.98% and fat content was in the range of 2.1% to 20%. The highest PV was 15.18 mEq/kg. All of the samples were polluted with Pb, Cd, and As. In conclusion, MDC had microbial and chemical contamination. It is necessary to develop more strict criteria for control of the chicken paste processing method.


Asunto(s)
Pollos , Microbiología de Alimentos , Animales , Cadmio , Recuento de Colonia Microbiana , Escherichia coli , Contaminación de Alimentos/análisis , Irán , Plomo , Carne , Staphylococcus aureus
11.
Int J Food Sci ; 2021: 3009795, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-34957294

RESUMEN

BACKGROUND: Regarding the increasing public health concerns about the safety of foodstuffs, the current survey was designed to argue the presence of preservatives (e.g., sodium benzoate (SB), potassium sorbate (PS), and natamycin) and also the level of salt and fungi in 148 samples of yoghurt drink "Doogh." METHODS: The enumeration of fungi and determination of salt content of samples were performed according to the standard procedures. Preservative determination was performed by reverse phase high-performance liquid chromatography with UV detection (RV-HPLC-UV). RESULTS: 0.1% of the total analyzed samples was above the permitted level of Iranian standard for SB (0%), while PS was not detected in any of them. Furthermore, natamycin in 0.11% of the analyzed samples had more than the permissible level of Iranian standard. Additionally, about 15% of the tested samples was higher than the Iranian standard level for fungi (<102 CFU/mL). The average amount of salt in the tested Doogh samples and also in the examined Kefir samples was significantly (P < 0.001) lower than the standard amount of salt (<0.8 g/100). CONCLUSION: In conclusion, the quality of Doogh and Kefir samples were acceptable in terms of salt content. Kefir had a significantly (P ≤ 0.001) lower amount of salt in comparison with Doogh. Taken together, underlining the results of the present study, no significant public health concern would exist respecting the mentioned additives.

12.
BMC Geriatr ; 21(1): 612, 2021 10 30.
Artículo en Inglés | MEDLINE | ID: mdl-34717587

RESUMEN

BACKGROUND: Few data are available on the nutritional status of Iranian geriatric population. The present study aimed to determine the nutritional status of older adults' population in Birjand, East of Iran. METHODS: The community-based cohort study was performed on older adults population (60 years and older) living in urban and rural areas of Birjand. The mini nutritional assessment (MNA) questionnaire was used to assess nutritional status. Anthropometric and biochemical evaluation were also performed for all of the participants. RESULTS: A total of 1417 geriatric person were enrolled in this study, which, most of them were female (51.9 %). According to MNA tool, most of the participants (73.3 %) had normal nutrition (MNA score ≥ 24). Malnourished (MNA score < 17) and at high risk of malnutrition (MNA score: 17- 23.5) were constituted 0.9 % and 25.8 % of the participants, respectively. Marital status, occupation, period of education and family member were associated with nutritional status. Multiple logistic regression showed that with increase of body mass index (BMI) (OR = 0.96), education years (OR = 0.95), hemoglobin (OR = 0.86) and lymphocyte (OR = 0.98), the odds of malnutrition decreased, but with increase of age the odds (1.03) of malnutrition is also elevated. CONCLUSIONS: MNA could successfully forecast the risk of malnutrition and malnourished people. Sociodemographic factors are associated with the nutritional status.


Asunto(s)
Evaluación Geriátrica , Evaluación Nutricional , Anciano , Estudios de Cohortes , Estudios Transversales , Femenino , Humanos , Irán/epidemiología
13.
Int J Microbiol ; 2021: 1499869, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-34512763

RESUMEN

The prevalence of Yersinia enterocolitica in gastroenteritis is often underestimated. It relates considerably to morbidity and medical expenses around the world. Understanding the cause of gastroenteritis leads to making the appropriate treatment decisions. We systematically searched PubMed, Science Direct, Embase, and Scopus to identify all published studies between Jan. 1, 2000, and Dec. 31, 2019, to assess the prevalence of Y. enterocolitica in gastroenteritis patients. A total of 5039 articles were identified that lead to the extraction of data from 47 of them. The pooled prevalence of Y. enterocolitica in cases of gastroenteritis was estimated as 1.97% (1.32-2.74%) in the culture method and 2.41% (1.07-4.22%) in the molecular method. Among the biotypes of Y. enterocolitica, 1A (62.48%) and 1B (2.14%) had the most and least prevalence, respectively. Serotype O3 Y. enterocolitica with 39.46% had the highest and O5,27 with 0.0% had the least prevalence in gastroenteritis cases. In conclusion, the findings of this systematic review show that Y. enterocolitica is prevalent in gastroenteritis in all age groups. Serotypes O3 and O9 of Y. enterocolitica had the highest prevalence and O5,27 had the least prevalence in diarrheal patients. The prevalence of Y. enterocolitica was similar in both gender and different seasons. It should be noted that to determine the role of the organism, more studies are needed especially in food-borne diseases.

14.
Environ Monit Assess ; 193(8): 540, 2021 Jul 31.
Artículo en Inglés | MEDLINE | ID: mdl-34331145

RESUMEN

The polycyclic aromatic hydrocarbon (PAH) content of the Tahdig of the breads and potatoes prepared with edible oil was determined by GC-MS. The Tahdigs of bread and potato were baked under the same condition (volume of any oil 40 cc, temperature 180 °C, time 30 min). Polycyclic aromatic hydrocarbon determination was performed by an Agilent 6890 N Gas chromatography with mass selective detector, equipped with a capillary column. The highest contents of PAHs in Tahdig of bread and Tahdig of potato were observed in canola with 550 ± 3.9 ng/kg and sesame with 408.3 ± 41 ng/kg. The mean of PAH content was significantly higher in the Tahdig of bread compared to the Tahdig of potato (p < 0.05). Among the 16 PAHs examined by GC/MS, 10 PAHs were detected. The amount of high molecular weight (HMW) PAHs were significantly more than low molecular weight (LMW) PAHs (p < 0.05). Benzo [b]fluoranthene and benz[a]anthracene concentrations were significantly more than the other detected compounds (p < 0.05). Due to high PAH contamination of both Tahdig groups, the consumption of Tahdig (any type) was not recommended.


Asunto(s)
Hidrocarburos Policíclicos Aromáticos , Solanum tuberosum , Pan , Monitoreo del Ambiente , Cromatografía de Gases y Espectrometría de Masas , Hidrocarburos Policíclicos Aromáticos/análisis
15.
Toxicol Rep ; 8: 53-59, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-33409123

RESUMEN

The present study aimed to determine the average concentration of some metals, including cadmium (Cd), chromium (Cr), copper (Cu), nickel (Ni) and lead (Pb) in the chicken, hen's liver, and gizzard in the east of Iran. Estimated daily intake (EDI), target hazard quotient (THQ), hazard index (HI) and carcinogenic risk (CR) were calculated. In this cross-sectional study, fifty one samples including chicken, hen's liver and gizzard were obtained from Birjand, Iran. Measurement of Cd, Cr, Cu, Ni, and Pb was carried out by using an Inductively Coupled Plasma-Optic Emission Spectroscopy (ICP-OES). All of the measured metals were detected in 100 % of the samples. The metals had a different distribution pattern. The highest concentration of Cd and Cu was in the liver samples while the Cr and Ni had the highest levels in the chicken. Pb concentration was at the highest level in the gizzard. The least amount of Cr, Ni, and Pb was found in the liver while Cu had the least content in the muscle. EDI had an acceptable level, but the highest daily intake of all studied metals was through muscle. Cr had the highest THQ and it was more than one in the meat. HI in chicken was more than one. Liver and gizzard of hens had a neglectable HI. CR was neglectable in the case of both Cd and Pb, but it was considerable for Cr and Ni. The consumption of chicken in both adults and children may pose a significant health risk for consumers.

16.
Nutr J ; 19(1): 56, 2020 06 16.
Artículo en Inglés | MEDLINE | ID: mdl-32546160

RESUMEN

BACKGROUND: The objectives of this study are to construct a cultural adopted questionnaire for evaluation of consumer's attitudes toward functional foods among Iranian's population according to Theory of Planned Behavior (TPB) and to investigate the attitudes toward vitamin enriched foods (VEF). METHODS: Participants were students of Birjand University of Medical Sciences (BUMS). Exploratory factor analysis (EFA), Confirmatory Factor Analysis (CFA) and reliability assessment were performed. The construct validity of questionnaire determined by EFA and confirmed by CFA. RESULTS: The overall Cronbach's alpha of questionnaire was 0.78. The three domains of TPB model were significantly associated with the total score of attitude toward functional foods questionnaire (AFFQ). Attitudes and subjective norms could successfully predict the consumption of VEF (p < 0.023 and p < 0.001, respectively), but perceived control construct could not perform the prediction (p < 0.219). CONCLUSION: AFFQ is a valid and reliable instrument to measure the attitudes of consumers toward consumption of functional foods in Iran.


Asunto(s)
Actitud , Alimentos Funcionales , Humanos , Irán , Reproducibilidad de los Resultados , Encuestas y Cuestionarios
17.
Oman Med J ; 35(2): e107, 2020 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-32181009

RESUMEN

OBJECTIVES: Dental units are necessary tools for modern dentistry. Microbial contamination of dental units is thought to be the result of biofilm formation in various parts of the unit. We aimed to identify the total microbial count in the suction hose of dental units, and detect the presence of Pseudomonas aeruginosa. METHODS: Random sampling of suction hoses of dental units in Birjand, Iran, was performed in dental clinics on the first (n = 115) and last (n = 115) working days of the week. Total viable counts of bacteria and detection of P. aeruginosa were performed on plate count agar and cetrimide agar, respectively. Plates were incubated at 37 °C for 48 hours. RESULTS: None of the samples were free from bacterial contamination. P. aeruginosa was detected in 22.6% and 18.3% of samples taken on the first and last working days, respectively. CONCLUSIONS: Suction hoses were heavily contaminated with bacteria, especially opportunistic pathogens, and current disinfection does not adequately control the contamination.

18.
Microb Drug Resist ; 26(4): 412-419, 2020 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-31618135

RESUMEN

Multidrug resistance of pathogenic microorganisms is a common problem in the treatment of infections. Therefore, searching for new agents with antimicrobial activities appears to be essential. Thrombocidin-1 (TC-1) is an antimicrobial peptide (AMP) derived from platelets. The present study aimed to produce recombinant TC-1 (rTC-1) with His tag to evaluate its antimicrobial activity and also predict its thermal-stability through molecular dynamic (MD) simulations. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration of rTC-1 against bacterial isolates were determined. Considering the importance of thermal-stability of proteins in their therapeutic applications, thermal-stability of rTC-1 predicted through MD simulation during 25ns at two important temperatures including avian normal body temperature and water boiling temperature at sea level. MIC results revealed that rTC1 had the most and least potency against Salmonella enteritidis and Escherichia coli, respectively. The root-mean-square deviation of rTC-1 during 25ns MD simulations revealed that this protein is stable at avian normal body temperature (40°C) but might lose stability at water boiling temperature at sea level. rTC-1 as an AMP has a good potency against some bacterial pathogens especially Salmonella spp. and E. coli 0157:H7. rTC-1 can be used as an alternative for common antimicrobial agents used in clinical settings.


Asunto(s)
Antibacterianos/farmacología , Farmacorresistencia Bacteriana Múltiple/efectos de los fármacos , Escherichia coli/efectos de los fármacos , Proteínas Citotóxicas Formadoras de Poros/farmacología , Salmonella enteritidis/efectos de los fármacos , Animales , Aves/microbiología , Temperatura Corporal/efectos de los fármacos , Pruebas de Sensibilidad Microbiana/métodos
19.
BMC Res Notes ; 12(1): 703, 2019 Oct 28.
Artículo en Inglés | MEDLINE | ID: mdl-31661017

RESUMEN

OBJECTIVE: This study aims to evaluate the total bacterial and fungal count of tools, devices and surfaces of kitchens of the centers affiliated to Birjand University of Medical Sciences. In this study, 200 samples from four kitchens of Birjand University of Medical Sciences were obtained. After the preparation of serial dilutions, samples were cultured in plate count agar (PCA) plates and Sabouraud dextrose agar (SDA). After incubation at 37 and 25 °C for 24-48 and 72-96 h respectively, the microbial and fungal colonies were counted. RESULTS: The mean bacterial and fungal count of kitchens was 7.7 * 107 and 7.6 * 104, respectively. The highest and lowest levels of bacterial contamination were related to tools/devices and cover of tools/work clothes and the highest and lowest levels of fungal count were related to forks and spoons and the tools and devices of the storage site. The rate of contamination in the kitchens of Birjand University of Medical Sciences was relatively high. Thus, serious, continuous and accurate monitoring of the units, training of people working in all stages of cooking and disinfection the tools and devices are essential for control and prevention of disease transmission.


Asunto(s)
Bacterias/aislamiento & purificación , Contaminación de Equipos , Hongos/aislamiento & purificación , Recuento de Colonia Microbiana , Culinaria , Universidades
20.
Environ Sci Pollut Res Int ; 26(30): 30980-30987, 2019 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-31452120

RESUMEN

Organochlorine pesticides (OCPs) are a more persistent and lipophilic group of insecticides. The present study aims to evaluate the residues of OCPs in meat, liver, and kidney of the cattle and sheep. A total of 54 samples were obtained from butcheries and local markets in Birjand, east of Iran. The residual contents of aldrin; dieldrin; dichlorodiphenyltrichloroethane; and its metabolites (DDTs), endosulfan isomers, endrin, hexachlorocyclohexane isomers (HCHs), heptachlor, heptachlor epoxide, chlordans, methoxychlor, and hexachlorobenzene (HCB), were analyzed in all samples. OCP residues were extracted by using the QuEChERS technique and quantified by gas chromatography-mass spectrometry (GC-MS). All of the analyzed OCPs were lower than the detection limit. The findings showed that meat and edible organs marketed in this region enjoyed a good status in terms of the residues of OCP. Monitoring of pesticide residues in meat and edible organs is necessary from the public health point of view.


Asunto(s)
Contaminación de Alimentos/análisis , Hidrocarburos Clorados/análisis , Carne/análisis , Residuos de Plaguicidas/análisis , Animales , Bovinos , Análisis de los Alimentos , Irán , Riñón/química , Límite de Detección , Hígado/química , Ovinos
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