Evaluation of canine and feline leukocyte differential counts obtained with the scil vCell 5 compared to the Advia 2120 hematology analyzer and a manual method.

The vCell 5 (scil Animal Care), a point-of-care hematology analyzer (POCA), was recently introduced to veterinary laboratories. This laser- and impedance-based analyzer is capable of providing a CBC with 5-part WBC differential count (Diff) along with WBC cytograms and flags serving as interpretation aids for numerical results. We compared the scil POCA-Diff to reference methods (i.e., manual differential count, Advia 2120 hematology analyzer [Siemens]) for canine and feline blood samples and considered WBC cytograms and flags. Total observed error (TEo), calculated from CV and bias%, was compared to total allowable error (TEa). Data were analyzed before and after a review process (exclusion of flagged and samples with invalid cytograms). For both species, correlation was good-to-excellent (rs = 0.81-0.97) between both analyzers for all variables, except for feline monocytes (rs = 0.21-0.63) and canine monocyte% (rs = 0.50). Smallest biases were seen for neutrophils (dog: -5.7 to 0.8%; cat: 1.5-9.4%) with both reference methods. Quality requirements (TEo < TEa) were fulfilled for canine and feline neutrophils (TEo = 5.3-10.6%, TEa = 15%) and eosinophils (TEo = 67.1-83%, TEa = (90)-50%) considering at least one reference method. Our review process led to mildly higher rs-values for most variables. Although not completely satisfactory, the scil POCA provides reliable results in compliance with ASVCP quality goals for canine and feline neutrophils and eosinophils. Analyzer flag and cytogram analysis served as useful tools for QA, indicating the necessity for manual review of blood smears, and contributed to improvement of scil POCA performance.

Evaluation of the scil vCell 5, a novel laser- and impedance-based point-of-care hematology analyzer, for use in dogs and cats.

A novel laser- and impedance-based point-of-care hematology analyzer (POCA), the vCell 5 (scil Animal Care), providing a complete blood count with 5-part leukocyte differential count has recently been introduced to veterinary laboratories. We evaluated the analyzer for use in dogs and cats including method comparison and assessment of linearity, carryover, and precision. Fresh blood samples from 192 healthy and diseased dogs and 159 cats were analyzed, and results were compared to reference methods (i.e., microhematocrit [PCV], Advia 2120 hematology analyzer). Total observed error (TEo) was calculated from CV, obtained at 3 concentrations, and bias%, and compared to total allowable error (TEa). For both species, excellent correlation (rs = 0.93-0.99) was seen between methods for WBC and RBC, hematocrit, hemoglobin, and platelet counts (PLT), except for feline PLT (rs = 0.79). Quality requirements (TEo < TEa) were fulfilled for WBC (TEo = 8.6-11.1%; TEa = 20%) and RBC (TEo = 3.5-7%; TEa = 10%), hematocrit (TEo = 5.7-9.4%; TEa = 10%), PCV (cat TEo = 7.8%; TEa = 10%), mean corpuscular volume (cat TEo = 5.1%; TEa = 7%), and PLT (TEo = 13.1-24.1%; TEa = 25%). Excellent linearity was demonstrated for WBC, RBC, and PLT, and hemoglobin. CVs of <2% for WBC, RBC, hematocrit, hemoglobin, and of <5% (dog) and 8% (cat) for PLT were demonstrated for values within the RI. Except for calculated variables and well-known species-specific deviations in feline PLT, scil POCA results were correlated favorably with reference method results and complied with quality requirements for cats and dogs.