RESUMEN
Anthocyanins have been reported to have effective chemopreventive activity. Lycium ruthenicum Murray is rich in anthocyanins and exhibits many biological activities. The purpose of this study was to investigate the effects and possible biological mechanism of the main anthocyanin monomer (Pt3G) of Lycium ruthenicum Murray on prostate cancer DU-145 cells. The cell proliferation was detected by methyl thiazolyl tetrazolium assay. The cell apoptosis rates were assessed by flow cytometric analysis and TUNEL assay. The expressions of apoptosis related proteins were evaluated by western blotting. Our data demonstrated that Pt3G inhibited cell proliferation, induced apoptosis and promoted cell cycle arrest at the S phase in a concentration-dependent manner (0, 100, 200 and 400 µg mL-1). Furthermore, it was shown that Pt3G decreased the mitochondrial membrane permeability through regulating the expressions of Bax and Bcl-2. Western blot analysis indicated that Pt3G significantly increased the expression of PTEN and then activated the PI3K/Akt-mediated caspase 3 pathway. In addition, our results also suggested that Pt3G activated the PTEN gene to induce the apoptosis of DU-145 cells by stimulating the overproduction of ROS. To sum up, these results indicate that Pt3G inhibits cell proliferation and induces apoptosis through the ROS/PTEN/PI3K/Akt/caspase 3 signaling pathway in prostate cancer DU-145 cells. Therefore, Pt3G of Lycium ruthenicum Murray may be a potential anti-proliferative agent for the prevention or treatment of prostate cancer.
Asunto(s)
Antocianinas/farmacología , Apoptosis/efectos de los fármacos , Glucósidos/farmacología , Lycium/química , Fosfatidilinositol 3-Quinasas/metabolismo , Neoplasias de la Próstata/tratamiento farmacológico , Antocianinas/aislamiento & purificación , Antineoplásicos Fitogénicos/farmacología , Caspasa 3/metabolismo , Línea Celular Tumoral , Proliferación Celular/efectos de los fármacos , Frutas/química , Glucósidos/aislamiento & purificación , Humanos , Masculino , Potencial de la Membrana Mitocondrial/efectos de los fármacos , Fosfohidrolasa PTEN/metabolismo , Neoplasias de la Próstata/metabolismo , Neoplasias de la Próstata/prevención & control , Proteínas Proto-Oncogénicas c-akt/metabolismo , Especies Reactivas de Oxígeno/metabolismo , Transducción de Señal/efectos de los fármacosRESUMEN
OBJECTIVE: To study the preliminary characterizations of Hyriopsis cumingii proteoglycan (HCPG). METHODS: The content of carbohydrate and protein were measured by spectrophotometry. FTIR spectrum was used to analyze the functional groups. Relative molecular mass and amino acid composition were detected by HPLC. GC was utilized to determine the monosaccharide composition. The glycopeptide linkage-bond was detected by using the method of beta-elimination reaction. RESULTS: In HCPG,the content of carbohydrate and protein was 80.06% and 9.42%, respectively. FTIR spectrum showed the characteristic absorptions of polysaccharides and protein. Relative molecular mass of HCPG, determined by size-exclusive HPLC, was 503.1 kDa. GC spectra demonstrated that polysaccharide of HCPG was composed of rhamnose, fucose, mannose, glucose and galactose with a molar ratio of 13.80: 4.51: 7.70: 64.92 : 9.07. Fourteen amino acids (13 known and one unknown) have been detected by pre-column derivation HPLC. From beta-elimination reaction, peptide chain was attached to the carbohydrate chain by O-glycosidic bond. CONCLUSION: Basic characterizations of HCPG have been determined preliminarily.
