Application of visual intelligent labels in the assessment of meat freshness.

With the increasing demand for meat products, the evaluation and real-time monitoring of its freshness has become one of the focuses of related industry research. Conventional freshness detection methods, including sensory evaluation, microbial experiments, and determination of physicochemical indicators, are time-consuming, low sensitivity, and destructive, so there is an urgent need to develop a convenient, intuitive, and inexpensive detection method. As a representative of smart packaging, visual intelligent labels can realize real-time perception and monitoring of meat freshness by measuring the temperature, pH value or other indicators of meat and converting them into visual signals. This paper first summarizes the common types, basic principles and research progress of visual intelligent labels, then introduces its application in livestock, poultry and seafood freshness monitoring, finally looks forward to the development prospect of visual smart labels.

Conservation opportunities through improved management of recently established protected areas in Southeast Asia.

Protected areas (PAs) play a crucial role in biodiversity conservation and climate change mitigation.1,2 However, ineffective management can lead to biodiversity loss and carbon emissions from deforestation.3,4,5,6 To address this issue and explore viable solutions, we assessed the impact of PA establishment on avoided deforestation in 80 Southeast Asian PAs using the synthetic control approach.7,8 Our results show that 36 PAs successfully prevented 78,910 ha of deforestation. However, the remaining 44 PAs lost 72,497 ha of forest, impacting the habitat of 226 threatened bird and mammal species. Effective management of these reserves could have potentially avoided up to 2.07 MtCO2e yr-1 in carbon emissions. We estimate that at least $17 million USD per year in additional funding is required to better manage these 44 ineffective PAs and reduce future emissions. Furthermore, we demonstrate that carbon markets have the potential to generate these funds by reducing carbon emissions from deforestation within protected areas. Our findings emphasize that improving PA management is an essential nature-based solution for conserving biodiversity and mitigating climate change.

The neglected role of abandoned cropland in supporting both food security and climate change mitigation.

Despite the looming land scarcity for agriculture, cropland abandonment is widespread globally. Abandoned cropland can be reused to support food security and climate change mitigation. Here, we investigate the potentials and trade-offs of using global abandoned cropland for recultivation and restoring forests by natural regrowth, with spatially-explicit modelling and scenario analysis. We identify 101 Mha of abandoned cropland between 1992 and 2020, with a capability of concurrently delivering 29 to 363 Peta-calories yr-1 of food production potential and 290 to 1,066 MtCO2 yr-1 of net climate change mitigation potential, depending on land-use suitability and land allocation strategies. We also show that applying spatial prioritization is key to maximizing the achievable potentials of abandoned cropland and demonstrate other possible approaches to further increase these potentials. Our findings offer timely insights into the potentials of abandoned cropland and can inform sustainable land management to buttress food security and climate goals.

Gaps and weaknesses in the global protected area network for safeguarding at-risk species.

Protected areas are essential to biodiversity conservation. Creating new parks can protect larger populations and more species, yet strengthening existing parks, particularly those vulnerable to harmful human activities, is a critical but underappreciated step for safeguarding at-risk species. Here, we model the area of habitat that terrestrial mammals, amphibians, and birds have within park networks and their vulnerability to current downgrading, downsizing, or degazettement events and future land-use change. We find that roughly 70% of species analyzed have scant representation in parks, or occur within parks that are affected by shifts in formal legal protections or are vulnerable to increased human pressures. Our results also show that expanding and strengthening park networks across just 1% of the world's land area could preserve irreplaceable habitats of 1191 species that are particularly vulnerable to extinction.

Climate co-benefits of tiger conservation.

Biodiversity conservation is increasingly being recognized as an important co-benefit in climate change mitigation programmes that use nature-based climate solutions. However, the climate co-benefits of biodiversity conservation interventions, such as habitat protection and restoration, remain understudied. Here we estimate the forest carbon storage co-benefits of a national policy intervention for tiger (Panthera tigris) conservation in India. We used a synthetic control approach to model avoided forest loss and associated carbon emissions reductions in protected areas that underwent enhanced protection for tiger conservation. Over a third of the analysed reserves showed significant but mixed effects, where 24% of all reserves successfully reduced the rate of deforestation and the remaining 9% reported higher-than-expected forest loss. The policy had a net positive benefit with over 5,802 hectares of averted forest loss, corresponding to avoided emissions of 1.08 ± 0.51 MtCO2 equivalent between 2007 and 2020. This translated to US$92.55 ± 43.56 million in ecosystem services from the avoided social cost of emissions and potential revenue of US$6.24 ± 2.94 million in carbon offsets. Our findings offer an approach to quantitatively track the carbon sequestration co-benefits of a species conservation strategy and thus help align the objectives of climate action and biodiversity conservation.

Hydrochromic Perovskite System with Reversible Blue-Green Color for Advanced Anti-Counterfeiting.

The intrinsic instability of halide perovskites toward to external stimulus, has created a competitive advantage for designing stimuli-responsive materials. However, the external environment tuning reversibly fluorescence emission of perovskite system is still limited. In this work, humidity is verified to act as a new option to modulate the emission properties of mixed-halide perovskite. The perovskite nanocrystals (PNCs) photoirradiated in dichloromethane are easily and stably redispersed in water, and emit bright fluorescence which is quite different from the original. Moreover, the perovskites confined on glass slide can reversibly switch their fluorescence between blue and green colors under moisture. It is demonstrated that the factors of different solubilities of CsCl and CsBr in water, the structural transformation of perovskites and the confine of glass matrix play key roles in the reversible transformation. Finally, the combination of hydrochromic CsPb(Brx Cly )3 and water-resistant CsPb(Brx Cly )3 -polymethyl methacrylate have been applied in advanced anti-counterfeiting, which greatly improves the information security. This work not only give an insight into the effects of humidity on fluorescence and structures of PNCs, but also offer a new class of hydrochromic PNCs materials based on reversible emission transformation for potential application in sensors, anti-counterfeiting and information encryption.

Quaternion attention multi-scale widening network for endoscopy image super-resolution.

Objective. In the field of endoscopic imaging, Super-Resolution (SR) plays an important role in Manufactured Diagnosis, physicians and machine Automatic Diagnosis. Although many recent studies have been performed, by using deep convolutional neural networks on endoscopic SR, most of the methods have large parameters, which limits their practical application. In addition, almost all of these methods treat each channel equally based on the real-valued domain, without considering the difference among the different channels. Our objective is to design a SR model named Quaternion Attention Multi-scale Widening Network (QAMWN) for endoscopy images to address the above problem.Approach. QAMWN contains a stacked Quaternion Attention Multi-Scale Widening Block, that composed of Multi-scale Feature Widening Aggregation Module (MFWAM) and Quaternion Residual Channel Attention (QRCA). The MFWAM adopts multi-scale architecture with step-wise widening on feature channels for better feature extraction; and in QRCA, quaternion is introduced to construct Residual Channel Attention Mechanism, which obtains adaptively scales features by considering compact cross-channel interactions in the hyper-complex domain.Main results. To verify the efficacy of our method, it is performed on two public endoscopic datasets, CVC ClinicDB and Kvasir dataset. The experimental results show that our proposed method can achieve a better trade-off in model size and performance. More importantly, the proposed QAMWN outperforms previous state-of-the-art methods in both metrics and visualization.Significance. We propose a lightweight SR network for endoscopy and achieves better performance with fewer parameters, which helps in clinical diagnosis of endoscopy.

miR-135a-5p Suppresses TBK1 and Activates NRF2/TXNIP Antioxidant Pathway in LPS-Driven ALI in Mice.

Objective: Acute inflammation and oxidative stress are present in large numbers in patients with acute lung injury (ALI). This investigation probed miR-135a-5p/TBK1 axis within ALI together with its new therapeutic target. Methods: MLE-12 cultures were treated with lipopolysaccharide (LPS) and transfected with miR-135a-5p mimics or TBK1 vector. An ALI mouse model was also established. Analysis was done on the relationships between TBK1 and miR-135a-5p. Inflammatory components, SOD, MDA, and ROS content were all assessed. Results: Obvious inflammatory lesions were observed in lung tissues of ALI mice. Overexpression of miR-135a-5p or TBK1 knockdown remarkably decreased IL-1ß, IL-6, and TNF-α serum concentrations and increased IL-10 level within lung tissues. Activated NRF2/TXNIP pathway and oxidative stress were additionally found within ALI murines, which were regulated by miR-315a-5p and TBK1. Further research revealed that miR-135a-5p negatively regulated TBK1 expression to mediate proinflammatory response and oxidative stress. Conclusion: miR-135a-5p targeted TBK1 to regulate inflammatory/oxidative stress responses in ALI. Such results might bring a new potential target for ALI treatment.

High TRB3 expression induces chondrocyte autophagy and senescence in osteoarthritis cartilage.

OBJECTIVE: Osteoarthritis is closely related to aging. Tribbles homologue 3 (TRB3) is found to display age-related expression and contributes to the regulation of cell proliferation, differentiation and fibrosis. In this study, we aimed to investigate the potential involvement of TRB3 in cartilage autophagy and aging in osteoarthritis. METHODS: Cartilage tissue samples were collected from osteoarthritis patients who received joint replacement and cadaveric donors. In osteoarthritis cartilage tissue, we analyzed autophagy- and senescence-associated proteins using immunohistochemistry and western blot (WB), in vitro, to confirm the role played by TRB3 in the process of autophagy, cell senescence, and inflammation, small interfering RNA (siRNA) was used for TRB3 knockdown in cells. RESULTS: We found increased level of p62, decreased level of microtubule-associated protein 1A/1B-light chain 3 (LC3) and beclin-1 in cartilage, and increased level of p16 and p21 in tissue samples collected from osteoarthritis patients, indicating decreased autophagy and increased cell senescence. TRB3 knockdown significantly rescued, in vitro, the reduced autophagy and elevated cell senescence in human chondrocyte. CONCLUSIONS: Interfering with TRB3 expression in cartilage may serve as a target in the prevention and treatment of age-related osteoarthritis.

Gains in biodiversity conservation and ecosystem services from the expansion of the planet's protected areas.

Protected areas safeguard biodiversity, ensure ecosystem functioning, and deliver ecosystem services to communities. However, only ~16% of the world's land area is under some form of protection, prompting international calls to protect at least 30% by 2030. We modeled the outcomes of achieving this 30 × 30 target for terrestrial biodiversity conservation, climate change mitigation, and nutrient regulation. We find that the additional ~2.8 million ha of habitat that would be protected would benefit 1134 ± 175 vertebrate species whose habitats currently lack any form of protection, as well as contribute to either avoided carbon emissions or carbon dioxide sequestration, equivalent to 10.9 ± 3.6 GtCO2 year-1 (28.4 ± 9.4% of the global nature-based climate-change mitigation potential). Furthermore, expansion of the protected area network would increase its ability to regulate water quality and mitigate nutrient pollution by 142.5 ± 31.0 MtN year-1 (28.5 ± 6.2% of the global nutrient regulation potential).

Future land-use competition constrains natural climate solutions.

Natural climate solutions (NCS) are an essential complement to climate mitigation and have been increasingly incorporated into international mitigation strategies. Yet, with the ongoing population growth, allocating natural areas for NCS may compete with other socioeconomic priorities, especially urban development and food security. Here, we projected the impacts of land-use competition incurred by cropland and urban expansion on the climate mitigation potential of NCS. We mapped the areas available for implementing 9 key NCS strategies and estimated their climate change mitigation potential. Then, we overlaid these areas with future cropland and urban expansion maps projected under three Shared Socioeconomic Pathway (SSP) scenarios (2020-2100) and calculated the resulting mitigation potential loss of each selected NCS strategy. Our results estimate a substantial reduction, 0.3-2.8 GtCO2 yr-1 or 4-39 %, in NCS mitigation potential, of which cropland expansion for fulfilling future food demand is the primary cause. This impact is particularly severe in the tropics where NCS hold the most abundant mitigation potential. Our findings highlight immediate actions prioritized to tropical areas are important to best realize NCS and are key to developing realistic and sustainable climate policies.

The downregulation of miR-22 and miR-372 may contribute to gestational diabetes mellitus through regulating glucose metabolism via the PI3K/AKT/GLUT4 pathway.

BACKGROUND: Identifying effective regulatory mechanisms will be significant for Gestational diabetes mellitus (GDM) diagnosis and treatment. METHODS: The expressions of miR-22 and miR-372 in placenta tissues from 75 pregnant women with GDM and 75 matched healthy controls and HRT8/SVneo cells (a model of insulin resistance) were analyzed by qPCR. The expressions of PI3K, AKT, IRS, and GLUT4 in high glucose-treated HRT8/SVneo cells transfected with miR-22 or miR-372 mimics or inhibitors was assessed by Western blot. A luciferase gene reporter assay was employed to verify miRNAs' target genes. RESULTS: The expressions of miR-22 and miR-372 in placental tissues from GDM patients and HRT8/SVneo cells were significantly decreased compared with the respective controls. The GLUT4 expression was significantly decreased in the placenta tissues of GDM and HRT8/SVneo cells with high glucose transfected with miR-22 and miR-372 inhibitors. We confirmed that SLC2A4, the gene encoding GLUT4, was a direct target of miR-22 and miR-372. In this study, we report that the lower expressions of miR-22 and miR-372 in placental tissue from GDM patients. CONCLUSION: Our results further suggested that the downregulations of miR-22 and miR-372 may contribute to GDM through regulating the PI3K/GLUT4 pathway.

Moving Object Detection and Tracking by Event Frame from Neuromorphic Vision Sensors.

Fast movement of objects and illumination changes may lead to a negative effect on camera images for object detection and tracking. Event cameras are neuromorphic vision sensors that capture the vitality of a scene, mitigating data redundancy and latency. This paper proposes a new solution to moving object detection and tracking using an event frame from bio-inspired event cameras. First, an object detection method is designed using a combined event frame and a standard frame in which the detection is performed according to probability and color, respectively. Then, a detection-based object tracking method is proposed using an event frame and an improved kernel correlation filter to reduce missed detection. Further, a distance measurement method is developed using event frame-based tracking and similar triangle theory to enhance the estimation of distance between the object and camera. Experiment results demonstrate the effectiveness of the proposed methods for moving object detection and tracking.

[Repair of Kuwadaâ ¡and â ¢ Achilles tendon defects through modified medial J-shaped approach with autologous fascia lata transplantation].

OBJECTIVE: To investigate the clinical effect of modified medial J-shaped incision of Achilles tendon combined with fascia lata transplantation in the treatment of Kuwada typeⅡand Ⅲ Achilles tendon defects. METHODS: From January 2016 to August 2018, the clinical data of 15 patients with KuwadaⅡand Ⅲ Achilles tendon defects treated with modified J-shaped approach with autologous fascia lata transplantation were retrospectively analyzed, including 14 males and 1 female, with an average age of 31.7 years old ranging from 24 to 43. There were 9 cases of KuwadaⅡdefect and 6 cases of KuwadaⅢ defect. Postoperative observations were made for incision complications, and the Arner-Lindholm scoring standard was used to evaluate the function of the affected foot at the last follow-up. RESULTS: All 15 cases were followed up from 3 to 16 months with an average of 9.2 months. No skin necrosis or infection occurred after operation, and no Achilles tendon rupture occurred again. According to the Arner-Lindholm scoring standard, 13 cases were excellent, 2 cases were good. CONCLUSION: Modified medial J-shaped incision is a satisfactory approach for repairing Achilles tendon defects. It is helpful to prevent postoperative incision complications, which double-strengthen the Achilles tendon strength, so that patients can perform early rehabilitation and functional exercises with satisfactory clinical results.

Threats to land and environmental defenders in nature's last strongholds.

Land and environmental defenders are a major bulwark against environmental destruction and biodiversity loss resulting from unsustainable nature resource extraction. Resultant conflicts can lead to violence against and deaths of these defenders. Along with mounting environmental pressures, homicides of these defenders are increasing globally. Yet, this issue has only recently started to receive scientific attention. While existing studies indicate the importance of socio-economic processes in driving such murders, spatially explicit global analyses considering environmental components are largely missing. Here, we take a broad spatial approach to assess relative contributions of environmental factors to the killing of environmental defenders. We find higher rates of such homicides are typically found in areas where limited or underutilized resources (e.g., freshwater, land and forests) are more available. Our results point towards a prevalent global land scarcity that results in industries targeting the last remaining strongholds for biodiversity and the environmental defenders within.

Calcium Carbonate@silica Composite with Superhydrophobic Properties.

In this paper, spherical calcium carbonate particles were prepared by using CaCl2 aqueous solution + NH3·H2O + polyoxyethylene octyl phenol ether-10 (OP-10) + n-butyl alcohol + cyclohexane inverse micro emulsion system. Then, nanoscale spherical silica was deposited on the surface of micron calcium carbonate by Stöber method to form the composite material. Scanning electron microscope (SEM), X-ray powder diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), Thermogravimetric analysis (TGA) and X-ray photoelectron spectroscopy (XPS) were used to characterize the morphology and structure of the composite material. It is found that the surface of the composite material has a micro-nano complex structure similar to the surface of a "lotus leaf", making the composite material show hydrophobicity. The contact angle of the cubic calcium carbonate, spherical calcium carbonate and CaCO3@SiO2 composite material were measured. They were 51.6°, 73.5°, and 76.8°, respectively. After modification with stearic acid, the contact angle of cubic and spherical CaCO3 were 127.1° and 136.1°, respectively, while the contact angle of CaCO3@SiO2 composite was 151.3°. These results showed that CaCO3@SiO2 composite had good superhydrophobicity, and the influence of material roughness on its hydrophobicity was investigated using the Cassie model theory.

The Measurement of the Inclination Angle of the Hamate and Analysis of the Inclination Angle for the Rotation Deformity of the Little Finger in the Fixation of the Carpometacarpal Joint.

OBJECTIVE: Complex base fractures of the fifth metacarpal bone and dislocation of the fifth carpometacarpal joint are more prone to internal rotation deformity of the little finger sequence after fixation with a transarticular plate. In the past, we have neglected that there is actually a certain angle of external rotation in the hamate surface of transarticular fixation. This study measured the inclination angle of the hamate surface relative to the fifth metacarpal surface for clinical reference. METHODS: In a prospective single-center study, we investigated the tilt angle of 60 normal hamates. The study included thin-layer computed tomography (CT) data from 60 patients from the orthopaedic clinic and inpatient unit from January 2017 to March 2020, including 34 men and 26 women who were 15~59 years old, average 35 years old. The CT data of 60 cases in Dicom format of the hand was input into Mimics and 3-Matics software for three-dimensional (3D) reconstruction and measuring the angle α between hamate surface and the fifth metacarpal surface. According to the possible placement of the transarticular plate on the fifth metacarpal surface, we measured the angle ß between the hamate surface 1 and the fifth metacarpal surface and the angle γ between the hamate surface 2 and the fifth metacarpal surface. RESULTS: The average angle between the hamate surface and the fifth metacarpal surface was 11.66°. The hamate surfaces 1 and 2 have an external rotation angle of 7.30° and 7.51° on average with respect to the fifth metacarpal surface, respectively. There is no statistically significant difference in the angles between the two groups (P > 0.05). CONCLUSIONS: The horizontal angle of the dorsal side of the hamate is different from the back of the fifth metacarpal surface, and the hamate has a certain external rotation angle with respect to the fifth metacarpal surface. No matter how the transarticular plate is placed, the plate always has a certain external rotation angle relative to the fifth metacarpal surface. When the fixation is across the fifth carpometacarpal joint, if the plate does not twist and shape, it will inevitably cause internal rotation of the fifth metacarpal, resulting in internal rotation deformity of the little finger sequence.

Invasive species trait-based risk assessment for non-native freshwater fishes in a tropical city basin in Southeast Asia.

Biological invasions have created detrimental impacts in freshwater ecosystems. As non-native freshwater species include economically beneficial, but also harmful, species, trait-based risk assessments can be used to identify and prevent the import of potentially invasive species. Freshwater fishes are one of the most evaluated freshwater taxa to date. However, such assessments have mostly been done in sub-temperate to temperate regions, with a general lack of such research in the tropics. In view of this knowledge gap, this study aims to determine if a different set of traits are associated with successful establishment of non-native fishes within the tropics. In tropical Southeast Asia, Singapore represents a suitable model site to perform an invasive species trait-based risk assessment for the tropical region given its susceptibility to the introduction and establishment of non-native freshwater fishes and lack of stringent fish import regulation. A quantitative trait-based risk assessment was performed using random forest to determine the relative importance of species attributes associated with the successful establishment of introduced freshwater fishes in Singapore. Species having a match in climate, prior invasion success, lower absolute fecundity, higher trophic level, and involvement in the aquarium trade were found to have higher establishment likelihood (as opposed to native distributional range and maximum size being among the commonly identified predictors in subtropical/temperate trait-based risk assessments). To minimize invasive risk, incoming freshwater fishes could be screened in future for such traits, allowing lists of prohibited or regulated species to be updated. The findings could also potentially benefit the development of invasive species action plans and inform management decisions in the Southeast Asian region. Considering a geographical bias in terms of having relatively less documentation of biological invasions in the tropics, particularly Asia, this study highlights the need to perform more of such risk assessments in other parts of the tropics.

Global potential and limits of mangrove blue carbon for climate change mitigation.

Despite the outsized role of mangrove forests in sustaining biodiversity, ecosystem function, and local livelihoods, the protection of these vital habitats through blue carbon financing has been limited.1,2 Here, we quantify the extent of this missed conservation and financial opportunity, showing that the protection of ∼20% of the world's mangrove forests (2.6 Mha) can be funded through carbon financing. Of these investible areas, 1.1-1.3 Mha can be financially sustainable over a 30-year time frame based on carbon prices of US$5-9.4 t-1CO2e. This contributes up to 29.8 MtCO2e year-1 and yields a return on investment of ∼US$3.7 billion per year. Our results point toward a disproportionately large potential of blue carbon finance that can be leveraged to meet national-level climate mitigation goals, particularly if combined with other conservation interventions that further safeguard carbon stocks and biodiversity in these irreplaceable forests. Robust information on return on investment highlights the potential for currently underutilized tropical coastal carbon credit projects.

Carbon prospecting in tropical forests for climate change mitigation.

Carbon finance projects that protect tropical forests could support both nature conservation and climate change mitigation goals. Global demand for nature-based carbon credits is outpacing their supply, due partly to gaps in knowledge needed to inform and prioritize investment decisions. Here, we show that at current carbon market prices the protection of tropical forests can generate investible carbon amounting to 1.8 (±1.1) GtCO2e yr-1 globally. We further show that financially viable carbon projects could generate return-on-investment amounting to $46.0b y-1 in net present value (Asia-Pacific: $24.6b y-1; Americas: $19.1b y-1; Africa: $2.4b y-1). However, we also find that ~80% (1.24 billion ha) of forest carbon sites would be financially unviable for failing to break even over the project lifetime. From a conservation perspective, unless carbon prices increase in the future, it is imperative to implement other conservation interventions, in addition to carbon finance, to safeguard carbon stocks and biodiversity in vulnerable forests.