Asunto(s)
Anticuerpos Antivirales/sangre , Virus de la Parainfluenza 5/genética , Virus de la Parainfluenza 5/aislamiento & purificación , Infecciones del Sistema Respiratorio/veterinaria , Infecciones por Rubulavirus/veterinaria , Animales , Heces/virología , Caballos , Humanos , Metagenómica , Nariz/virología , Filogenia , Infecciones del Sistema Respiratorio/virología , Infecciones por Rubulavirus/sangre , Infecciones por Rubulavirus/virología , Homología de SecuenciaRESUMEN
BACKGROUND: In May 2018, a 8 year old thoroughbred mare died at an equestrian club in Changji, Xinjiang, China. The horse had been imported from the United States in 2013. She became pregnant in December 2016 but, after foaling, gradually lost weight and died in May 2018. This study aim to identify the pathogen, who cause of horse death, using virome. RESULTS: We have identified an Equ1-like virus from the fecal virome of a dead thoroughbred mare in China. Full genomic sequencing and phylogenetic analysis of the virus, tentatively named "kirkovirus Cj-7-7", showed that it was closely related to kirkovirus Equ1 and clustered together with po-circo-like viruses 21, 22, 41, and 51, suggesting that it should be assigned to the proposed family "Kirkoviridae". An epidemiological investigation showed that kirkovirus Cj-7-7 circulates in horses of northern Xinjiang and may specifically infect intestinal cells. CONCLUSIONS: Our findings demonstrate the genetic diversity and geographic distribution of Kirkoviruses, and the prevalence of Kirkovirus Cj-7-7 in Xinjiang, China.
Asunto(s)
Infecciones por Virus ADN/veterinaria , Virus ADN/clasificación , Virus ADN/aislamiento & purificación , Heces/virología , Enfermedades de los Caballos/virología , Animales , China , Análisis por Conglomerados , Infecciones por Virus ADN/patología , Infecciones por Virus ADN/virología , Virus ADN/genética , Genoma Viral , Enfermedades de los Caballos/patología , Caballos , Filogenia , Análisis de Secuencia de ADN , Homología de Secuencia , Estados Unidos , Secuenciación Completa del GenomaRESUMEN
In 2015, a novel equine parvovirus, equine parvovirus-cerebrospinal fluid (EqPV-CSF), was identified from cerebrospinal fluid of a horse with neurological signs and lymphocytosis in USA. In our study, an EqPV-CSF-like virus was detected from 15 serum samples of 65 imported thoroughbred horses during custom quarantine in north Xinjiang province, China. Further field investigation in several major horse-producing areas in Xinjiang using specific PCR showed that this virus was detected mainly in thoroughbred horses (39/154 positive) previously imported, not in local breeds (0/127 positive). Phylogenetic analysis based on NS1 gene showed the virus was grouped together with EqPV-CSF in genus Copiparvovirus. The study presents valuable information showing that EqPV-CSF-like virus was likely exotic and introduced into Xinjiang through international trade of horses.
