[Sources, Distribution of Main Controlling Factors, and Potential Ecological Risk Assessment for Heavy Metals in the Surface Sediment of Hainan Island North Bay, South China].

The distribution characteristics of the content of As, Cr, Cu, Hg, Pb, Zn, and Cd were researched based on the geochemical analysis of 159 surface sediment samples in Haikou Bay, Puqian Bay, Dongzhai Harbor, and Mulan Bay Mathematical statistics methods were used to interpret the sources of heavy metals, and the main controlling factors for heavy metal distribution were confirmed, having been analyzed qualitatively and semi-quantitatively. The results showed that the average contents of As, Cd, Cr, Cu, Hg, Pb, and Zn were 8.40, 0.06, 32.50, 8.32, 0.02, 18.77, and 35.87 µg·g-1, respectively. High contents of Cr, Cu, Hg, Pb, and Zn are mainly distributed in estuaries and harbors. The content of As increased gradually from south to north in the research area, while Cd gathered mainly in Haikou Bay. The source of Cu, Zn, Hg, Pb, Cr, and Cd in sediment was terrestrial input, which was controlled by anthropogenic pollution and migration of weathered products from mother rock. The source of As could be overseas material input. The grain size of sediment was the main factor controlling the contents of Cu, Zn, Hg, Pb, and Cr, and it was found that sediments with a finer grain size have more heavy metals adsorbed. The high As content was controlled mainly by the natural geological background factor, while the content of Cd reflected the difference in regional pollution caused by differing development in cities. Three surface sediment samples were evaluated as class Ⅱ of the National Marine Sediment Standard due to the content of As, while six surface sediment samples were evaluated as class Ⅱ due to the content of Cr, of which the pollution degree was moderate. The concentrations of Cd, Cu, Hg, Pb, and Zn in all of the 159 sediment samples fall in the Ⅰ class, of which the pollution degree is low. The degree of pollution for seven heavy metals were arranged in the following order:As > Cr > Pb > Zn > Cu > Cd > Hg. The potential ecological risk assessment results indicated that all heavy metals in surface sediment caused low levels of pollution generally, and that each heavy metal element was at a low ecological risk level. The order for the ecological risk of the seven heavy metals was:As > Hg > Cd > Pb > Cu > Cr > Zn; therefore, As was the main ecological risk factor. In general, the potential ecological risk for heavy metals was low, which illustrated that the marine environment in the study area was excellent.

Effects of alfalfa saponin extract on mRNA expression of Ldlr, LXRα, and FXR in BRL cells.

We studied the effects of alfalfa saponin extract (ASE) on low density lipoprotein receptor (Ldlr), liver X receptor α (LXRα), and farnesoid X receptor (FXR) in normal and hyperlipidemic Buffalo rat liver (BRL) cells. Normal and hyperlipidemic BRL cells were divided into eight groups: normal, or normal cells treated with 50, 100, and 150 mg/L ASE, hyperlipidemic, or hyperlipidemic cells treated with 50, 100, and 150 mg/L ASE. After treatment for 24 h, Ldlr, LXRα, and FXR mRNA expression levels were measured by quantitative real-time polymerase chain reaction (qRT-PCR). Data showed that mRNA expression of Ldlr in normal BRL cells was significantly up-regulated by ASE treatment and mRNA expressions of LXRα and FXR were significantly down-regulated both in normal and hyperlipidemic BRL cells after ASE treatment. Thus, ASE might ameliorate hepatic steatosis by regulating genes involved in cholesterol metabolism, including up-regulation of Ldlr as well as down-regulation of LXRα and FXR.