Rapid response in relapsed follicular lymphoma with massive chylous ascites to anti-CD19 CAR T therapy using Piggy Bac: A case report.

CD19-directed chimeric antigen receptor (CAR) T cell therapy has been shown to achieve a considerably durable response in patients with refractory or relapsed B cell non-Hodgkin lymphomas, as seen from the results of Zuma-1, Zuma-5, and other clinical trials. Most of these CARs were generated by lentivirus or reverse adenovirus. It is rare to see CARs using non-viral vectors, such as Piggy Bac (pb), in treating lymphoma patients with active diseases. Generally, patients with a high tumor burden tend to have a higher rate of severe cytokine release syndrome (CRS) or neurological events as reported in the literature. Patients with symptomatic pleural effusions are excluded from the Zuma-1 trial because of the risk of severe CRS. We report here that a patient with relapsed follicular lymphoma with bulky disease and massive chylous ascites failed several lines of chemotherapy. After infusion of the CD19-directed pbCAR-T cells at 6 × 106 cells/kg, the patient had a rapid response and achieved a nearly complete metabolic remission on day 28. There was only grade 1 CRS, and no neurotoxicity occurred. The CAR-T cells reached a peak level on day 14 and spread into the ascites and expanded for 3 months. This might be the first case reported for pbCAR-T cells to treat relapsed follicular lymphoma directly. The long-term efficacy will be observed, and more patients be tested in the future. Clinical Trial Registration: https://ClinicalTrials.gov, identifier NCT05472610.

An alternative for the anode materials of nickel metal hydride batteries: an AB3-type La0.6Gd0.2Mg0.2Ni2.6Co0.3Al0.1 hydrogen storage alloy.

In order to satisfy the demand for the cyclic stability of commercial Ni-MH anodes, a PuNi3-type La0.6Gd0.2Mg0.2Ni2.6Co0.3Al0.1 alloy with excellent overall electrochemical properties was prepared by annealing the as-cast alloy sample at different temperatures for a week. The alloy had the highest PuNi3-type content of 86.9 wt% (1073 K), which offered a capacity retention of 69.6% after 100 cycles. However, 23.7 wt% PuNi3 type phase of the alloy constantly converted into the Ce2Ni7 type phase within a temperature increase of 50 °C, which improved the capacity retention by 12.1% under the same discharge capacity. We found that the addition of Gd did not change the stacked [LaMgNi4]/[LaNi5] superlattice and it maintained the structural stability of the crystal as well as its anti-corrosion, which is also a key factor to improve cyclic stability. These findings imply that alloys with both PuNi3-type and Ce2Ni7-type multiphase structures can be considered as a new choice for hydrogen storage.