Novel Finding on How Melatonin and Nanoselenium Alleviate 2,4-D Butylate Stress in Wheat Plants.

Nanoselenium (nano-Se) or melatonin (MT) foliar spray reduces pesticide stress by stimulating plant secondary metabolism and antioxidant capacity. However, the effects of nano-Se and MT biofortification on the interaction between plant secondary metabolic pathways and rhizosphere microbes in mitigating 2,4-D butyrate stress remain unknown. Compared to nano-Se or MT treatment alone, nano-Se and MT combined application increased the antioxidant enzyme activities and decreased the MDA (25.0%) and H2O2 (39.3%) contents with 2,4-D butylate exposure. Importantly, they enhance the soil enzymes (S-FDA by 53.1%), allelochemicals (luteolin by 164.1% and tricin by 147.3%), as well as plant secondary metabolites (JA by 63.3% and 193.3% in leaves and roots) levels. It also improved the beneficial microbial abundance of Comamonadaceae, Sphingomonadaceae, and Rhodobacteraceae in the rhizosphere soil. In conclusion, nano-Se and MT alleviate 2,4-D butylate stress in wheat plants by enabling the interaction between rhizosphere microorganisms, allelopathic substances, and secondary metabolites.

Metabolomic Analysis on the Mechanism of Nanoselenium Biofortification Improving the Siraitia grosvenorii Nutritional and Health Value.

Nanoselenium (nano-Se) foliar application is crucial for enhancing plant health. However, the mechanism by which nano-Se biofortification promotes the nutritional components of Siraitia grosvenorii remains unclear. In this study, nano-Se foliar application increased the carbohydrate and amino acid contents, including glucose (23.6%), fructose (39.7%), sucrose (60.6%), tryptophan (104.5%), glycine (85.9%), tyrosine (78.4%), phenylalanine (60.1%), glutamic acid (63.4%), and proline (52.5%). Nano-Se application enhanced apigenin (3.8 times), syringic acid (0.7 times), and 4-hydroxy-3,5-dimethoxycinnamic acid (1.4 times) of the phenylpropane biosynthesis pathways. Importantly, the SgCDS (31.1%), CYP-P450 (39.1%), and UGT (24.6%) were induced by nano-Se, which enhanced the mogroside V content (16.2%). Compared to the control, nano-Se treatment dramatically enhanced aromatic substances, including 2-butanone (51.9%), methylpropanal (146.3%), n-nonanal dimer (141.7%), pentanal (52.5%), and 2-pentanone (46.0%). In summary, nano-Se improves S. grosvenorii quality by increasing nutrients and volatile organic compounds and adjusting the phenylpropane pathway.

Bensulfuron-Methyl, Terbutylazine, and 2,4-D Butylate Disturb Plant Growth and Resistance by Deteriorating Rhizosphere Environment and Plant Secondary Metabolism in Wheat Seedlings.

Frequent and improper use of herbicides disrupts a plant's metabolism, causing oxidative stress that degrades crop quality. However, few studies have examined the inhibitory effects of herbicides on plant growth and defense mechanisms in terms of their impact on soil quality and crop rhizosphere. Therefore, the current study investigated the detrimental impacts of six typical and multilevel herbicides on the microbial community and signal molecules in the soil as well as on the levels of hormones and secondary metabolites in wheat seedlings. Interestingly, bensulfuron-methyl, terbutylazine (TBA), and 2,4-D butylate significantly induced oxidative damage while reducing the number of phytohormones (salicylic acid and jasmonic acid) and secondary metabolites (tricin, quercetin, and caffeic acid) in the roots and leaves compared with the controls, isoproturon, fenoxaprop-p-ethyl, and pretilachlor. At twice the recommended levels (2×), they also decreased the microbial α diversity and, in particular, the abundance of Gammaproteobacteria, Alphaproteobacteria, Actinobacteria, Bacteroidia, Verrucomicrobia, Bacilli, Acidimicrobiia, Deltaproteobacteria, and Gemmatimonadetes by disrupting the level of enzymes (e.g., urease and sucrase) and metabolites (indole-3-acetic acid, salicylic acid, apigenin, 4-hydroxybenzoic acid, DIMBOA, and melatonin) in the rhizosphere soil. Overall, significant exposure to herbicides may inhibit wheat growth by disturbing the microbial composition in the rhizosphere soil and the distribution of secondary metabolites in wheat seedlings.

Nano-selenium enhances the antioxidant capacity, organic acids and cucurbitacin B in melon (Cucumis melo L.) plants.

Pesticides are widely used in melon production causing safety issues around the consumption of melon and increasing pathogen and insect tolerance to pesticides. This study investigated whether a nano-selenium (Nano-Se) spray treatment can improve resistance to biological stress in melon plants, reducing the need for pesticides, and how this mechanism is activated. To achieve this, we examine the ultrastructure and physio-biochemical responses of two melon cultivars after foliar spraying with Nano-Se. Nano-Se treatment reduced plastoglobulins in leaf mesophyll cells, thylakoid films were left intact, and compound starch granules increased. Nano-Se treatment also increased root mitochondria and left nucleoli intact. Nano-Se treatment enhanced ascorbate peroxidase, peroxidase, phenylalanine ammonia lyase, ß-1,3-glucanase, chitinase activities and their mRNA levels in treated melon plants compared to control plants (without Nano-Se treatments). Exogenous application of Nano-Se improved fructose, glucose, galactitol, stachyose, lactic acid, tartaric acid, fumaric acid, malic acid and succinic acid in treated plants compared to control plants. In addition, Nano-Se treatment enhanced cucurbitacin B and up-regulated eight cucurbitacin B synthesis-related genes. We conclude that Nano-Se treatment of melon plants triggered antioxidant capacity, photosynthesis, organic acids, and up-regulated cucurbitacin B synthesis-related genes, which plays a comprehensive role in stress resistance in melon plants.

Dissipative behavior, residual pattern, and risk assessment of four pesticides and their metabolites during tea cultivation, processing and infusion.

BACKGROUND: In recent years, metabolic products of pesticides have gained much attention due to their substantial characteristics as organic pollutants. So far, the behavior and metabolite levels of pesticide metabolites in crops have not been characterized well. In the present study, four registered pesticides (imidacloprid, diafenthiuron, malathion and chlorothalonil) were applied on tea plants in Fujian and Sichuan to characterize their metabolites residue pattern and dietary risk. RESULTS: Four pesticides dissipated first-order kinetics in the fresh tea leaves with the half-lives of 1.4-3.8 days. Nine metabolites were detected in the fresh tea leaves and green tea after processing. The metabolites residues showed an increasing trend first and then declined after treatment, and reached the maximum near the half-lives of pesticide. Compared with the parent pesticide, the total residue and acute risk (included the metabolites) increased by 1.7-105.2 times. Some metabolites, especially those whose parent pesticides have high water solubility and low Log Kow, will be more easily transferred to tea infusion. CONCLUSION: Pesticides were metabolized rapidly on tea plants after application, but the production of metabolites increased the health risk of tea consumption. These results could provide insights to use the pesticides in tea gardens and risk monitoring after application. © 2022 Society of Chemical Industry.

Nanoselenium integrates soil-pepper plant homeostasis by recruiting rhizosphere-beneficial microbiomes and allocating signaling molecule levels under Cd stress.

Most studies have focused on regulation in a metabolic pathway in response to exogenous selenium under cadmium stress, rather than the change of key factors in soil and pepper plants. In this study, the correlations in environmental variables, microorganisms, metabolic pathways, Se and Cd morphology under nano-Se intervention were examined using metabolomics and microbial diversity in rhizosphere soil and pepper plants. The principal forms of Se in the soils were Se (VI) and SeCys, while SeMet and MeSeCys were the main components in the root, stem, leaves, and fruits in the treatment of nano-Se (5 and 20 mg/L) relative to the control. Soil enzymes,metabolites (fluorescein diacetate, urease, brassinolide, and p-hydroxybenzonic acid), and plant metabolites (rutin, luteolin, brassinolide, and abscisic acid) were remarkably enhanced by nano-Se fortification. The bio-enhancement of nano-Se can boost the beneficial microorganisms of Gammaproteobacteria, Alphaproteobacteria, Bacteroidia, Gemmatimonadetes, Deltaproteobacteria, and Anaerolineae in rhizosphere soil. Changes in microbial community were found to be strongly linked to the environment index, enzymes, soil metabolites, Se forms, which reduced Cd bioavailability and Cd accumulation in pepper plants. In conclusion, the nano-Se application integrates soil-plant balance by improving soil qualities and assigning signaling molecule levels in rhizosphere soil and pepper plants.

Multi-residue analytical method development and risk assessment of 56 pesticides and their metabolites in tea by chromatography tandem mass spectroscopy.

A modified QuEChERS method coupled to chromatography tandem mass spectroscopy was established and used to identify 56 pesticides and 21 metabolites residues in tea samples. The average recoveries for the target compounds ranged from 71% to 109% with RSDs of 1-17%. Pesticides and metabolites residues in 248 tea samples from China were investigated by the developed method, and the dietary intake risk for consumers was estimated. The results showed that 36 pesticides and 14 metabolites were detected with concentrations of 0.0050-7.7 mg/kg. There are unlikely to present a public health concern for Chinese consumers. The acute risk of pesticides would be exaggerated when calculated by the residue level in tea instead of tea infusion. The unauthorized and banned pesticide contributed to the chronic and acute hazard index by 68% and 36%, respectively. The cumulative risk of residual pesticides and their metabolites on human health deserve further attention.

Nanoselenium Enhanced Wheat Resistance to Aphids by Regulating Biosynthesis of DIMBOA and Volatile Components.

The mechanism of nanoselenium (nano-Se) improving the resistance induced by plant components to aphids is unclear. In this study, foliar sprayed nano-Se (5.0 mg/L) could significantly reduce the Sitobion avenae number (36%) compared with that in the control. Foliar application of nano-Se enhanced the antioxidant capacity by reducing malondialdehyde (MDA) and increasing GSH-Px, CAT, GSH, Pro, and VE concentrations in wheat seedlings. The phenylpropane pathway was activated by nano-Se biofortification, which increased apigenin and caffeic acid concentrations. The high-level expression of the related genes (TaBx1A, TaBx3A, TaBx4A, TaASMT2, and TaCOMT) induced the promotion of melatonin (88.6%) and 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one (DIMBOA) (64.3%). Different ratios of the secondary metabolites to nano-Se were taken to examine the effects on resistance of wheat to S. avenae. The results revealed that the combination of nano-Se and melatonin could achieve the best overall performance by reducing the S. avenae number by 52.2%. The study suggests that the coordinated applications of nano-Se and melatonin could more effectively improve the wheat resistance to aphids via the promotion of volatile organic compound synthesis and modulation in phenylpropane and indole metabolism pathways.

Nanoselenium transformation and inhibition of cadmium accumulation by regulating the lignin biosynthetic pathway and plant hormone signal transduction in pepper plants.

Selenium (Se) can promote the growth and resistance of agricultural crops as fertilizers, while the role of nano-selenium (nano-Se) against Cd remains unclear in pepper plants (Capsicum annuum L.). Biofortification with nano-Se observably restored Cd stress by decreasing the level of Cd in plant tissues and boosting the accumulation in biomass. The Se compounds transformed by nano-Se were primarily in the form of SeMet and MeSeCys in pepper tissues. Differential metabolites and the genes of plant signal transduction and lignin biosynthesis were measured by employing transcriptomics and determining target metabolites. The number of lignin-related genes (PAL, CAD, 4CL, and COMT) and contents of metabolites (sinapyl alcohol, phenylalanine, p-coumaryl alcohol, caffeyl alcohol, and coniferaldehyde) were remarkably enhanced by treatment with Cd1Se0.2, thus, maintaining the integrity of cell walls in the roots. It also enhanced signal transduction by plant hormones and responsive resistance by inducing the biosynthesis of genes (BZR1, LOX3, and NCDE1) and metabolites (brassinolide, abscisic acid, and jasmonic acid) in the roots and leaves. In general, this study can enable a better understanding of the protective mechanism of nano-Se in improving the capacity of plants to resist environmental stress.

Nanoselenium foliar application enhances biosynthesis of tea leaves in metabolic cycles and associated responsive pathways.

An emerging stress of pesticides in plant and soil is closely watched as it affects crop antioxidant systems, nutritional quality, and flavor. Although selenium (Se) can enhance the resistance of plants, the protective mechanism of nanoselenium is still not known under the long-term pesticide stress in tea trees. In this study, we investigated the potential effects of foliar application of nanoselenium for a two-year field experiment on tea plants under pesticide-induced oxidative stress. Compared to control, nano-Se (10 mg/L) markedly enhanced the protein, soluble sugar, carotenoid, tea polyphenols, and catechins contents. High levels of theanine, glutamic acid, proline, and arginine were found to be induced most likely by adjusting the GS-GOGAT cycle. Se-supplementation may promote tea leaves' secondary metabolism, thus increasing the accumulation of total phenols and flavonoids (apigenin, kaempferol, quercetin, myricetin, and rutin). It also minimized the accumulation of malondialdehyde, hydrogen peroxide, and superoxide anion by activating the antioxidants enzymes including in the AsA-GSH cycle. Selenium-rich tea also showed better fragrance and flavor. In summary, nano-Se can ameliorate the nutrients quality and abiotic stresses resistance of crops.

Nanoselenium Foliar Applications Enhance the Nutrient Quality of Pepper by Activating the Capsaicinoid Synthetic Pathway.

Increasing the crop quality through enhancement of plant health is a challenging task. In this study, nanoselenium (nano-Se) was sprayed on pepper leaves, and the pepper components were compared to those of selenite. It was found that nano-Se (20 mg/L) resulted in a greater performance of plant health. It increased the chlorophyll and soluble sugar levels, which could activate phenylpropane and branched-chain fatty acid pathways, as well as AT3-related enzymes and gene expressions. These led to an enhancement for the synthesis of capsaicinoids, flavonoids, and total phenols. The nano-Se treatment also significantly promoted the expression of phyto-hormones synthesis genes, and consequently increased jasmonic, abscisic, and salicylic acid levels. Proline pathway-related compounds were increased, which could decrease the malondialdehyde and hydroxyl radical levels in crops. This study shows that nano-Se activated capsaicinoid pathways by enhancing photosynthesis and raising soluble sugar levels. The capsaicinoid contents in peppers were then increased, which consequently promoted the accumulation of secondary metabolites and antioxidants.