VO2 /MoO3 Heterojunctions Artificial Optoelectronic Synapse Devices for Near-Infrared Optical Communication.

Artificial optoelectronic synapses (OES) have attracted extensive attention in brain-inspired information processing and neuromorphic computing. However, OES at near-infrared wavelengths have rarely been reported, seriously limiting the application in modern optical communication. Herein, high-performance near-infrared OES devices based on VO2 /MoO3 heterojunctions are presented. The textured MoO3 films are deposited on the sputtered VO2 film by using the glancing-angle deposition technique to form a heterojunction device. Through tuning the oxygen defects in the VO2 film, the fabricated VO2 /MoO3 heterojunction exhibits versatile electrical synaptic functions. Benefiting from the highly efficient light harvesting and the unique interface effect, the photonic synaptic characteristics, mainly including the short/long-term plasticity and learning experience behavior are successfully realized at the O (1342 nm) and C (1550 nm) optical communication wavebands. Moreover, a single OES device can output messages accurately by converting light signals of the Morse code to distinct synaptic currents. More importantly, a 3 × 3 artificial OES array is constructed to demonstrate the impressive image perceiving and learning capabilities. This work not only indicates the feasibility of defect and interface engineering in modulating the synaptic plasticity of OES devices, but also provides effective strategies to develop advanced artificial neuromorphic visual systems for next-generation optical communication systems.

A high-sensitivity SnSe/Si heterojunction position-sensitive detector for ultra-low power detection.

Position-sensitive detectors (PSDs) based on the lateral photovoltaic effect are crucial components in non-contact distance measurement, process control, guidance systems, and other related applications. However, PSDs are challenging due to the narrow spectral range and low sensitivity, limiting further practical application. Here, we present an ultra-sensitive SnSe/Si PSD device. A large-area uniform SnSe nanorod (NR) array film was grown on Si using a glancing-angle magnetron sputtering deposition technique and a SnSe/Si heterojunction PSD device was fabricated. PSDs exhibit an excellent photoresponse in a wide spectral range of 405-980 nm, showing an ultrahigh position sensitivity of 1517.4 mV mm-1 and an excellent spectral sensitivity of 4 × 104 V W-1. More importantly, the detection limit power of the device is as low as 10 nW, indicating the outstanding potential for weak light detection. Based on the unique structural features and interface effect, the mechanisms for the remarkable performance of the fabricated SnSe/Si PSD device are clarified. This work indicates the large potential of SnSe/Si heterojunctions as a promising material for ultrasensitive optical position-sensitive devices.

High-Performance Flexible Broadband Photothermoelectric Photodetectors Based on Tellurium Films.

Mid- and far-infrared photodetectors that can operate at room temperature are essential for both civil and military applications. However, the widespread use of mid-to-far-infrared photonic technology faces challenges due to the need for low-temperature cooling of existing commercial semiconductors and the limited optical absorption efficiency of two-dimensional materials. We have utilized the photothermoelectric effect to fabricate a self-powered, broadband, and high-performance photodetector based on a one-dimensional tellurium nanorod array film. The device surpasses energy band gap limitations, functioning even at wavelengths up to approximately 10,600 nm. In particular, the detectivity of the device can reach 4.8 × 109 Jones at 4060 nm under room-temperature conditions, which is an order of magnitude higher than that of commercially available photodetectors. It demonstrates fast response and recovery times of 8.3 and 8.8 ms. Furthermore, the device demonstrates outstanding flexibility withstanding over 300 bending cycles and environmental stability. These results suggest a viable approach for designing and developing high-performance, room-temperature, wearable optoelectronic devices.

More Reliable Neighborhood Contrastive Learning for Novel Class Discovery in Sensor-Based Human Activity Recognition.

Human Activity Recognition (HAR) systems have made significant progress in recognizing and classifying human activities using sensor data from a variety of sensors. Nevertheless, they have struggled to automatically discover novel activity classes within massive amounts of unlabeled sensor data without external supervision. This restricts their ability to classify new activities of unlabeled sensor data in real-world deployments where fully supervised settings are not applicable. To address this limitation, this paper presents the Novel Class Discovery (NCD) problem, which aims to classify new class activities of unlabeled sensor data by fully utilizing existing activities of labeled data. To address this problem, we propose a new end-to-end framework called More Reliable Neighborhood Contrastive Learning (MRNCL), which is a variant of the Neighborhood Contrastive Learning (NCL) framework commonly used in visual domain. Compared to NCL, our proposed MRNCL framework is more lightweight and introduces an effective similarity measure that can find more reliable k-nearest neighbors of an unlabeled query sample in the embedding space. These neighbors contribute to contrastive learning to facilitate the model. Extensive experiments on three public sensor datasets demonstrate that the proposed model outperforms existing methods in the NCD task in sensor-based HAR, as indicated by the fact that our model performs better in clustering performance of new activity class instances.

Leveraging diverse cell-death patterns to predict the prognosis, immunotherapy and drug sensitivity of clear cell renal cell carcinoma.

Clear cell renal cell carcinoma (ccRCC) poses clinical challenges due to its varied prognosis, tumor microenvironment attributes, and responses to immunotherapy. We established a novel Programmed Cell Death-related Signature (PRS) for ccRCC assessment, derived through the Least Absolute Shrinkage and Selection Operator (LASSO) regression method. We validated PRS using the E-MTAB-1980 dataset and created PCD-related clusters via non-negative matrix factorization (NMF). Our investigation included an in-depth analysis of immune infiltration scores using various algorithms. Additionally, we integrated data from the Cancer Immunome Atlas (TCIA) for ccRCC immunotherapy insights and leveraged the Genomics of Drug Sensitivity in Cancer (GDSC) database to assess drug sensitivity models. We complemented our findings with single-cell sequencing data and employed the Clinical Proteomic Tumor Analysis Consortium (CPTAC) and qRT-PCR to compare gene expression profiles between cancerous and paracancerous tissues. PRS serves as a valuable tool for prognostication, immune characterization, tumor mutation burden estimation, immunotherapy response prediction, and drug sensitivity assessment in ccRCC. We identify five genes with significant roles in cancer promotion and three genes with cancer-suppressive properties, further validated by qRT-PCR and CPTAC analyses, showcasing gene expression differences in ccRCC tissues. Our study introduces an innovative PCD model that amalgamates diverse cell death patterns to provide accurate predictions for clinical outcomes, mutational profiles, and immune characteristics in ccRCC. Our findings hold promise for advancing personalized treatment strategies in ccRCC patients.

All-in-One Optoelectronic Logic Gates Enabled by Bipolar Spectral Photoresponse of CdTe/SnSe Heterojunction.

Optoelectronic logic gate devices (OLGDs) have attracted significant attention in high-density information processors; however, multifunctional logic operation in a single device is technically challenging due to the unidirectional electrical transport. In this work, we deliberately design all-in-one OLGDs based on self-powered CdTe/SnSe heterojunction photodetectors. The SnSe nanorod (NR) array is grown on the sputtered CdTe film via a glancing-angle deposition technique to form a heterojunction device. At the interface, the photovoltaic (PV) effect in the CdTe/SnSe heterojunction and the photothermoelectric (PTE) effect from the SnSe NRs are combined together to induce the reversed photocurrent, leading to a unique bipolar spectral response. The competition between PV and PTE in different spectral ranges is thus employed to control the photocurrent polarity, and five basic logic gates of OR, AND, NAND, NOR, and NOT can be performed just with a single heterojunction. Our findings indicate the large potentials of the CdTe/SnSe heterojunctions as logic units in next-generation sensing-computing systems.

Squeeze-Type Piezoelectric Inkjet Printhead Actuating Waveform Design Method Based on Numerical Simulation and Experiment.

The piezoelectric inkjet printing technique has been commonly used to produce conductive graphics. In this paper, a trapezoidal waveform design method for squeeze-type piezoelectric inkjet printhead is presented to provide a modified steady ejection and optimal droplet shape, in which a coupled multi-physics model of a piezoelectric inkjet printhead is developed. This research describes the effects of parameters, including rising time tr, falling time tf, and dwelling time td, of the trapezoidal waveform on the pressure at the nozzle through numerical simulations. These parameters are initially optimized based on numerical simulations and further optimized based on experimental results. When the printhead is actuated by the optimized waveform with the tr = 5 µs, td = 10 µs, and tf = 2 µs, the droplets are in optimal shape, and their size is about half the diameter of the nozzle. The experimental results validate the efficacy of this waveform design method, which combines numerical simulation and experiment, as well as demonstrating that ink droplet formation can be studied from the point of pressure variation at the nozzle.

Exogenous Melatonin Modulates Physiological Response to Nitrogen and Improves Yield in Nitrogen-Deficient Soybean (Glycine max L. Merr.).

Melatonin (MT) is a key plant growth regulator. To investigate its effect at different growth stages on the yield of soybean under nitrogen deficiency, 100 µM MT was applied to soybean supplemented with zero nitrogen (0N), low nitrogen (LN), and control nitrogen (CK) levels, during the plant vegetative growth (V3) and filling (R5) stages. This study revealed that the application of MT mainly enhanced the nitrogen fixation of plants by increasing the root nodule number and provided more substrates for glutamine synthetase (GS) under 0N supply. However, under the LN supply, more ammonium was assimilated through the direct promotion of nitrate reductase (NR) activity by MT. MT enhanced the activity of ammonium-assimilation-related enzymes, such as GOGAT and GDH, and the expression of their coding genes, promoted the synthesis of chlorophyll and amino acids, and increased the photosynthetic capacity under nitrogen deficiency. Exogenous MT directly upregulated the expression of genes involved in the photosynthetic system and stimulated dry-matter accumulation. Thus, MT alleviated the inhibitory effect of nitrogen deficiency on soybean yield. This mitigation effect was better when MT was applied at the V3 stage, and the seed weight per plant increased by 16.69 and 12.20% at 0N and LN levels, respectively. The results of this study provide a new theoretical basis to apply MT in agriculture to improve the resilience of soybean plants to low nitrogen availability.

Immunostimulatory activity of soybean hull polysaccharide on macrophages.

Water-soluble polysaccharide isolated from soybean hull and fractionated using ion-exchange chromatography were investigated to determine their molecular characteristics and immunostimulating activity. In the present study, soybean hull polysaccharide (SHP) was separated and purified to obtain three main fractions (F1, F2 and F3), and their chemical and monosaccharide compositions were analyzed. SHP was mainly composed of carbohydrates (64.3%), proteins (16.2%) and sulfates (12.5%), with minor levels of uronic acid (3.2%), and predominantly contained glucose and mannose as monosaccharides. Moreover, when compared with cells treated with RPMI medium, SHP was revealed to promote the proliferation and pinocytosis of RAW264.7 cells, and to enhance the production of nitric oxide (NO), tumor necrosis factor (TNF)-α, interleukin (IL)-1ß and IL-6. Furthermore, flow cytometry demonstrated that CD11b and CD40 were involved in the immune regulation of RAW264.7 cells by SHP. Moreover, western blotting and other experiments revealed that SHP, a type of pathogen-associated molecular pattern, was specifically recognized by the Toll-like receptor 2, which, in turn, upregulated the expression levels of proteins downstream of the mitogen-activated protein kinase and nuclear factor κB pathways. Notably, the immune activity of the F2 fraction was markedly higher than that of the crude polysaccharides. In summary, the purified F2 fraction of SHP may be an effective nutritional supplement for human disorders associated with low immunity.

The mechanisms underlying melatonin improved soybean seedling growth at different nitrogen levels.

To investigate the function of melatonin (MT) on nitrogen uptake and metabolism in soybean, six groups of treatments, with and without 100µM melatonin were conducted at low, normal, and high nitrogen levels (1.5, 7.5, and 15mM, respectively). The related indexes of nitrogen metabolism and the antioxidant system of seedlings were measured and analysed. Results indicated that MT could enhance the level of nitrogen metabolism by upregulating the coding genes of enzymes related to nitrogen metabolism and increasing total nitrogen content, especially under low nitrogen levels. Under high nitrogen conditions, the addition of MT not only accelerated ammonium assimilation and utilisation by enhancing the activity of glutamine synthetase involved in ammonium assimilation, but also reduced the extent of membrane lipid peroxidation to alleviate the degree of damage by improving the activity of antioxidant enzymes. In addition, MT enhanced soybean growth with positive effects in morphological changes at different nitrogen levels, including significantly increased stem diameter, total leaf area, and root nodule number, and biomass accumulation. Finally, biomass accumulation increased under low, normal, and high nitrogen levels by 9.80%, 14.06%, and 11.44%, respectively. The results suggested that MT could enhance the soybean tolerance to low and excessive N treatments.

Exogenous melatonin reduces the inhibitory effect of osmotic stress on antioxidant properties and cell ultrastructure at germination stage of soybean.

Understanding the relationship between exogenous melatonin and water deficit stress is crucial for alleviating the effects of water deficit stress at germination stage of soybean (Glycine max (L.) Merrill) in agriculture. This study investigated the effects of exogenous melatonin on soybean antioxidant properties and cell ultrastructure under water deficit stress induced by polyethylene glycol (PEG) 6000. The drought-sensitive soybean variety Suinong 26 was used as the material to study the effects of different concentrations of melatonin (0, 300, 500 µmol·L-1) soaking soybean seeds under drought stress (PEG-6000: 3% and 6%). The results showed that the germination rate (GR), germination potential (GP), germination index (GI) and radicle shape of soybean were affected negatively to different degrees under PEG stress. Moreover, stress induced by different PEG concentrations overproduced the content of reactive oxygen species (H2O2, O2·-) in cells, leading to increased lipid membrane peroxidation as electrolyte leakage (EL) and malondialdehyde (MDA) content, which resulted in impaired cell integrity. However, after seeds soaking with melatonin, the lipid peroxidation of the cell membrane was reduced, and the activities of antioxidant enzymes such as superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX) further increased to minimize the excessive generation of ROS. Similar results were obtained for soluble protein and proline, that may help in regulating the osmotic pressure and maintain cellular integrity. With the interaction of these enzymes, compared with 300 µmol·L-1 melatonin, 500 µmol·L-1 melatonin could more effective to remove the ROS and reduce cell peroxidation. Overall, 500 µmol·L-1 melatonin performed better than 300 µmol·L-1. In conclusion, the seed soaking with melatonin promoted the germination of soybean seeds under water stress.

Transcriptomic and metabolomic profiling of melatonin treated soybean (Glycine max L.) under drought stress during grain filling period through regulation of secondary metabolite biosynthesis pathways.

There is a growing need to enhance the productivity of soybean (Glycine max L.) under severe drought conditions in order to improve global food security status. Melatonin, a ubiquitous hormone, could alleviate drought stress in various plants. Earlier, we demonstrated that exogenous melatonin treatment could enhance the tolerance of drought-treated soybean. However, the underlying mechanisms by which this hormone exerts drought resistance is still unclear. The present study used transcriptomic and metabolomic techniques to determine some critical genes and pathways regulating melatonin response to drought conditions. Results showed that exogenous melatonin treatment could increase relative water content and decrease electrolyte leakage in the leaves and increase seed yield under drought stress. Transcriptomic analysis showed that there were 852 core differentially expressed genes (DEGs) that were regulated by drought stress and melatonin in soybean leaves. The most enriched drought-responsive genes are mainly involved in the 'biosynthesis of secondary metabolites'. Metabolomic profiling under drought stress showed higher accumulation levels of secondary metabolites related to drought tolerance after exogenous melatonin treatment. Also, we highlighted the vital role of the pathways including phenylpropanoid, flavonoid, isoflavonoid, and steroid biosynthesis pathways for improvement of drought tolerance in soybean by exogenous melatonin treatment. In all, findings from this study give detailed molecular basis for the application of melatonin as a drought-resistant agent in soybean cultivation.

Exogenous melatonin reduces the inhibitory effect of osmotic stress on photosynthesis in soybean.

Understanding the relationship between exogenous melatonin and water deficit stress is crucial for achieving high yields and alleviating the effects of water deficit stress on soybean (Glycine max (L.) Merrill) plants in agriculture. This study investigated the effects of exogenous melatonin on soybean photosynthetic capacity under water deficit stress induced by polyethylene glycol (PEG) 6000. We conducted a potting experiment in 2018 using the soybean (Glycine max L. Merrill) cultivar Suinong 26. We identified the impacts of a concentration of PEG 6000 simulating drought (15%, w/v) and an appropriate melatonin concentration (100 µmol/L) on the growth of soybean seedlings and flowering stages in a preliminary test. We applied exogenous melatonin by foliar spraying and root application to determine the effects on leaf photosynthesis during water deficit stress. Our results indicated that 15% PEG 6000 had an obvious inhibitory effect on the growth of soybean seedlings and flowering stages, causing oxidative stress and damage due to reactive oxygen species (ROS) (H2O2 and O2·-) accumulation and potentially reducing air exchange parameters and photosystem II (PSII) efficiency. The application of exogenous melatonin significantly relieved the inhibitory effects of PEG 6000 stress on seedlings and flowering growth, and gas exchange parameters, potentially improved PSII efficiency, improved the leaf area index (LAI) and the accumulation of dry matter, slowed down oxidative stress and damage to leaves by increasing the activity of antioxidant enzymes (SOD, POD, and CAT), reduced the content of malondialdehyde (MDA), and ultimately improved soybean yield. Overall, the results of this study demonstrated that application of exogenous melatonin at the seedlings and flowering stages of soybean is effective in alleviating plant damage caused by water deficit stress and improving the drought resistance of soybean plants. In addition, the results showed that application of exogenous melatonin by root is superior to foliar spraying.

miR-134 functions as a tumor suppressor in cell proliferation and epithelial-to-mesenchymal Transition by targeting KRAS in renal cell carcinoma cells.

Aberrant microRNAs (miRNAs) are reported to contribute to the pathogenesis of most human malignancies. The miRNA, miR-134, has been found to be downregulated in renal cell carcinoma (RCC), but its function in the disease is unknown. The aims of this study were to detect the expression of miR-134 in human RCC samples and explore its function in RCC cell lines. Real-time qualitative polymerase chain reaction (qPCR) was used to quantify miR-134 in human RCC samples. Assays for cell cycle, viability, migration, and invasion were performed to assess the phenotypic changes in RCC cells. A luciferase reporter assay was carried out to confirm whether KRAS (Kirsten rat sarcoma viral oncogene homolog) is a direct target of miR-134. Western blot was used to identify the potential signaling pathways that had an impact on RCC cell growth and alterations of markers for epithelial-mesenchymal transition (EMT), which affected metastasis by miR-134. miR-134 was found to be downregulated in RCC samples (p<0.05), while overexpression of miR-134 suppressed proliferation (p<0.05) by triggering G1/G0 cell cycle arrest (p<0.05). Forced expression of miR-134 could also inhibit migration (p<0.05) and invasion (p<0.05) by blocking EMT in RCC cell lines. KRAS was identified as a target of miR-134, and miR-134 may act as a tumor suppressor through the KRAS-related MAPK/ERK pathway other than PI3K/AKT signaling. Thus, miR-134 may function as a tumor suppressor in cell proliferation and EMT by targeting KRAS in RCC cells.

Genetic variants in RKIP are associated with clear cell renal cell carcinoma risk in a Chinese population.

BACKGROUND: Raf-1 kinase inhibitor protein (RKIP) plays a critical role in tumor development by regulating cell functions such as invasion, apoptosis and differentiation. Down-regulation of RKIP expression has been implicated in the development and progression of renal cell carcinoma (RCC). Herein, we hypothesized that genetic polymorphisms in RKIP might be associated with susceptibility and progression of RCC. METHODS: A total of 5 tagging single-nucleotide polymorphisms (tSNPs) in RKIP were selected and genotyped by SNapShot method in a case-control study of 859 RCC patients and 1004 controls. The logistic regression was used to evaluate the genetic association with occurrence and progression of RCC. The functionality of the important SNP was preliminary examined by qRT-PCR. RESULT: We found that the rs17512051 in the promoter region of RKIP was significantly associated with decreased clear cell RCC (ccRCC) risk (TA/AA vs. TT: P = 0.039, OR = 0.78, 95%CI = 0.62-0.99). Another SNP (rs1051470) in the 3'UTR region of RKIP was marginally associated with increased ccRCC risk (TT vs. CC+CT: OR = 1.45, 95%CI = 1.01-2.09). In the stratified analysis, the protective effect of rs17512051 was more predominant in the subgroups of male, non-smokers, non-drinkers as well as subjects without history of diabetes. Furthermore, we observed higher RKIP mRNA levels in the presence of the rs17512051A allele in normal renal tissues. CONCLUSION: Our results suggest that the potentially functional RKIP rs17512051 polymorphism may affect ccRCC susceptibility through altering the endogenous RKIP expression level. Risk effects and the functional impact of this polymorphism need further validation.