RESUMEN
A novel cell line, Epinephelus moara kidney cell line (EMK), was established from kidneys of kelp grouper E. moara. Cells were cultured at 24 °C in Leibovitz's L-15 medium (L15) supplemented with antibiotics, basic fibroblast growth factor (bFGF), foetal bovine serum (FBS) and 2-mercaptoethanol (2-ME). EMK cells, fibroblastic in morphology, proliferated to 100% confluency in 3-4 days and were subcultured for over 50 passages. The cells could grow from 18 to 30 °C, with optimal growth at 24 °C. Chromosome analysis indicated that the modal chromosome number was 48 in the cells at passage 42. Green fluorescent signals could be observed in EMK cells when the cells were transfected with pEGFP-N3 plasmid. Moreover, a significant cytopathic effect (CPE) was observed in the cells after infection with Singapore grouper iridovirus (SGIV) or nervous necrosis virus (NNV), and viral replication was confirmed by quantitative real-time PCR (qPCR). These results suggested the potential of the EMK cell line for studies of transgene and pathogenesis of SGIV and NNV.
Asunto(s)
Gadiformes/fisiología , Riñón/citología , Virus/clasificación , Animales , Técnicas de Cultivo de Célula/veterinaria , Línea Celular , Iridovirus/fisiología , Nodaviridae/fisiología , Temperatura , Replicación Viral/fisiologíaRESUMEN
OBJECTIVE: To study the reasons and possibly solutions for missed diagnosis of duodenal injury. METHOD The clinical records were retrospectively analyzed in 9 cases of missed diagnosis of duodenal injury treated in our hospital in the year 1992 to 2002. RESULTS Of the 15 cases of duodenal injury, missed diagnoses occurred in 9 cases. The reasons for the missed diagnoses included complications by injuries of the other abdominal organs that concealed duodenal injuries, neglect of the light injuries, and the lack of clinical experience on the part of the young doctors. CONCLUSION Duodenal injury frequently occurs along with other abdominal organ injuries and is easy to escape detection, which can be avoided by careful preoperative examination of the characteristics of injuries and the patients' clinical manifestations after the injury.
