Safety evaluation method in multi-logical scenarios for automated vehicles based on naturalistic driving trajectory.

Automated driving technology has constantly been maturing; however, how to ensure automated vehicle (AV) safety has not yet been effectively solved, functional safety assessment remains an important part of the development of automated driving technology. To compensate for the lack of multidimensional evaluation indicators, this paper proposes a safety evaluation method in multi-logical scenarios (SEMMS) for AVs' functional safety based on naturalistic driving trajectory (NDT) in order to evaluate the comprehensive performance of the tested AV in a diversity of scenarios simultaneously. The potential field method is used to describe the quantified danger level of an AV in a single concrete scenario that considers the dangerous situation of the scenario and AV test results. Combined with the internal probability distribution of the logical scenario parameter space obtained by NDT, the safety performance of an AV in logical scenario is calculated by integrating the two indexes. With the information entropy and relative frequency of different logical scenarios, the relative weights of logical scenarios are obtained, and the safety performance evaluation results of the tested AV in the multi-logical scenarios can be determined based on the weighting danger level in different logical scenarios. During the actual application of the method, the HighD database was used as the input source of NDT, and a black-box automated driving algorithm was subjected to traversal tests in three logical scenarios. The test results of the automated driving algorithm were evaluated using the SEMMS, and the results show that the SEMMS could well evaluate the performance of the tested automated driving algorithm in multiple kinds of logical scenarios simultaneously, indicating that it is an effective solution to the problem of automated driving algorithm safety evaluation.

In vitro activity and adaptation strategies of eravacycline in clinical Enterococcus faecium isolates from China.

Eravacycline (Erava) is a synthetic fluorocycline with potent antimicrobial activity against a wide range of Gram-positive bacteria. This study aimed to investigate the in vitro antimicrobial activity and resistance mechanism of Erava in clinical E. faecium isolates from China. Erava minimum inhibitory concentrations (MICs) against clinical E. faecium isolates-including those resistant to linezolid (LZD) or harboring the tetracycline (Tet) resistance genes was ≤0.25 mg l-1. Moreover, our data indicated that clinical isolates of E. faecium with Erava MIC 0.25 mg l-1 were predominantly shown to belong to Sequence-type 78 (ST78) and ST80. The prevalence of Erava heteroresistance in clinical E. faecium strain was 2.46% (3/122). The increased Erava MIC values of heteroresistance-derived E. faecium clones could be significantly reduced by efflux pump inhibitors (EPIs). Furthermore, comparative proteomics results showed that efflux pumps lmrA, mdlA, and mdlB contributed significantly to the acquisition of Erava resistance in E. faecium. In addition, a genetic mutation in 16 S rRNA (G190A) were detected in resistant E. faecium isolates induced by Erava. In summary, Erava exhibits potent in vitro antimicrobial activity against E. faecium, but mutation of Tet target sites and elevated expression of efflux pumps under Erava selection results in Erava resistance.