Asunto(s)
Humanos , Neoplasias/diagnóstico , Nucleósidos/orina , Biomarcadores de Tumor/orina , Neoplasias/diagnóstico , Neoplasias/orina , Nucleósidos/diagnóstico , Nucleósidos/aislamiento & purificación , Electroforesis Capilar , Biomarcadores de Tumor/diagnóstico , Neoplasias/orina , Técnicas de Laboratorio ClínicoRESUMEN
Thirteen normal and modified nucleosides, primarily degradation products of transfer ribonucleic acid (tRNA), were evaluated as potential tumor markers for cancer patients. Their urinary concentrations were determined by means of micellar electrokinetic capillary chromatography (MEKC) in the urine from 54 healthy adults and 70 cancer patients, then quantitatively expressed as a function of creatinine excretion. It was found that urinary nucleosides for cancer patients were on the average significantly higher than those for healthy controls, however, no significant differences were found between male and female or between different ages. Based on 13 urinary nucleoside concentrations, principal component analysis (PCA) could be used to classify 72% of cancer patients from the healthy controls. The present study shows that the precise measurement of urinary nucleosides by MEKC in combining with PCA technique may provide a clinically useful approach for diagnosis of cancer.
Asunto(s)
Neoplasias/orina , Nucleósidos/orina , Adulto , Biomarcadores/orina , Tampones (Química) , China , Electroforesis Capilar/métodos , Humanos , Indicadores y Reactivos , Micelas , Persona de Mediana Edad , Valores de Referencia , Reproducibilidad de los ResultadosRESUMEN
A capillary electrophoretic method for determining the inorganic anions in the ash of black powder has been developed. The components and pH of the buffer solution, the concentration of osmotic flow modifier and the separation voltage were investigated. The optimized parameters were 5.0 mmol/L sodium chromate buffer solution (pH 8.20) containing 0.5 mmol/L cetyltrimethylammonium bromide (CTAB) as osmotic flow modifier, a separation voltage of -20 kV and a detection wavelength of 254 nm. Under the specified conditions five anions were completely separated in four minutes, and the repeatabilities (RSD) of migration time and peak area were in the range of 0.17%-1.4% and 3.9%-5.0%, respectively. The detection limits were in the range of 5.0 mumol/L to 10.0 mumol/L. To show the usefulness, the method was applied to analyze the ash of a black powder and the results showed that the relative standard deviations of the determination for Cl- and NO2- were 6.0% and 3.9%, respectively.