RESUMEN
As a natural active substance that can effectively improve blood lipid balance in the body, hypolipidemic active peptides have attracted the attention of scholars. In this study, the effect of walnut meal peptides (WMP) on lipid metabolism was investigated in rats fed a high-fat diet (HFD). The experimental results show that feeding walnut meal peptides counteracted the high-fat diet-induced increase in body, liver and epididymal fat weight, and reduce the serum concentrations of total cholesterol, triglycerides, and LDL-cholesterol and hepatic cholesterol and triglyceride content. Walnut meal peptides also resulted in increased HDL-cholesterol while reducing the atherosclerosis index (AI). Additionally, the stained pathological sections of the liver showed that the walnut meal peptides reduced hepatic steatosis and damage caused by HFD. Furthermore, walnut meal peptide supplementation was associated with normalization of elevated apolipoprotein (Apo)-B and reduced Apo-A1 induced by the high-fat diet and with favorable changes in the expression of genes related to lipid metabolism (LCAT, CYP7A1, HMGR, FAS). The results indicate that walnut meal peptides can effectively prevent the harmful effects of a high-fat diet on body weight, lipid metabolism and liver fat content in rats, and provide, and provide a reference for the further development of walnut meal functional foods.
Asunto(s)
Dieta Alta en Grasa , Hiperlipidemias/tratamiento farmacológico , Juglans/química , Metabolismo de los Lípidos , Hígado/metabolismo , Péptidos/uso terapéutico , Adipocitos/efectos de los fármacos , Adipocitos/patología , Aminoácidos/análisis , Animales , Apolipoproteínas/metabolismo , Peso Corporal/efectos de los fármacos , Ciego/efectos de los fármacos , Ciego/patología , Colesterol/metabolismo , Ingestión de Energía/efectos de los fármacos , Epidídimo/efectos de los fármacos , Epidídimo/patología , Regulación de la Expresión Génica/efectos de los fármacos , Hidrólisis , Hipolipemiantes/farmacología , Hipolipemiantes/uso terapéutico , Metabolismo de los Lípidos/efectos de los fármacos , Hígado/efectos de los fármacos , Hígado/enzimología , Hígado/patología , Masculino , Tamaño de los Órganos/efectos de los fármacos , Péptido Hidrolasas/metabolismo , Péptidos/farmacología , Ratas Sprague-DawleyRESUMEN
The phenolic composition of the free phenolic extract from Sphallerocarpus gracilis seeds was analyzed by HPLC-MS and predominant compounds were chlorogenic acid, di-caffeoylquinic acid glucoside and luteolin-7-O-glucoside. The free phenolic extract was evaluated for DNA damage protective activity induced by ROO and OH radicals and hepatoprotective effect in vivo and in vitro. Results revealed that the free phenolic extract exhibited significant protective activity against both ROO and OH radical-induced DNA damage and the phenolic extract exerted more potent inhibitory activity against OH radical-induced damage than against that induced by ROO radicals. In vivo experimental results showed that the phenolic extract significantly prevented the increase of serum alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase activities and hepatic malondialdehyde level caused by CCl4 in rats, and markedly increased hepatic superoxide dismutase, catalase and glutathione peroxidase levels. Histopathological examinations further confirmed that the phenolic extract could protect the liver from CCl4-induced damage. In vitro experimental results showed that the phenolic extract could reduce BRL hepatocyte apoptosis and damage induced by CCl4. These findings indicate that the S. gracilis seed could be developed as a medicinal herb for the therapy and prevention of hepatic injury.