Type-II quantum spin Hall effect in two-dimensional metals.

The quantum spin Hall (QSH) effect has been observed in topological insulators and long quantum wells using spin-orbit coupling as the probe, but it has not yet been observed in a metal. An experiment is proposed to measure the different Type-II QSH effect of an electron or hole in a two-dimensional (2D) metal by using the previously unexplored but relativistically gauge-invariant form of the generated 2D QSH Hamiltonian. Instead of using the electric field in the surface of the spin-polarized bands of a topological insulator or across the quantum well width as the probe, ones uses an applied azimuthal vector potential and an applied radial electric field as the tools to generate a spontaneously quantized spin current in an otherwise spin unpolarized 2D metal. A long cylindrical solenoid lies normally through the inner radius of a 2D metallic Corbino disk. The currentISsurrounding the solenoid produces an azimuthal magnetic vector potential but no magnetic field in the disk. In addition, a radial electric field is generated across the disk by imposing either a potential differenceΔvor a radial charge currentIacross its inner and outer radii. Combined changes inISand in eitherΔvorIgenerate spontaneously quantized azimuthal charge and spin currents. The experiment is designed to measure these quantized azimuthal charge and spin currents in the disk consistently. The quantum Hamiltonians for both experiments are solved exactly. A method to control the Joule heating is presented, which could potentially allow the Type-II QSH measurements to be made at room temperature.

Angular dependence of the upper critical induction of cleans- anddx2-y2-wave superconductors with self-consistent ellipsoidal effective mass and Zeeman anisotropies.

We employ the Schrödinger-Dirac method generalized to an ellipsoidal effective mass anisotropy in order to treat the spin and orbital effective mass anisotropies self consistently, which is important when Pauli-limiting effects on the upper critical field characteristic of singlet superconductivity are present. By employing the Klemm-Clem transformations to map the equations of motion into isotropic form, we then calculate the upper critical magnetic inductionBc2(θ,ϕ,T)at arbitrary directions and temperaturesTfor isotropics-wave and for anisotropicdx2-y2-wave superconducting order parameters. As for anisotropics-wave superconductors,Bc2is largest in the direction of the lowest effective mass, and is proportional to the universal orientation factorα(θ,ϕ). However, fordx2-y2-wave pairing and vanishing planar effective mass anisotropy,Bc2(π/2,ϕ,T)exhibits a four-fold azimuthal pattern withC4symmetry the maxima of which are along the crystal axes just below the transition temperatureTc, but these maxima are rotated byπ/4about thezaxis asTis lowered to 0. However fordx2-y2-wave pairing with weak planar effective mass anisotropy,Bc2(π/2,ϕ,T)exhibits a two-fold pattern withC2symmetry for allT ⩽ Tc, which also rotates byπ/4about thezaxis asTis lowered to 0. These low planar effective mass anisotropy cases provide a new method to distinguishs-wave anddx2-y2-wave pairing symmetries in clean unconventional superconductors.

The Zeeman, spin-orbit, and quantum spin Hall interactions in anisotropic and low-dimensional conductors.

To construct a microscopic theory of electrons and holes in anisotropic conductors that self-consistently treats their band effective mass anisotropy with their interactions with applied electric and magnetic fields, the Dirac equation is extended for an electron or hole in an orthorhombically-anisotropic conduction band. Its covariance is established both by a modified version of the Klemm-Clem transformations to a space in which it is isotropic, and also in its fully anisotropic form by making the most general proper and improper Lorentz transformations, proving its validity in both the relativistic and non-relativistic limits. The appropriate Foldy-Wouthuysen transformations are extended to expand about the non-relativistic Hamiltonian limit to fourth order in the inverse of the particle's Einstein rest energy. The results have important consequences for magnetic measurements of many classes of clean anisotropic semiconductors, metals, and superconductors. In all of these cases, the Zeeman interaction is found to depend strongly upon the effective mass anisotropy. When an electron or hole is traveling in an atomically thin one-dimensional conduction band, its Zeeman, spin-orbit, and quantum spin Hall interactions are vanishingly small. Accurate expressions for the Zeeman, spin-orbit and quantum spin Hall interactions for two-dimensional conductors are provided.

Baicalin relieves inflammation stimulated by lipopolysaccharide via upregulating TUG1 in liver cells.

Hepatitis has become a major social, health, and economic problem worldwide. Herein, we tested the beneficial influence of baicalin, a flavonoid extracted from the roots of Scutellaria baicalensis, on human normal liver L-02 and THLE2 cell apoptosis and inflammatory reaction stimulated by lipopolysaccharide (LPS) and possible molecular mechanisms. L-02 and THLE2 cell viability and apoptosis after LPS and/or baicalin treatment were tested using CCK-8 assay and Annexin V-FITC/PI apoptosis kit, respectively. qRT-PCR was used to measure the MCP-1, IL-6, TNF-α, and lncRNA taurine upregulated gene 1 (TUG1) expressions in L-02 and THLE2 cells. sh-TUG1 was transfected to knockdown TUG1. SB203580 was used as inhibitor of p38MAPK pathway, while SP600125 was used as inhibitor of JNK pathway. We discovered that LPS stimulation caused L-02 and THLE2 cell apoptosis and inflammatory reaction. Baicalin relieved the L-02 and THLE2 cell apoptosis and inflammatory reaction stimulated by LPS. Moreover, LPS lowered the TUG1 expression in L-02 cells, while baicalin promoted the TUG1 expression in L-02 and L-02 and THLE2 cells, as well as inactivated p38MAPK and JNK pathways in LPS-stimulated L-02 cells. Besides, knockdown of TUG1 activated p38MAPK and JNK pathways and promoted inflammatory cytokine expression in L-02 cells. In conclusion, this study further affirmed the beneficial influences of baicalin on LPS-stimulated human normal liver cell apoptosis and inflammatory reaction. Baicalin relived liver cell inflammation stimulated by LPS might be via upregulating TUG1 and then inactivating p38MAPK and JNK pathways.

Theoretical prediction of induction period from transient pore evolvement in polyester-based microparticles.

A model was developed and compared to experimental results for prediction of the induction period during drug delivery from various compositions of biodegradable copolymer PLGA microparticles. The uniqueness of this model is that it considers transient pore evolvement and uses the kinetic parameters of polymer degradation, which are independent of experimental measurements of microparticle erosion, in its analysis. Delivery data from PLGA microparticles (50:50, 75:25, and 85:15) releasing ovalbumin (OVA, 46 kDa) and bovine serum albumin (BSA, 66 kDa) were determined and used as the model systems. Experimental measurements were carried out from 85 to 150 days depending on the PLGA characteristics. The predicted induction periods were approximately 45, 70, and 105 days for the release of both OVA and BSA from 50:50, 75:25, and 85:15 PLGA microparticles, respectively. Overall, these values were in very good agreement with experimentally estimated results.

Using TEM to couple transient protein distribution and release for PLGA microparticles for potential use as vaccine delivery vehicles.

In the development of tunable PLGA microparticles as vaccine delivery vehicles, it is important to understand the drug distribution within the microparticle over time as well as the long-term release of the drug during polymer degradation. This study addresses the transient 3-D drug distribution in PLGA microparticles during in vitro degradation. Specifically, poly (lactide-co-glycolide) (PLGA 75:25) microparticles containing ovalbumin (OVA) as a model protein were fabricated by double-emulsion (w/o/w) method. The microparticles were incubated at 37 degrees C and 250 rpm in PBS buffer (pH 7.4) over a 100-day period. The in vitro polymer erosion, transient protein distribution profiles and protein release behaviors were investigated. Protein release profiles were determined via spectrophotometry using a BCA assay for the solution. Transmission electron microscopy (TEM) images were obtained for the OVA-loaded microparticles before and during degradation (0 day, 30 days and 60 days), and the corresponding 3-D constructions were developed. From the 3-D constructions, the overall protein distribution of the entire microparticle was vividly reflected. Pixel number analysis of the TEM images was used to quantify transient protein distribution. The transient protein release obtained from the TEM analysis was in good agreement with the BCA analysis. This technique provides an additional tool in helping develop polymer matrices for tunable delivery vehicles in vaccination and other drug delivery scenarios.