Pararcticibacter amylolyticus gen. nov., sp. nov., Isolated from a Rotten Hemp Rope, and Reclassification of Pedobacter tournemirensis as Pararcticibacter tournemirensis comb. nov.

A Gram-stain-negative, rod-shaped, non-motile, facultatively anaerobic bacterium, designated FJ4-8T, was isolated from a rotten hemp rope in Chongqing City, PR China. Phylogenetic analysis of 16S rRNA gene sequences indicated that the isolate was closely related to members of the family Sphingobacteriaceae, with the highest similarity to Pedobacter tournemirensis TF5-37.2-LB10T (95.3%) and low similarities to all other species of the genus Pedobacter (90.4-93.9%). Phylogenetic analyses demonstrated that strain FJ4-8T formed a stable subclade with Pedobacter tournemirensis TF5-37.2-LB10T. The clade with these two strains branched adjacent to a clade containing three species of the genus Arcticibacter. MK-7 was detected as the only respiratory quinone. The major fatty acids composed iso-C15:0, iso-C17:0 3-OH and summed feature three. Phosphatidylethanolamine, three aminophospholipids and one unidentified lipid were found as the major polar lipids. The major polyamine was identified as sym-homospermidine. The DNA-DNA hybridization value between strain FJ4-8T and Pedobacter tournemirensis TF5-37.2-LB10T was 42.0 ± 2.5%. Based on its phylogenetic, chemotaxonomic and phenotypic characteristics, the novel strain and TF5-37.2-LB10T were found to be different from members of genera Pedobacter and Arcticibacter. FJ4-8T and TF5-37.2-LB10T represented different species. Therefore, FJ4-8T should be classified as a novel species of a novel genus in the family Sphingobacteriaceae, for which the name Pararcticibacter amylolyticus gen. nov., sp. nov. is proposed. The type strain is FJ4-8T (= KCTC 62640T = CCTCC AB 2018052T). The draft genome sequence is 6290, 449 bp in length, the genomic DNA G+C content was 44.4 mol%. Pedobacter tournemirensis TF5-37.2-LB10T should be transferred to the novel genus as Pararcticibacter tournemirensis comb. nov. (The type strain is TF5-37.2-LB10T (= DSM 23085T = CIP 110085T = MOLA 820T).

Chitinophaga salinisoli sp. nov., isolated from saline soil.

A novel aerobic bacterium, designated strain LAM9153T, was isolated from a saline soil sample collected from Lingxian County, Shandong Province, China. Cells of strain LAM9153T were observed to be Gram-stain negative, non-motile, non-spore-forming and rod-shaped. The new isolate grew optimally at 30-35 °C, pH 7.0 and 0.5% of NaCl concentration (w/v). According to the phylogenetic analysis based on the 16S rRNA gene sequence, strain LAM9153T shares high similarity with Chitinophaga terrae Gsoil 238T (96.9%) and Chitinophaga niabensis JS 13-10T (95.9%), forming a subcluster with C. terrae Gsoil 238T, Chitinophaga cymbidii R156-2T, C. niabensis JS 13-10T and Chitinophaga soli Gsoil 219T in the phylogenetic tree. The major cellular fatty acids (> 10%) were identified as iso-C15:0, iso-C17:0 3-OH and summed features 3 (C16:1 ω6c and/or C16:1 ω7c). The predominant respiratory quinone was identified as menaquinone MK-7. The polar lipids consisted of phosphatidylethanolamine, aminophospholipid, three unidentified aminolipids and five unidentified lipids. The genomic DNA G+C content was determined to be 53.2 ± 1.6 mol%. On the basis of phylogenetic, chemotaxonomic and phenotypic data, strain LAM9153T is concluded to represent a novel species of the genus Chitinophaga, for which the name Chitinophaga salinisoli sp. nov. is proposed. The type strain is LAM9153T (= ACCC 19960T = JCM 30847T).

Arenimonas alkanexedens sp. nov., isolated from a frozen soil sample.

A novel facultatively anaerobic bacterium, designated strain LAM-WHM-D11T, was isolated from a frozen soil sample of China. Cells of strain LAM-WHM-D11T were observed to be Gram-stain negative, non-motile and rod-shaped. Colonies were yellowish, and circular with convex shape. Strain LAM-WHM-D11T was found to be able to grow at 4-40 °C (optimum 15 °C), pH 7.5-2.0 (optimum 9.5) and 0-2.5% NaCl (w/v) (optimum 1.5%). The 16S rRNA gene sequence similarity analysis showed that strain LAM-WHM-D11T is closely related to Arenimonas metalli CF5-1T (98.0%), Arenimonas aquaticum NA-09T (97.9%), Arenimonas donghaensis HO3-R19T (95.6%) and Arenimonas aestuarii S2-21T (95.3%). The DNA-DNA hybridization values between the isolate and A. metalli CGMCC 1.10787T, A. aquaticum KACC 14663T, A. donghaensis KACC 11381T were 41.0 ± 1.7, 44.7 ± 1.4 and 42.8 ± 1.2%, respectively. The genomic DNA G+C content was found to be 66.5 mol% as determined by the T m method. The major cellular fatty acids were identified as iso-C15:0 and iso-C16:0. The major isoprenoid quinone was identified as ubiquinone 8 (Q-8). The major polar lipids were found to be diphosphatidyglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylmonomethylethanolamine, two phospholipids and five unidentified lipids. Based on the phylogenetic, phenotypic and chemotaxonomic characteristics, strain LAM-WHM-D11T is concluded to represent a novel species within the genus Arenimonas, for which the name Arenimonas alkanexedens sp. nov. is proposed. The type strain is LAM-WHM-D11T (ACCC 19750T = JCM 30464T).