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1.
Braz Oral Res ; 33: e117, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-31939498

RESUMEN

The aim of this study was to evaluate the effect of mineral trioxide aggregate (MTA) and Brazilian propolis on the cell viability, mineralization, anti-inflammatory ability, and migration of human dental pulp cells (hDPCs). The cell viability was evaluated with CCK-8 kit after 1, 5, 7, and 9 days. The deposition of calcified matrix and the expression of osteogenesis-related genes were evaluated by Alizarin Red staining and real-time PCR after incubation in osteogenic medium for 21 days. The expression of inflammation-related genes in cells was determined after exposure to 1 µg/mL LPS for 3 h. Finally, the numbers of cells that migrated through the permeable membranes were compared during 15 h. Propolis and MTA significantly increased the viability of hDPCscompared to the control group on days 7 and 9. In the propolis group, significant enhancement of osteogenic potential and suppressed expression of IL-1ß and IL-6 was observed after LPS exposure compared to the MTA and control groups. The number of migration cells in the propolis group was similar to that of the control group, while MTA significantly promoted cell migration. Propolis showed comparable cell viability to that of MTA and exhibited significantly higher anti-inflammatory and mineralization promotion effects on hDPCs.


Asunto(s)
Compuestos de Aluminio/farmacología , Antiinflamatorios/farmacología , Compuestos de Calcio/farmacología , Pulpa Dental/citología , Pulpa Dental/efectos de los fármacos , Óxidos/farmacología , Própolis/farmacología , Silicatos/farmacología , Antraquinonas , Brasil , Movimiento Celular/efectos de los fármacos , Supervivencia Celular/efectos de los fármacos , Células Cultivadas , Combinación de Medicamentos , Humanos , Interleucina-1beta/análisis , Interleucina-6/análisis , Odontoblastos/efectos de los fármacos , Reacción en Cadena en Tiempo Real de la Polimerasa , Reproducibilidad de los Resultados , Estadísticas no Paramétricas , Factor de Necrosis Tumoral alfa/análisis
2.
Braz. oral res. (Online) ; 33: e117, 2019. tab, graf
Artículo en Inglés | LILACS, BBO - Odontología | ID: biblio-1132651

RESUMEN

Abstract: The aim of this study was to evaluate the effect of mineral trioxide aggregate (MTA) and Brazilian propolis on the cell viability, mineralization, anti-inflammatory ability, and migration of human dental pulp cells (hDPCs). The cell viability was evaluated with CCK-8 kit after 1, 5, 7, and 9 days. The deposition of calcified matrix and the expression of osteogenesis-related genes were evaluated by Alizarin Red staining and real-time PCR after incubation in osteogenic medium for 21 days. The expression of inflammation-related genes in cells was determined after exposure to 1 μg/mL LPS for 3 h. Finally, the numbers of cells that migrated through the permeable membranes were compared during 15 h. Propolis and MTA significantly increased the viability of hDPCscompared to the control group on days 7 and 9. In the propolis group, significant enhancement of osteogenic potential and suppressed expression of IL-1β and IL-6 was observed after LPS exposure compared to the MTA and control groups. The number of migration cells in the propolis group was similar to that of the control group, while MTA significantly promoted cell migration. Propolis showed comparable cell viability to that of MTA and exhibited significantly higher anti-inflammatory and mineralization promotion effects on hDPCs.


Asunto(s)
Humanos , Óxidos/farmacología , Própolis/farmacología , Silicatos/farmacología , Compuestos de Calcio/farmacología , Compuestos de Aluminio/farmacología , Pulpa Dental/citología , Pulpa Dental/efectos de los fármacos , Antiinflamatorios/farmacología , Brasil , Movimiento Celular/efectos de los fármacos , Supervivencia Celular/efectos de los fármacos , Células Cultivadas , Reproducibilidad de los Resultados , Antraquinonas , Interleucina-6/análisis , Factor de Necrosis Tumoral alfa , Estadísticas no Paramétricas , Combinación de Medicamentos , Interleucina-1beta/análisis , Reacción en Cadena en Tiempo Real de la Polimerasa , Odontoblastos/efectos de los fármacos
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