RESUMEN
Hepatocellular carcinoma is a common malignant tumor with high recurrence rate. Long non-coding RNA (lncRNA) ZNF561 antisense RNA 1 (ZNF561-AS1) functions as an oncogenic lncRNA to promote the tumorigenesis of colorectal cancer. The role of ZNF561-AS1 in hepatocellular carcinoma remains unknown. ZNF561-AS1 was elevated in hepatocellular carcinoma tissues and cells. Silence of ZNF561-AS1 reduced cell viability and inhibited the proliferation of hepatocellular carcinoma. The angiogenesis of hepatocellular carcinoma was also suppressed by loss of ZNF561-AS1 with a decrease of angiopoietin 2, fibroblast growth factor 1, and vascular endothelial growth factor. ZNF561-AS1 bind to miR-302a-3p, and decreased expression of miR-302a-3p in hepatocellular carcinoma. Moreover, miR-302a-3p reduced platelet-derived growth factor-D (PDGFD) in hepatocellular carcinoma, and inhibition of miR-302a-3p attenuated ZNF561-AS1 silence-induced decrease of PDGFD. In conclusion, silence of ZNF561-AS1 might inhibit cell proliferation and angiogenesis of hepatocellular carcinoma through downregulation of miR-302a-3p-mediated PDGFD.
Asunto(s)
Carcinoma Hepatocelular , Neoplasias Hepáticas , MicroARNs , ARN Largo no Codificante , Humanos , Carcinoma Hepatocelular/genética , Carcinoma Hepatocelular/metabolismo , ARN sin Sentido/genética , ARN Largo no Codificante/genética , ARN Largo no Codificante/metabolismo , Regulación hacia Arriba , MicroARNs/genética , MicroARNs/metabolismo , Regulación Neoplásica de la Expresión Génica , Factor A de Crecimiento Endotelial Vascular/genética , Factor A de Crecimiento Endotelial Vascular/metabolismo , Neoplasias Hepáticas/genética , Neoplasias Hepáticas/metabolismo , Proliferación Celular , Factor de Crecimiento Derivado de Plaquetas/genética , Factor de Crecimiento Derivado de Plaquetas/metabolismo , Línea Celular Tumoral , Movimiento Celular/genéticaRESUMEN
Volatile organic compounds (VOCs) emitted from vehicle parts and interior materials can seriously affect in-cabin air quality. Prior studies mainly focused on indoor material emissions, while studies of emissions in-cabins were relatively scarce. The emission behaviors of VOCs from vehicle cabin materials can be characterized by three key emission parameters: the initial emittable concentration (C0), diffusion coefficient (Dm), and partition coefficient (K). Based on a C-history method, we have performed a series of tests with a 30 L small-scale chamber to determine these three key emission parameters for six VOCs, benzene, toluene, ethylbenzene, xylene, formaldehyde, and acetaldehyde, from typical vehicle cabin materials, car roof upholstery, carpet, and seat. We found that acetaldehyde had the highest level in the gas-phase concentration and C0, which differs from residential indoor environments where formaldehyde is usually the most prevalent pollutant. The influence of temperature on the key emission parameters was also investigated. When the temperature rose from 25 °C to 65 °C, C0 increased by 40-640%, Dm increased by 40-170%, but K decreased by 38-71% for different material-VOC combinations. We then performed an independent validation to demonstrate the accuracy of the measured key emission parameters. Furthermore, considering that in reality, several materials coexist in vehicle cabins, we made a first attempt at applying a multi-source model to predict VOC emission behaviors in a simulated 3 m3 vehicle cabin, using the key emission parameters obtained from the small-scale chamber tests. The good agreement between the predictions and experiments (R2 = 0.82-0.99) demonstrated that the three key emission parameters measured via chamber tests can be scaled to estimate emission scenarios in realistic vehicle cabin environments. A pollution contribution analysis for the tested materials indicated that the car seat could significantly contribute to the total emissions.