RESUMEN
Citrus melanose, caused by Diaporthe citri, is one of the most important and widespread fungal diseases of citrus. Previous studies demonstrated that the citrus host was able to trigger the defense response to restrict the spread of D. citri. However, the molecular mechanism underlying this defense response has yet to be elucidated. Here, we used RNA-Seq to explore the gene expression pattern at the early (3 days post infection, dpi) and late (14 dpi) infection stages of citrus leaves in response to D. citri infection, and outlined the differences in transcriptional regulation associated with defense responses. The functional enrichment analysis indicated that the plant cell wall biogenesis was significantly induced at the early infection stage, while the callose deposition response was more active at the late infection stage. CYP83B1 genes of the cytochrome P450 family were extensively induced in the callus deposition-mediated defense response. Remarkably, the gene encoding pectin methylesterase showed the highest upregulation and was only found to be differentially expressed at the late infection stage. Genes involved in the synthesis and regulation of phytoalexin coumarin were effectively activated. F6'H1 and S8H, encoding key enzymes in the biosynthesis of coumarins and their derivatives, were more strongly expressed at the late infection stage than at the early infection stage. Collectively, our study profiled the response pattern of citrus leaves against D. citri infection and provided the transcriptional evidence to support the defense mechanism.
Asunto(s)
Ascomicetos , Citrus , Xanthomonas , Hojas de la Planta/genética , Hojas de la Planta/microbiología , Enfermedades de las Plantas/genética , Enfermedades de las Plantas/microbiología , Xanthomonas/fisiologíaRESUMEN
In this study, we produced S, N co-doped CNDs (SN@CNDs) by using dimethyl sulfoxide (DMSO) and formamide (FA) as single sources of S and N, respectively. We varied the S/N ratios by adjusting the volume ratios of DMSO and FA and investigated their effect on the red-shift of the CNDs' absorption peak. Our findings demonstrate that SN@CNDs synthesized using a volume ratio of 5:6 between DMSO and FA exhibit the most significant absorption peak redshift and enhanced near-infrared absorption performance. Based on comparative analysis of the particle size, surface charge, and fluorescence spectrum of the S@CNDs, N@CNDs, and SN@CNDs, we propose a possible mechanism to explain the change of optical properties of CNDs due to S, N doping. Co-doping creates a more uniform and smaller band gap, resulting in a shift of the Fermi level and a change in energy dissipation from radioactive to non-radiative decay. Importantly, the as-prepared SN@CNDs exhibited a photothermal conversion efficiency of 51.36% at 808 nm and demonstrated exceptional photokilling effects against drug-resistant bacteria in both in vitro and in vivo experiments. Our facile method for synthesizing S and N co-doped CNDs can be extended to the preparation of other S and N co-doped nanomaterials, potentially improving their performance.