RESUMEN
Trichomonas vaginalis is responsible for the prevalence of trichomoniasis, which may be one of the most epidemic nonviral sexually transmitted pathogens. Extracellular traps (ET) are a unique form of innate immunity against infection; they bind to and kill microorganisms. However, the effect of T. vaginalis on ET release in the human monocytic cell line THP-1 remains unclear. In the present study, the morphology of ET derived from THP-1 in response to T. vaginalis was observed by scanning electron microscopy (SEM). The results demonstrated ET entangling T. vaginalis. Then, the colocalization of histone (H3) and myeloperoxidase (MPO) with DNA was observed via fluorescence confocal microscopy. Colocalization revealed the classic characteristics of DNA decorated with H3 and MPO. T. vaginalis significantly increased reactive oxygen species (ROS) and THP-1-derived ET. In addition, we measured the levels of lactic dehydrogenase (LDH) and the phosphorylation of the P38 and ERK1/2 MAPK signaling pathways. The results indicated that the formation of ET induced by T. vaginalis was related to phosphorylation of the P38 and ERK1/2 MAPK signaling pathways but not to LDH levels. These data confirmed the phenomenon of THP-1-derived ET being triggered by T. vaginalis in vitro; this process may play a pivotal role in innate immunity during defense against T. vaginalis infection.
Asunto(s)
Trampas Extracelulares/inmunología , Monocitos/inmunología , Tricomoniasis/inmunología , Trichomonas vaginalis/fisiología , Línea Celular , Trampas Extracelulares/parasitología , Humanos , Inmunidad Innata , Sistema de Señalización de MAP Quinasas , Peroxidasa/inmunología , Especies Reactivas de Oxígeno/inmunología , Tricomoniasis/parasitologíaRESUMEN
Chlorogenic acid (CGA), one of the most abundant polyphenols in the diet, has been reported to have potent anti-inflammatory properties. However, the effect of CGA on lipopolysaccharide (LPS)-induced mice mastitis has not been investigated. The purpose of the present study was to elucidate whether CGA could ameliorate the inflammation response in LPS-induced mice mastitis and to clarify the possible mechanism. The mouse model of mastitis was induced by injection of LPS through the duct of mammary gland. CGA was administered intraperitoneally with the dose of 12.5, 25, and 50mg/kg respectively 1h before and 12h after induction of LPS. In this study, the effect of CGA on LPS-induced mice mastitis was assessed through histopathological examination, ELISA assay, and western blot analysis. The results showed that CGA significantly reduced TNF-α, IL-1ß, and IL-6 production compared with LPS group. Besides, western blot analysis showed that CGA could inhibit the expression of TLR4 and the phosphorylation of NF-κB and IκB induced by LPS. These results suggested that anti-inflammatory effects of CGA against LPS-induced mastitis may be due to its ability to inhibit TLR4-mediated NF-κB signaling pathway. Therefore, CGA may be a potent therapeutic reagent for the prevention of the immunopathology encountered during Escherichia coli elicited mastitis.
Asunto(s)
Ácido Clorogénico/uso terapéutico , Lipopolisacáridos/toxicidad , Mastitis/metabolismo , Mastitis/prevención & control , FN-kappa B/fisiología , Receptor Toll-Like 4/fisiología , Animales , Ácido Clorogénico/farmacología , Relación Dosis-Respuesta a Droga , Femenino , Masculino , Mastitis/inducido químicamente , Ratones , Ratones Endogámicos BALB C , FN-kappa B/antagonistas & inhibidores , Transducción de Señal/efectos de los fármacos , Transducción de Señal/fisiología , Receptor Toll-Like 4/antagonistas & inhibidoresRESUMEN
Cepharanthine (CEP), a biscoclaurine alkaloid isolated from Stephania cepharantha Hayata, has been reported to have potent anti-inflammatory properties. However, the anti-inflammatory effects of CEP on a mouse model of lipopolysaccharide (LPS)-induced mastitis and its underlying molecular mechanisms remain to be elucidated. The purpose of the present study was to investigate the effects of CEP on LPS-induced mouse mastitis. The mouse model of mastitis was induced by inoculation of LPS through the canals of the mammary gland. CEP was administered intraperitoneally at 1 h before and 12 h after induction of LPS. The results show that CEP significantly attenuates the infiltration of neutrophils, suppresses myeloperoxidase activity, and reduces the levels of TNF-α, IL-1ß, and IL-6 in LPS-induced mouse mastitis. Furthermore, CEP inhibited the phosphorylation of NF-κB p65 subunit and the degradation of its inhibitor IκBα. All the results suggest that CEP exerts potent anti-inflammatory effects on LPS-induced mouse mastitis. Accordingly, CEP might be a potential therapeutic agent for mastitis.
Asunto(s)
Antiinflamatorios/farmacología , Bencilisoquinolinas/farmacología , Lipopolisacáridos , Glándulas Mamarias Animales/efectos de los fármacos , Mastitis/tratamiento farmacológico , FN-kappa B/metabolismo , Transducción de Señal/efectos de los fármacos , Animales , Modelos Animales de Enfermedad , Relación Dosis-Respuesta a Droga , Regulación hacia Abajo , Femenino , Mediadores de Inflamación/sangre , Interleucina-1beta/sangre , Interleucina-6/sangre , Glándulas Mamarias Animales/inmunología , Glándulas Mamarias Animales/metabolismo , Glándulas Mamarias Animales/patología , Mastitis/sangre , Mastitis/inducido químicamente , Mastitis/inmunología , Mastitis/patología , Ratones , Ratones Endogámicos BALB C , Infiltración Neutrófila/efectos de los fármacos , Peroxidasa/metabolismo , Factor de Necrosis Tumoral alfa/sangreRESUMEN
Aim To study the protective effects of Xinnaoshenkang (XNSK) against focal cerebral injury caused by ischmia-reperfusion in rats and its mechanism. Methods The focal brain ischmia-reperfusion model in rats was made through by using an intraluminal monofilament to occlude the middle cerebral artery for 1.5 h and then reperfusing for 24h.Spectrophotometric assay was used to measure the contents of malondial-dehyde (MDA), lactic acid (LA),superoxide dismutase(SOD), reactive oxygen species(ROS), nitricoxide synthase(NOS) and several ATPase in cerebral cortex homogenates from rats. The effects on platelet aggregation were also observed. Results Compared with model and positive control groups,88,175,350 mg?kg-1 XNSK groups were found having significant inhibition of cerebral infarction,MDA,LA,ROS,NOS,platelet aggregation and significant increase of the activity of SOD,ATPase. Conclusion XNSK has protective effects against focal cerebral injury caused by ischmia-reperfusion in rats.