Detection of the proportion of myeloid progenitor cells and lymphocytes in bone marrow by flow cytometry immunophenotyping can effectively identify aplastic anemia and hypoplastic myelodysplastic syndrome / 中华检验医学杂志

Objective:To investigate the flow cytometric characteristics of bone marrow in patients with aplastic anemia (AA) and hypoplastic myelodysplastic syndrome (hypoMDS) and its value in differential diagnosis between AA and hypoMDS.Methods:A total of 618 patients were recruited from Shandong Provincial Hospital affiliated to Shandong First Medical University from January 2017 to December 2021, including 441 patients as controls with abnormal peripheral blood counts but confirmed to have no AA, hypoproliferative MDS, or other hematological tumors, 57 patients with AA, 52 patients with hypoMDS and 68 patients with diluted bone marrow. The proportions of bone marrow myeloid progenitor cell, lymphocyte, neutrophil and erythroid cell in bone marrow were analyzed by flow cytometry immunophenotyping. The differences between the four groups were compared by Kruskal-Wallis one-way ANOVA. The diagnostic efficacy was evaluated by the receiver operating characteristic (ROC) curve.Results:The proportion of myeloid progenitor cells in control group was [0.30%(0.20%, 0.46%)]; the proportion of myeloid progenitor cells in AA group was [0.00 (0.00, 0.04)]%, while the proportion of myeloid progenitor cells in hypoMDS group was [3.10%(1.25%, 6.71%)]. The proportion of myeloid progenitor cells in AA group was lower than that in control group( χ2=302.90, P<0.001), while the proportion of myeloid progenitor cells in the bone marrow of patients with hypoMDS was higher than that in the control group ( χ2=203.88, P<0.001). The area under the ROC curve for identifying AA and hypoMDS by the ratio of myeloid progenitor cells in bone marrow is 0.996, with the sensitivity of 96.2% and the specificity of 100% when the cutoff value is 0.21%. The proportion of bone marrow lymphocytes in control group was [10.39%(7.33%, 14.80%)]; the proportion of bone marrow lymphocytes in AA group was [52.67%(42.20%, 67.68%)], and the proportion of bone marrow lymphocytes in hypoMDS group was [24.68%(15.51%, 35.19%)]. The proportion of bone marrow lymphocytes in AA group or hypoMDS group was higher than that in control group( χ2=298.74, P<0.001; χ2=194.33; P<0.001), and the proportion of bone marrow lymphocytes in AA group is higher than that in hypoMDS group, with a statistically significant difference( χ2=104.41, P=0.002). The area under the ROC curve for identifying AA and hypoMDS by the ratio of bone marrow lymphocyte is 0.882, with the sensitivity of 78.9% and the specificity of 92.3% when the cutoff value is 41.6%. Conculsion:Patients with AA and hypoMDS could be differentiated effectively by the proportions of myeloid progenitor cell and lymphocyte in bone marrow by flow cytometry immunophenotyping.

Menopausal hormone therapy on breast lesions in perimenopausal women / 预防医学

Objective@#To explore the correlation between menopausal hormone therapy（MHT）and breast lesions in perimenopausal women,and to provide evidence for safe use of MHT. @*Methods@#The 40-60 year-old women who visited Hangzhou Women's Hospital and met the diagnostic criteria for perimenopausal syndrome were recruited. The intervention group received MHT and was divided into three subgroups according to the MHT regimen：estrogen-progesterone cycle therapy（A）,estrogen-progesterone continuous therapy（B）,estrogen therapy（C）. The control group did not receive MHT. All the patients received regular mammography to quantify and evaluate breast lesions. The generalized estimating equation was used to analyze the changes of breast lesions between different groups.@*Results@#There were 80 cases in the intervention group,with 49 in group A,26 in group B,5 in group C,and 80 cases in the control group. After two years of follow-up,there was no statistically significant differences of time,group and interaction in breast density,volume of breast fibrous tissue and the volume of breast between three intervention groups and the control group（P>0.05）; there was no statistically significant differences of group and interaction in positive rate of calcification and breast mass between the intervention group and the control group（P>0.05）. @*Conclusion@#Receiving MHT intervention for two years did not increase the risk of breast lesions.

Molecular mechanism and functional role of macrophage colony­stimulating factor in follicular granulosa cells.

Our previous demonstrated that macrophage colony­stimulating factor (M­CSF) stimulated the production of estradiol (E2) and progesterone (P) in luteinized granulosa cells (GCs), and that its secretion may be regulated by follicle­stimulating hormone (FSH). The present study aimed to examine the effect of M­CSF alone or with Letrozole treatment on the function of non­luteinizing granulosa cells, using the COV434 cell line, and its interaction with FSH. Human luteinized granulosa cells (LGC) were isolated from the follicular fluid of superovulated infertile patients (average age, 30.8±2.1 years) undergoing an intracytoplasmic sperm injection. The LGC were cultured with various concentrations of recombinant human macrophage colony stimulating factor (rhM­CSF; 0, 10, 25, 50 or 100 ng/ml), rhM­CSF+Letrozole (10­6 mol/l), rhFSH (0, 10, 25, 50 or 100 IU/ml), or rhFSH+Letrozole (10­6 mol/l). E2 concentrations in the media were measured using ELISA. The expression levels of the FSH receptor and the M­CSF receptor were detected via reverse transcription­quantitative polymerase chain reaction. Following COV434 cell treatment with M­CSF, cell proliferation was quantified using the MTS assay and protein expression was detected by western blotting. It was demonstrated that M­CSF and FSH stimulated the production of E2. The production of FSH receptors was enhanced by rhM­CSF or rhM­CSF+Letrozole in vitro in a dose­dependent manner. It was observed that rhFSH promoted the expression of the M­CSF receptor, at a certain concentration. Proliferation of COV434 cells increased in a dose­dependent manner following treatment with RhM­CSF. Furthermore, M­CSF induced the phosphorylation of c­Jun N­terminal kinase (JNK) and p38; however, the level of E2 in the medium was not altered when the cells were pretreated with the JNK inhibitor SP600125 or the p38 inhibitor SB203580. The present study suggested that M­CSF may be important in regulating the response of GCs to gonadotropin and may have a promotive effect in the early phase of follicular development. The biological effects of M­CSF may partially be attributed to activation of the JNK and p38 signaling pathways. M­CSF may represent a novel follicular development regulator agent in the future.

Acute myeloid leukemia with t(16;21)(p11;q22):two cases report and literatures review / 白血病·淋巴瘤

Objective To investigate the clinical and laboratory characteristics of acute myeloid leukemia (AML) with t (16;21)(p11;q22) translocation.Methods Two patients diagnosed by morphology,cytochemical stain,immunology,cytogenetics and genetic testing.Similarities and differences of clinical characteristics and laboratory examination were analysed,along with a review of the literatures.Results According to the FAB classification,one patient was M4 and the other one was M1.The cytogenetic aberrations were 46,XY,t(16;21)(p11;q22)[16]/47,XY,t(16;21)(p11;q22),+21[4] of ease 1 and 46,XX,t(16;21)(p11;q22)[20] of case 2.Cytophagocytosis and CD56 antigen expression were found in both cases.The prognosis was poor in both cases.Conclusions AML with t(16;21)(p11;q22) is a specific type,which has unusual characteristics of morphology,immunology,cytogenetics,clinical feature.The prognosis of the patients is poor,so stem-cell transplantation maybe the only and the first choice of treatment.

Protective effects of da chai hu granules (DCHKL) against alloxan (AXN)-induced rat pancreatic islets damage / 药学学报

The protective effects of Da Chai Hu Granules (DCHKL) on islet cells which were incubated with 4 mmol x L(-1) alloxan (AXN) were studied. The viability of islet cells were measured with MTT. Insulin released into medium and in islets was detected by radioimmunoassay. Cell apoptosis rate was determined by flow cytometry. The expression of anti-apoptotic gene Bcl-2 and pro-apoptotic gene Bax in islet cells were measured with RT-PCR (reverse transcription polymerase chain reaction). Serum containing DCHKL can promote the activity of islet cells significantly (P < 0.01). Basal insulin secretion and high glucose-stimulated insulin secretion increased significantly (P < 0.01). Serum containing DCHKL can inhibit apoptosis of islet cells, the ratio of apoptosis was decreased. Serum containing DCHKL increased expression of Bcl-2 mRNA and decreased expression of Bax mRNA. DCHKL can significantly promote proliferation of islet cells and increase the amount of basal secretion of pancreatic islet cells and high glucose-stimulated insulin secretion. The expression of Bcl-2 increased significantly. The expression of Bax decreased significantly. DCHKL have a protective effect on the islet cells.

A clinical and laboratory investigation of myelodysplastic/myeloproliferative neoplasms / 中华检验医学杂志

ObjectiveTo investigate distingwished clinical and experimental characteristics of the four main subtypes in myelodysplastic/myeloproliferative neoplasms (MDS/MPNs).MethodsMDS/MPNs 53 cases from Provincial Hospital Affiliated to Shandong University,including 24 cases CMML,13 cases aCML,12 cases JMML,4 cases MDS/MPN-U,were analyzed regarding to 2001 WHO classification.Morphology (M) of peripheral and bone marrow blood cells were observed under microscope.FCM was used in immunological(Ⅰ) analyse on blasts and myelomonocytes in peripheral blood and bone marrow.G-banding technique was used in cytogenetic (C)examination.PCR was used in molecular genetic (M) mutation detection.Numeric data,such as mean Hb,WBC,PLT et al,among several groups,were compared using Single-factor analysisof variance.Student-Newman-Keulstestwasuseincomparingmeansof two groups.Proportions,such as percentage of clinical features,immunological and cytogenetic abnormal cases among different groups,were compared using Chi-square test or Fisher exact test.Results( 1 ) In the course of MDS/MPNs,there were 46 cases (86.8％ ) had paleness and fatigue 33 cases (62.5％ ) had palpable spleen.JMML had most fever and enlargement of lymph node (75.0％,75.0％ ),statistically distinguished from CMML ( 12.5％,12.5％ ) (x2 =14.89,17.98,P ＜ 0.05 ).(2) The hemoglobin was ( 83.1 ± 24.6 ) g/L.WBC counts were ( 19.8 ± 8.1 ) × 109/L.PLT counts were ( 158.7 ± 108.2) x 109/L.Immature neutrophils and blasts were found in peripheral blood.(3)JMML and CMML had most monocytes absolute counts among the subtypes (4.25 ±0.76) (3.62 ±0.76).(4) Almost 100％ JMML had monocytes abnormalities.(5)For 15 cases were detected immunological characteres by FCM,13 cases showed abnormalities.(6)For 29 cases of MDS/MPNs had been analyzed chromosome karyotypes and 12 out of them (41.4％) were abnormal,Ph chromosomes and those AML-defining translocations were all negative,+ 8 and 7-involved- karyotypes were more frequent.(7)23 cases were detected molecular genetic features,in which were all negative.BCR/ABL1 and JAK2 V617F mutation were all negative in the 13 cases of aCML.JAK2 V617F mutation was positive in 1 case of MDS/MPN-U.ConclusionsMost MDS/MPNs had paleness and fatigue,light to mild anemia,cytosis,monocytes low grade of blast and immature neutrophils in peripheral blood with dysplasia in bone marrow.JMML seems has more severe clinical features and more distinguishing laboratory characters.Immunological abnormalities and abnormalkaryotypes are found frequently in MDS/MPNs with no statistical differences among the four subtypes.There is no specific molecular abnormals in MDS/MPNs.( Chin J Lab Med,2012,35:832-837)

Effect of jinqiaomai on expression of TLR2/4, MyD88 mRNA and IkappaB-alpha in lung tissue of rats with Klebsiella pneumonia / 中国中药杂志

<p><b>OBJECTIVE</b>To study the mechanism of protective effect of Jinqiaomai (JQM) on lung injury induced by Klebsiella pneumonia in rats.</p><p><b>METHOD</b>The model of rats with Klebsiella pneumonia was established. The male SD rats were randomly divided into control group, model group, JQM (6, 3, 1.5 g x kg(-1)) three groups, levofloxacin (25 mg x kg(-1)) group, JQM (3 g x kg(-1)) + levofloxacin (25 mg x kg(-1)) group. The contents of IL-1beta, ICAM-1 and INF-gamma in the lung tissue homogenate were measured by radio-immunoassay and Elisa. TLR2/4 mRNA and MyD88 mRNA expression were detected by RT-PCR. IkappaB-alpha expression was detected by Western Blot.</p><p><b>RESULT</b>The rats of model group had obvious lung injury, but those of JQM, JQM + levofloxacin and levofloxacin groups had less injury. The contents of IL-1beta, ICAM-1 and INF-gamma in lung tissue homogenate and the expressions of TLR2/4 mRNA, MyD88 mRNA and IkappaB-alpha in lung of model group were significantly higher than those in the control group (P < 0.05 or P < 0.01), while IL-1beta, ICAM-1 and INF-gamma of JQM groups were significantly lower than those of model group (P < 0.05 or P < 0.01). The expressions of TLR2/4 mRNA, MyD88 mRNA and IkappaB-alpha of JQM (6.3 g x kg(-1)) groups were significantly lower than those of model group(P <0. 05 or P <0. 01).</p><p><b>CONCLUSION</b>The lung injury induced by Klebsiella pneumonia is related to TLR2/4, MyD88 mRNA and IkappaB-alpha. To decrease the excessive expression of TLR2/4, MyD88 mRNA and IkappaB-alpha might be the main mechanism of protective effect of Jinqiaomai on lung injury.</p>

Effect of nerve growth factor in promoting survival of super-length random flap / 中国综合临床

Objective To study the effect of nerve growth factor(NGF)in survival of super-length random flap.Methods The experiment employed the animal model of random flap in Wistar's rat back,applied 66.7 μg NGF locally in trial group's flap at the first day after operation with auto-control.The survival proportion,skin temperature and survival rate of random flap were observed,and the blood flow,the blood flow velocity,the blood flow volume change of random flap were measured by the laser and photic-conduct fiber means in differ time.Results The survival proportion(7.12±1.54)cm2 and survival rate(92±5)%of random flap of trial group was obviously higher(P＜0.05)than those in control group[(5.23±0.19)cm2,(69±5)%]after pedicle division.The blood flow,the blood flow velocity,the blood flow volume and skin temperature in the midge and at the end of random flap in the trial group were obviously higher than those in control group 1 day after operation(P＜0.05),and the blood flow velocity in the pedicle of random flap in the trial group was increased markedly(P＜0.05).The blood flow volume and skin temperature in the pedicle of random flap in the trial group were higher than those in control group 3 days after operation(P＜0.05).The survival of randomflapinthetrial groupwere very significantly increased than those in control group 7 days after operation(P＜0.01).Conclusion NGF can increase flap's blood flow,blood flow velocity and blood flow volume,enhancing survival of the big-proportioned and super-length random flap.

Clinical effect and safety of miconazole nitrate 1200 mg in treating vulvovaginal candidiasis / 中华妇产科杂志

Objective To investigate the clinical effect and safety of miconazole nitrate 1200 mg in treating vulvovaginal candidiasis(VVC).Methods An open,multicentre,non case control clinical trial was conducted in 568 patients suffering from VVC from Jul 1,2006 to Nov 30,2006.Routine examination,score of clinical symptoms and physical signs,mycetology test and safety evaluation were done in all patients before treatment,7-14 days after treatment and 30 days after treatment.Results Seven to fourteen days after treatment,563 patients could be followed and 323 patients(57.30k)were cured.The overall effective rate was 90.2%.The mycologic cure rate was 91.3%(514).Thirty days after treatment,480 patients could be followed and 411 patients(85.6%)were cured.The total effective rate was 96.0%.Mycologic cure rate was 92.3%(443/480).Adverse effect rate was 2.7%(15/563)and they were relieved without any treatment in one or two days.Conclusions Miconazole nitrate 1200 mg is effective in the treatment of WC,with good compliance and few adverse effects.Moreover,it can be accepted easily.

Clinical characteristics and treatment of acute asthma exacerbations during pregnancy / 中华妇产科杂志

Objective To study the clinical characteristics of acute asthma during pregnancy,focusing on management of pregnant women presenting with an acute exacerbation.Methods A prospective observational study was conducted in 30 cases of acute exacerbations during pregnancy.Results The prognosis of 25 patients with mild or moderate asthma was good for both mother and fetus;none had severe complications.Among 5 women who had severe exacerbations,there were 4 pulmonary co-infections,1 with respiratory acidosis,1 with respiratory acidosis plus metabolic allkalosis,and 4 with fetal growth-restriction.Conclusions,This study highlights that pregnancy can worsen asthma control in some women.Effective management and prevention of asthma exacerbations during pregnancy is key to ensuring the safety of the mother and the fetus.

Expression of macrophage colony-stimulating factor in human luteinized granulosa cells / 中华妇产科杂志

Objective To detect and localize the expression of macrophage colony stimulating factor (M CSF) in human luteinized granulosa cells and investigate the effects of follicle stimulating hormone (FSH) on M CSF production by human luteinized granulosa cells in vitro Methods Human luteinized granulosa cells were isolated from follicular fluid of superovulated infertile patients undergoing intracytoplasmatic sperm injection Some of the luteinized granulosa cells were used for detecting M CSF by immunocytochemical staining (ABC method) Most of them were cultured with HAM′s F 10 medium alone or plus various concentrations of FSH (2, 5, 15, 25 IU/ml) The media were collected after 72 hours and their M CSF contents were measuered by a solid phase enzyme linked immunosorbant assay Results About 80% of the luteinized granulosa cells were positively stained for M CSF antibody The immunoreactive signals of M CSF were specially located in the cytoplasma of the luteinized granulosa cells No immunoreactivity of M CSF was observed in vaginal squamous epithelial cells through contaminations during transvaginal oocyte retrieval The concentration of M CSF in cultured luteinized granulosa cells medium without FSH stimulation was low However, addition of 2, 5, 15, 25 IU/ml concentrations of FSH caused significant dose dependent increases of M CSF production after 72 hours culture by luteinized granulosa cells ( P

Functional imaging of Parkinson disease after treated by deep brain stimulation of subthalamic nucleus / 第二军医大学学报

Objective:To investigate the metabolism changes in corpus striatum of hemiparkinsonian monkeys after chronic high frequency stimulation in subthalamic nucleus (STN) by SPECT and PET imaging. Methods: Two hemiparkinsonian monkeys, induced by unilateral internal carotid artery infusion of 1-methyl-4-phenyl-1,2,3,6-tetrahydropy-rindine (MPTP), were implanted with stimulation electrodes in STN of lesioned side according to stereotaxic atlas of the monkey brain. SPECT and PET examination were performed before and 1, 3 months after stimulation. Results: PD symptoms of monkeys, including rigidity, bradykinesia and gait abnormality, were significantly improved. SPECT imaging indicated that dopamine transporter (DAT) binding to corpus striatum in stimulated side increased and D 2 receptor (D 2 R) decreased to the level of unlesioned side after stimulating. PET imaging showed that right corpus striatum had lower density than left corpus striatum before stimulation. One month after chronic high frequency stimulation, bilateral corpus striatum had low density, but the right was slightly higher than that of the left;3 months after stimulation the right was obviously higher than the left . Conclusion: The symptoms of hemiparkinsonian monkeys can be obviously improved by STN deep brain stimulation(DBS). The increased DAT binding and glucose metabolism of corpus striatum in stimulated side and the decreased D 2 R binding after stimulation suggest that the activity of striatal dopaminergic system may be promoted by STN DBS.