Assessment of therapeutic effects of low-energy laser for the treatment of burning mouth syndrome in elderly patients / 中华老年医学杂志

Objective:To assess the clinical efficacy of low-level laser therapy(LLLT)for the treatment of burning mouth syndrome(BMS)in elderly patients.Methods:As a randomized controlled study, 60 elderly BMS patients treated in the department of stomatology of Beijing Hospital from September 2018 to August 2021 were randomly divided into a laser group and a control group, including 7 man and 53 women, with an average age of(68.27±6.38)years.Patients in the laser group were treated with LLLT, and patients in the control group were treated with non-energy red light and oryzanol.The visual analogue scale and self-rating anxiety and depression scale were used to evaluate the two groups of patients.Results:After LLLT, the effectiveness rate of the laser group was 86.67%(26/30)on the 28th and 90th day, which was significantly higher than 63.33%(19/30)of the control group( χ2=4.356, P=0.037). When data for pre-treatment, the 28th day after treatment and the 90th day after treatment for the laser group were compared, results showed that after LLLT, pain symptoms of BMS patients were relieved and it lasted for some time, and anxiety and depression also improved, but there were episodes of relapse as time went on.Visual analogue scale and self-rating anxiety scores and depression scale scores of BMS patients for pre-treatment, the 28th and 90th days after treatment in the two groups were compared.Only self-rating anxiety scale scores of the laser group was lower than those of the control group on the 28th day, with statistical significance( t=2.622, P=0.011), indicating that LLLT could alleviate anxiety for BMS patients in the short term, but had no significant effect on patient depression.However, in the long term, LLLT had no significant effect on relieving anxiety and depression. Conclusions:LLLT has a certain effect on improving pain symptoms and anxiety in elderly BMS patients.

Successful treatment of oral human papilloma by local injection 5-aminolevulinic acid-mediated photodynamic therapy: A case report.

Oral human papilloma (HPV) virus is very common in patients. Deep location of lesions can lead to poor therapeutic effects. We are reporting a case of 52-year-old male who was diagnosed of oral papilloma on the left side of the palatoglossal arch. He was treated with local injection of 5-aminolevulinic acid-mediated photodynamic therapy for 2 sessions. There was no obvious recurrence at 6 months of follow-up. This case suggests that sodium chloride injection diluted ALA photosensitizer and injection at lesion basement can achieve good therapeutic results.

Study on the oral mucosal diseases in patients with cerebrovascular diseases / 中华流行病学杂志

Objective@#To investigate the prevalence of oral mucosal diseases (OMD) in patients with cerebrovascular disease.@*Methods@#A total of 182 patients with cerebrovascular disease and 166 controls were examined for OMD to compare the differences of prevalence rates.@*Results@#The prevalence of OMD in patients with cerebrovascular disease appeared higher than that in the control group. Oral candidiasis was most commonly seen (11.1%, 20/182), followed by fissured tongue (5.0%, 9/182), traumatic ulcer (2.8%, 5/182), herpes labialis (2.2%, 4/182), recurrent oral ulcer (1.6%, 3/182), chronic cheilitis (1.6%, 3/182) and oral leukokeratosis (1.6%, 3/182).@*Conclusion@#Patients with cerebrovascular diseases were susceptible to OMDs, especially to oral candidiasis that called for more attention.

Observation of clinical effect of photodynamic therapy on oral leukoplakia in elderly patients / 中华老年医学杂志

Objective@#To observe the clinical efficacy of photodynamic therapy on oral leukoplakia in elderly patients.@*Methods@#In this prospective study, fifteen elderly patients with oral leukoplakia were treated with 5-aminolevulinic acid-based photodynamic therapy(ALA-PDT). After 2 hours of dressing, patients were irradiated using a 635nm semiconductor laser with the power density of 300mW/cm2.Each lesion was irradiated for 6 minutes with energy density of 100J/cm2.The curative effect was evaluated and the adverse reactions were recorded based on the observation of lesion changes.@*Results@#Of the 15 lesions in 15 patients, 4 lesions were treated once, 3 lesions were treated twice.And 6 lesions were cured after three treatments, and 2 patients were cured after four treatments.The average treatment frequency was(2.4±1.1)treatment.The postoperative adverse reaction was pain.The average visual analogue scale was(2.2±0.8)scores after operation, and the duration of pain was(2.6±1.1)days after operation.There was no recurrence for 3 to 9 months after operation.No abnormalities of oral sensation and function were found in patients.@*Conclusions@#ALA-PDT is an effective method for the treatment of oral leukoplakia in elderly patients.

Observation of clinical effect of photodynamic therapy on oral leukoplakia in elderly patients / 中华老年医学杂志

Objective To observe the clinical efficacy of photodynamic therapy on oral leukoplakia in elderly patients.Methods In this prospective study,fifteen elderly patients with oral leukoplakia were treated with 5 aminolevulinic acid-based photodynamic therapy(ALA PDT).After 2 hours of dressing,patients were irradiated using a 635nm semiconductor laser with the power density of 300mW/cm2.Each lesion was irradiated for 6 minutes with energy density of 100J/cm2.The curative effect was evaluated and the adverse reactions were recorded based on the observation of lesion changes.Results Of the 15 lesions in 15 patients,4 lesions were treated once,3 lesions were treated twice.And 6 lesions were cured after three treatments,and 2 patients were cured after four treatments.The average treatment frequency was (2.4 ± 1.1) treatment.The postoperative adverse reaction was pain.The average visual analogue scale was (2.2 ± 0.8) scores after operation,and the duration of pain was(2.6±1.1)days after operation.There was no recurrence for 3 to 9 months after operation.No abnormalities of oral sensation and function were found in patients.Conclusions ALA-PDT is an effective method for the treatment of oral leukoplakia in elderly patients.

Impact of tooth structure and fatigue cycles on the fracture strength of glass-ceramic onlays in the elderly / 中华老年医学杂志

Objective To investigate the impact of tooth structure and fatigue cycles on the fracture strength of glass-ceramic onlays for molar restoration in the elderly.Methods A total of 72 extracted molars due to periodontitis from people aged 60 years or over were selected and flattened to different levels of the occlusal surface.The percentage of enamel was calculated.Two groups with different percentages of enamel were selected from 72 molars,one group with(80±10)％ enamel or the E group and the other group with (20±10)％ enamel or the D group,with 12 samples in each group.IPS e.max CAD discs(diameter=9 mm,thickness=1.3 mm)were cemented to the prepared sample surface by using Variolink N resin cement system.Furthermore,three subgroups were formed randomly from each group(n=4,in each group).Subgroups were treated with 5,100 000 and 1000 000 fatigue cycles,respectively,with the same load(180 N,1.5 Hz)in water,following thermal cycling(5～55 ℃ for 1 500 cycles),and then fracture strength was tested.Results The fracture strength was(2 761.8±477.2)N in the E group and(1 100.1±216.9)N in the D group.The two-factor analysis of variance indicated that tooth structure had a significant influence on fracture strength(F =98.51,P＜0.05),while the influence of fatigue cycles on fracture strength was not significant (F =0.22,P ＞0.05).Enamel percentage had a positive linear correlation with fracture strength(r=0.915,=10.66,P ＜0.05).Conclusions Enamel percentage has a positive correlation with fracture strength of glass-ceramic onlays.For elderly people who have severe tooth wear,preserving more enamel within the adhesive area can increase the fracture strength of ceramic onlays for an extended period of time.

A meta-analysis of the effect of periodontal instrumentation in combination with triple therapy on the eradication of gastric Helicobacter pylori / 中华老年医学杂志

Objective To systematically assess the clearance rates of periodontal instrumentation in combination with triple therapy and triple therapy alone for patients with gastric Helicobacter pylori(HP) infection.Methods PubMed,EMbase,Medline,CNKI,SinoMed and Wan Fang databases were searched to collect controlled clinical trials,conducted between March 1997 and March 2017,on periodontal therapy combined with triple therapy or triple therapy alone for patients with gastric HP infection.Two reviewers independently screened the literature,extracted data,and assessed the risk of bias of included studies.Then meta-analysis was performed using RevMan 5.3 software.Results Eight studies involving 777 patients were included.The results of the metaanalysis showed that clearance rates of HP were 52.1％ for triple therapy alone and 81.1％ for periodontal therapy combined with triple therapy.The difference was statisticatly significant (OR =4.08,95％CI:2.94～ 5.67,P＜0.001).Conclusions Currently available evidence suggests that,compared with triple therapy alone,periodontal therapy in combination with triple therapy produces higher rates of gastric HP eradication.

A meta-analysis of the effect of periodontal instrumentation in combination with triple therapy on the eradication of gastric Helicobacter pylori / 中华老年医学杂志

Objective To systematically assess the clearance rates of periodontal instrumentation in combination with triple therapy and triple therapy alone for patients with gastric Helicobacter pylori(HP) infection.Methods PubMed,EMbase,Medline,CNKI,SinoMed and Wan Fang databases were searched to collect controlled clinical trials,conducted between March 1997 and March 2017,on periodontal therapy combined with triple therapy or triple therapy alone for patients with gastric HP infection.Two reviewers independently screened the literature,extracted data,and assessed the risk of bias of included studies.Then meta-analysis was performed using RevMan 5.3 software.Results Eight studies involving 777 patients were included.The results of the metaanalysis showed that clearance rates of HP were 52.1％ for triple therapy alone and 81.1％ for periodontal therapy combined with triple therapy.The difference was statisticatly significant (OR =4.08,95％CI:2.94～ 5.67,P＜0.001).Conclusions Currently available evidence suggests that,compared with triple therapy alone,periodontal therapy in combination with triple therapy produces higher rates of gastric HP eradication.

Clinical performance of fiber post restorations in elderly patients with residual root and crown / 中华老年医学杂志

Objective To investigate the clinical performance of fiber post restorations in elderly patients with residual root and crown and to analyze the factors affecting the clinical success rate.Methods A total of 66 elderly patients requiring post restoration were selected.They were randomly divided into two groups:the observation group (n=33,restored with fiber post-resin cores) and the control group (n=33,restored with cast metal post-cores).All patients were rechecked 1 year and 2years after the restoration.The clinical efficacy and the failure types were recorded.The differences in clinical performance between the 2 groups were compared by using Fisher's exact test.Results The differences in the gingival index and periodontal index in the 2 groups between the experimental teeth and the control teeth were zero.There were no significant differences in dental morphology,root canal treatment failure,tooth color matching and marginal coloration between the 2 groups (all P＞0.05).1 case with post debonding and 1 case with fiber post fracture were found in the observation group and 1 case with marginal coloration was found in control group.There was no significant difference in clinical success rate between the 2 groups (93.94％ vs.96.97％,P＞0.05).Conclusions The clinical success rate in fiber post-resin core restoration is the same as in cast metal post-cores restoration in elderly patients.

Titanium versus polymethyl methacrylate resin complete denture base A phonology evaluation / 中国组织工程研究

BACKGROUND: Several studies have demonstrated that the range,thickness and morphology of oral prosthesis directly influence patient's phonetic function,and titanium base and polymethyl methacrylate(PMMA)resin base yield different influences on phonetic function due to different thicknesses of materials.OBJECTIVE: To analyze the phonetic parameters in one complete denture patient who wear titanium base and PMMA resin base simultaneously and investigate the influences of maxillary complete denture base on phonetic function.METHODS: Through the use of computer-based voice analysis software PRAAT4.4.34,frequency value of the first concentrated frequency area when speaking consonant sounds/x,sh,r,zh,ch,j,q/and voice onset time when speaking consonant sounds/g,k,zh,ch,j,q/were statistically analyzed in the same patient who separately wear titanium complete denture base and PPMA resin complete denture base,and spectrogram of velar stops between complete denture bases was compared.RESULTS AND CONCLUSION: MMPA resin complete denture base was to some extent poor in promoting the recovery of phonetic function owing to great base thickness.Titanium complete denture base with less thickness in the tongue or palate region would help recovery of phonetic function.Extraction and analysis of phonological parameters,such as frequency value of first concentrated frequency area and voice onset time,using CSL computer*based voice analysis system,would provide objective evidence for lamprophony analysis after wearing complete denture.Maxillary denture with thinner base in tongue and palate regions better promotes the recovery of patient's phonetic function.

Cloning, Expression and Immunization of The Hypoxanthine-guanine Phosphoribosyltransferase for Schistosoma japonicum Chinese Strain / 生物化学与生物物理进展

A 1 270 bp full-length cDNA fragment was obtained from the Schistosoma japonicum (Chinese strain) adult cDNA library after the '3' and 5' ends of the incomplete expression sequence tag (EST) of hypoxanthine-guanine phosphoribosyltransferase of Schistosoma japonicum (SjHGPRT) were amplified by the anchored PCR with 2 pairs of primer that were designed according to the published incomplete SjHGPRT EST and the sequence of multiclone sites of library λgt1 1 vector. Sequence analysis indicated that this fragment, with an identity of 82% to hypoxanthine-guanine phosphoribosyltransferase ofSchistosoma mansoni (SmHGPRT), contained a complete open reading frame(ORF). The deduced amino acid sequence showed 83% identity to that of SmHGPRT. This fragment was cloned into the prokaryotic expression vector pQE30, and subsequently sequenced and expressed in Escherichia coli. SDS-PAGE revealed that M of the recombinant protein was about 28 ku. Western-blot analysis showed that the recombinant protein was recognized by the polyclonal antisera from rabbits immunized with Schistosoma japonicum adult worm antigen. Mice vaccinated with recombinant protein revealed significant worm burden, liver eggs per gram (LEPG), fecal eggs per gram (FEPG) and intrauterine eggs of the female worms reduction percentage, compared with the controls. Taken together, the SjHGPRT full-length cDNA can be cloned and expressed in E. coli as a recombinant protein that elicited immunity against the challenge infection with Schistosoma japonicum, indicating its potential as a partia1 protection vaccine candidate.

Influence of post-core on the strength of endodontically treated and crowned teeth / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To investigate the influence of post-core structure on the strength of endodontically treated and crowned teeth with or without a 2.0 mm dentine ferrule.</p><p><b>METHODS</b>A total of 60 recently extracted human maxillary central incisors were endodontically treated and randomly divided into five groups of 12. They were given following treatments: Group A, endodontically treated; Group B, endodontically treated and crowned (PFM); Group C, cast metal post-core with 2.0 mm dentine ferrule and crowned (PFM); Group D, cast metal post-core with no dentine ferrule and crowned (PFM); Group E, prefabricated post and composite core with 2.0 mm dentine ferrule and crowned (PFM). All specimens were stored at 100% humidity at room temperature for 30 days before testing. Each specimen was in a special jig on the MTS 810 universal material testing machine and subjected to a load at a 135-degree angle to the long axis until failure, with crosshead speed of 0.02 cm/minute. Analysis of variance followed by the Newman-Keuls pairwise multiple comparison tests was used to compare the results.</p><p><b>RESULTS</b>There was a statistically significant difference between different restorative methods. The cast metal post-core with 2.0 mm dentine ferrule and crowned teeth had the highest fracture strength (1793.59 +/- 387.93N), followed by endodontically treated intact teeth (1466.68 +/- 240.11N). No significant difference in the fracture strength was found among the other three groups (958.49 +/- 286.02N; 992.98 +/- 291.00N; 994.94 +/- 285.04 N). There was a statistically significant difference in the fracture resistance between crowned teeth with and without 2.0 mm dentine ferrule (P < 0.01).</p><p><b>CONCLUSIONS</b>Not all post-core structure could improve the strength of endodontically treated teeth. The dentine ferrule can effectively improve the fracture resistance of endodontically treated and crowned teeth.</p>

Fracture strength of custom-fabricated celay all-ceramic post and core / 华西口腔医学杂志

<p><b>OBJECTIVE</b>The purpose of this study is to compare the fracture strengths of custom-fabricated Celay all-ceramic post-core, custom cast metal post-core, and prefabricated stainless steel post (Parapost) plus composite resin core with or without a 2.0 mm dentine ferrule.</p><p><b>METHODS</b>A total of 60 recently extracted human maxillary central incisors were endodontically treated and divided into five groups of 12. They were given the following treatments: Group A: Celay ceramic post-core with 2.0 mm dentine ferrule, Group B: Celay ceramic post-core with no dentine ferrule, Group C: cast metal post-core with 2.0 mm dentine ferrule, Group D: cast metal post-core with no dentine ferrule, and Group E: prefabricated post and composite core with 2.0 mm dentine ferrule. All specimens were stored at 100% humidity at room temperature for 30 days before testing. Each specimen was in a special jig at a 45 degrees angle to the long axis and subjected to a load on MTS 810 universal material testing machine until failure, with crosshead speed of 0.02 cm/min. Analysis of variance followed by the Newman-Keuls pairwise multiple comparison test was used to compare the results of the groups tested.</p><p><b>RESULTS</b>There was a statistically significant difference between five groups (P < 0.01). Celay ceramic post-core with 2.0 mm dentine ferrule (758.35 N +/- 119.26 N) and cast metal post-core with 2.0 mm dentine ferrule (756.63 N +/- 166.22 N) had a significantly larger mean failure threshold for fracture than the other three groups which had no significant difference between each other. There was a statistically significant difference between the fracture resistance of Celay post-core restored teeth with and without 2.0 mm dentine ferrule.</p><p><b>CONCLUSION</b>The custom-fabricated Celay post-core could be a choice for clinical use in endodontically treated tooth when the final restoration is an all-ceramic crown and the preparation has a 2.0 mm dentine ferrule.</p>