[Implications of serum matrix metalloproteinase-8 elevation in patients with acute coronary syndrome].

OBJECTIVE: To study the clinical significance of serum matrix metalloproteinase-8 (MMP-8) concentration variation in patients with acute coronary syndrome (ACS). METHODS: Enzyme-linked immunosorbent assay (ELISA) was employed to detect serum MMP-8 content in 80 ACS patients, including 43 with acute myocardial infarction (AMI), and 37 with unstable angina pectoris (UAP), as well as in 43 with stable angina pectoris (SAP) cases and 37 control subjects. The risk factors for atherosclerosis pertaining to age, sex, hypertension, body mass index, smoking, family history, diabetes, hyperlipidemia, etc. were evaluated statistically in each case. RESULTS: Serum MMP-8 concentration was significantly higher in ACS group than in SAP and control groups (Plt;0.01). In ASC group, serum MMP-8 was much higher in AMI patients than in those with UAP (Plt;0.01), but similar between UAP and SAP groups (P>0.05). Logistic regression analysis identified serum MMP-8 concentration as the possible indicator of ACS (B=4.493, P=0.000), and particularly AMI (B=9.961, P=0.000). Linear correlation and linear regression analysis revealed that only neutrophils were associated with serum MMP-8 concentration variation (r=0.274, P=0.001). CONCLUSIONS: Serum MMP-8 concentration is closely associated with ACS, particularly AMI, and may serve as an indicator for predicting ACS and AMI. Neutrophils may be connected with the variation of serum MMP-8 concentration, but not as the major factor.

[Measurement peripheral blood index related to inflammation and ox-LDL, ox-LDLAb in patients with coronary heart disease and its clinical significance].

OBJECTIVE: To detect the levels of index related to inflammation such as soluble CD40 ligand (sCD40L), neutrophil collagenase-8 (MMP-8), and pregnancy associated plasma protein-A (PAPP-A) , lipid peroxidation and autoimmune indexes such as oxidized low density lipoprotein (ox-LDL) and its antibody (ox-LDL Ab) in patients with coronary heart disease, and to investigate its relationship with acute coronary syndrome (ACS). METHODS: Contents of sCD40L, MMP-8, PAPP-A, ox-LDL and ox-LDL Ab in the peripheral blood were measured by enzyme-linked immunosorbent assay from 109 patients with coronary heart disease including 36 acute myocardial infarction (AMI), 38 unstable angina pectoris (UAP), and 35 stable angina pectoris (SAP) and 36 controls without coronary heart disease. RESULTS: The levels of each index in the peripheral blood of ACS patients (including AMI and UAP) were higher than those of SAP patients and controls (P < 0.05), and the difference of each index between UAP group and AMI group in ACS patients had no statistical significance (P > 0.05). The levels of each index of SAP patients, except PAPP-A, were all higher than those of controls (P <0.05). All the indexes were helpful in diagnosis of ACS. The area under the ROC curve of each index is between 0.7 and 0.9. CONCLUSION: The increase of sCD40L, MMP-8, PAPP-A, ox-LDL and ox-LDL Ab levels in peripheral blood may be related to the pathogenesis of ACS, and can be used as potential markers of unstable atherosclerosis plaque.