Hairpin DNA-enabled ratiometric electrochemical aptasensor for detection of malathion.

A hairpin DNA-enabled ratiometric electrochemical aptasensor is reported for sensitive and reliable detection of malathion (MAL). The approach employs hairpin DNA (ferrocene-labeled, Fc-hDNA) as a carrier to hybridize MAL aptamers (methylene blue-labeled, MB-Apt) to form double-stranded DNA structures on an electrode. The presence of MAL induces the removal of aptamers, and hDNA re-forms hairpin structures, causing a decrease in the oxidation current of MB (IMB) and an increase in the oxidation current of Fc (IFc). The ratiometric signal of IFc/IMB responds quantitatively to MAL concentrations. To compare analytical performances, a linear single-stranded DNA (ssDNA) is also used to construct the ssDNA-based aptasensor. We demonstrate that hairpin DNA possessing a rigid two-dimensional structure can improve the assembly efficiency of aptamers and the stability of redox probes. The approach combines the advantages of the ratiometric electrochemical method with hairpin DNA-based conformational switching probes, enabling hDNA-based aptasensor with enhanced sensitivity and reliability, offering a linear range of 0.001 to 1.0 ng mL-1. The platform was applied to detect MAL in lettuce, and the statistical analysis indicated that no significant differences were found between the developed platform and HPLC-MS.

Dual-ratiometric electrochemical aptasensor based on carbon nanohorns/anthraquinone-2-carboxylic acid/Au nanoparticles for simultaneous detection of malathion and omethoate.

Organophosphorus pesticides (OPs) are one of the most frequently used pesticides in agriculture, and their residues in environment have caused serious human health and environmental concerns. In this work, we reported a dual-ratiometric electrochemical aptasensor based on carbon nanohorns/anthraquinone-2-carboxylic acid/Au nanoparticles (CNHs/AQ/AuNPs) for simultaneous detection of malathion (MAL) and omethoate (OMT). Here, CNHs/AQ/AuNPs composites were synthesized by a simple room temperature method, and used as a substrate to generate a reference signal (IAQ) and enlarge response signals. Hairpin DNA was then immobilized, offering independent and specific binding sites to further adsorb MB-labelled MAL aptamer (MB-Apt1) and Fc-labelled OMT aptamer (Fc-Apt2). Upon the addition of MAL or OMT, the formation of aptamer-target complex caused the release of MB-Apt1 or Fc-Apt2 from the electrode, resulting in a decrease in IMB or IFc, while IAQ kept unchanged. Based on this principle, the ratiometric signals of IMB/IAQ and IFc/IAQ were used to simultaneously detect MAL and OMT, offering a linear range of 3 pg mL-1 to 3 ng mL-1 for MAL and 10 pg mL-1 to 10 ng mL-1 for OMT, and no significant cross-reactivity existed. By taking advantage of the excellent conductivity and large specific area of CNHs/AQ/AuNPs and the stable two-dimensional structure of hairpin DNA, the aptasensor exhibited high sensitivity, selectivity and reliability. Our work has offered a novel way for simultaneous detection of multiple OPs.

High-performance compact athermal panoramic annular lens design with separated radial optical power.

A panoramic annular lens can provide abundant spatial information in real time with its compact and scalable structure. To deal with extreme temperature environments and to improve the utilization of an image plane, we propose a high-performance compact athermal panoramic annular lens with separated radial optical power. By introducing multiple free-form surfaces, the system achieves a field of view of (40∘∼100∘)×360∘, an f-number of 2.2, and a total length of 40 mm. The modulation transfer function of the system's full fields of view is greater than 0.5 at 200 lp/mm, and the distortion is less than 2.5%. It can work stably in the range of -30∘C to 70°C. The proposed system is compact, athermal, and high-performing and has broad application prospects in the fields of visual positioning and biomedicine.

Label-free ratiometric homogeneous electrochemical aptasensor based on hybridization chain reaction for facile and rapid detection of aflatoxin B1 in cereal crops.

Aflatoxin B1 (AFB1) contamination has raised global concerns in agricultural and food industry; thus, sensitive, accurate and rapid AFB1 sensors are essential in many circumstances. Herein, we developed a label-free and immobilization-free ratiometric homogeneous electrochemical aptasensor based on hybridization chain reaction (HCR) for facile and rapid determination of AFB1. Methylene blue (MB) and ferrocene (Fc) were used as label-free probes to produce a response signal (IMB) and a reference signal (IFc) in solution phase, respectively. The ratio of IMB/IFc was used as a yardstick to quantify AFB1. HCR was exploited to enlarge the intensity of IMB as well as ratiometric signal. By combining label-free homogeneous assay and ratiometric strategy, the resulting aptasensor offered sensitive, rapid, and reliable determinations of AFB1 with a detection limit of 38.8 pg mL-1. The aptasensor was then used to determine AFB1 in cereal samples with comparable reliability as HPLC-MS.

Design of a compact varifocal panoramic system based on the mechanical zoom method.

A compact varifocal panoramic annular lens (PAL) system based on the four-component mechanical zoom method is proposed, which solves the problem that the traditional PAL system cannot zoom in to the region of interest. By moving the zoom group and the compensation group, our design achieves continuous zooming, in which the focal length changes from 3.8 to 6 mm. It can keep the position of the image surface unchanged while maintaining a compact structure. The system has a field of view (FoV) of 25°-100° in wide-angle mode and an FoV of 25°-65° in telephoto mode. The modulation transfer function of the wide-angle view is higher than 0.22 at 147 lp/mm. The F-theta distortion is less than 3%, and the relative illuminance is higher than 0.9 in the zoom process. Compared with the zoom PAL system with multiple free-form aspheric surfaces, the proposed system uses multiple spherical lenses and only one Q-type asphere lens to achieve outstanding panoramic zoom imaging results. It is practical and straightforward, easy to manufacture, detect, and mass produce.

Low-Cost Hyperspectral Imaging with A Smartphone.

Recent advances in smartphone technologies have opened the door to the development of accessible, highly portable sensing tools capable of accurate and reliable data collection in a range of environmental settings. In this article, we introduce a low-cost smartphone-based hyperspectral imaging system that can convert a standard smartphone camera into a visible wavelength hyperspectral sensor for ca. £100. To the best of our knowledge, this represents the first smartphone capable of hyperspectral data collection without the need for extensive post processing. The Hyperspectral Smartphone's abilities are tested in a variety of environmental applications and its capabilities directly compared to the laboratory-based analogue from our previous research, as well as the wider existing literature. The Hyperspectral Smartphone is capable of accurate, laboratory- and field-based hyperspectral data collection, demonstrating the significant promise of both this device and smartphone-based hyperspectral imaging as a whole.

Hairpin DNA assisted dual-ratiometric electrochemical aptasensor with high reliability and anti-interference ability for simultaneous detection of aflatoxin B1 and ochratoxin A.

The simultaneous detection of multiple mycotoxins in grains is significant due to the enhanced toxicity induced by their synergistic effects. In this work, a dual-ratiometric electrochemical aptasensing strategy for the simultaneous detection of aflatoxin B1 (AFB1) and ochratoxin A (OTA) was developed. Here, an anthraquinone-2-carboxylic acid (AQ)-labelled complementary DNA (cDNA) was used to provide separate and specific binding sites to assemble the ferrocene-labelled AFB1 aptamer (Fc-Apt1) and methylene blue-labelled OTA aptamer (MB-Apt2). The target-induced current ratios of IFc/IAQ and IMB/IAQ were then used to quantitatively relate to AFB1 and OTA, respectively. Following this principle, two types of aptasensors involving the hairpin DNA (hDNA) and linear single-stranded DNA (ssDNA) as the cDNA were fabricated for performance comparisons. The results revealed that hairpin DNA with a rigid 2D structure can greatly improve the assembly and recognition efficiency of the sensing interface, which makes the hDNA-based aptasensor possess high sensitivity, reliability and anti-interference ability. The hDNA-based aptasensor exhibited a detection range of 10-3000 pg mL-1 for AFB1 and 30-10000 pg mL-1 for OTA, respectively, with no observable cross-reactivity. Furthermore, the aptasensor was applied to analyze corn and wheat samples, and the reliability was validated by HPLC-MS/MS. Our work has presented a novel way for fabricating a high-performance aptasensor for simultaneous detection of multiple mycotoxins.

Sensitivity programmable ratiometric electrochemical aptasensor based on signal engineering for the detection of aflatoxin B1 in peanut.

Accurately monitoring of aflatoxin B1 (AFB1), the most hazardous mycotoxin in agricultural products, is essential for the public health, but various testing demands (e.g. detection range, sensitivity) for different samples can be challenging for sensors. Here, we developed a sensitivity-programmable ratiometric electrochemical aptasensor for AFB1 analysis in peanut. Thionine functionalized reduced graphene oxide (THI-rGO) served as reference signal generator, ferrocene-labelled aptamer (Fc-apt) output the response signal. During analysis, the formation of Fc-apt-AFB1 complex led to its stripping from the electrode and faded the current intensity of Fc (IFc), while the current intensity of THI (ITHI) was enhanced. And ratiometric detection of AFB1 was achieved by using the current intensity ratio (ITHI/IFc) as quantitative signal. Compared with ratiometric strategies that highly rely on the labelled aptamers, the proposed strategy could regulate the value of ITHI/IFc by changing the modification of Fc-apt. And the detection sensitivity was found to be closely related to ITHI/IFc. Under the optimal conditions, the fabricated aptasensor with a dynamic range from 0.05-20 ng mL-1 and a detection limit of 0.016 ng mL-1 for AFB1 analysis. Besides, it exhibited excellent selectivity, reliability and reproducibility. The proposed sensitivity-programmable biosensor can be applied to detect various aptamer-recognized mycotoxins in agricultural sensing.

Ratiometric electrochemical aptasensor for ultrasensitive detection of Ochratoxin A based on a dual signal amplification strategy: Engineering the binding of methylene blue to DNA.

A novel ratiometric electrochemical aptasensor was developed for Ochratoxin A (OTA) detection based on the binding of methylene blue (MB) to DNA with a dual signal amplification strategy. The formation of dsDNA structures between ferrocene-labeled complementary DNA (Fc-cDNA), the OTA aptamer, and complementary helper DNA (hDNA) caused Fc away from the electrode, and allowed dsDNA to bind with a certain amount of MB. Here, a small oxidation current of Fc (IFc) and a large oxidation current of MB (IMB) were obtained. In the presence of OTA, its specific recognition with the aptamer induced the release of aptamer and hDNA from the electrode and subsequently the formation of hairpin structure for cDNA, which caused Fc close to the electrode and a weaker binding ability with MB. Then, an increased IFc and a decreased IMB were obtained. Based on this principle, OTA could be accurately quantified by measuring the ratiometric signal of IFc/IMB. Herein, the dual signal amplification strategy of the introduction of hDNA and the binding with MB after the OTA recognition was exploited to amplify the response signal. The obtained aptasensor showed a linear detection range from 10 pg mL-1 to 10 ng mL-1 and a detection limit of 3.3 pg mL-1. The aptasensor was successfully applied to determine OTA in wheat, and the results were validated through HPLC-MS. Furthermore, by changing the target aptamers, this strategy could be universally used for the determination of various mycotoxins, showing promising potential applications for mycotoxins monitoring in agricultural products and foods.

An ultra-sensitive aptasensor based on carbon nanohorns/gold nanoparticles composites for impedimetric detection of carbendazim at picogram levels.

We reported a carbon nanohorns/gold nanoparticles composites-based impedimetric aptasensor for carbendazim (CBZ) detection in lettuce and orange juice at picogram levels. The increased electron-transfer resistance, resulting from the formation of CBZ-aptamer complex, was recorded by electrochemical impedance spectroscopy as the aptasensor response for CBZ. Under the optimal conditions, the proposed aptasensor displayed a linear response for CBZ ranging from 1 to 1000 pg mL-1 with a detection limit of 0.5 pg mL-1. Noteworthy, the as-developed aptasensor displayed the lowest detection limit for CBZ among the previously reported methods. Common pesticides (atrazine, thiamethoxam, etc.) with 100-fold concentration did not interfere the CBZ detection. For CBZ detection in lettuce and orange juice, satisfactory recoveries were obtained with standard addition method. Statistics demonstrated that no significant differences were found between the data provided by standard HPLC-MS reference method and developed aptasensing method in term of accuracy and precision. We believe that the proposed aptasensor possesses a potential application for CBZ monitoring in agricultural product and food.

Quantitative traceable temperature measurement using novel thermal imaging camera.

Conventional thermal imaging cameras, based on focal-plane array (FPA) sensors, exhibit inherent problems: such as stray radiation, cross-talk and the calibration uncertainty of ensuring each pixel behaves as if it were an identical temperature sensor. Radiation thermometers can largely overcome these issues, comprising of only a single detector element that can be optimised and calibrated. Although the latter approach can provide excellent accuracy for single-point temperature measurement, it does not provide a temperature image of the target object. In this work, we present a micromechanical systems (MEMS) mirror and silicon (Si) avalanche photodiode (APD) based single-pixel camera, capable of producing quantitative thermal images at an operating wavelength of 1 µm. This work utilises a custom designed f-theta wide-angle lens and MEMS mirror, to scan +/- 30° in both x- and y-dimensions, without signal loss due to vignetting at any point in the field of view (FOV). Our single-pixel camera is shown to perform well, with 3 °C size-of-source effect (SSE) related temperature error and can measure below 700 °C whilst achieving ± 0.5 °C noise related measurement uncertainty. Our measurements were calibrated and traceable to the International Temperature Scale of 1990 (ITS-90). The combination of low SSE and absence of vignetting enables quantitative temperature measurements over a spatial field with measurement uncertainty at levels lower than would be possible with FPA based thermal imaging cameras.

Quantitative thermal imaging using single-pixel Si APD and MEMS mirror.

Accurate quantitative temperature measurements are difficult to achieve using focal-plane array sensors. This is due to reflections inside the instrument and the difficulty of calibrating a matrix of pixels as identical radiation thermometers. Size-of-source effect (SSE), which is the dependence of an infrared temperature measurement on the area surrounding the target area, is a major contributor to this problem and cannot be reduced using glare stops. Measurements are affected by power received from outside the field-of-view (FOV), leading to increased measurement uncertainty. In this work, we present a micromechanical systems (MEMS) mirror based scanning thermal imaging camera with reduced measurement uncertainty compared to focal-plane array based systems. We demonstrate our flexible imaging approach using a Si avalanche photodiode (APD), which utilises high internal gain to enable the measurement of lower target temperatures with an effective wavelength of 1 µm and compare results with a Si photodiode. We compare measurements from our APD thermal imaging instrument against a commercial bolometer based focal-plane array camera. Our scanning approach results in a reduction in SSE related temperature error by 66 °C for the measurement of a spatially uniform 800 °C target when the target aperture diameter is increased from 10 to 20 mm. We also find that our APD instrument is capable of measuring target temperatures below 700 °C, over these near infrared wavelengths, with D* related measurement uncertainty of ± 0.5 °C.

Design and realization of a wide field of view infrared scanning system with an integrated micro-electromechanical system mirror.

We present a wide field of view (FOV) infrared scanning system, designed for single-pixel near-infrared thermal imaging. The scanning system consisted of a two-axis micro-electromechanical system (MEMS) mirror that was incorporated within the lens. The optical system consisted of two groups of lenses and a silicon avalanche photodiode. The system was designed for both the production of thermal images and also to utilize the techniques of radiation thermometry to measure the absolute temperature of targets from 500°C to 1100°C. Our system has the potential for real-time image acquisition, with improved data acquisition electronics. The FOV of our scanning system was ±30° when fully utilizing the MEMS mirror's scanning angle of ±5°. The pixel FOV (calculated from the distance to target size ratio) was 100:1. The image quality was analyzed, including the modulation transfer function, spot diagrams, ray fan plots, lateral chromatic aberrations, distortion, relative illumination, and size-of-source effect. The instrument was fabricated in our laboratory, and one of the thermal images, which was taken with the new lens, is presented as an example of the instrument optical performance.

Superior catalytic activity of Pt/carbon nanohorns nanocomposites toward methanol and formic acid oxidation reactions.

Pt nanoparticle-loaded carbon nanohorns (Pt/CNHs) nanocomposites were synthesized by using formic acid as reducing agent at room temperature. Taking the advantage of the high surface area and excellent electronic conductivity, CNHs were used as support without any pretreatments for the enhancement of catalytic performance. By adjusting the feeding amount of H2PtCl6, the mass, size and distribution of Pt nanoparticles could be effectively controlled on CNHs. Compared with commercial Pt/C, the synthesized Pt/CNHs exhibit higher catalytic activity and improved long-term stability toward both methanol and formic acid oxidations. Among the Pt/CNHs, 13% wt. Pt/CNHs exhibit the best catalytic performances for the small size (2.4 nm) and uniform distribution of Pt NPs on CNHs. Our work reveals the superior catalytic performance of Pt/CNHs which may be a promising substitute for commercial Pt/C.