[Discussion of grading method of small opacity profusion of pneumoconiosis on CT scans and the corresponding reference images].

Objective: To explore the CT grading method of small opacity profusion of pneumoconiosis, and draw up the corresponding CT reference film. Methods: In December 2019, Three hundred thirty-seven cases of pneumoconosis and suspected pneumoconiosis were examined by chest radiography and Computed Tomography (CT) in the same period. According to Diagnosis of Occupational Pneumoconiosis (GBZ 70-2015) , small opacity profusion of pneumoconiosis in each zone of lung was divided. On CT scans, it was divided into 5 grades of 0, 0+, 1, 2 and 3. Grade 0 corresponded to Sub-grade 0/- and Sub-grade 0/0 of Grade 0 in chest radiograph. Grade 0+ was equivalent to Sub-grade 0/1 of Grade 0. Grade 1, 2, 3 were equivalent to Grade 1, 2 and 3, respectively (including each sub-grade) . The CT image quality of each zone of lung was divided into 1 to 4 levels. Results of level 4 were not included in statistical analyses.Based on the results of small opacity profusion in each zone of lung, consistency analysis was performed between chest radiograph and CT. The selection method of reference films was developed. Based on the types and grades of small opacity, the final reference films were determined. Results: There were 1877 zones of lung with CT image quality from level 1 to 3, including 335 in upper right, 319 in middle right, 284 in lower right, 334 in upper left, 320 in middle left and 286 in lower left. The Kappa values of small opacity profusion in upper right zone, upper left zone, left middle zone, and lower left zone were all between 0.4-0.75. In middle right zone and lower right zone, they were all above 0.75.Among all 6 zones of lung, the diagnostic concordance rates between CT and chest radiograph were all above 80%.The corresponding CT reference films were proposed, including type p and q in Grade 2 and 3, type r in Grade 2, type s and t in Grade 0+ to 3. Conclusion: The CT grading method for small opacity profusion of pneumoconiosis is feasible, and the application value of its reference films needs to be further verified.

Adjacent SNPs in the transcriptional regulatory region of the FADS2 gene associated with fatty acid and growth traits in chickens.

Delta-6 fatty acid desaturases are rate-limiting desaturases involved in metabolic processes of fatty acids, and they are encoded by the FADS2 gene. In the current study, an F2 resource population of Gushi chickens crossed with Anak broilers was used to investigate the genetic effects of the chicken FADS2. Two adjacent single nucleotide polymorphisms (SNPs) (g.4290C>G and g.4291C>A) were identified in the transcriptional regulatory region of the FADS2 gene by means of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and created restriction site-PCR-RFLP. Associations between the two SNPs with chicken fatty acid contents and growth traits were determined using linkage disequilibrium, haplotype construction, and association analysis. The two SNPs and their haplotype combinations were significantly associated with linoleic acid (C18:2), α-linolenic acid (C18:3), arachidonic acid (C20:4), body weight (BW)2, BW4, BW6, shank girth (SG)4, and breast bone length 4 (P<0.05). These results suggested that the SNPs of the FADS2 gene affected the content of essential fatty acid in muscle, and played a role in the early-stage growth rate of chickens.

A novel 31-bp indel in the paired box 7 (PAX7) gene is associated with chicken performance traits.

1. A novel 31-bp indel polymorphism in intron 3 of the chicken paired box 7 (PAX7) gene was identified and genotyped in an F2 resource population of Gushi chicken crossed with Anka broiler to analyse its associations with chicken growth, carcass and meat quality traits. 2. Results showed that the 31-bp indel was significantly associated with body weight at 4, 6, 8, 10, 12 weeks of age and body size indices including shank length, shank girth, body slanting length at 8 and 12 weeks of age. Significant associations were found for carcass weight, semi-evisceration weight, evisceration weight, breast muscle fibre diameter, leg muscle fibre diameter, breast muscle fibre density, while no significant association with leg muscle fibre density was observed. 3. It was concluded that the 31-bp indel in intron 3 of the PAX7 gene was associated with chicken growth, carcass and meat quality traits where the 31-bp deletion had a negative effect on chicken growth and carcass traits and positive effect on meat quality traits.

Two novel SNPs of the 3-hydroxy-3-methylglutaryl coenzyme A reductase gene associated with growth and meat quality traits in the chicken.

The enzyme 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR) is rate-limiting for metabolism of cholesterol; it plays an important role in endogenous cholesterol biosynthesis. We used DNA sequencing technology and created restriction site PCR-RFLP to detect HMGCR SNPs in an F(2) resource population of Gushi chicken and Anka broilers. We found a G/T mutation (Gln/His) in exon 17 and a T/C mutation (Pro/Pro) in exon 18. Based on association analysis of these HMGCR polymorphisms in 864 Gushi/Anka F(2) hybrids, these two mutations have significant effects on growth, carcass, meat quality, and lipid concentration.

Differential associations of body mass index and adiposity with all-cause mortality among men in the first and second National Health and Nutrition Examination Surveys (NHANES I and NHANES II) follow-up studies.

OBJECTIVE: The frequently observed U-shaped relationship between body mass index (BMI; kg/m(2)) and mortality rate may be due to the opposing effects of fat mass (FM) and fat-free mass (FFM) components of BMI on mortality rate. The purpose is to test the hypothesis stated above. DESIGN: Longitudinal prospective cohort studies. The mortality follow-up of the first and second National Health and Nutrition Examination Surveys (NHANES I and NHANES II). SUBJECTS: A total of 10 169 male subjects aged 25-75 who participated in NHANES I and II were selected for analyses. Follow-up continued until 1992. The mean follow-up time was 14.6 y for NHANES I and 12.9 y for NHANES II. Ninety-eight percent of the participants were successfully followed representing a total of 3722 deaths. MEASUREMENTS: Subscapular and triceps skinfolds thickness were used as FM indicators, whereas upper arm circumference was used as a FFM indicator. The Cox proportional hazards model tested the relationships of BMI, FM and FFM with all-cause mortality adjusting for age, smoking status, race and education levels. RESULTS: BMI had a U-shaped relationship with mortality, with a nadir of approximately 27 kg/m(2). However, when indicators of FM and FFM were added to the model, the relationship between BMI and mortality became more nearly monotonic increasing. Moreover, the relationship between FM indicator and mortality was monotonic increasing and the relationship between FFM indicator and mortality was monotonic decreasing. CONCLUSION: These results support the hypothesis that the apparently deleterious effects of marked thinness may be due to low FFM and that, over the observed range of the data, marked leanness (as opposed to thinness) has beneficial effects.

Effect of vibration magnitude and repetitive exposure on finger blood flow in healthy subjects.

In order to study the effect of the magnitude of vibrations and repetitive exposure on finger blood flow, we exposed ten healthy subjects to three experimental conditions: (1) vibration of 3.16 m/s2 at 60 Hz, (2) vibration of 31.6 m/s2 at 60 Hz, and (3) no vibration, as a control. Under the experimental conditions, the right hand was exposed to 5-min vibration three times, with intervening 5-min rests. Meanwhile, the blood flows of both middle fingers were continuously measured with a blood flow meter based on the thermal diffusion method. Finger blood flow was significantly decreased in both hands with exposure to vibrations of 3.16 m/s2 and 31.6 m/s2. Increased magnitude of vibration tended to enhance the decrease in finger blood flow in both hands, and repeated exposure to vibration had cumulative effects on the decrease in finger blood flow in the unexposed left hand.

Digital sensory nerve conduction velocity and vibration perception threshold in peripheral neurological test for hand-arm vibration syndrome.

To evaluate peripheral neuropathy in patients with vibration syndrome, an examination was conducted of sensory nerve conduction velocity (SCV) in the digital segment of the median nerve in the middle finger and vibration perception threshold (VPT) at 125 Hz on the same middle fingertip. In addition, possible correlations were investigated between the two measurements. SCVs in the digital segment were measured by stimulating at the wrist electrically and recording from two pairs of electrodes in the finger. Fractionated SCVs were also measured in the palm-to-finger, wrist-to-palm, and elbow-to-wrist segments. The subjects were 52 patients with vibration syndrome and 40 healthy controls of similar age. SCVs in the digital segment and the wrist-to-palm segment were significantly slower in the patients than in the controls, and VPTs were higher in the patients. The strongest correlation of VPTs with SCVs among nerve segments measured was shown in the digital segment. With an increase in VPTs, SCVs in the digital segment tended to be slower, and slowed digital SCVs were encountered more frequently: 13% in VPTs below 5.0 dB and 56% in VPTs above 17.5 dB. Slowed digital SCVs were found in 43% of the patients and increased VPTs were encountered in 92%.

Knee pain and its associations with age, sex, obesity, occupation and living conditions in rural inhabitants of Japan.

Factors associated with knee pain were investigated using 871 self-administered questionnaires (405 men and 466 women) from residents aged over 30 years in a rural area of Japan. The prevalence of knee pain increased with age, particularly in women over 50. It was significantly higher in women than in men. Body mass index (BMI) was significantly related to knee pain in women, though not in men. With reference to occupational factors, frequent heavy lifting on the job was significantly associated with knee pain in both men and women, whereas job-related standing and walking showed no such relationship. As for living conditions, residence on rather steep mountain slopes and the habit of sitting on Japanese tatami mats were significant factors related to knee pain in men, but not in women. There was no association of knee pain with the style of toilet (Japanese or Western). After controlling for all significant factors by multiple logistic regression analysis, age, heavy lifting, and residence on steep slopes were found to be independent factors related to knee pain in men; and age, BMI, and heavy lifting were the factors in women.

Digital nerve conduction velocity for evaluation of peripheral nerve impairments in vibration syndrome.

In order to evaluate peripheral nerve impairments in vibration syndrome, sensory nerve conduction velocity (SCV) in the digital segment of the median nerve was measured by applying electrical stimulation to the wrist and recording from two pairs of electrodes attached to the middle finger. Fractionated SCVs were also measured in the palm-to-finger, wrist-to-palm, and elbow-to-wrist segments of the median nerve. Subjects were 65 patients with vibration syndrome and 48 healthy controls of similar age. SCVs in the digital nerve segment were significantly slower in the patients than in the controls. Significant slowing was also found in the wrist-to-palm segment. SCVs in the palm-to-finger and elbow-to-wrist segments did not differ between the two groups. Slowed SCVs were most frequently encountered in the digital segment (38% of the patients). The slowing in the wrist-to-palm segment was found in 20% of the patients. Present findings indicate that the digital segment is most frequently affected in vibration-induced neuropathy, and measurement of SCVs in the digital segment would serve to evaluate the peripheral neuropathy in vibration syndrome.

Analysis of various factors affecting vibration-induced white finger.

Forty-one stone quarriers were studied with regard to various factors affecting vibration-induced white finger (VWF), i. e., age, total operating time (TOT), body mass index (BMI), smoking, drinking, past history of diseases and sensitivity to cold in childhood. Subjects were asked about these factors by interview according to a questionnaire. TOT and BMI were significantly different between subjects with VWF (VWF group) and those without VWF (NON-VWF group). TOT was considered to be a great factor in stone quarriers. BMI was 21.6 (kg/m2) in VWF group against 24.0 (kg/m2) in NON-VWF group, which suggests that lean subjects may be susceptible to VWF. As for other factors such as age, smoking, drinking, past history of diseases and sensitivity to cold in childhood, our findings showed no significant difference between VWF group and NON-VWF group.