Clinical and genetic study of ABCB4 gene-related cholestatic liver disease in China: children and adults.

BACKGROUND: ABCB4 gene-related cholestatic liver diseases have a wide spectrum of clinical and genetic variations. The correlation between genotype and clinical phenotype still unclear. This study retrospectively analyzed the clinical and pathological characteristics of 23 patients with ABCB4 gene-related cholestatic liver diseases. Next-generation sequencing was used to identify the genetic causes. RESULTS: The 23 included patients (15 children and 8 adults) were diagnosed as progressive familial intrahepatic cholestasis type 3 (PFIC3), drug-induced liver injury (DILI), cirrhosis cholestasis, cirrhosis, and mild liver fibrosis. Nineteen patients underwent liver pathological examination of the liver, exhibiting fibrosis, small bile duct hyperplasia, CK7(+), Cu(+), bile duct deletion, and cirrhosis. Thirty ABCB4 variants were identified, including 18 novel variants. CONCLUSION: ABCB4 gene-related cholestatic liver diseases have a wide spectrum of clinical and genetic variations. Biallelic ABCB4 mutation carriers tended to severe PFIC3, which mostly occurs in children; while ABCB4 non-biallelic variants can lead to milder ICP, LACP, DILI or overlapping, mostly in adults. Thus, the ABCB4 genotype has a specific correlation with the phenotype, but there are exceptions. Non-biallelic null mutations can cause severe diseases. The mechanisms underlying this genetic phenotype require further investigation.

One-step synthesis of a core-shell structured biochar using algae (Chlorella) powder and ferric sulfate for immobilizing Hg(II).

Mercury (Hg) pollution poses a significant environmental challenge. One promising method for its removal is the sorption of mercuric ions using biochar. FeS-doped biochar (FBC) exhibits effective mercury adsorption, however may release excess iron into the surrounding water. To address this issue, a novel magnetic pyrrhotite/magnetite-doped biochar with a core-shell structure was synthesized for the adsorption of 2-valent mercury (Hg(II)). The proposed synthesis process involved the use of algae powder and ferric sulfate in a one-step method. By varying the ratio of ferric sulfate and alga powder (within the range of 0.18 - 2.5) had a notable impact on the composition of FBC. As the ferric sulfate content increased, the FBC exhibited a higher concentration of oxygen-containing groups. To assess the adsorption capacity, Langmuir and Freundlich adsorption models were applied to the experimental data. The most effective adsorption was achieved with FBC-4, reaching a maximum capacity (Qm) of 95.51 mg/g. In particular, at low Hg(II) concentrations, FBC-5 demonstrated the ability to reduce Hg(II) concentrations to less than 0.05 mg/L within 30 min. Additionally, the stability of FBC was confirmed within the pH range of 3.8 - 7.2. The study also introduced a model to analyze the adsorption preference for different Hg(II) species. Calomel was identified in the mercury saturated FBC, whereas the core-shell structure exhibited excellent conductivity, which most likely contributed to the minimal release of iron. In summary, this research presents a novel and promising method for synthesizing core-shell structured biochar and provides a novel approach to explore the adsorption contribution of different metal species.

Boosting Ammonium Oxidation in Wastewater by the BiOCl-Functionalized Anode.

Mixed metal oxide (MMO) anodes are commonly used for electrochlorination of ammonium (NH4+) in wastewater treatment, but they suffer from low efficiency due to inadequate chlorine generation at low Cl- concentrations and sluggish reaction kinetics between free chlorine and NH4+ under acidic pH conditions. To address this challenge, we develop a straightforward wet chemistry approach to synthesize BiOCl-functionalized MMO electrodes using the MMO as an efficient Ohmic contact for electron transfer. Our study demonstrates that the BiOCl@MMO anode outperforms the pristine MMO anode, exhibiting higher free chlorine generation (24.6-60.0 mg Cl2 L-1), increased Faradaic efficiency (75.5 vs 31.0%), and improved rate constant of NH4+ oxidation (2.41 vs 0.76 mg L-1 min-1) at 50 mM Cl- concentration. Characterization techniques including electron paramagnetic resonance and in situ transient absorption spectra confirm the production of chlorine radicals (Cl• and Cl2•-) by the BiOCl/MMO anode. Laser flash photolysis reveals significantly higher apparent second-order rate constants ((4.3-4.9) × 106 M-1 s-1 at pH 2.0-4.0) for the reaction between NH4+ and Cl•, compared to the undetectable reaction between NH4+ and Cl2•-, as well as the slower reaction between NH4+ and free chlorine (102 M-1 s-1 at pH < 4.0) within the same pH range, emphasizing the significance of Cl• in enhancing NH4+ oxidation. Mechanistic studies provide compelling evidence of the capacity of BiOCl for Cl- adsorption, facilitating chlorine evolution and Cl• generation. Importantly, the BiOCl@MMO anode exhibits excellent long-term stability and high catalytic activity for NH4+-N removal in a real landfill leachate. These findings offer valuable insights into the rational design of electrodes to improve electrocatalytic NH4+ abatement, which holds great promise for wastewater treatment applications.

Functional Cure of Chronic Hepatitis B with Antiviral Treatment in Children having High-level Viremia and Normal or Mildly Elevated Serum Aminotransferase.

Background and Aims: There is a lack of data supporting the notion that antiviral treatments can benefit children with chronic hepatitis B (CHB) having high viremia and normal or mildly elevated serum alanine aminotransferase (ALT) levels. We aimed to analyze the efficacy of antiviral treatments in children with CHB and explore the factors associated with functional cure. Methods: Forty-eight children with CHB having high viremia and normal or mildly elevated serum ALT levels were screened in this real-world study. Thirty-two children received either interferon-alpha (IFN-α) monotherapy, IFN-α therapy with a nucleoside analog (NA) add-on, or IFN-α and NA combination therapy. The 16 children in the control group did not receive antiviral treatment. All 48 children were available for follow-up assessments for the entire 36-month study period. We identified a functional cure with respect to hepatitis B virus (HBV) DNA loss, loss /seroconversion of circulating hepatitis B e antigen (HBeAg), and loss of hepatitis B surface antigen (HBsAg) with or without seroconversion. Cox regression analysis was employed to evaluate the factors that may have influenced the functional cure. Results: After 36 months, the cumulative functional cure rate was 56.25% (18/32) in the treated group and 0% (0/16) in the control group (p<0.001). In the treated group, the serum HBV DNA levels declined rapidly at the end of a 6-month visit and the cured children achieved a loss rate of 100% (18/18) within 16 months of beginning treatment, compared with 64.29% (9/14) of the uncured children (p<0.001). The rates of HBeAg seroconversion were significantly higher among the cured children than among the uncured children (p<0.001). All 16 children in the control group maintained high levels of serum HBV DNA and were positive for both serum HBeAg and HBsAg during the entire 36 months of the study period. Functional cure was associated with younger ages (1-6 vs. 7-14 years, p=0.013), CD8+ T lymphocyte counts (p=0.013), and B lymphocyte counts (p=0.003). No serious adverse events were observed. Conclusions: Antiviral treatment achieved a functional cure of CHB in a high proportion of children having high-level viremia and normal or mildly elevated ALT levels. Younger age and high peripheral lymphocyte counts were associated with this functional cure.

New Insights into the Role of Natural Organic Matter in Fe-Cr Coprecipitation: Importance of Molecular Selectivity.

Coprecipitation of Fe/Cr hydroxides with natural organic matter (NOM) is an important pathway for Cr immobilization. However, the role of NOM in coprecipitation is still controversial due to its molecular heterogeneity and diversity. This study focused on the molecular selectivity of NOM toward Fe/Cr coprecipitates to uncover the fate of Cr via Fourier transform-ion cyclotron resonance-mass spectrometry (FT-ICR-MS). The results showed that the significant effects of Suwannee River NOM (SRNOM) on Cr immobilization and stability of the Fe/Cr coprecipitates did not merely depend on the adsorption of SRNOM on Fe/Cr hydroxides. FT-ICR-MS spectra suggested that two pathways of molecular selectivity of SRNOM in the coprecipitation affected Cr immobilization. Polycyclic aromatics and polyphenolic compounds in SRNOM preferentially adsorbed on the Fe/Cr hydroxide nanoparticles, which provided extra binding sites and promoted the aggregation. Notably, some specific compounds (i.e., polyphenolic compounds and highly unsaturated phenolic compounds), less unsaturated and more oxygenated than those adsorbed on Fe/Cr hydroxide nanoparticles, were preferentially incorporated into the insoluble Cr-organic complexes in the coprecipitates. Kendrick mass defect analysis revealed that the insoluble Cr-organic complexes contained fewer carbonylated homologous compounds. More importantly, the spatial distribution of insoluble Cr-organic complexes was strongly related to Cr immobilization and stability of the Fe/Cr-NOM coprecipitates. The molecular information of the Fe/Cr-NOM coprecipitates would be beneficial for a better understanding of the transport and fate of Cr and exploration of the related remediation strategy.

Selective activation of peroxymonosulfate govern by B-site metal in delafossite for efficient pollutants degradation: Pivotal role of d orbital electronic configuration.

Radical and non-radical oxidation pathways have been universally validated in transition metals (TMs) oxides activated peroxymonosulfate (PMS) processes. However, achieving high efficiency and selectivity of PMS activation remains challenging due to the ambiguous tuning mechanism of TMs sites on PMS activation in thermodynamic scope. Herein, we demonstrated that the exclusive PMS oxidation pathways were regulated by d orbital electronic configuration of B-sites in delafossites (CuBO2) for Orange I degradation (CoIII 3d6 for reactive oxygen species (ROSs) vs. CrIII 3d3 for electron transfer pathway). The d orbital electronic configuration was identified to affect the orbital overlap extent between 3d of B-sites and O 2p of PMS, which induced B-sites offering different types of hybrid orbital to coordinate with O 2p of PMS, thereby forming the high-spin complex (CuCoO2@PMS) or the low-spin complex (CuCrO2@PMS), on which basis PMS was selectively dissociated to form ROSs or achieve electron transfer pathway. As indicated by thermodynamic analysis, a general rule was proposed that B-sites of less than half-filled 3d orbital tended to act as electron shuttle, i.e., CrIII (3d3), MnIII (3d4), interacting with PMS to execute an electron transfer pathway for degrading Orange I, while B-sites of between half-filled and full-filled 3d orbital preferred to be electron donator, i.e., CoIII (3d6), FeIII (3d5), activating PMS to generate ROSs. These findings lay a foundation for the oriented design of TMs-based catalysts from the atomic level according to d orbital electronic configuration optimization, as so to facilitate the achievement of PMS-AOPs with highly selective and efficient remediation of contaminants in water purification practice.

Physicochemical properties of nanosized biochar regulated by heat treatment temperature dictates algal responses: From the perspective of fatty acid metabolism.

Nanosized biochar (NBC) is an important fraction of biochar (BC) as it can exert nano-scale effects on aquatic organisms, attracting increasing research attention. However, effects of different physicochemical properties of NBC on biological responses at the metabolic and gene expression level are not comprehensively understood. Here, biological effects of NBCs pyrolyzed at different heat treatment temperatures (HTTs, 350-700 °C) were evaluated using freshwater algae Chlorella vulgaris, from the perspectives of growth and fatty acid (FA) profile changes. NBC pyrolyzed at 700 °C (N700) induced the greatest algal growth inhibition and oxidative stress than N350 and N500. In addition, NBC exposure to 50 mg/L increased saturated and monounsaturated FAs, along with a decrease in polyunsaturated FAs (PUFAs). Exposure to NBC also significantly influenced the expression of key FA metabolism genes (3fad, sad, kasi and accd), demonstrating the potential role of reactive oxygen species-mediated PUFA reduction accompanied by increased membrane permeability in algal toxicity upon NBC exposure. The observed differences in response to N700 were attributed to its smaller particle size and higher abundance of -COOH. These findings reveal the underlying mechanisms in the algal response to NBCs and provide valuable guidance for the safe design and application of BC materials.

Effects of biochar on anaerobic treatment systems: Some perspectives.

Many anaerobic activities involve carbon, nitrogen, iron, and sulfur cycles. As a well-developed porous material with abundant functional groups, pyrolytic biochar has been widely researched in efforts to promote microbial activities. However, the lack of consensus on the biochar mechanism has limited its practical application. This review summarizes the effects of different pyrolysis temperatures, particle sizes, and dosages of biochar on microbial activities and community in Fe(III) reduction, anaerobic digestion, nitrogen removal, and sulfate reduction systems. It was found that biochar could promote anaerobic activities by stimulating electron transfer, alleviating toxicity, and providing suitable habitats for microbes. However, it inhibits microbial activities by releasing heavy metal ions or persistent free radicals and adsorbing signaling molecules. Finding a balance between the promotion and inhibition of biochar is therefore essential. This review provides valuable perspectives on how to achieve efficient and stable use of biochar in anaerobic systems.

Host-Endosymbiont Relationship Impacts the Retention of Bacteria-Containing Amoeba Spores in Porous Media.

Amoebae are protists that are commonly found in water, soil, and other habitats around the world and have complex interactions with other microorganisms. In this work, we investigated how host-endosymbiont interactions between amoebae and bacteria impacted the retention behavior of amoeba spores in porous media. A model amoeba species, Dictyostelium discoideum, and a representative bacterium, Burkholderia agricolaris B1qs70, were used to prepare amoeba spores that carried bacteria. After interacting with B. agricolaris, the retention of D. discoideum spores was enhanced compared to noninfected spores. Diverse proteins, especially proteins contributing to the looser exosporium structure and cell adhesion functionality, are secreted in higher quantities on the exosporium surface of infected spores compared to that of noninfected ones. Comprehensive examinations using a quartz crystal microbalance with dissipation (QCM-D), a parallel plate chamber, and a single-cell force microscope present coherent evidence that changes in the exosporium of D. discoideum spores due to infection by B. agricolaris enhance the connections between spores in the suspension and the spores that were previously deposited on the collector surface, thus resulting in more retention compared to the uninfected ones in porous media. This work provides novel insight into the retention of amoeba spores after bacterial infection in porous media and suggests that the host-endosymbiont relationship regulates the fate of biocolloids in drinking water systems, groundwater, and other porous environments.

Aqueous aggregation and deposition kinetics of fresh and carboxyl-modified nanoplastics in the presence of divalent heavy metals.

The presence of heavy metals alters the colloidal stability and deposition of nanoplastics (NPs) in urban waters. Such processes are important to assess the mobility and fate of NPs and their associated heavy metals. Up to date, few studies have reported the impact of heavy metals on the colloidal behaviors of NPs and the involved mechanisms. In the study, time-resolved dynamic light scattering (DLS) and quartz crystal microbalance with dissipation (QCM-D) methods were used to assess the aggregation and deposition kinetics of polystyrene nanospheres with divalent heavy metals. For comparison, carboxyl-modified polystyrene nanospheres were used. Results reveal that heavy metals destabilized NPs more significantly than calcium ions. Spectroscopy and transmission electron microscopy analysis propose that heavy metals destabilized NPs via inner-sphere coordination with carboxyl groups and cation-π interactions, further leading to the formation of different dimensional aggregates. QCM-D results suggest that the deposition rate, irreversibility, and film compactness of NPs on silica surfaces first increased but further decreased as heavy metal concentration increased. Such deposition behaviors depended on the bridging effects between NPs and silica and aggregation-induced diffusion limitation. In that case, the destabilization and retention ability of heavy metals for NPs were related to their electronegativity and hydration shell thickness. In urban waters, the presence of natural organic matter (NOM) decreased the destabilization and retention ability of heavy metals, whereas heavy metals with environmentally relevant concentrations still enhanced the aggregation and deposition of NPs compared with other environmental cations. This study highlights the impact of heavy metal property on the colloidal behaviors of NPs, thus deepening our understanding of the mobility and fate of NPs associated with heavy metals in urban waters.

Oxygen Vacancy-Induced Nonradical Degradation of Organics: Critical Trigger of Oxygen (O2) in the Fe-Co LDH/Peroxymonosulfate System.

Ubiquitous oxygen vacancies (Vo) existing in metallic compounds can activate peroxymonosulfate (PMS) for water treatment. However, under environmental conditions, especially oxygenated surroundings, the interactions between Vo and PMS as well as the organics degradation mechanism are still ambiguous. In this study, we provide a novel insight into the PMS activation mechanism over Vo-containing Fe-Co layered double hydroxide (LDH). Experimental results show that Vo/PMS is capable of selective degradation of organics via a single-electron-transfer nonradical pathway. Moreover, O2 is firstly demonstrated as the most critical trigger in this system. Mechanistic studies reveal that, with abundant electrons confined in the vacant electron orbitals of Vo, O2 is thermodynamically enabled to capture electrons from Vo to form O2•- under the imprinting effect and start the activation process. Simultaneously, Vo becomes electron-deficient and withdraws the electrons from organics to sustain the electrostatic balance and achieve organics degradation (32% for Bisphenol A without PMS). Different from conventional PMS activation, under the collaboration of kinetics and thermodynamics, PMS is endowed with the ability to donate electrons to Vo as a reductant other than an oxidant to form 1O2. In this case, 1O2 and O2•- act as the indispensable intermediate species to accelerate the circulation of O2 (as high as 14.3 mg/L) in the micro area around Vo, and promote this nano-confinement electron-recycling process with 67% improvement of Bisphenol A degradation. This study provides a brand-new perspective for the nonradical mechanism of PMS activation over Vo-containing metallic compounds in natural environments.

Long-term benefits of interferon-α therapy in children with HBeAg-positive immune-active chronic hepatitis B.

The long-term benefits of interferon-α (IFN-α) treatment in children with chronic hepatitis B (CHB) remain unclear. We conducted a retrospective and real-world study to evaluate the safety and long-term clearance rates of hepatitis B e antigen (HBeAg) and hepatitis B surface antigen (HBsAg) in CHB children who received IFN-α monotherapy for 72 weeks and were with 13-year follow-up visit. Participants treated with IFN-α (n = 316) were more likely to become HBeAg negatve (39.87% vs. 27.37%; p < .05) and HBsAg negative (11.08% vs. 3.16%; p < .05) by the end of the treatment period than untreated participants (n = 95). Treated participants also had higher cumulative rates of HBeAg loss (74.13% vs. 59.27%; p < .05) and HBsAg loss (46.95 vs. 33.11%; p < 0.05) than untreated participants in parallel by the end of 13-year follow-up. In particular, the cumulative rate of HBsAg loss was higher in treated children aged 1-7 years than in those aged 8-17 years (71.40% vs. 39.0%; p < .01). Children who were HBeAg-negative at the end of IFN-α treatment or who had serum alanine aminotransferase levels of ≥80 IU/L at baseline were likely to have higher cumulative HBsAg loss rates. Accordingly, HBeAg loss at 72 weeks was positively associated with the cumulative HBsAg loss rate in untreated children. There were no serious adverse events of IFN-α therapy for the treated patients throughout the study period. Overall, IFN-α therapy was effective in obtaining higher long-term cumulative rates of HBeAg and HBsAg loss in children with HBeAg-positive immune-active CHB, especially among those aged 1-7 years.

Overlooked contributions of biochar-derived dissolved organic matter on the adsorption of Pb (â ¡): Impacts of fractionation and interfacial force.

Comprehensive understanding of how the release of biochar-derived dissolved organic matter (BDOM) affects the immobilization of heavy metals when biochar (BC) is applied for long-term soil remediation is extremely important. In this study, BCs prepared under different pyrolysis temperatures were fractionated into residual BC (RBC), nano-sized BC (NBC), and BDOM, in order to clarify the contribution of BDOM for lead (Pb(II)) adsorption on BC and to explore the interfacial mechanisms. Results demonstrated that the adsorption capacity (Qe) of Pb(II) on BC improved from 166.1 to 423.9 mg g-1 with the increase in the pyrolysis temperature from 350 to 800 °C. The sum of Qe of Pb(II) on NBC and RBC was lower than that on BC, due to the complexation between BDOM and Pb(II) rather than pH variance and cation exchange. Ultraviolet-visible and fluorescence spectroscopy revealed that fulvic-like substances as well as small molecules with low aromaticity in BDOM underwent favorable association with Pb(II) and got re-adsorbed on RBC. With the increase in the Pb(II) concentration, the contribution of van der Waals interaction for adsorption of BDOM350-Pb complexes was improved, whereas adsorption mechanism in BDOM800-Pb complexes was more dependent on ligand exchange. This study provides mechanistic insights into the impact of BDOM on Pb(II) immobilization, which can provide valuable information for the long-term remediation of Pb-contaminated soils using BC.

Assessment of liver fibrosis by transient elastography in young children with chronic hepatitis B virus infection.

BACKGROUND: This study aimed to compare the diagnostic accuracy of transient elastography (TE) and biopsy for the detection of liver fibrosis in children with chronic hepatitis B (CHB). METHODS: This single-center prospective study included 157 CHB children aged 0-6 years. All patients underwent liver stiffness measurement (LSM) by TE and liver biopsy, separated by an interval of less than 1 week. RESULTS: The LSM, aspartate aminotransferase-platelet ratio index (APRI), and fibrosis-4 index (FIB-4) were positively correlated with activity grade and fibrosis stage in CHB children. The areas under the receiver operating characteristic curves (AUCs) of LSM for identifying significant (F ≥ 2) and advanced (F ≥ 3) fibrosis were 0.732 and 0.941, respectively. The cut-off values, specificity, and sensitivity for significant fibrosis were 5.6 kPa, 75.7%, and 67.4%, respectively; the corresponding values for advanced fibrosis were 6.9 kPa, 91.5%, and 81.3%, respectively. Compared to LSM, the overall diagnostic performances of APRI and FIB-4 for significant and advanced fibrosis were suboptimal, with low AUCs and sensitivity. Since LSM, platelet, and Log10 (hepatitis B surface antigen) were independent factors associated with the fibrosis stage (F < 2 and F ≥ 2), they were used to formulate the "LPS" index for the prediction of F ≥ 2. The AUC of LPS (for F ≥ 2) was higher than that of LSM (0.792 vs. 0.732, p < 0.05), and had an improved sensitivity (76.6% vs. 67.4%). CONCLUSIONS: TE is a promising technology for the diagnosis of advanced fibrosis in CHB children aged 0-6 years.

Identifying the Persistent Free Radicals (PFRs) Formed as Crucial Metastable Intermediates during Peroxymonosulfate (PMS) Activation by N-Doped Carbonaceous Materials.

A nonradical mechanism involved in peroxymonosulfate (PMS) activation in carbonaceous materials (CMs) is still controversial. In this study, we prepared N-doped CMs, including hollow carbon spheres (NHCSs) and carbon nanotubes (N-CNTs), to probe the crucial intermediates during PMS activation. The results suggested that the higher efficiency and lower activation energy (13.72 kJ mol-1) toward phenol (PN) degradation in an NHCS/PMS system than PMS alone (∼24.07 kJ mol-1) depended on a typical nonradical reaction. Persistent free radicals (PFRs) with a g factor of 2.0033-2.0045, formed as crucial metastable intermediates on NHCS or N-CNT in the presence of PMS, contribute largely to the organic degradation (∼73.4%). Solid evidence suggested that the formation of PFRs relied on the attack of surface-bonded •OH and SO4•- or peroxides in PMS, among which surface-bonded SO4•- was most thermodynamically favorable based on theoretical calculations. Electron holes within PFRs on NHCSs shifted the Fermi level to the positive energy with the valance band increasing from 1.18 to 1.98 eV, promoting the reactivity toward nucleophilic substances. The degradation intermediates of aromatic compounds (e.g., PN) and electron rearrangement triggered the evolution of PFRs from oxygen-centered to carbon-centered radicals. Moreover, due to the specific electron configuration, graphitic N on NHCS was critical for stabilizing the PFRs. This study provides insightful understanding of the fate of organic contaminants and the structure-activity relationship of reactivity of CMs toward PMS activation.

Transport and Retention of Free-Living Amoeba Spores in Porous Media: Effects of Operational Parameters and Extracellular Polymeric Substances.

Amoebas are protists that are widespread in water and soil environments. Some species are pathogenic, inducing potentially lethal effects on humans, making them a major threat to public health. Nonpathogenic amoebas are also of concern because they have the potential to carry a mini-microbiome of bacteria, either transiently or via more long-term stable transport. Due to their resistance to disinfection processes, the physical removal of amoeba by filtration is necessary to prevent their propagation throughout drinking water distribution networks and occurrence in tap water. In this study, a model amoeba species Dictyostelium discoideum was used to study the transport and retention behavior of amoeba spores in porous media. The key factors affecting the transport behavior of amoeba spores in fully saturated media were comprehensively evaluated, with experiments performed using a quartz crystal microbalance with dissipation monitoring (QCM-D) and parallel plate chamber system. The effects of ionic strength (IS) on the deposition of spores were found to be in contrast to the predicted Derjaguin-Landau-Verwey-Overbeek (DLVO) theory that more deposition is observed under lower-IS conditions. The presence of extracellular polymeric substances (EPS) was found to be the main contributor to deposition behavior. Overall, these results provide plausible evidence for the presence of amoeba in tap water. Furthermore, this is one of the first studies to examine the mechanisms affecting the fate of amoeba spores in porous media, providing a significant baseline for future research to minimize the safety risk presented by amoeba in drinking water systems.

Determination of Instinct Components of Biomass on the Generation of Persistent Free Radicals (PFRs) as Critical Redox Sites in Pyrogenic Chars for Persulfate Activation.

Persulfate (PS) activation on biochar (BC) is a promising technology for degrading the aqueous organic contaminants. However, the complexity of activation mechanisms and components in biomass that used to produce BC makes it difficult to predict the performance of PS activation. In this study, we employed eight sludges as the representative biomass that contained absolutely different organic or inorganic components. Results showed that the elemental composition, surface properties, and structures of the sludge-derived BCs (SBCs) clearly depended on the inherent components in the sludges. The intensities of persistent free radicals (PFRs) in the electron paramagnetic resonance (EPR) correlated positively with N-containing content of sludges as electron shuttle, but negatively with the metal content as electron acceptor. Linking with PFRs as crucial sites of triggering a radical reaction, a poly-parameter relationship of predicting PS activation for organic degradation using the sludge components was established (kobs,PN = 0.004 × Cprotein + 0.16 × CM-0.895 -0.118). However, for the PS activation on those SBCs without PFRs, this redox process only relied on the sorption or conductivity-related characteristics, not correlating with the content of intrinsic components in biomass but with pyrolysis temperatures. This study provided insightful information of predicting the remediation efficiency of PS activation on BCs and further understanding the fate of contaminants and stoichiometric efficiency of oxidants in a field application.

Biopsy-proven liver cirrhosis in young children: A 10-year cohort study.

Young children with liver cirrhosis have a significantly high risk of mortality. However, there are few studies regarding early childhood-onset cirrhosis. This study aims to explore the causes, clinical findings and prognosis of biopsy-proven cirrhosis in infants, toddlers and preschoolers. We enroled young children with biopsy-proven cirrhosis from January 2010. Till January 2020, the study has been going on for 10 years. A total of 139 cirrhotic children were enrolled, including 87 boys and 52 girls. The median age at initially histological diagnosis of cirrhosis was 2 years old (range: 1 month-6 years). Sixty-two patients reported yellowish discoloration of sclera and/or skin as an initial symptom. Ninety-three patients had definite aetiologies while 46 had indeterminate causes. Among the confirmed cases, 31 had hepatitis B virus (HBV) infection, accounting for 33.3%. Subsequently, glycogen storage disease was diagnosed in 16 cases and Wilson disease in 14 cases. In these patients with HBV infection, nine finally achieved hepatitis B surface antigen (HBsAg) loss (29.0%) after effective antiviral therapy during the follow-up. Logistic regression revealed that baseline alanine aminotransferase (odds ratio 1.008, p = 0.028) was the independent predictor of HBsAg loss. Furthermore, one patient who underwent second biopsies showed histological reverse. HBV infection is an important cause of paediatric cirrhosis in our study. The pathogenesis of HBV-related cirrhosis in early childhood deserves further studies.

Co-transport and retention of zwitterionic ciprofloxacin with nano-biochar in saturated porous media: Impact of oxidized aging.

While biochar (BC) is used for contaminant remediation (i.e. antibiotics) in the field, geochemical aging can alter its chemical structure, releasing nano-sized BC (NBC, sizes ranging from approximately 200 nm to 500 nm), and further influence the environmental behaviour of antibiotics affiliated with BC. In this study, we comprehensively examined the sorption behaviour of NBCs with and without aging toward ciprofloxacin (CIP), their aggregation performance, and transport behaviour in porous media. The results showed that aging improved the oxygen-containing groups within the NBCs and made their surfaces more negatively charged. The thermodynamic enhancements of specific interactions (i.e. π-π interaction or Coulombic force) with CIP resulted in the enhancement of slow sorption (from 60-64% to 40-58%) and a higher normalised sorption capacity (Qe). The aggregation of NBCs was affected by changes in individual specific interactions and interfacial forces between the NBCs before and after CIP sorption. Further, aging could enhance the transport of NBCs both in the absence and presence of CIP. In addition to the interaction with the quartz sand surface, the contributions of aggregation and chemical heterogeneity caused by rebalanced specific interactions with CIP, may explain the observed transport behaviours of the aged NBCs in porous media. Additionally, the presence of NBCs, regardless of aging, suppressed the transport of CIP. Thus, mechanisms such as increased sorption sites due to aggregation and competitive sorption between NBCs and CIP, rather than the contribution of co-transport from NBCs, might play an important role in determining the fate of CIP in the natural environment.

Coupling experiments with calculations to understand the thermodynamics evolution for the sorption of zwitterionic ciprofloxacin on oxidizing-aged pyrogenic chars in the aquatic system.

Oxidized aging due to the long-term exposure can significantly alter the sorption of pyrogenic chars (i.e., biochar, BC) towards antibiotics, which determined their fates in natural environments. In this study, the sorption of ciprofloxacin (CIP) on the oxidizing-aged BCs was studied linking the experimental thermodynamics and theoretical calculations. Results revealed that Q0 of CIP negatively correlated with their average site energies (Em), while pore-normalized Q0 on aged BCs were 2-6 folds higher than fresh BCs. From competitive sorption, it is proposed that the transformation of CIP± to CIP+ occurred and the π+-π electron donor-acceptor interaction and Coulombic attraction onto the aged BCs played a critical role. These two specific interactions with CIP were thermodynamically improved when aging degree increased and favored the free energies (ΔaG) of sorption by 2-5 kJ mol-1. Based on the identified relationship between experimental ΔOA-ΔG0 with Ea through DFT calculations, the contributions of the specific interactions to antibiotic sorption on aged BCs were quantified. This study provided an in-depth understanding of how the aging process affects the sorption of zwitterionic antibiotics on BCs and also possibilities to predict the fate of antibiotics in the presence of BCs over a long-term period.