Evaluation of the EMBOPIPE flow diverter device: in vivo and in vitro experiments.

BACKGROUND: Although flow diverter device (FDD) has brought revolutionized advances in endovascular treatment of intracranial aneurysms, it also presents considerable drawbacks as well, as the innovation for novel device has never stopped. This preclinical research aims to evaluate the safety and efficacy of a newly developed FDD, the EMBOPIPE, through in vivo and in vitro experiments. METHODS: Aneurysms were induced in 20 New Zealand white rabbits which were randomized to three follow-up groups according to the time elapsed after EMBOPIPE implantation (28, 90, and 180 days). Additional EMBOPIPEs were implanted in the abdominal aorta to cover the renal artery in nine rabbits. Angiography was performed immediately after device placement in all groups. Aneurysm occlusion, patency of renal arteries, and pathological outcomes were assessed. For the in vitro experiments, we measured the thrombogenic potential of EMBOPIPEs (n = 5) compared with bare stents (n = 5) using the Chandler loop model. Evaluation indicators were the platelet counts, macroscopic observations and scanning electron microscopy. RESULTS: EMBOPIPEs were successfully deployed in 19 of 20 rabbit aneurysms (95.0%). The rates of complete or near-complete aneurysm occlusion were 73.3%, 83.3%, and 100% in the 28-, 90-, and 180-day groups, respectively. All renal arteries covered by EMBOPIPEs remained patent, and the mean difference in renal artery diameter before and after the device placement in the three groups was 0.07 mm, 0.10 mm, and 0.10 mm, respectively (p = 0.77). Renal pathology was normal in all cases. The pathological findings of the aneurysms were as follows: thickened and adequate neointimal coverage at the aneurysm neck, minimal inflammatory response, near-complete smooth muscle cell layer, and endothelialization along the device. In vitro experiments showed that the platelet counts were significantly higher in EMBOPIPE blood samples than in bare stent samples and that platelet adhesion to the device was lower in the EMBOPIPE stent struts compared with bare stent struts through macroscopic observations and scanning electron microscopy. CONCLUSIONS: The EMBOPIPE can achieve high rates of aneurysm occlusion while maintaining excellent branch artery patency. It exhibited wonderful pathological results. This novel device with phosphorylcholine surface modification could reduce platelet thrombus attached to the stent struts.

Optimizing the structure of food production in China to improve the sustainability of water resources.

The conflict between the growing demand for food and limited water resources is intensifying. To further elucidate the relationship between food and water, we construct a water footprint life cycle assessment framework for food products and propose a modified algorithm for measuring a food's water footprint to assess the virtual water transfer between grain crops and animal products. To address the mismatch between regional water resources and food production, we propose a novel optimization model for food production structure, with both reducing water use and maintaining food security as its objectives. Using 2020 as an example, the analysis proposes an adjusted food production structure for China at national, regional, and provincial scales. The results show that 24.9 % of water consumed by grain crops is transferred to animal products through feed grain. The total water footprint of food production in China is 820.8 billion m3, with the blue water footprint accounting for 32.9 % of that total. The blue water footprint for food production in northern China is 161.8 billion m3, which is much larger than 108.2 billion m3 in southern China. Water scarcity is also greater in northern regions, which produce the majority of grain and animal products. Our optimization shows that a reasonable food production structure can balance water resources and food security by remarkably reducing China's total blue water footprint and increasing food production in the south while reducing production in certain northern provinces to ensure sustainable regional development.

Use of Tirofiban to Prevent Ischemic Events in Patients with CYP2C19 Loss-of-Function Alleles during Flow Diversion of Intracranial Aneurysm: A Multicenter Cohort Study.

To analyze the effect of tirofiban on ischemic events in CYP2C19 loss-of-function (LOF) allele carriers during pipeline embolization device (PED) implantation. Demographic information, imaging data, ischemic complications, CYP2C19 genotyping, and platelet function test results were collected from patients with PED-treated intracranial aneurysms at three centers. Multivariate logistic regression was used to analyze risk factors for ischemic events. Patients were grouped according to LOF alleles and antiplatelet drugs, the baseline information of LOF allele carriers and non-carriers were compared, and the efficacy of tirofiban was analyzed by comparing the incidence of ischemic events in each group. In total, 278 patients were included in the study, 24 of whom had an ischemic event. 157 (56.5%) patients carried the LOF allele and were more likely to develop resistance to clopidogrel (P < 0.001) and hypertension (P = 0.010). Multivariate logistic regression analysis revealed that the independent risk factors for ischemic events were age of > 55 years (OR = 3.308, P = 0.028), LOF alleles (OR = 3.960, P = 0.036), and clopidogrel nonresponsiveness (OR = 3.301, P = 0.014). For LOF allele carriers, prophylactic use of tirofiban after PED implantation helped to reduce ischemic events (4.3% vs. 16.4%, P = 0.039). This study supports CYP2C19 genotyping before flow diversion because LOF alleles increase the risk of ischemic events. Prophylactic use of tirofiban may help reduce ischemic events in LOF allele carriers.

Roles of light transmission aggregometry and CYP2C19 genotype in predicting ischaemic complications during interventional therapy for intracranial aneurysms.

BACKGROUND AND PURPOSE: Light transmission aggregometry (LTA) and CYP2C19 genotype analysis are commonly used to evaluate the antiplatelet effects of clopidogrel during the interventional treatment of intracranial aneurysms. The aim of this study was to determine which test can predict ischaemic events during these treatments. METHODS: Patient demographic information, imaging data, laboratory data and ischaemic complications were recorded. LTA and CYP2C19 genotype results were compared, and multiple linear regression was performed to examine factors related to platelet reactivity. Multivariate regression analysis was performed to determine whether LTA and CYP2C19 could predict ischaemic complications and to identify other clinical risk factors. Receiver operating characteristic curve analysis was conducted to calculate the cut-off value for predicting ischaemic complications. A subgroup analysis was also performed for different CYP2C19 genotype metabolisers, as well as for patients with flow diverters and traditional stents. RESULTS: A total of 379 patients were included, of which 22 developed ischaemic events. Maximum platelet aggregation induced by ADP (ADP-MPA) could predict ischaemic events (p<0.001; area under the curve, 0.752 (95% CI 0.663 to 0.842)), and its cut-off value was 41.5%. ADP-MPA (p=0.001) and hypertension duration >10 years (p=0.022) were independent risk factors for ischaemic events, while the CYP2C19 genotype was not associated with ischaemic events. In the subgroup analysis, ADP-MPA could predict ischaemic events in fast metabolisers (p=0.004) and intermediate metabolisers (p=0.003). The cut-off value for ischaemic events was lower in patients with flow diverters (ADP-MPA=36.4%) than in patients with traditional stents (ADP-MPA=42.9%). CONCLUSIONS: ADP-MPA can predict ischaemic complications during endovascular treatment of intracranial aneurysms. Patients with flow diverters require stronger antiplatelet medication than patients with traditional stents.

A copula-based security risk evaluation and probability calculation for water-energy-food nexus.

Water-energy-food (WEF) are essential for human survival and development and they interact complexly. The research on Water-energy-food-coupling security risk (WEF-CSR) is crucial to promoting multi resource collaborative management. In this study, a comprehensive index system was constructed using three dimensions: reliability (Ra), coordination (C), and resilience (Rs). We selected the middle and upper reaches of the Yellow River Basin in China to study WEF-CSR conflicts, coupled with the Copula method of risk probability calculation. The results showed that: 1) from 2005 to 2021, the WEF-CSR index in the study area showed a progressively increasing trend, with a mean value of 0.49 in a critical safety state and lower values in the upper reaches of Ningxia and Gansu provinces. 2) The Ra-indexes in the criterion layer is not clearly affected by resource endowment conditions between annual periods; C-indexes are growing rapidly, with their annual growth rate four times as fast as the Ra-indexes, and Rs-indexes growing at about 2.7 times faster. 3) "Energy consumption per capita, carbon emissions, and water-related indicators" are the six main limiting factors, with a combined impediment degree of over 35 %, water resources are major constraint. 4) The Ra-C, C-Rs, and Ra-C-Rs indexes are well fitted by Clayton copula, and the C-Rs indexes are well fitted by Frank copula. The three-dimensional joint distribution risk probability, Ra-C-Rs (Ra ≤ 0.4, C ≤ 0.4, Rs ≤ 0.4) is 0.29. When the Ra or C indexes increases, the probability of unsafe Rs rises to 40 %-50 %, meaning future development must emphasize synergy, especially in Rs. In future, the five provinces in the middle and upper reaches of the Yellow River should focus on reducing energy pressure, improving water shortage conditions, and enhancing the overall resilience to avoid single-factor adverse conditions affecting WEF's coordinated and sustainable development.

Energy-water security challenge: Impact of energy production on water sustainable developments in Northwest China in 2017 and 2030.

Energy consumption has gradually become an important factor affecting the sustainable use of regional water resources, especially in areas with abundant energy but scarce water. In this research, the water consumption for energy production in arid Northwest China (NWC) in 2017 and 2030 was evaluated, and the virtual water (VW) transfer embodied in the energy trade was also calculated based on a large amount of data collected from multiple sources. The results showed that the energy-related water consumption in NWC in 2017 was 2.6 billion m3, accounting for approximately 3.4% of the total regional water consumption and 61.8% of the total regional industrial water consumption. This value is projected to reach 8.6 billion m3 in 2030 under a normal water consumption scenario (BAU scenario), and 5.4 and 3.6 billion m3 under a regular water-saving scenario (RWS scenario) and enhanced water-saving scenario (EWS scenario), respectively. In 2017, except for Qinghai and Gansu, the other province in the study area was a VW exporter and the total VW output volume was 710.3 million m3, accounting for 26.9% of the total water consumption for energy production, this ratio will reach approximately 60% in 2030 due to the expansion of energy industry. In addition, based on our research, the available water in four provinces cannot meet the future energy requirements under EWS scenario owing to the water shortage, however, different energy development strategies need to be selected faced with the different types of water shortage condition. This study also proposed some countermeasures to ensure the coordinated development of regional water and energy.

Water stress assessment integrated with virtual water trade and physical transfer water: A case study of Beijing, China.

With the increase in population and economic development, urban water demand has increased significantly over the past decades, and physical transfer water (i.e., freshwater transported from water source regions to water intake regions through channels or pipelines) and virtual water (i.e., freshwater used in the production of goods and services along their supply chains, abbreviated as VW) have gradually become viable water sources for many cities to relieve water stress. This study used Beijing as the research object and systematically analyzed the impact of physical transfer water, VW, and local water on urban water stress from 2000 to 2016. The results show that VW inflow related to food trade has increased from 3.55 billion m3 in 2000 to 16.76 billion m3 in 2016, and that energy's VW inflow increased from 52.76 million m3 to 137.47 million m3 over the same period. Before 2011, Beijing's water demand was largely met by local water resources; however, after 2011, external water resources (including physical transfer water and VW) accounted for majority of the city's water demand, and VW's contribution increased from 47% in 2011 to 53% in 2016. Although Beijing has significantly reduced local water use in favor of external sources, its water stress index in 2016 remained considerable, far exceeding the upper limit of available water. This study also proposes some measures to ensure Beijing's water security based on the sustainability of external water supply.

Energy Reduction Effect of the South-to-North Water Diversion Project in China.

The North China Plain, with a population of approximately 150 million, is facing severe water scarcity. The over-exploitation of groundwater in the region, with accumulation amounts reaching more than 150 billion m3, causes a series of hydrological and geological problems together with the consumption of a significant amount of energy. Here, we highlight the energy and greenhouse gas-related environmental co-benefits of the South-to-North Water Diversion Project (SNWDP). Moreover, we evaluate the energy-saving effect of SNWDP on groundwater exploitation based on the groundwater-exploitation reduction program implemented by the Chinese government. Our results show that the transferred water will replace about 2.97 billion m3 of exploited groundwater in the water reception area by 2020 and hence reduce energy consumption by 931 million kWh. Further, by 2030, 6.44 billion m3 of groundwater, which accounts for 27% of the current groundwater withdrawal, will save approximately 7% of Beijing's current thermal power generation output.

WebGIVI: a web-based gene enrichment analysis and visualization tool.

BACKGROUND: A major challenge of high throughput transcriptome studies is presenting the data to researchers in an interpretable format. In many cases, the outputs of such studies are gene lists which are then examined for enriched biological concepts. One approach to help the researcher interpret large gene datasets is to associate genes and informative terms (iTerm) that are obtained from the biomedical literature using the eGIFT text-mining system. However, examining large lists of iTerm and gene pairs is a daunting task. RESULTS: We have developed WebGIVI, an interactive web-based visualization tool ( http://raven.anr.udel.edu/webgivi/ ) to explore gene:iTerm pairs. WebGIVI was built via Cytoscape and Data Driven Document JavaScript libraries and can be used to relate genes to iTerms and then visualize gene and iTerm pairs. WebGIVI can accept a gene list that is used to retrieve the gene symbols and corresponding iTerm list. This list can be submitted to visualize the gene iTerm pairs using two distinct methods: a Concept Map or a Cytoscape Network Map. In addition, WebGIVI also supports uploading and visualization of any two-column tab separated data. CONCLUSIONS: WebGIVI provides an interactive and integrated network graph of gene and iTerms that allows filtering, sorting, and grouping, which can aid biologists in developing hypothesis based on the input gene lists. In addition, WebGIVI can visualize hundreds of nodes and generate a high-resolution image that is important for most of research publications. The source code can be freely downloaded at https://github.com/sunliang3361/WebGIVI . The WebGIVI tutorial is available at http://raven.anr.udel.edu/webgivi/tutorial.php .

PathRings: a web-based tool for exploration of ortholog and expression data in biological pathways.

BACKGROUND: High-throughput methods are generating biological data on a vast scale. In many instances, genomic, transcriptomic, and proteomic data must be interpreted in the context of signaling and metabolic pathways to yield testable hypotheses. Since humans can interpret visual information rapidly, a means for interactive visual exploration that lets biologists interpret such data in a comprehensive and exploratory manner would be invaluable. However, humans have limited memory capacity. Current visualization tools have limited viewing and manipulation capabilities to address complex data analysis problems, and visual exploratory tools are needed to reduce the high mental workload imposed on biologists. RESULTS: We present PathRings, a new interactive web-based, scalable biological pathway visualization tool for biologists to explore and interpret biological pathways. PathRings integrates metabolic and signaling pathways from Reactome in a single compound graph visualization, and uses color to highlight genes and pathways affected by input data. Pathways are available for multiple species and analysis of user-defined species or input is also possible. PathRings permits an overview of the impact of gene expression data on all pathways to facilitate visual pattern finding. Detailed pathways information can be opened in new visualizations while maintaining the overview, that form a visual exploration provenance. A dynamic multi-view bubbles interface is designed to support biologists' analytical tasks by letting users construct incremental views that further reflect biologists' analytical process. This approach decomposes complex tasks into simpler ones and automates multi-view management. CONCLUSIONS: PathRings has been designed to accommodate interactive visual analysis of experimental data in the context of pathways defined by Reactome. Our new approach to interface design can effectively support comparative tasks over substantially larger collection than existing tools. The dynamic interaction among multi-view dataset visualization improves the data exploration. PathRings is available free at http://raven.anr.udel.edu/~sunliang/PathRings and the source code is hosted on Github: https://github.com/ivcl/PathRings .