Microbial mechanisms involved in negative effects of amoxicillin and copper on humification during composting of dairy cattle manure.

Livestock manure often contains various pollutants. The aim of this study was to investigate how adding amoxicillin (AMX), Cu, and both AMX and Cu (ACu) affected humification during composting and the microbial mechanisms involved. The cellulose degradation rates were 16.96%, 10.86%, and 9.01% lower, the humic acid contents were 18.71%, 12.89%, and 16.78% lower, and the humification degrees were 24.72%, 24.16%, and 15.73% lower for the AMX, Cu, and ACu treatments, respectively, than the control. Adding AMX and Cu separately or together inhibited humic acid formation and decreased the degree of humification, but the degree of humification was decreased less by ACu than by AMX or Cu separately. The ACu treatment decreased the number of core bacteria involved in humic acid formation and decreased carbohydrate and amino acid metabolism during the maturing period, and thereby delayed humic acid formation and humification. The results support composting manure containing AMX and Cu.

Responses of greenhouse gas emissions to aeration coupled with functional membrane during industrial-scale composting of dairy manure: Insights into bacterial community composition and function.

Greenhouse gas (GHG) emissions from manure management processes deserve more attention. Using three industrial-scale experiments, this study comprehensively evaluated the effects of different aeration coupled with semi-permeable membrane-covered strategies on the structure and function of bacterial communities and their impact on GHG emissions during dairy manure aerobic composting. The succession of the bacterial communities tended to be consistent for similar aeration strategies. Ruminiclostridium and norank_f__MBA03 were significantly positively correlated with the methane emission rate, and forced aeration coupled with semi-permeable membrane-covered decreased GHG emissions by inhibiting these taxa. Metabolism was the most active function of the bacterial communities, and its relative abundance accounted for 75.69%-80.23%. The combined process also enhanced carbohydrate metabolism and amino acid metabolism. Therefore, forced aeration coupled with semi-permeable membrane-covered represented a novel strategy for reducing global warming potential by regulating the structure and function of the bacterial communities during aerobic composting of dairy manure.

Nitrogen evolution during membrane-covered aerobic composting: Interconversion between nitrogen forms and migration pathways.

Aerobic composting is a promising technology for converting manure into organic fertilizer with low capital investment and easy operation. However, the large nitrogen losses in conventional aerobic composting impede its development. Interconversion of nitrogen species was studied during membrane-covered aerobic composting (MCAC) and conventional aerobic composting, and solid-, liquid-, and gas-phase nitrogen migration pathways were identified by performing nitrogen balance measurements. During the thermophilic phase, nitrogenous organic matter degradation and therefore NH3 production were faster during MCAC than uncovered composting. However, the water films inside and outside the membrane decreased NH3 release by 13.92%-22.91%. The micro-positive pressure environment during MCAC decreased N2O production and emission by 20.35%-27.01%. Less leachate was produced and therefore less nitrogen and other pollutants were released during MCAC than uncovered composting. The nitrogen succession patterns during MCAC and uncovered composting were different and NH4+ storage in organic nitrogen fractions was better facilitated during MCAC than uncovered composting. Overall, MCAC decreased total nitrogen losses by 33.24%-50.07% and effectively decreased environmental pollution and increased the nitrogen content of the produced compost.