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Artículo en Ruso | MEDLINE | ID: mdl-25051696

RESUMEN

AIM: VNTR-typing of Vibrio cholerae strains isolated in the territory of Russian Federation in 2012. MATERIALS AND METHODS: 71 Vibrio cholerae O3 and 3 V cholerae O1/O139 strains were used in the study. Genotyping was performed by using PCR for 5 VNTR-loci. RESULTS: Multilocus VNTR-typing allowed to group the strains into 31 VNTR-genotypes. Genotypes were divided among 10 discrete clusters by results of a cluster analysis. The presence of tcpA gene is clearly linked with the presence of VcB locus. Each geographic region was characterized by their own VNTR-genotypes. CONCLUSION: In the course of the carried out VNTR-genotyping of V. cholerae isolated in 2012, 2 types of vibrio population formation were detected. A geographic attachment to specific regions was characteristic for most of the genotypes.


Asunto(s)
Proteínas Fimbrias/genética , Repeticiones de Minisatélite , Filogenia , Vibrio cholerae/genética , Cólera/epidemiología , Cólera/microbiología , Medios de Cultivo , Proteínas Fimbrias/clasificación , Expresión Génica , Sitios Genéticos , Genotipo , Humanos , Tipificación de Secuencias Multilocus/métodos , Filogeografía , Reacción en Cadena de la Polimerasa , Federación de Rusia/epidemiología , Vibrio cholerae/clasificación , Vibrio cholerae/aislamiento & purificación
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