CT scan structured report for the study of abdominal wall defects: a fast, easy and practical tool at the service of both surgeons and radiologist.

PURPOSE: The advantages offered by structured reporting have already been highlighted in the literature. However, there is still no evidence on the validity of this reporting method for the study of abdominal wall defects. This study aims to show the experience of the Trentino Hernia Team (THT) multidisciplinary group in the development and use of a structured CT scan report for the study of abdominal wall defects. METHODS: A regional multidisciplinary team (THT group) used a Delphi method to identify and select the most important CT scan parameters needed to describe and stage abdominal wall defects for correct preoperative planning. Based on the selected parameters, a CT scan structured report was worked out and collectively accepted. The first 20 structured reports obtained were individually tested for compilation speed and homogeneity of the data reported by five distinct radiologists. The reports were then evaluated by five different surgeons to test the simplicity of interpretation. RESULTS: We produced a model of a structured report for the study of the abdominal wall defects and tested it in our hospital network on the first 20 reports. The average completion time was 18 min (range 12-25). There was no heterogeneity among the reported data. The reports were analysed by five distinct surgeons to evaluate completeness and simplicity of interpretation. Each surgeon used a Likert scale from 0 to 5 to evaluate each report, producing average scores of 4.8 and 4.1 for completeness and comprehensibility respectively, with a mean combined total score of 8.9 out of 10. CONCLUSIONS: Our structured report represents a fundamental tool capable of providing the surgeon with all the measurements of the parameters necessary for correct preoperative planning. At the same time, it is of crucial help for the radiologists representing an easy and fast way to report all the needed parameters using the same standards.

Prospective observational study of abdominal wall reconstruction with THT technique in primary midline defects with diastasis recti: clinical and functional outcomes in 110 consecutive patients.

INTRODUCTION: Surgical treatment of diastasis recti is still a matter of debate. Open approaches such as abdominoplasty, which offer the possibility to combine reparation of the diastasis with abdominal cosmetic surgery, are challenged by the emerging less-invasive laparoscopic or robotic techniques that offer shorter recovery for patients. However, evidence in favour of one of the two approaches concerning both short- and long-term complications and functional results is still lacking. In this paper, we analysed clinical and functional results of a new endo-laparoscopic technique for midline reconstruction (THT technique) in patients with primary abdominal wall defects associated with diastasis recti. METHODS: Prospective observational study on 110 consecutive patients was submitted to endo-laparoscopic reconstruction of the abdominal wall with linear staplers. Morbidity and relapse rates with clinical and radiological follow-up were recorded at 1, 6, 12, and 24 months after the operation. Data regarding the impact of surgery on patients' quality of life (EuraHSQol) on chronic low back pain (Oswestry Disability Index, ODI) and urinary stress incontinence (Incontinence Severity Index, ISI) were gathered. RESULTS: After a mean follow-up of 14 months, the morbidity rate was 9.1% and no recurrences were recorded. 6-month follow-up ultrasound showed a rectus muscles mean distance of 6.7 mm; EuraHSQol, ODI, and ISI scores significantly improved in 93%, 77%, and 63% of the cases, respectively. CONCLUSIONS: The THT technique proved to be a feasible, safe, and effective alternative for corrective surgery of primary midline hernias associated with diastasis recti. Short- and mid-term results are encouraging but need to be confirmed by further studies with longer follow-up. The achieved midline reconstruction offers a significant improvement of patients' perceived quality of life through reduction of abdominal wall pain, bulging, low back pain, and urinary stress incontinence.

Micro-CT assessment of the shaping ability of four root canal instrumentation systems in oval-shaped canals.

AIM: To compare the shaping ability of four root canal instrumentation systems in oval-shaped canals using micro-computed tomographic analysis. METHODOLOGY: Forty anatomically matched mandibular incisors were scanned and assigned to four groups (n = 10), according to the canal preparation protocol: BioRace, Reciproc, Self-Adjusting File (SAF) and TRUShape systems. After canal instrumentation, the specimens were rescanned, and the registered pre- and postoperative datasets were examined to evaluate the percentages of accumulated hard-tissue debris, untouched canal walls and dentine removed. Kruskal-Wallis and Mann-Whitney U-tests with Bonferroni correction were used to compare the variables in the groups (α = 5%). RESULTS: The preparation techniques did not affect the percentage of accumulated hard-tissue debris (P = 0.126). The percentage of untouched canal areas was significantly higher for BioRace (32.38%)compared to Reciproc (18.95%) and SAF (16.08%) systems (P < 0.05). Reciproc removed significantly more dentine (4.18%) than BioRace (2.21%) and SAF (2.56%) (P < 0.05). The TRUShape system had intermediate results for both untouched canal walls (19.20%) and dentine removed (3.77%), with no significant difference compared to BioRace, Reciproc and SAF systems. CONCLUSIONS: The preparation techniques resulted in the same level of accumulated hard-tissue debris. Compared to the other tested systems, BioRace was associated with more untouched canal walls and Reciproc produced the greatest amount of removed dentine. Although it touched more of the root canal walls, the SAF system removed less dentine, whereas TRUShape had intermediate results for these same parameters. None of the systems tested were able to provide optimal shaping ability in oval-shaped canals.

CHIVA strategy in chronic venous disease treatment: instructions for users.

Along the years, scientific clinical data have been collected concerning the possible saphenous flow restoration without any ablation and according with the CHIVA strategy. Moreover, in 2013 a Cochrane review highlighted the smaller recurrence risk following a CHIVA strategy rather than a saphenous stripping. Nevertheless, the saphenous sparing strategy surely remains a not-so-worldwide-spread and accepted therapeutic option, also because considered not so immediate and easy to perform. Aim of this paper is to provide an easily accessible guide to an everyday use of a saphenous sparing strategy for chronic venous disease, highlighting how even apparently too complicated reflux patterns classifications can be fastly and successfully managed and exploited for a hemodynamic correction.

Efficacy of reciprocating and rotary techniques for removing filling material during root canal retreatment.

AIM: To compare the efficacy of reciprocating and rotary techniques with that of hand files for removing gutta-percha and sealer from root canals. METHODOLOGY: The root canals of fifty-four human extracted maxillary central incisors were cleaned and shaped using a crown-down technique to a size 40 and filled with gutta-percha and a zinc oxide-eugenol-based sealer using a lateral compaction technique. Teeth were divided into three groups according to the technique used for removing the root filling material: group I - Gates-Glidden burs and stainless steel hand files up to size 50; group II - rotary technique with NiTi Mtwo R files and additional Mtwo files to size 50, 0.04 taper; group III - reciprocating technique with the Reciproc instrument R50, size 50, 0.05 taper. Chloroform was used as a solvent in all groups. Teeth were then split longitudinally and photographed under 8× magnification. The images were transferred to a computer, and the total canal space and remaining filling material were quantified. The ratio of remaining filling material to root canal periphery was computed with the aid of Image Tool 3.0 software. The mean percentages of remaining filling material and time required to remove it were compared using the Kruskal-Wallis and Mann-Whitney tests (P < 0.05). RESULTS: The mean percentage of remaining filling material was significantly higher (P < 0.05) in group II, with Mtwo rotary files (12.17%), than in group I, with the hand file technique (7.19%), and group III, with Reciproc instruments (4.57%), which were statistically similar (P > 0.05). The time required to remove filling material was significantly shorter (P < 0.05) in group III (194 s), followed by group II (365 s) and group I (725 s) (P < 0.05). CONCLUSION: Remaining endodontic filling material was observed on the canal walls of all teeth regardless of the technique used. Hand files combined with Gates-Glidden burs (group I) and the reciprocating technique (group III) removed more filling material from the canal walls than the Mtwo R files. The reciprocating technique was the most rapid method for removing gutta-percha and sealer, followed by the rotary technique and the hand file technique.

Panbacterial real-time PCR to evaluate bacterial burden in chronic wounds treated with Cutimed™ Sorbact™.

The impact of polymicrobial bacterial infection on chronic wounds has been studied extensively, but standard bacteriological analysis is not always sensitive enough. Molecular approaches represent a promising alternative to the standard bacteriological analysis. This work aimed to assess the usefulness of a panbacterial quantitative real-time PCR reaction to quantitate the total bacterial load in chronic wounds treated with Cutimed™ Sorbact™, a novel therapeutic approach based on hydrophobic binding of bacteria to a membrane. The results obtained by panbacterial real-time PCR on conserved sequences of the bacterial 16S gene show that the bacterial burden significantly decreased in 10 out of 15 healing chronic wounds, and did not change in 5 out of 5 non-healing chronic wounds. On the contrary, classical culture for S. aureus and P. aeruginosa, and real-time PCR for Bacteroides and Fusobacterium did not show any correlation with the clinical outcome. Our study also shows that quantification of chronic wounds by panbacterial real-time PCR is to be performed on biopsies and not on swabs. These results show that panbacterial real-time PCR is a promising and quick method of determining the total bacterial load in chronic wounds, and suggest that it might be an important biomarker for the prognosis of chronic wounds under treatment.

The reproducibility of colour Doppler in chronic cerebrospinal venous insufficiency associated with multiple sclerosis.

AIM: Chronic cerebrospinal venous insufficiency (CCSVI) is a syndrome described in multiple sclerosis (MS) patients, characterized by stenosis of the main extracranial veins with hampered cerebral venous outflow. In the original description echo-colour Doppler demonstrated to be an ideal non invasive tool for screening CCSVI patients, but the reproducibility was not assessed. Aim of this study is to assess the variability coefficient between trained and in not trained echo-colour Doppler operators. METHODS: Thirty-six (36) subjects, matched for age and gender, were subset in 3 groups (group A, 12 healthy controls, HC; group B, 12 multiple sclerosis patients, MS; group C, 12 patients with other neurological disease, OND) underwent echo-colour Doppler screening for CCSVI according to an original protocol previously described. The inter observer variability rate was assessed by comparing respectively trained vs not trained operators, and trained vs trained operators, by using the same echo-colour Doppler equipment. In addition, by scanning 15 subjects after one month from the first session, intra observer coefficient was also assessed in trained operator. RESULTS: The inter observer variability rate between trained and not trained echo-colour Doppler operators, were not completely satisfactory (K coefficient 0.47 95% CI 0.27-0.68). To the contrary the inter observer agreement between trained operators was much more reliable (K coefficient 0.80 95% CI 0.59-1.01). Finally, the intra observer variability rate in trained operators was 0.93, (95% CI 0.80-1.06) confirming a highly satisfactory agreement. CONCLUSION: Echo-colour Doppler is a powerful, non-invasive and reproducible tool for screening CCSVI-MS but it needs special training.

Identification of GM3 as a marker of therapy-resistant periradicular lesions.

The purpose of this study was to analyze the profile of glycosphingolipids (GSLs) in periradicular lesions refractory to endodontic treatment. Sixteen periapical lesions were removed surgically from patients (experimental group) and compared with 10 samples of periodontal ligament removed from extracted intact third molars (control group). After the GSLs extraction and purification procedures were performed the neutral and acidic GSL fractions were analyzed by high-performance thin-layer chromatography and quantified by densitometry. Data reported herein show that: (i) tissues in the experimental group presented about twice as much GSLs as the control group; (ii) lesion tissues express lactoneotetraosylceramide, and lactofucopentaosyl (IV) ceramide, whereas these neutral GSLs are absent in normal tissues; and (iii) normal tissues express GT1b, whereas lesions cells do not express this ganglioside. In contrast lesion tissues express GM3, which is conspicuously absent in normal tissues.

Orifice locating with a microscope.

The purpose of this study was to determine whether the use of the dental operating microscope (DOM) could increase the number of root canal orifices located in mandibular molars. Ninety-three first and 111 second extracted mandibular molars were used. With the naked eye, all access cavities were prepared and the number of canals in each root was recorded. Using a DOM with x8-x13 magnification, all teeth had the access cavity preparations again examined. With the naked eye, a total of 641 canals were seen in all teeth. After the DOM examination, 50 more canals could be visualized, representing a 7.8% increase in the total number of located canals. From these canals, 35 were located in the first molars and 15 in the second molars. The use of the DOM increases the number of root canal orifices located.

Prognosis in periradicular surgery: a clinical prospective study.

AIM: The purpose of this prospective study was to evaluate the prognosis of periradicular surgery using well-defined case selection and a rigorous surgical protocol. METHODOLOGY: Teeth to be treated surgically demonstrated a periradicular lesion of strictly endodontic origin with or without clinical signs and symptoms of inflammation. A total of 114 teeth were treated. Following the reflection of a full mucoperiosteal tissue flap, residual soft tissues were curetted, root ends were resected with a fine high-speed diamond bur, root-end cavities were prepared ultrasonically with diamond tips, and IRM root-end fillings were placed. Cases were followed clinically and radiographically for a period ranging from 1 to 4 years. RESULTS: The results of this study showed 91.2% success out of a total of 102 teeth available for follow-up, based on accepted parameters of evaluation. Cases were considered successful if there were no clinical signs or symptoms present and there was radiographic evidence of complete or incomplete healing (scar tissue). Factors related to case selection, parameters of healing and surgical technique are discussed in relation to the success rate identified in this prospective study. CONCLUSIONS: Adherence to a strict endodontic surgical protocol and the use of contemporary techniques and materials will result in a predictably successful outcome in a wide range of teeth.

Ultrasonic root-end preparation with smooth and diamond-coated tips.

The purpose of this study was to compare the effects of smooth and diamond-coated ultrasonic retrotips on the external and internal surfaces of root-end preparations with the aid of a scanning electron microscope (SEM). Forty-four mesial roots of human mandibular molars were selected. The canals were cleaned, shaped and obturated using gutta-percha and sealer. The apical portions were resected at a 45 degrees-angle bevel exposing both mesial canals and the isthmus area. The roots were then divided into two groups according to the type of root-end preparation: Group A--performed with smooth retrotips (S) and Group B--performed with diamond-coated retrotips (DC). The specimens were coded and prepared for SEM evaluation. Observations of the external surface preparation showed that the S and DC retrotips produced very well-centered cavities involving both canals and isthmus area with minimal deviations and no perforative defects. When the internal surface of the root-end preparations was evaluated, it was evident that the use of S retrotips resulted in clean canal walls with little superficial debris and smear layer. Internal canal surfaces done with DC retrotips were irregular showing patent grooves, in contrast with the more uniform, regular and smoother surfaces when S retrotips were employed.

Sealing ability of composite resin placed over calcium hydroxide and calcium sulphate plugs in the repair of furcation perforations in mandibular molars: a study in vitro.

The purpose of this study was to evaluate in vitro the sealing ability of various materials in the repair of furcation perforations in mandibular molars by measuring coronal microleakage with Indian ink. Ninety extracted mandibular molars were embedded individually into a plaster of Paris block, with the roots surrounded by a simulated periodontal ligament of silicone. Subsequently, a standard coronal access opening was prepared, the root canal orifices were located and a perforation was made with a size 012 round bur in a water-cooled high-speed handpiece directly into the centre of the floor of the pulp chamber. The perforations were repaired with amalgam, composite resin, calcium sulphate under composite resin and calcium hydroxide under composite resin. The teeth were coated with two layers of nail polish, leaving the access opening area uncovered, and immersed in Indian ink for 4 days at 37 degrees C. The teeth were sectioned longitudinally and dye penetration measured from the coronal level of the repair material to the apical end of the perforation. All experimental groups revealed dye penetration in varying degrees, but there was no significant difference amongst them (Kruskal-Wallis test P < 0.05). Calcium sulphate and calcium hydroxide prevented overextrusion of composite resin when used under this repair material.

Instrument deterioration with usage: nickel-titanium versus stainless steel.

Nickel-titanium instruments purportedly resist deformation and loss of sharpness better than do stainless steel instruments but may be more susceptible to breakage. The processes of wear and breakage of nickel-titanium and stainless steel instruments were examined. Sixty files of five types (12 each) and three manufacturers were used. All were used repeatedly in curved canals until failure or for a maximum of 22 minutes. Each instrument was examined with scanning electron microscopy both new (control) and at spaced intervals for evidence of wear and fatigue. All new instruments were of good quality. Stainless steel instrument tended to wear the most rapidly, and next were nickel-titanium rotary instruments; the most resistant to wear were nickel-titanium hand instruments. There were few instrument separations. In general, nickel-titanium (particularly hand) instruments resisted deterioration better than did stainless steel. Nickel-titanium rotary instruments (2 of 12) had the most breakage.

Bacterial penetration through temporary restorative materials in root-canal-treated teeth in vitro.

Seventy extracted single-rooted mandibular premolars were used in this study to determine the length of time needed for bacteria present in natural human saliva to penetrate through three commonly used temporary restorative materials and through the entire root canal system obturated with the lateral condensation technique. Five intact teeth with no decay were used in the negative control group and five teeth with extensive decay in communication with the canal, in the positive control group. The 60 teeth used in the experimental group were divided into three groups. In Group 1, the access cavities of 20 teeth were temporarily restored with gutta percha, in Group 2 with IRM and in Group 3 with Cavit-G. All 70 teeth were mounted individually where the crown was placed in contact with human saliva. All five teeth in the positive control group caused broth turbidity within 24 h. In contrast, broth in the negative control group remained clear throughout the entire experimental period. In the experimental group, the average time for broth contamination of access cavities closed with gutta percha, IRM and Cavit-G was 7.85, 12.95 and 9.80 days, respectively. The Kruskal-Wallis nonparametric test showed significant differences among the groups, while the multiple comparison test showed that the IRM group was statistically better than the gutta percha group when the average lengths of time for contamination were compared (P < 0.05).

Effectiveness of the Canal Finder and hand instrumentation in removal of gutta-percha root fillings during root canal retreatment.

The purpose of this study was to quantify the amount of remaining gutta-percha/sealer on the canal walls when the Canal Finder system, hand instrumentation and a combination of both (hybrid technique) were used to remove these materials. The amount of apically-extruded debris and the time required for removal were also recorded. Sixty extracted maxillary anterior teeth were prepared using a stepback technique and obturated with gutta-percha. Reinstrumentation of all groups was done in conjunction with a solvent, chloroform. The teeth were split longitudinally and photographed, and the total area of the root canal and the area of the debris were traced and quantified using a computerized image analysis system. The ratio of remaining obturation material to root canal surface was derived and analysed statistically. Hand instrumentation resulted in less debris remaining than did the other two techniques (P < 0.05). The differences in the amount of apically extruded debris were not significant among the techniques (P > 0.05). The hybrid technique required significantly less time for filling material removal. The Canal Finder system alone was not superior to hand instrumentation.

Factors associated with endodontic flare-ups: a prospective study.

The purpose of this prospective study was to assess the incidence of flare-ups (a severe problem requiring an unscheduled visit and treatment) among patients who received endodontic treatment by the two authors in their respective practices during a period of one year, and also to examine the correlation with pre-operative and operative variables. The results showed an incidence of 1.58% for flare-ups from 1012 endodontically treated teeth. Statistical analysis using the chi-square test (P<0.05) indicated that flare-ups were found to be positively correlated with multiple appointments, retreatment cases, periradicular pain prior to treatment, presence of radiolucent lesions, and patients taking analgesic or anti-inflammatory drugs. In contrast, there was no correlation between flare-up, and age, sex, different arch/tooth groups and the status of the pulp.

Guided bone regeneration in the treatment of a large periapical lesion: a case report.

A large (19 mm x 24 mm) periapical lesion associated with tooth #10, perforating both the labial and palatal cortical plates, was treated with decalcified freeze-dried bone allograft and guided bone regeneration. The membranes were removed after a seven-month healing period. Complete osseous reconstruction of the cortical plates was noted. Biopsies demonstrated healing with mature bone and hemopoietic marrow. Few particles of decalcified freeze-dried bone could be observed. Graft particles that remained were surrounded by new bone. The learning objective of this article is to present the regeneration procedure utilized along with the histologic biopsy results to the clinician.

Ridge augmentation following extraction of replanted central incisor.

Regeneration of bone defects remains a clinically important problem in many instances. This paper reports on the use of membrane technique combined with decalcified freeze-dried bone allograft to promote bone formation after extraction of a replanted central incisor with extensive root resorption. The procedure prevented ridge reduction, both buccolingually and apicocoronally, and will facilitate prosthetic replacement of the lost tooth without esthetic problems.

Endodontic retreatment of thermafil or lateral condensation obturations in post space prepared teeth.

The purpose of this study was to quantify the amount of remaining gutta-percha/sealer after retreatment of post space prepared teeth obturated with a lateral condensation technique or with Thermafil with plastic and metallic carrier. The time required for retreatment was also recorded. Forty-five extracted mandibular premolars were prepared using a step-back flared technique and obturated. The post space was prepared and a 5-mm obturation was left in the canal. Retreatment of all groups was done using a solvent technique. The teeth were split longitudinally and photographed. The total area of the canal and the area of gutta-percha/sealer were traced on white paper. Both areas were quantified using a computerized image analysis system and the ratio of remaining obturation material to root canal periphery was derived and statistically analyzed. Statistical analysis (analysis of variance, p = 0.01) showed no differences among the techniques when the average percentage of remaining gutta-percha/sealer was compared. Results revealed that the Thermafil metallic retreatment group consumed significantly more time than the others (analysis of variance, Scheffe p = 0.01). The metallic carriers were not easily removed from the canals, six metal carriers could not be retrieved during the retreatment routine.